CN101198408A - Thermo cycler - Google Patents

Thermo cycler Download PDF

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Publication number
CN101198408A
CN101198408A CNA2006800211735A CN200680021173A CN101198408A CN 101198408 A CN101198408 A CN 101198408A CN A2006800211735 A CNA2006800211735 A CN A2006800211735A CN 200680021173 A CN200680021173 A CN 200680021173A CN 101198408 A CN101198408 A CN 101198408A
Authority
CN
China
Prior art keywords
top cover
thermo cycler
wall
matrix
housing region
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CNA2006800211735A
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Chinese (zh)
Other versions
CN101198408B (en
Inventor
H·塔施
B·克吕格
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
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Eppendorf SE
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Filing date
Publication date
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Publication of CN101198408A publication Critical patent/CN101198408A/en
Application granted granted Critical
Publication of CN101198408B publication Critical patent/CN101198408B/en
Expired - Fee Related legal-status Critical Current
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/141—Preventing contamination, tampering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/142—Preventing evaporation

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Optical Measuring Cells (AREA)

Abstract

Thermocycler having a base body, which has a temperature-controllable receiving region for reaction vessels, and having a lid which can be brought into a working position over the receiving region, wherein, in the working position of the lid, a gas-tight wall is provided extending between lid and base body and configured so as to surround the receiving region, which wall is sealed off with respect to lid and base body.

Description

Thermo cycler
Technical field
The present invention relates to a kind of by claim 1 thermo cycler as described in the preamble.
Background technology
The common equipment that can carry out the PCR reaction therein is called " thermo cycler ".PCR is a kind of method, that delivered, that the professional person the is known and abbreviation that can directly understand, the target nucleic acid that can increase targetedly and comprise in a sample by polymerase in the method.
Particularly, the present invention relates to thermo cycler, can under real-time mode, measure the increment of the nucleic acid of amplification along with the time by this thermo cycler.This thermo cycler has a matrix, but constitutes one for implementing the housing region that is used for reaction vessel that the suitable constant temperature of PCR reaction is handled in matrix.A top cover is set in addition, and this top cover can move to the operating position above housing region from a unlimited position.An optical measurement device is set on top cover, and it is mutually opposed with housing region on the operating position and can survey optical signalling in reaction vessel.
In principle, extremely important in PCR reaction is the precise dose control of reaction vessel, and this is particularly suitable for real-time method, because also be quantized usually by described method, and the small deviation of the rated temperature curve result that can lead to errors.Therefore must get rid of the temperature fluctuation of the part of causing by the extraneous air effect.Be suitable for side direction equally and be injected into light between optical measuring device and the housing region along light path, described light can influence measurement.
Summary of the invention
The objective of the invention is to, a kind of thermo cycler is provided, under real-time mode, can not be subjected to extraneous factor to implement PCR as far as possible especially by this thermo cycler with influencing and react in simpler and more reliable mode.
This purpose realizes by a kind of thermo cycler with feature of claim 1 characteristic.
Thus, in by thermo cycler of the present invention, be provided with one around housing region airtight wall that constitute, that between top cover and matrix, extend, described wall top pressure closure and matrix.
By realizing making housing region avoid for example influence of extraneous air effect (Nebenlufteffekten) effectively, in this housing region, carry out the PCR reaction by the wall of setting of the present invention.
On traditional thermo cycler, also wall of the present invention can be set in principle.But preferably it can be provided with on the thermo cycler of working under the real-time mode.In this case, described wall also is lighttight except air-tightness and not only surrounds housing region in the matrix and also surround optical measuring device on the top cover on the operating position of top cover, thereby forms light path and the PCR reaction compartment that not influenced by external factor.
Possible in theory is, provides described wall as parts independently, and it was arranged on the matrix before the PCR reaction, and the end with the opening on its top realizes rabbeting with top seal ground when for example top cover drops to the operating position.
Yet suitable manner is more, and wall is set to the member that is connected with thermo cycler, is contemplated that, described wall is set as the outstanding member of the rigidity on matrix or top cover.Yet have the danger of influence operation in this case, it is therefore preferable that wall only when implementing the PCR reaction just to protrude in top cover or matrix up or down for the essential length of sealing.Be contemplated that for example wall integral body under unwanted situation is moved in top cover or the matrix.
Another one of the present invention is preferred structural specifications in framework of the present invention, and wall has the height that can change adjusting.This for example realizes in a particularly preferred structure in this wise, that is: wall is made into bellows, described wall for example be fixed on the top cover with an end of its opening around ground and with its freely an end of opening can drop on the matrix in the operating position of top cover by a suitable conditioning equipment.In order to realize good sealing, can set at this, on the openend freely of wall or the preferred bellows that uses, be provided with one or more around the seal of making by silicones etc.Self-evidently be.Such seal also can be arranged on the end of two openings of described wall in other cases, if when relating to an independent element.
The operation of bellows can be for example via for example realization such as regulating wheel of an equipment that on top cover, is provided with separately.If on top cover, be provided with an operating equipment, for example realize the adjusting of top cover and the locking in the operating position by this operating equipment, the operation of adjustable wall also realizes via described conditioning equipment.So for example operation can be carried out so synchronously, that is to say, when moving top cover and being locked in the operating position, be moved out to simultaneously between top cover and the matrix location by the wall of setting of the present invention by the operating means that is provided with for this reason.
Will be understood that above-mentioned embodiment only is exemplary.Certainly, described wall also can be arranged in the matrix or on matrix.As long as the length of sealed wall can be regulated, might not be designed to bellows.Other length-adjustable structure example such as stepped construction, stretching structure also are admissible.
Description of drawings
Below illustrate in greater detail the present invention by a diagrammatic sketch.
The specific embodiment
In this diagrammatic sketch, as can be seen one schematically and with the thermo cycler shown in the cutaway view 10, described thermo cycler 10 has a matrix 11, a housing region 12 that is used for reaction vessel is set on this matrix 11.For the temperature of regulating housing region 12 is provided with heater 13, especially a Peltier (Peltier) element 14.Schematically illustrate a groove 15, in this groove, can pack into and wait to regulate the reaction vessel of temperature.Usually housing region is designed for holding the droplet price fixing on thermo cycler, and this does not show in diagrammatic sketch.
Thermo cycler 10 has one at the top cover shown in the operating position 18 in addition, and this top cover is lying against on the described position on the projection 19 that is provided with on the matrix 11.Be provided with on the top cover 18 and under see and be provided with an optical measurement device 20 that this measurement mechanism has the instrument connection 21 that respectively is equipped on accommodation section 15.Can distinguish the increment that optical measurement takes place by optical measurement device 20 in reaction vessel.
One wall 22 that constitutes bellows (Faltenbalg) is set between top cover 18 and matrix 11, and this wall surrounds optical measurement device 20 and housing region 12.This wall 22 is fixed on the top cover 18 with the one end, can regulate the length of described wall 22 changeably by conditioning equipment 25.Conditioning equipment 25 has regulating element 26, and it can move in the top cover or from top cover 18 along the direction of arrow 29 when the operation conditioning equipment 28 by the mechanism 27 that is not shown specifically and shift out.When being positioned at low level, regulating element 26 with its bottom act on the free edge of wall 22, be provided with around seal 30, it constitutes expansion downwards, so that effectively stop entering of light.When operating equipment 28, therefore described wall energy drops on the matrix 11 from top cover 18 as required, so that sealing perhaps is moved near the resting position top cover 18.

Claims (8)

1. thermo cycler, but comprise a matrix and a top cover with housing region that is used for reaction vessel of constant temperature processing, this top cover can be placed in the operating position of housing region top, it is characterized in that, in the operating position of top cover (18), be provided with one that constitute around housing region (12), between top cover (18) and matrix (11) wall (22) extension, airtight, described wall seals described top cover (18) and described matrix (11).
2. thermo cycler as claimed in claim 1 is characterized in that, on top cover (18), be provided with one in the operating position with the opposed optical measurement device of housing region (12) (20).
3. as one of above claim described thermo cycler, it is characterized in that described wall (22) is lighttight.
4. as one of above claim described thermo cycler, it is characterized in that the vary in length ground of described wall (22) is regulated.
5. as one of above claim described thermo cycler, it is characterized in that described wall (22) is arranged on the top cover (18) with open end, its top.
6. thermo cycler as claimed in claim 5 is characterized in that, goes up setting operation device (28), the length that can regulate described wall (22) by this operating means at top cover (18).
7. as one of above claim described thermo cycler, it is characterized in that described wall (22) is a bellows.
8. thermo cycler as claimed in claim 7 is characterized in that, one or more around seal (30) be arranged on the free end of described wall (22).
CN2006800211735A 2005-06-13 2006-03-04 Thermo cycler Expired - Fee Related CN101198408B (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102005027407A DE102005027407B3 (en) 2005-06-13 2005-06-13 Thermo cycler, for polymerase chain reactions, comprises a cover over the holding zone for the reaction vessels with a sealing wall adjusted longitudinally by an external setting unit
DE102005027407.2 2005-06-13
PCT/EP2006/001996 WO2006133750A1 (en) 2005-06-13 2006-03-04 Thermocycler

Publications (2)

Publication Number Publication Date
CN101198408A true CN101198408A (en) 2008-06-11
CN101198408B CN101198408B (en) 2010-05-19

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN2006800211735A Expired - Fee Related CN101198408B (en) 2005-06-13 2006-03-04 Thermo cycler

Country Status (7)

Country Link
US (1) US20080210700A1 (en)
EP (1) EP1890816A1 (en)
JP (1) JP2008543292A (en)
CN (1) CN101198408B (en)
CA (1) CA2610732A1 (en)
DE (1) DE102005027407B3 (en)
WO (1) WO2006133750A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102005027555B3 (en) * 2005-06-14 2006-10-05 Eppendorf Ag Thermocycler for carrying out polymerase chain reactions, has thermostatically controlled area, in which reaction vessel is placed, lid being placed over this incorporating an optical unit adjusted using pins on base and sleeves on lid
EP1961484B1 (en) 2007-02-13 2016-07-20 Eppendorf Ag Cover for sample with homogeneous pressure application
EP1964609B1 (en) * 2007-02-13 2018-07-18 Eppendorf Ag Process for covering samples with sample-size independent height adjustment
US8247217B2 (en) * 2008-02-15 2012-08-21 Bio-Rad Laboratories, Inc. Thermal cycler with self-adjusting lid
DE102014108143A1 (en) * 2014-06-10 2015-12-10 Kist Europe-Korea Institute of Science and Technologie Europe Forschungsgesellschaft mbh An optical system for detecting fluorescent or luminescent signals of at least two samples
DE102016200271A1 (en) * 2016-01-13 2017-07-13 Institut Dr. Foerster Gmbh & Co. Kg Device for generating and measuring an emission

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US7560273B2 (en) * 2002-07-23 2009-07-14 Applied Biosystems, Llc Slip cover for heated platen assembly
KR100236506B1 (en) * 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 Apparatus for polymerase chain reaction
JPH0523900U (en) * 1991-09-13 1993-03-30 公和 池亀 Indicators for food hygiene
CH688987A5 (en) * 1996-11-13 1998-07-15 Doebelin Werner Reaction chamber for chemical synthesis or related applications.
EP2315002A3 (en) * 1998-05-16 2012-12-05 Life Technologies Corporation Filter module for an optical instrument used in particular for monitoring DNA polymerase chain reactions
US6238627B1 (en) * 1998-08-26 2001-05-29 Arqule, Inc. Reaction block and cover
US6657169B2 (en) * 1999-07-30 2003-12-02 Stratagene Apparatus for thermally cycling samples of biological material with substantial temperature uniformity
JP2001337039A (en) * 2000-05-26 2001-12-07 Dkk Toa Corp Emission detector
DE10046224A1 (en) * 2000-09-19 2002-03-28 Merck Patent Gmbh Unit for parallel incubation of solutions includes frame holding titration plate closely against cover plate, forming tight seal
DE10131687A1 (en) * 2001-06-29 2003-01-16 Eppendorf Ag Device for carrying out nucleic acid amplification reactions while simultaneously monitoring the formation of amplification products
DK1412725T3 (en) * 2001-06-29 2019-03-25 Meso Scale Technologies Llc Multi-well plates for LUMINESCENSE TEST MEASUREMENTS
JP3811393B2 (en) * 2001-11-28 2006-08-16 松下電器産業株式会社 Fluorescence detection device
DE10205977A1 (en) * 2002-02-08 2003-08-28 Eppendorf Ag Robotic workstation for the preparation of microtitration plates, especially for PCR assays, comprises a heating/cooling unit to set the sample temperature and, optionally, an automatic plate sealing function
US20060057032A1 (en) * 2002-04-19 2006-03-16 Blok Herman J Substrate plate, method and apparatus for manufacturing such a substrate plate, and system for conducting biossays comprising such a substrate plate
EP1499441A1 (en) * 2002-04-19 2005-01-26 PamGene B.V. System, substrate plate and incubation device for conducting biossays
DE10232202B4 (en) * 2002-07-16 2005-08-25 H+P Labortechnik Ag Sample treatment station

Also Published As

Publication number Publication date
CN101198408B (en) 2010-05-19
DE102005027407B3 (en) 2006-11-09
WO2006133750A1 (en) 2006-12-21
CA2610732A1 (en) 2006-12-21
US20080210700A1 (en) 2008-09-04
JP2008543292A (en) 2008-12-04
EP1890816A1 (en) 2008-02-27

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Granted publication date: 20100519

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