DD212983A1 - ENZYMATIC GAINING OF PROTEIN HYDROLYSATES FOR COSMETIC PURPOSES - Google Patents

Abstract

The invention relates to the enzymatic recovery of protein hydrolysates from natural proteins or proteinaceous. Raw materials for cosmetics. Preparations. Goal d. Invention is to produce protein hydrolysates within desired molecular weight ranges. Invention. is this by d. Use of alkaline serine protease from Thermoactinomyces vulgaris, preferably by protease from Thermoactinomyces vulgaris IMET 9512 u. 9515 reached. The enzymatic hydrolysis is carried out at pH> 7, preferably at pH 8 to 10, and at temperatures up to 80 ° C. When chromium-tanned collagen-containing substrates are used, enzymatic hydrolysis of pH> 8 is expedient.

Description

a) Title of the invention

Enzymatic Production of protein hydrolysates for cosmetic purposes

b) Field of application of the invention

The invention relates to the enzymatic recovery of protein hydrolysates from natural proteins or proteinaceous raw materials for cosmetic preparations, such as creams, bath additives, hair care agents, and the like. a.

c) Characteristic of the known technical solutions

It is known that cosmetic final products containing protein or its degradation products are distinguished by positive properties against skin and hair. Particularly favorable properties are said to be possessed by protein hydrolysates in a molar mass range of from 1 000 to 2 000 daltons.

Protein hydrolysates are prepared by chemical or enzymatic means.

The enzymes mentioned are trypsin, papain and bacterial proteases. The starting substrates used are milk, bi-, cereal and, above all, scleroproteins or proteinaceous raw materials. The enzymatic degradation of the latter usually requires pretreatment. In the described method involves the use of the listed enzymes at a temperature, a st RECOVERY 4 50 ⁰ C. In order to reach the desired molar mass, the hydrolysates su, a longer incubation time is required.

Although enzymatic digestion of protein-containing chrome leather shavings is indeed described (DE-OS 26 43 012 and DE-OS 22 52 281), information on a separation of the liberated chromium ions with simultaneous enzyme reaction were not made.

d) Object of the invention

The aim of the invention is to produce protein hydrolysates of natural proteins and protein-containing soils with the desired properties for cosmetic preparations.

e) Presentation of the essence of the invention

The invention has for its object to carry out the protein hydrolysis with suitable proteases. Thermostable serineprptease from Thermoactinomyces vulgaris is used according to the invention. Protease of Thermoactinomyces vulgaris IMET 9512 and 9515 is preferably used.

The reaction takes place in an aqueous medium at 25 to 80 ⁰ C; in the case of chromium tanned collagen-containing substrates, preferably at 60 ^° C. The pH is> 7, preferably 8 to 10. During enzymatic degradation, pH staging may be carried out. The reaction time depends on the temperature, the enzyme input and the desired molecular weight range of the hydrolysis product. It is between 10 minutes and 24 hours, preferably 3 to 5 hours. The amount of enzyme used depends on the desired degree of hydrolysis of the end product. It is 10 to 100

TE. "/ Ml enzyme / 100 g substrate (enzyme determination according to: U. c as

Behnke u. a., "Lebensmittelindustrie" 2 £, Issue 11, 1978, S, 485). "

Due to the temperature stability, the enzymes act in a wide temperature range. The pH stability of! Enzyme allows working in an alkaline environment, whereby the proposed method for enzymatic ^ Chromlederarbeitarbeitung is particularly suitable.

In the case of processing, the enzyme can be immediately after the thermal-alkaline pretreatment in the cooling phase without temperature adjustment and in the presence of the. Chromium can be added. Chromium released by enzymatic hydrolysis is precipitated by pH stating as chromium hydroside. Ss can be easily separated after termination of the enzymatic treatment.

The process according to the invention leads to uniform product formation. The protein degradation products may have a molecular weight of 500 to 10,000 daltons, depending on the choice of the conditions of the treatment, making them particularly suitable for a wide variety of cosmetic preparations.

f) embodiments

The invention will be explained in more detail by examples.

example 1

100 g of gelatin or bone glue are swollen with 900 ml of tap water for 30 min and dissolved at 40 ⁰ C. The reaction temperature of 40 to 70 ⁰ C (preferably 60 ⁰ C) is set. The pH value of the sample, which is set at about 2 liters, is 8 to 9 (preferably pH 8). Activities of 10 to 100 _TEO / ml of enzyme / 100 g of substrate are added.

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together. ,

During the reaction time of 10 minutes to 24 hours, preferably 3 to 5 hours, is stirred continuously.

Reaction time and enzyme concentration can be varied depending on the required degree of degradation. A desired termination of the Beaktion is achieved by heating the sample to 90 ⁰ C.

After filtration of the sample, the concentration is carried out to 40 to 50% TS in the sample.

A clear, honey-colored product with a narrow molecular weight distribution and a high degree of purity is obtained.

The molecular mass is 500 to 10,000 daltons depending on the conditions of the reaction.

Example 2

Tanned unreported leather waste is added in a ratio of 1:10 (w / v) with a solution of 0.25 to 1 % calcium hydroxide, calcium oxide or sodium hydroxide in tap water and boiled for 20 to 60 minutes, preferably 30 tain. After cooling the batch to 90, the addition of the enzyme takes place.

Cooling of the batch to 90 to 50 ⁰ C, preferably 60 ⁰ C,

The amount of Snzyme used depends on the activity of the enzyme and the desired Hydrolysegräd. It is based on the weight of the substrate, at 10 to 100 ΤΞ _Λ / ml enzyme / 100 g of substrate.

The pH of the batch is kept at 8 to 10, preferably 9, by pH-stating. At this pH, the enzyme is most stable and chromium released by the hydrolysis can be precipitated as chromium hydroxide.

.The reaction temperature is in the range of 25 to 70 ⁰ C, but should preferably be 60 ⁰ C.

The duration of the reaction of the enzymatic reaction is 10 minutes to 24 hours, depending on the desired degree of hydrolysis.

A termination of the enzyme reaction can be achieved by heating the batch above 90 ⁰ C. The workup of the hydrolysates is carried out by the usual methods of separation of the chromium hydroxide and solid constituents by filtration and concentration, spray or freeze-drying,

Example 3

100 g of whey protein are dissolved with 900 si of tap water.

After Teiaperatureeinstellung the pH is adjusted to 8 with sodium hydroxide solution and added 10 to 100 TS ₃₃₃ Thermitase. , "

The hydrolysis is carried out under stirring at 60 ⁰ C.

After the hydrolysis time of 5 hours, the batch for inactivating the enzyme can be heated to 90 ^° C.

Solids, if present, are filtered off and the hydrolyzate is concentrated to a dry matter content of 40 % .

The hydrolyzate has a molecular mass of 500 to 2,000 daltons.

Claims (4)

- 6 Sprinting 1. Enzymatic recovery of protein hydrolysates for cosmetic purposes from natural proteins or protein-containing soups, characterized in that for the hydrolysis alkaline serine protease from Thermoactinomyces vulgaris is used. 2. Enzymatic recovery of protein hydrolysates according to item 1, characterized in that preferably protease of Thermoactinomyces vulgaris TEE]} 9512 and 9515 is used. 3. Enzymatic recovery of protein hydrolysates according to points 1 and 2, characterized in that the Seaktion at pH values> 7, preferably at pH 8 to 10, and carried out at temperatures up to 80 ⁰ C. 4. Enzymatic recovery of protein hydrolysates according to points 1 and 2, characterized in that when using chromium tanned collagen-containing substrates during the enzymatic hydrolysis, a pH-Statierung> 8, preferably to pH 9, carried out and the reaction temperature at 25 to 70 ⁰ C, preferred at 60 ⁰ C, is held.