CN118451353A - 显微镜和用于显微镜检查的方法 - Google Patents

显微镜和用于显微镜检查的方法 Download PDF

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Publication number
CN118451353A
CN118451353A CN202280084832.9A CN202280084832A CN118451353A CN 118451353 A CN118451353 A CN 118451353A CN 202280084832 A CN202280084832 A CN 202280084832A CN 118451353 A CN118451353 A CN 118451353A
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CN
China
Prior art keywords
sample
camera
illumination
partial
image
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202280084832.9A
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English (en)
Chinese (zh)
Inventor
T·安胡特
D·施韦特
M·瓦尔德
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carl Zeiss Microscopy GmbH
Original Assignee
Carl Zeiss Microscopy GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carl Zeiss Microscopy GmbH filed Critical Carl Zeiss Microscopy GmbH
Publication of CN118451353A publication Critical patent/CN118451353A/zh
Pending legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/18—Arrangements with more than one light path, e.g. for comparing two specimens
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/361—Optical details, e.g. image relay to the camera or image sensor
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Engineering & Computer Science (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CN202280084832.9A 2021-12-22 2022-11-21 显微镜和用于显微镜检查的方法 Pending CN118451353A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102021134427.1A DE102021134427A1 (de) 2021-12-22 2021-12-22 Mikroskop und verfahren zur mikroskopie
DE102021134427.1 2021-12-22
PCT/EP2022/082544 WO2023117236A1 (de) 2021-12-22 2022-11-21 Mikroskop und verfahren zur mikroskopie

Publications (1)

Publication Number Publication Date
CN118451353A true CN118451353A (zh) 2024-08-06

Family

ID=84439851

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202280084832.9A Pending CN118451353A (zh) 2021-12-22 2022-11-21 显微镜和用于显微镜检查的方法

Country Status (6)

Country Link
US (1) US20250052991A1 (https=)
EP (1) EP4453627B1 (https=)
JP (1) JP2025502743A (https=)
CN (1) CN118451353A (https=)
DE (1) DE102021134427A1 (https=)
WO (1) WO2023117236A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102023005252A1 (de) * 2023-12-19 2025-06-26 Carl Zeiss Microscopy Gmbh Mikroskop mit schneller quasikonfokaler Detektion
DE102024108046A1 (de) 2024-03-20 2025-09-25 Carl Zeiss Microscopy Gmbh Superauflösendes Mikroskop mit schneller quasikonfokaler Detektion
DE102024118478A1 (de) * 2024-06-29 2025-12-31 Carl Zeiss Microscopy Gmbh Mikroskop, slidereader und verfahren zur mikroskopie
DE102024118477A1 (de) * 2024-06-29 2025-12-31 Carl Zeiss Microscopy Gmbh Mikroskop, slidereader und verfahren zur mikroskopie

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101031837B (zh) 2004-07-23 2011-06-15 通用电气医疗集团尼亚加拉有限公司 用于荧光共焦显微镜检查的方法和设备
GB0619636D0 (en) 2006-10-05 2006-11-15 Cairn Res Ltd Optical imaging device
DE102009060793A1 (de) 2009-12-22 2011-07-28 Carl Zeiss Microlmaging GmbH, 07745 Hochauflösendes Mikroskop und Verfahren zur zwei- oder dreidimensionalen Positionsbestimmung von Objekten
WO2012002893A1 (en) * 2010-06-30 2012-01-05 Ge Healthcare Bio-Sciences Corp A system for synchronization in a line scanning imaging microscope
WO2013106731A1 (en) 2012-01-11 2013-07-18 Howard Hughes Medical Institute Multi-dimensional imaging using multi-focus microscopy
DE102014002328B4 (de) 2014-02-12 2021-08-05 Carl Zeiss Microscopy Gmbh Multifokales Fluoreszenzrastermikroskop
US11231570B2 (en) * 2018-04-30 2022-01-25 University Of Central Florida Research Foundation, Inc. Highly inclined swept tile (HIST) imaging apparatus, methods, and applications
EP3614190A1 (en) * 2018-08-20 2020-02-26 Till GmbH Microscope device with virtual objective

Also Published As

Publication number Publication date
JP2025502743A (ja) 2025-01-28
WO2023117236A1 (de) 2023-06-29
DE102021134427A1 (de) 2023-06-22
EP4453627A1 (de) 2024-10-30
US20250052991A1 (en) 2025-02-13
EP4453627B1 (de) 2026-02-18

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