DE102005027407B3 - Thermo cycler, for polymerase chain reactions, comprises a cover over the holding zone for the reaction vessels with a sealing wall adjusted longitudinally by an external setting unit - Google Patents
Thermo cycler, for polymerase chain reactions, comprises a cover over the holding zone for the reaction vessels with a sealing wall adjusted longitudinally by an external setting unit Download PDFInfo
- Publication number
- DE102005027407B3 DE102005027407B3 DE102005027407A DE102005027407A DE102005027407B3 DE 102005027407 B3 DE102005027407 B3 DE 102005027407B3 DE 102005027407 A DE102005027407 A DE 102005027407A DE 102005027407 A DE102005027407 A DE 102005027407A DE 102005027407 B3 DE102005027407 B3 DE 102005027407B3
- Authority
- DE
- Germany
- Prior art keywords
- wall
- lid
- reaction vessels
- holding zone
- working position
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0689—Sealing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/141—Preventing contamination, tampering
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/142—Preventing evaporation
Abstract
Description
Die Erfindung bezieht sich auf einen Thermocycler.The The invention relates to a thermal cycler.
Mit dem Begriff Thermocycler werden generell Einrichtungen bezeichnet, in denen eine PCR-Reaktion durchgeführt werden kann. Bei PCR handelt es sich um eine etablierte, Fachleuten bekannte Abkürzung für ein Verfahren, in dem mit einer Polymerase gezielt in einer Probe enthaltene Target-Nukleinsäuren amplifiziert werden könnnen. Ein Beispiel für einen Thermocycler zeigt z.B. die WO01/08801 A1.With The term thermal cycler generally refers to devices, in which a PCR reaction can be carried out. When PCR is it is an established abbreviation for a method known to those skilled in the art, amplified in the targeted with a polymerase contained in a sample target nucleic acids can be. An example for a thermocycler shows e.g. WO01 / 08801 A1.
Die
Erfindung betrifft spezielle Thermocycler, mit denen im Real-Time-Modus
die Zunahme der amplifizierten Produkte über die Zeit gemessen werden
kann. Solche Thermocycler weisen einen Basiskörper auf, in dem ein für die Durchführung von PCR-Reaktionen
geeignet temperierbarer Aufnahmebereich für Reaktionsgefäße ausgebildet
ist. Weiterhin ist ein Deckel vorgesehen, der aus einer geöffneten
Position in eine Arbeitsposition über dem Aufnahmebereich verfahren werden
kann. An dem Deckel ist eine optische Meßeinrichtung angeordnet, die in
Arbeitsposition dem Aufnahmebereich gegenüberliegt und optische Signale
in den Reaktionsgefäßen detektieren
kann. Ein derartiger Cycler ist z.B. aus der
Grundsätzlich ist bei PCR-Reaktionen eine exakte Temperierung der Reaktionsgefäße außerordentlich wichtig. Dies gilt insbesondere für Real-Time Verfahren, da mit diesen Verfahren in aller Regel auch quantifiziert wird und schon geringe Abweichungen von dem Soll-Temperaturverlauf zu falschen Ergebnissen führen können. Partielle Temperaturschwankungen durch Nebenlufteinflüsse müssen daher ausgeschlossen werden. Gleiches gilt für seitlich in den Lichtweg zwischen optischer Meßeinrichtung und Aufnahmebereich einfallendes Licht, das die Messung beeinträchtigen könnte.Basically in PCR reactions an exact temperature control of the reaction vessels extraordinarily important. This is especially true for real-time procedures, as with This method is also usually quantified and already small deviations from the target temperature profile to wrong Results can. Partial temperature fluctuations due to side-effects must therefore be excluded. The same applies to the side in the light path between optical measuring device and receiving area incident light that affect the measurement could.
Der
aus der
Nachteilig an dem bekannten Thermocycler ist, dass Deckel und Wand ein Bauteil bilden. Sobald der Deckel in die Arbeitsposition verbracht ist, erfolgt daher eine automatische Abschirmung des Aufnahmebereichs, der dann nicht mehr von aussen zugänglich ist, ohne dass der gesamte relativ schwere Deckel bewegt wird.adversely on the known thermal cycler is that the lid and wall a component form. Once the lid has been moved to the working position, Therefore, an automatic shielding of the recording area, which is then no longer accessible from outside, without the entire relatively heavy lid is moved.
Aufgabe der Erfindung ist es daher, einen Thermocycler bereitzustellen, der ein einfacheres Handling erlaubt.task The invention therefore is to provide a thermal cycler, which allows easier handling.
Gelöst wird die Aufgabe durch einen Thermocycler, der die Merkmale des Anspruches 1 aufweist.Is solved the task by a thermal cycler, the features of the claim 1 has.
Wie aus dem Stand der Technik bekannt ist auch in dem erfindungsgemäßen Thermocycler eine den Aufnahmebereich umlaufend ausgebildete, sich zwischen Deckel und Basiskörper erstreckende gas- und lichtdichte Wand angeordnet, die gegen Deckel und Basiskörper abgedichtet ist und mit der man eine effektive Abschirmung des Aufnahmebereiches erreicht, in dem die PCR-Reaktion stattfindet, vor z.B. Nebenlufteffekten.As known from the prior art is also in the thermal cycler according to the invention a receiving area circumferentially formed between itself cover and base body extending gas- and light-tight wall arranged against the lid and base body is sealed and with the one effective shielding of the receiving area reached in which the PCR reaction takes place before e.g. In addition to air effects.
Die Wand umgibt in Arbeitsposition des Deckels sowohl den Aufnahmebereich im Basiskörper als auch die optische Messeinrichtung im Deckel, so dass ein nach aussen abgeschirmter Lichtweg und Reaktionsraum entsteht.The Wall surrounds both the receiving area in the working position of the lid in the base body as also the optical measuring device in the lid, so that one outwards Shielded light path and reaction space is formed.
Die Wand ist als mit dem Thermocycler verbundenes Bauteil ausgebildet, wobei erfindungsgemäß die Wand ein in seiner Länge mit einer Stelleinrichtung variabel einstellbarer Faltenbalg ist.The Wall is designed as a component connected to the thermal cycler, wherein according to the invention the wall one in its length with a setting device is variably adjustable bellows.
In einer bevorzugten Ausgestalung kann der Faltenbalg mit seinem einen offenen Ende z.B. umlaufend am Deckel befestigt sein und mit seinem freien offenen Ende mittels der Stelleinrichtung in Arbeitsposition des Deckels bis auf den Basiskörper abgesenkt werden.In In a preferred embodiment, the bellows with its one open end e.g. be attached to the lid all around and with his free open end by means of the actuator in working position of the lid down to the base body be lowered.
Um eine optimale Dichtung zu erreichen, kann an dem freien offenen Ende des Faltenbalgs eine oder mehrere umlaufende Dichtungen, z.B. aus Silikon oder dergleichen, angeordnet sein.Around To achieve an optimal seal, can be at the open open One or more circumferential seals, e.g. made of silicone or the like.
Die Betätigung des Faltenbalgs erfolgt über eine Stelleinrichtung. Dies kann eine separat am Deckel vorgesehene Einrichtung, z.B. ein Stellrad oder dergleichen sein. Sofern an dem Deckel bereits Betätigungseinrichtungen angeordnet sind, mit denen z.B. eine Verstellung des Deckels bzw. eine Verriegelung in Arbeitsposition erfolgt, kann die Betätigung der verstellbaren Wand auch über diese Einrichtung erfolgen. Man könnte dann z.B. die Betätigungen derart synchronisieren, daß bei Verfahren des Deckels und Verriegeln in der Arbeitsposition mit der dafür vorgesehenen Betätigungseinrichtung gleichzeitig Wand zwischen Deckel und Basisstation ausgefahren wird.The activity the bellows is done via a Setting device. This may be a device provided separately on the lid, e.g. a thumb wheel or the like. If already on the lid actuators are arranged with which e.g. an adjustment of the lid or a lock is made in working position, the actuation of the adjustable wall also over this device is done. One could then, e.g. the activities Synchronize such that at Procedure of the lid and locking in the working position with the designated actuator at the same time wall between cover and base station is extended.
Es versteht sich, daß obige Ausführungen nur exemplarisch sind. Selbstverständlich kann die Wand auch im bzw. am Basiskörper angeordnet sein.It is understood that the above statements are only exemplary. Of course, the Wall also be arranged in or on the base body.
Der Thermocycler soll im Folgenden anhand einer Figur näher erläutert werden.Of the Thermocycler will be explained in more detail below with reference to a figure.
In
der Figur erkennt man einen schematisch und im Schnitt dargestellte
Thermocycler
Der
Thermocycler
Zwischen
Deckel
Claims (3)
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102005027407A DE102005027407B3 (en) | 2005-06-13 | 2005-06-13 | Thermo cycler, for polymerase chain reactions, comprises a cover over the holding zone for the reaction vessels with a sealing wall adjusted longitudinally by an external setting unit |
US11/916,616 US20080210700A1 (en) | 2005-06-13 | 2006-03-04 | Thermocycler |
PCT/EP2006/001996 WO2006133750A1 (en) | 2005-06-13 | 2006-03-04 | Thermocycler |
CA002610732A CA2610732A1 (en) | 2005-06-13 | 2006-03-04 | Thermocycler |
EP06707420A EP1890816A1 (en) | 2005-06-13 | 2006-03-04 | Thermocycler |
CN2006800211735A CN101198408B (en) | 2005-06-13 | 2006-03-04 | Thermo cycler |
JP2008516142A JP2008543292A (en) | 2005-06-13 | 2006-03-04 | Thermocycler equipment |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102005027407A DE102005027407B3 (en) | 2005-06-13 | 2005-06-13 | Thermo cycler, for polymerase chain reactions, comprises a cover over the holding zone for the reaction vessels with a sealing wall adjusted longitudinally by an external setting unit |
Publications (1)
Publication Number | Publication Date |
---|---|
DE102005027407B3 true DE102005027407B3 (en) | 2006-11-09 |
Family
ID=36570726
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE102005027407A Expired - Fee Related DE102005027407B3 (en) | 2005-06-13 | 2005-06-13 | Thermo cycler, for polymerase chain reactions, comprises a cover over the holding zone for the reaction vessels with a sealing wall adjusted longitudinally by an external setting unit |
Country Status (7)
Country | Link |
---|---|
US (1) | US20080210700A1 (en) |
EP (1) | EP1890816A1 (en) |
JP (1) | JP2008543292A (en) |
CN (1) | CN101198408B (en) |
CA (1) | CA2610732A1 (en) |
DE (1) | DE102005027407B3 (en) |
WO (1) | WO2006133750A1 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102014108143A1 (en) * | 2014-06-10 | 2015-12-10 | Kist Europe-Korea Institute of Science and Technologie Europe Forschungsgesellschaft mbh | An optical system for detecting fluorescent or luminescent signals of at least two samples |
DE102016200271A1 (en) * | 2016-01-13 | 2017-07-13 | Institut Dr. Foerster Gmbh & Co. Kg | Device for generating and measuring an emission |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102005027555B3 (en) * | 2005-06-14 | 2006-10-05 | Eppendorf Ag | Thermocycler for carrying out polymerase chain reactions, has thermostatically controlled area, in which reaction vessel is placed, lid being placed over this incorporating an optical unit adjusted using pins on base and sleeves on lid |
EP2359933B1 (en) * | 2007-02-13 | 2017-11-08 | Eppendorf AG | Cover for sample with sample-size independent height adjustment |
EP2364777B1 (en) | 2007-02-13 | 2018-07-25 | Eppendorf AG | Process for controlling the temperature of samples |
US8247217B2 (en) | 2008-02-15 | 2012-08-21 | Bio-Rad Laboratories, Inc. | Thermal cycler with self-adjusting lid |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998020965A1 (en) * | 1996-11-13 | 1998-05-22 | Doebelin Werner | Reaction chamber system for chemical synthesis or related applications |
WO1999060381A1 (en) * | 1998-05-16 | 1999-11-25 | The Perkin-Elmer Corporation | Instrument for monitoring polymerase chain reaction of dna |
WO2001008801A1 (en) * | 1999-07-30 | 2001-02-08 | Stratagene | Apparatus for thermally cycling samples of biological material |
US6238627B1 (en) * | 1998-08-26 | 2001-05-29 | Arqule, Inc. | Reaction block and cover |
DE10131687A1 (en) * | 2001-06-29 | 2003-01-16 | Eppendorf Ag | Device for carrying out nucleic acid amplification reactions while simultaneously monitoring the formation of amplification products |
US20040018610A1 (en) * | 2002-07-23 | 2004-01-29 | Sandell Donald R. | Slip cover for heated platen assembly |
DE10232202A1 (en) * | 2002-07-16 | 2004-02-05 | Herz, Helmut, Dr.-Ing. | Sample treatment station |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100236506B1 (en) * | 1990-11-29 | 2000-01-15 | 퍼킨-엘머시터스인스트루먼츠 | Apparatus for polymerase chain reaction |
JPH0523900U (en) * | 1991-09-13 | 1993-03-30 | 公和 池亀 | Indicators for food hygiene |
JP2001337039A (en) * | 2000-05-26 | 2001-12-07 | Dkk Toa Corp | Emission detector |
DE10046224A1 (en) * | 2000-09-19 | 2002-03-28 | Merck Patent Gmbh | Unit for parallel incubation of solutions includes frame holding titration plate closely against cover plate, forming tight seal |
JP2004534226A (en) * | 2001-06-29 | 2004-11-11 | メソ スケイル テクノロジーズ,エルエルシー | Assay plate, reader system and method for luminescence test measurement |
JP3811393B2 (en) * | 2001-11-28 | 2006-08-16 | 松下電器産業株式会社 | Fluorescence detection device |
DE10205977A1 (en) * | 2002-02-08 | 2003-08-28 | Eppendorf Ag | Robotic workstation for the preparation of microtitration plates, especially for PCR assays, comprises a heating/cooling unit to set the sample temperature and, optionally, an automatic plate sealing function |
JP2005523440A (en) * | 2002-04-19 | 2005-08-04 | パムジーン ベー.ベー. | System, substrate plate and incubation apparatus for performing a bioassay |
AU2003240772A1 (en) * | 2002-04-19 | 2003-11-03 | Pamgene B.V. | Substrate plate, method and apparatus for manufacturing such a substrate plate, and system for conducting biossays comprising such a substrate plate |
-
2005
- 2005-06-13 DE DE102005027407A patent/DE102005027407B3/en not_active Expired - Fee Related
-
2006
- 2006-03-04 US US11/916,616 patent/US20080210700A1/en not_active Abandoned
- 2006-03-04 JP JP2008516142A patent/JP2008543292A/en active Pending
- 2006-03-04 WO PCT/EP2006/001996 patent/WO2006133750A1/en active Application Filing
- 2006-03-04 EP EP06707420A patent/EP1890816A1/en not_active Withdrawn
- 2006-03-04 CN CN2006800211735A patent/CN101198408B/en not_active Expired - Fee Related
- 2006-03-04 CA CA002610732A patent/CA2610732A1/en not_active Abandoned
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998020965A1 (en) * | 1996-11-13 | 1998-05-22 | Doebelin Werner | Reaction chamber system for chemical synthesis or related applications |
WO1999060381A1 (en) * | 1998-05-16 | 1999-11-25 | The Perkin-Elmer Corporation | Instrument for monitoring polymerase chain reaction of dna |
US6238627B1 (en) * | 1998-08-26 | 2001-05-29 | Arqule, Inc. | Reaction block and cover |
WO2001008801A1 (en) * | 1999-07-30 | 2001-02-08 | Stratagene | Apparatus for thermally cycling samples of biological material |
US6657169B2 (en) * | 1999-07-30 | 2003-12-02 | Stratagene | Apparatus for thermally cycling samples of biological material with substantial temperature uniformity |
DE10131687A1 (en) * | 2001-06-29 | 2003-01-16 | Eppendorf Ag | Device for carrying out nucleic acid amplification reactions while simultaneously monitoring the formation of amplification products |
DE10232202A1 (en) * | 2002-07-16 | 2004-02-05 | Herz, Helmut, Dr.-Ing. | Sample treatment station |
US20040018610A1 (en) * | 2002-07-23 | 2004-01-29 | Sandell Donald R. | Slip cover for heated platen assembly |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102014108143A1 (en) * | 2014-06-10 | 2015-12-10 | Kist Europe-Korea Institute of Science and Technologie Europe Forschungsgesellschaft mbh | An optical system for detecting fluorescent or luminescent signals of at least two samples |
DE102016200271A1 (en) * | 2016-01-13 | 2017-07-13 | Institut Dr. Foerster Gmbh & Co. Kg | Device for generating and measuring an emission |
US10605732B2 (en) | 2016-01-13 | 2020-03-31 | Institut Dr. Foerster Gmbh & Co. Kg | Portable device for detecting explosive substances comprising a device for generating and measuring the emission of an indicator |
Also Published As
Publication number | Publication date |
---|---|
CA2610732A1 (en) | 2006-12-21 |
US20080210700A1 (en) | 2008-09-04 |
JP2008543292A (en) | 2008-12-04 |
EP1890816A1 (en) | 2008-02-27 |
WO2006133750A1 (en) | 2006-12-21 |
CN101198408A (en) | 2008-06-11 |
CN101198408B (en) | 2010-05-19 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE102005027407B3 (en) | Thermo cycler, for polymerase chain reactions, comprises a cover over the holding zone for the reaction vessels with a sealing wall adjusted longitudinally by an external setting unit | |
DE102014014895A1 (en) | Method and device for producing components in a layer construction method | |
DE2849240C2 (en) | CVD coating device for small parts and their use | |
EP1269243A2 (en) | In-situ microscope device for reactors | |
DE102010008296A1 (en) | Laser processing system, object holder and laser processing method | |
DE102018007307A1 (en) | Pen lifter | |
DE102010053979A1 (en) | Hearth furnace multiple superposed heated planes for reception of flat or partially deformed steel plates, where each plane has two heating units | |
EP4009357A1 (en) | Vacuum clamping device for clamping workpieces, measuring equipment and methods for testing workpieces, in particular wafers | |
DE102005045718B4 (en) | Carrier for a substrate | |
DE102010060824A1 (en) | Device for handling slides | |
DE202011051992U1 (en) | A workplace arrangement | |
DE10246282A1 (en) | Substrate testing apparatus, has substrate carrier to receive and hold substrate in releasable thermal contact, and directly cooled thermal radiation shield protecting substrate from thermal radiation | |
DE102012004505B3 (en) | Pyrometer pipe for measuring temperatures at measurement point within chambers in vacuum treatment plant, comprises a pyrometer having a specific end which is set with a transparent partition wall as particle shield, in measuring range | |
DE102010042777A1 (en) | Laser cutting arrangement comprises first laser cutting unit enclosed by a impermeable cabin for laser beams exhibiting a lock for introducing sheet metal molded parts to be processed, and a second laser cutting unit within the lock | |
DE102016110170B3 (en) | Galvanizing furnace and method for operating a galvanizing furnace | |
DE202016101637U1 (en) | Reaction tank system with rotor element | |
DE102009000305A1 (en) | Cooking apparatus i.e. baking-oven, has protection unit forming protective area for sliding unit, where protection unit is partially arranged outside of protective area and formed as single piece with muffle unit | |
WO2018146127A1 (en) | Method and device for placing a fill line on a vessel | |
DE102014002087A1 (en) | Spectroscopic gas analyzer and method therefor | |
DE102015114129A1 (en) | Device for removing a test contact of a test contact arrangement | |
BE1030510B1 (en) | Cooking device and method of opening | |
DE19518361C1 (en) | Vacuum-tight reaction vessel with a stuffing box for steel processing | |
DE10203070A1 (en) | Ultra-high vacuum tribometer for measurement of friction and wear can be used in ultra-high vacuum conditions and with varying velocity profiles and is also suitable for use with samples of irregular shape | |
DE19506503C1 (en) | Laboratory furnace esp. for research into materials | |
DE1583343B2 (en) | DEVICE FOR GLOWING AND DETECTING OBJECTS |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
8100 | Publication of patent without earlier publication of application | ||
8364 | No opposition during term of opposition | ||
R082 | Change of representative |
Representative=s name: MEISSNER BOLTE & PARTNER GBR, DE Representative=s name: MEISSNER, BOLTE & PARTNER GBR, DE |
|
R119 | Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee | ||
R119 | Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee |
Effective date: 20150101 |