DD278052C2 - PROCESS FOR IMPROVING THE TREATMENT OF SEED AND PLANT - Google Patents

Description

Workup be obtained, for. B. as Fermentorablauf, as centrifugal, as dry matter from the spray, roll or fluidized bed drying, before or after extraction of Zellinhaltsstoffen. The microbial biomass are primarily available as aqueous suspensions of varying concentration. These suspensions (10-1 OOgBTS / 1) can be mixed directly as a formulating agent with the active ingredients. When using dry material, whose particular advantage is its simple and almost unlimited shelf life, 10-80Og of biomass (BTS) are mixed with 11 water. The addition of active ingredients or finished pesticide preparations or other admixtures takes place in different ways. An active ingredient can be mixed in solid or dissolved form with the biomass. Subsequently, the preparation of the aqueous suspension. In any case, the treatment of the seed or planting material of mixing with the aqueous suspension. The application rate is depending on the type of plant, the seed size, etc. 0.15 to ΙΟΙ / dt seed or seedlings.

The use of microbial biomass as a formulation, adhesive or binder has the advantage that such biomass represents a complex of substances that has a pronounced affinity for very different active ingredients and nutrients as well as for the surface of seeds and other parts of plants. Its composition depends on the fermentation conditions and can be changed according to the requirements. Above all, the content of cell polymers is important for the use according to the invention, especially protein (20-60%), polysaccharides (10-30%), lipids (5-80%). In special cases where the biomass has its own activities, attachment without admixtures is possible. Another advantage of microbial biomass is that it is degraded by soil microorganisms and thus does not introduce additional foreign matter into the ecosystem. Their ingredients and degradation products are natural products and are more likely to support the effect of the introduced pesticides. In contrast to film-forming polymers, the microbial biomass, which consists of minute particles, is densely but raster-like attached to the seed or plant matter, which benefits the mass transfer with the environment. Biomass can, preferably in connection with fluidized bed process also find application as pilling. By the following examples, the invention will be further explained:

example 1

The fungicide Falisan Universal Trockenbeize 69 is used to treat winter wheat at a rate of 200g / dt seed. The treatment of the seed is carried out such that in a pickling drum 2.5 kg with the aqueous suspension of a mixture of bacterium dry substance and Falisan Universal Trockenbeize 69 thoroughly mixed. The application rate of the suspension is 1 l / dt. 200g of fungicide are mixed with 50-40Og bacterium dry substance and made up to a volume of 11 water. The adhesiveness of the Trockenbeizpräparates and the resulting amount of dust was tested on .14 hours stored in shallow dishes seeded seed by means of model apparatus and the amount of mordant determined gravimetrically. Flowability was determined by measuring the flow rate of 2 kg of treated seed. The microbial biomass was prepared from fermentation effluent by spray drying. The results are summarized in the following table:

Application rate of bacteria detention dust flow dry matter ability amount speed (G / dt) (%) (mg / 2 kg) (s / 2 kg) Methylobacter spec. (PHB-containing) 50 94.5 3.35 14.3 100 96.1 2.82 14.5 200 97.1 2.62 14.8 400 97.9 2.80 15.4 Acetobacter methanolicus 100 95.5 3.85 14.4 200 96.8 3.05 14.5 400 97.8 3.80 14.6

Dry dressing without additives 87,2 6,5 14,5

mixture of microbial

biomass

Example 2

In 1988, a germination and field emergence test of treated maize seed was carried out. The fungicide used was Wolfen-Thiuram 85 (200 g / dt). The seed was treated as described in Example 1 with an aqueous suspension of the mixture of bacterial dry matter and fungicide. The application rate was 2 l / dt. The germination test was carried out according to TGI6779 / 02 (1973) and the field emergence test in small parcels with 4χ 100 grains. The length measurement of root and shoot was made after 7 days of germination at 20 ⁰ C.

The results of this study are shown in the following table:

germination middle length - 3 - 278 052 Application rate root shoot Feldauf Bacteria-TS (%) (cm) (cm) gear (G / dt) (%) untreated 95.5 14.5 6.8 control 75.2 FungizideThiuram 96 5 16.2 a, 2 without BTS 400 ml / dt 83.5 Fungicide thiuram mitBTSAcetobact. 95.3 18.1 10.6 methanolicus200g / dt 82.0

Example 3

The treatment of maize seed with Inerti / d Oftanol T was carried out.

For 1 kg of seed, 4 g of Oftanol T were mixed with 150 mg of PHB-containing bacterial dry substance of Methylobacter spec., Admixed with 50 ml of dichloromethane and stirred for 1 hour. After evaporation of the solvent, the residue was suspended in 10 ml of water and the corn seed was treated therewith. The liability of the deposited funds is 92.9%. In comparison, without the addition of the bacterial dry substance only an adhesion of 70% for Oftanol T in corn is achieved.

Example 4

A mixture of 30g Malipur and 20g PHB-containing bacterial dry substance of Methylobacter spec, is suspended with 100 ml of water. For the treatment of 1 kg seed of field bean 12 ml of this suspension are used. The adhesion of Malipur is then 99.7%. By dressing the broad beans with Malipur without Biomasso addition, however, only an adhesion of about 50% is achieved.

Example 5

This embodiment relates to the fungicidal activity against snow mold (Fusarium nivale) on rye.

The compositions according to the invention were mixed with Falisin Universal Trockenbeize 69 or Falisan CX Universal Trockenbeize and applied after mixing with water. The pickling preparations were used at a rate of 200 g / dt seed.

The test is carried out according to the test methodology for the fungicidal effect of the fasarium plate test, as defined in TGL 31162, Group 14870. The results of the experiments are shown in the following table.

Fiction application rate amount of water Strippers of infestation contemporary Biomassein in ml / dt preparation index medium g / dt seed seeds Untreated control _ - _ 87.0 Untreated control - - Fal.-TB69 23.9 Untreated control - - Fal.-CX 0.8 PHB halt. biomass 150 600 FaI.-TB 69 18.0 biomass 200 800 Fal.-TB69 21.2 Ac. biomass 150 1000 Case T3 69 14.3 Ac. biomass 200 1400 Fal.-TB69 28.9 PHB halt. biomass 150 600 Fal.-CX 0.2 PHB halt. biomass 200 800 Fal.-CX 0 Ac. biomass 150 1U00 Fal.-CX 0 Ac. biomass 200 1400 Fal.-CX 0

Claims (2)

1. A method for improving the treatment of seeds and seedlings with plant protection agents or plant growth or nutrients and / or organisms, characterized in that microbially produced biomass is used as a formulation, adhesive or binder. 2. The method according to claim 1, characterized in that the microbial biomass, native or denatured, mixed with the active substances in solid or dissolved form, or without additives, is suspended in water. Field of application -i <) r invention The invention relates to the treatment of seed or seedlings for the purpose of applying substances which influence the germination and growth of plants or of organisms having the same effect. It can be used in agriculture, forestry and horticulture. Characteristic of the known state of the art For seed and Pflanzgutbehandlung powdered, liquid or pasty formulated fungicides or insecticides are used, which make different demands on the pickling technique and have very different results with respect to the adhesiveness of the agents used. Thus, the use of dry pickling due to the mechanical stress during transport, handling and drilling leads to loss due to abrasion, which amounts to up to 40%. The encrusting in the form of the slurry pickling process only leads to good adhesion values directly after pickling; after drying or after an intermediate storage, the abrasion increases again. As is apparent from the patents DD-PS 102046, DE-OS 2308334, DE-AS 208536, a significantly improved adhesion of the agents to the seed is achieved by the use of natural or synthetic polymers having film-forming properties. These can be combined to achieve an improved adhesive or Pillierfähigkeit with inorganic materials. When shellac, beeswax or synthetic polymers dissolved in organic solvents are used, a complete coating over the seed is generally achieved. Such coatings hinder the exchange of substances, especially swelling and breathing. In addition, synthetic polymers leave non-degradable residues in the soil. Liquid formulated pesticide preparations or adhesives with the addition of organic solvents require a separate removal of the solvent vapors in the treatment of the seed and are unfavorable from a working hygiene point of view. Both these vapors and the dusts resulting from abrasion are, not least because of their content of active ingredients, a loss and a significant environmental impact. Similar disadvantages as for synthetic polymers also apply to B: ilipiclextrakte. They are a mixture of biolipids and hydrocarbons which are extracted from microorganisms after growth on diesel fuels and used as emulsifying or dispersing agents for pesticides (DD-PS 110166) .Other methods assume that bacteria alone do not adhere to and strike seeds Therefore, the treatment with binders, such as carboxymethylcellulose and other components, which are also intended to serve as Nährstoffreservoire ago.This coating is then over-dusted with kaolin to produce the fluidity (DD-PS 260639, DD-PS 260640) .The complexity of this procedure is Dust is inevitable and therefore unsuitable for use in pesticide applications In other processes (EP 0097459, PCT / US 83/01648), bacteria which are to be effective as biofertilisers are only used with additional application. mostly film-forming binders and fillers, attached. Object of the invention The invention has the aim to make the treatment of seed and seedlings so that active ingredients of pesticides, crop protection products and / or other germinating and growth affecting substances or organisms can be attached to abrasion resistant losses and environmental pollution in the treatment, transport - And sowing process to avoid or reduce, and to maintain the mass transfer of the treated seed and planting material with the environment. Essence of the invention The invention has for its object to find a formulation, adhesive or binder, with which hydrophilic and / or hydrophobic agents, nutrients or protective substances and microorganisms can be attached to seeds or seedlings resistant to abrasion. According to the invention, the object is achieved by using microbially produced biomass as the formulation, adhesion or binding agent. As biomass, which are used in this way, come into question: bacteria, eg. B. from processes for SCP production based on methane or methanol, from the Gluconsäureproduktion, from the production of polyhydroxybutyric acid, from the SCP production with yeasts based on diesel fuel, sulfite liquor, molasses, baker's and brewer's yeast, yeast from ethanol and citric acid production or biomass of activated sludge from wastewater treatment. Useful biomass can be from very different stages of fermentation or the