DD202735A5 - Verfahren zur herstellung invertasefreier alpha-galactosidase - Google Patents
Verfahren zur herstellung invertasefreier alpha-galactosidase Download PDFInfo
- Publication number
- DD202735A5 DD202735A5 DD82240391A DD24039182A DD202735A5 DD 202735 A5 DD202735 A5 DD 202735A5 DD 82240391 A DD82240391 A DD 82240391A DD 24039182 A DD24039182 A DD 24039182A DD 202735 A5 DD202735 A5 DD 202735A5
- Authority
- DD
- German Democratic Republic
- Prior art keywords
- cells
- dna
- galactosidase
- raffinose
- transformable
- Prior art date
Links
- 108010030291 alpha-Galactosidase Proteins 0.000 title abstract description 11
- 238000004519 manufacturing process Methods 0.000 title abstract description 9
- 102000005840 alpha-Galactosidase Human genes 0.000 title abstract description 6
- 239000013612 plasmid Substances 0.000 claims abstract description 48
- 238000000034 method Methods 0.000 claims abstract description 32
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 31
- 239000013598 vector Substances 0.000 claims abstract description 29
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- 230000009466 transformation Effects 0.000 claims abstract description 18
- 230000003115 biocidal effect Effects 0.000 claims abstract description 13
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- 239000006166 lysate Substances 0.000 claims abstract description 6
- 239000000284 extract Substances 0.000 claims description 8
- 108010093031 Galactosidases Proteins 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 7
- 241000589776 Pseudomonas putida Species 0.000 claims description 6
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- 101000588924 Anthopleura elegantissima Delta-actitoxin-Ael1a Proteins 0.000 claims 2
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- MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 abstract description 43
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 abstract description 43
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- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 5
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- 241000894006 Bacteria Species 0.000 description 3
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- 239000004098 Tetracycline Substances 0.000 description 3
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- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
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- CZMRCDWAGMRECN-UHFFFAOYSA-N Rohrzucker Natural products OCC1OC(CO)(OC2OC(CO)C(O)C(O)C2O)C(O)C1O CZMRCDWAGMRECN-UHFFFAOYSA-N 0.000 description 1
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- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 1
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- VHJLVAABSRFDPM-ZXZARUISSA-N dithioerythritol Chemical compound SC[C@H](O)[C@H](O)CS VHJLVAABSRFDPM-ZXZARUISSA-N 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
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- 150000008195 galaktosides Chemical class 0.000 description 1
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- 239000011777 magnesium Substances 0.000 description 1
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- 229910001437 manganese ion Inorganic materials 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
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- PGSADBUBUOPOJS-UHFFFAOYSA-N neutral red Chemical compound Cl.C1=C(C)C(N)=CC2=NC3=CC(N(C)C)=CC=C3N=C21 PGSADBUBUOPOJS-UHFFFAOYSA-N 0.000 description 1
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- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- UQZIYBXSHAGNOE-XNSRJBNMSA-N stachyose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO[C@@H]3[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O3)O)O2)O)O1 UQZIYBXSHAGNOE-XNSRJBNMSA-N 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
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- -1 sulfhydryl compound Chemical class 0.000 description 1
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- 235000019157 thiamine Nutrition 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/848—Escherichia
- Y10S435/849—Escherichia coli
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19813122216 DE3122216A1 (de) | 1981-06-04 | 1981-06-04 | Verfahren zur herstellung eines mikroorganismus, welcher (alpha)-galactosidase, aber keine invertase bildet, so erhaltener mikroorganismus und seine verwendung |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DD202735A5 true DD202735A5 (de) | 1983-09-28 |
Family
ID=6133914
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DD82240391A DD202735A5 (de) | 1981-06-04 | 1982-06-02 | Verfahren zur herstellung invertasefreier alpha-galactosidase |
| DD82255231A DD210467A5 (de) | 1981-06-04 | 1982-06-02 | Verfahren zur spaltung von alpha-galactosidischen zuckern |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DD82255231A DD210467A5 (de) | 1981-06-04 | 1982-06-02 | Verfahren zur spaltung von alpha-galactosidischen zuckern |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US5061625A (cs) |
| EP (1) | EP0066857B1 (cs) |
| JP (1) | JPS57208985A (cs) |
| AT (1) | ATE29525T1 (cs) |
| AU (1) | AU531972B2 (cs) |
| CA (1) | CA1194433A (cs) |
| CS (1) | CS272753B2 (cs) |
| DD (2) | DD202735A5 (cs) |
| DE (2) | DE3122216A1 (cs) |
| DK (1) | DK251682A (cs) |
| HU (1) | HU193266B (cs) |
| SU (1) | SU1431681A3 (cs) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IE81141B1 (en) * | 1983-06-24 | 2000-04-05 | Genencor Int | Procaryotic carbonyl hydrolases |
| SU1364343A1 (ru) * | 1984-07-13 | 1988-01-07 | Всесоюзный научно-исследовательский институт генетики и селекции промышленных микроорганизмов | Способ получени человеческого лейкоцитарного интерферона альфа-2 |
| US5741672A (en) * | 1984-07-27 | 1998-04-21 | Unilever Patent Holdings B.V. | Expression and production of polypeptides using the promoters of the hansenula polymorpha MOX and DAS genes |
| US5240838A (en) * | 1984-07-27 | 1993-08-31 | Internationale Otrool Maatschappij "Octropa" B.V. | Regulatory sequences of alcohol oxidase (MOX) and dihydroxyacetonesynthase (DAS) of Hansenula polymorpha |
| FI861548A0 (fi) * | 1986-04-11 | 1986-04-11 | Alko Ab Oy | Nya jaeststammar vari med gentekniska foerfaranden infoerts en -galaktosidasgen och foerfaranden foer industriellt utnyttjande av dylika jaeststammar. |
| CA1339101C (en) * | 1986-06-03 | 1997-07-29 | Nicolaas Overbeeke | Production of guar alpha-galactosidase and immunologically related alpha-galactosidases by host organisms transformed with recombinant dna methods |
| EP0255153B1 (en) * | 1986-06-03 | 1995-01-18 | Unilever N.V. | Production of guar alpha-galactosidase by hosts transformed by recombinant DNA methods |
| EP0487159A1 (en) * | 1990-11-23 | 1992-05-27 | Unilever N.V. | A food-grade vector suitable for transforming a food-grade host cell, use of said vector for transforming food-grade host cells, and use of said transformed cells in biotransformation processes |
| US6770468B1 (en) | 1999-09-14 | 2004-08-03 | Genzyme Glycobiology Research Institute, Inc. | Phosphodiester-α-GlcNAcase of the lysosomal targeting pathway |
| US6642038B1 (en) | 1999-09-14 | 2003-11-04 | Genzyme Glycobiology Research Institute, Inc. | GlcNAc phosphotransferase of the lysosomal targeting pathway |
| US6800472B2 (en) | 2001-12-21 | 2004-10-05 | Genzyme Glycobiology Research Institute, Inc. | Expression of lysosomal hydrolase in cells expressing pro-N-acetylglucosamine-1-phosphodiester α-N-acetyl glucosimanidase |
| RU2504583C1 (ru) * | 2012-10-03 | 2014-01-20 | Федеральное государственное бюджетное учреждение науки Тихоокеанский институт биоорганической химии им. Г.Б. Елякова Дальневосточного отделения Российской академии наук (ТИБОХ ДВО РАН) | ПЛАЗМИДА 40Gal, ОПРЕДЕЛЯЮЩАЯ СИНТЕЗ α-ГАЛАКТОЗИДАЗЫ α-PsGal, ШТАММ E.coli Rosetta(DE3)/40Gal - ПРОДУЦЕНТ ХИМЕРНОГО БЕЛКА, ВКЛЮЧАЮЩЕГО АМИНОКИСЛОТНУЮ ПОСЛЕДОВАТЕЛЬНОСТЬ α-PsGal, И СПОСОБ ЕЕ ПОЛУЧЕНИЯ |
| CN115417510B (zh) * | 2022-09-20 | 2023-07-04 | 南京农业大学 | 一种水中胞外抗生素抗性基因的去除方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3957578A (en) * | 1975-01-03 | 1976-05-18 | Hokkaido Sugar Co., Ltd. | Method for manufacture of α-galactosidase by microorganism |
-
1981
- 1981-06-04 DE DE19813122216 patent/DE3122216A1/de not_active Withdrawn
-
1982
- 1982-05-18 CS CS363582A patent/CS272753B2/cs unknown
- 1982-05-19 AU AU83826/82A patent/AU531972B2/en not_active Ceased
- 1982-05-27 CA CA000403872A patent/CA1194433A/en not_active Expired
- 1982-06-02 DD DD82240391A patent/DD202735A5/de not_active IP Right Cessation
- 1982-06-02 JP JP57093239A patent/JPS57208985A/ja active Granted
- 1982-06-02 DD DD82255231A patent/DD210467A5/de not_active IP Right Cessation
- 1982-06-03 EP EP82104893A patent/EP0066857B1/de not_active Expired
- 1982-06-03 AT AT82104893T patent/ATE29525T1/de not_active IP Right Cessation
- 1982-06-03 DE DE8282104893T patent/DE3277213D1/de not_active Expired
- 1982-06-03 HU HU821796A patent/HU193266B/hu not_active IP Right Cessation
- 1982-06-04 DK DK251682A patent/DK251682A/da not_active Application Discontinuation
- 1982-06-04 SU SU823448724A patent/SU1431681A3/ru active
-
1988
- 1988-08-24 US US07/235,910 patent/US5061625A/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CS363582A2 (en) | 1990-06-13 |
| CS272753B2 (en) | 1991-02-12 |
| ATE29525T1 (de) | 1987-09-15 |
| EP0066857A2 (de) | 1982-12-15 |
| DK251682A (da) | 1982-12-05 |
| CA1194433A (en) | 1985-10-01 |
| AU531972B2 (en) | 1983-09-15 |
| SU1431681A3 (ru) | 1988-10-15 |
| AU8382682A (en) | 1982-12-09 |
| JPS6251588B2 (cs) | 1987-10-30 |
| EP0066857B1 (de) | 1987-09-09 |
| DD210467A5 (de) | 1984-06-13 |
| JPS57208985A (en) | 1982-12-22 |
| HU193266B (en) | 1987-09-28 |
| US5061625A (en) | 1991-10-29 |
| DE3277213D1 (en) | 1987-10-15 |
| EP0066857A3 (en) | 1983-06-22 |
| DE3122216A1 (de) | 1982-12-23 |
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| ENJ | Ceased due to non-payment of renewal fee |