CY1123581T1 - Νεες μεθοδοι για την παραγωγη ολιγονουκλεοτιδιων - Google Patents

Νεες μεθοδοι για την παραγωγη ολιγονουκλεοτιδιων

Info

Publication number
CY1123581T1
CY1123581T1 CY20201101067T CY201101067T CY1123581T1 CY 1123581 T1 CY1123581 T1 CY 1123581T1 CY 20201101067 T CY20201101067 T CY 20201101067T CY 201101067 T CY201101067 T CY 201101067T CY 1123581 T1 CY1123581 T1 CY 1123581T1
Authority
CY
Cyprus
Prior art keywords
production
oligonucleotides
new methods
therapy
disclosed
Prior art date
Application number
CY20201101067T
Other languages
English (en)
Inventor
Andreas Crameri
Malcolm Leithhead HILL
Sarah Louise LOVELOCK
Markus Schober
David Graham Tew
Pamela Joan Thomas
Original Assignee
Glaxosmithkline Intellectual Property Development Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=56890843&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CY1123581(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Glaxosmithkline Intellectual Property Development Limited filed Critical Glaxosmithkline Intellectual Property Development Limited
Publication of CY1123581T1 publication Critical patent/CY1123581T1/el

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    • C—CHEMISTRY; METALLURGY
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y605/00—Ligases forming phosphoric ester bonds (6.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y605/00—Ligases forming phosphoric ester bonds (6.5)
    • C12Y605/01—Ligases forming phosphoric ester bonds (6.5) forming phosphoric ester bonds (6.5.1)
    • C12Y605/01001—DNA ligase (ATP) (6.5.1.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/315—Phosphorothioates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/34—Spatial arrangement of the modifications
    • C12N2310/341—Gapmers, i.e. of the type ===---===
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/352—Nature of the modification linked to the nucleic acid via a carbon atom
    • C12N2310/3525—MOE, methoxyethoxy
    • C—CHEMISTRY; METALLURGY
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Στο παρόν αποκαλύπτονται νέες μέθοδοι για την παραγωγή ολιγονουκλεοτιδίων, οι οποίες είναι κατάλληλες για χρήση στην παραγωγή χημικώς τροποποιημένων ολιγονουκλεοτιδίων, όπως αυτών για χρήση σε θεραπεία.
CY20201101067T 2016-07-11 2020-11-11 Νεες μεθοδοι για την παραγωγη ολιγονουκλεοτιδιων CY1123581T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1612011.5A GB201612011D0 (en) 2016-07-11 2016-07-11 Novel processes for the production of oligonucleotides
PCT/EP2017/067049 WO2018011067A2 (en) 2016-07-11 2017-07-07 Novel processes for the production of oligonucleotides

Publications (1)

Publication Number Publication Date
CY1123581T1 true CY1123581T1 (el) 2022-03-24

Family

ID=56890843

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20201101067T CY1123581T1 (el) 2016-07-11 2020-11-11 Νεες μεθοδοι για την παραγωγη ολιγονουκλεοτιδιων

Country Status (19)

Country Link
US (2) US10640812B2 (el)
EP (2) EP3481838B1 (el)
JP (3) JP6752957B2 (el)
KR (3) KR102492515B1 (el)
CN (2) CN115161366A (el)
AU (1) AU2017295602B2 (el)
CA (1) CA3030079C (el)
CY (1) CY1123581T1 (el)
DK (1) DK3481838T3 (el)
ES (1) ES2831419T3 (el)
GB (2) GB201612011D0 (el)
HR (1) HRP20201744T1 (el)
HU (1) HUE051767T2 (el)
LT (1) LT3481838T (el)
PT (1) PT3481838T (el)
RS (1) RS61262B1 (el)
RU (1) RU2719641C1 (el)
SI (1) SI3481838T1 (el)
WO (1) WO2018011067A2 (el)

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GB201612011D0 (en) * 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
CA3062550A1 (en) * 2017-05-08 2018-11-15 Codexis, Inc. Engineered ligase variants
RU2020103727A (ru) 2017-06-30 2021-07-30 Кодексис, Инк. Варианты рнк-полимеразы т7
EP3645711A4 (en) 2017-06-30 2021-04-21 Codexis, Inc. T7 RNA POLYMERASE VARIANTS
GB201721307D0 (en) * 2017-12-19 2018-01-31 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
WO2019189722A1 (ja) 2018-03-30 2019-10-03 東レ株式会社 ヘアピン型一本鎖ｒｎａ分子の製造方法
CN111971396A (zh) * 2018-03-30 2020-11-20 住友化学株式会社 单链rna的制造方法
JP7537422B2 (ja) 2019-02-18 2024-08-21 味の素株式会社 相補部分を含む修飾オリゴヌクレオチドの製造方法
KR20210128410A (ko) * 2019-02-20 2021-10-26 로슈 이노베이션 센터 코펜하겐 에이/에스 포스포노아세트산 갭머 올리고뉴클레오티드
US11725073B2 (en) 2020-12-29 2023-08-15 Hongene Biotech Corporation Compositions and methods for liquid phase oligonucleotide synthesis
EP4319792A1 (en) * 2021-04-05 2024-02-14 10X Genomics, Inc. Recombinant ligase composition and uses thereof
US11851454B2 (en) 2021-12-30 2023-12-26 Hongene Biotech Corporation Compositions and methods for liquid phase oligonucleotide synthesis
US11788082B2 (en) * 2022-02-22 2023-10-17 Abclonal Science, Inc. T4 DNA ligase variants with increased thermostability
US20230295707A1 (en) * 2022-03-21 2023-09-21 Abclonal Science, Inc. T4 DNA Ligase Variants with Increased Ligation Efficiency
WO2023194537A1 (en) 2022-04-08 2023-10-12 Glaxosmithkline Intellectual Property Development Limited Novel processes for the production of polynucleotides including oligonucleotides

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Also Published As

Publication number Publication date
EP3702360A1 (en) 2020-09-02
AU2017295602A1 (en) 2018-12-20
GB2558335A (en) 2018-07-11
JP2019525917A (ja) 2019-09-12
HRP20201744T1 (hr) 2020-12-25
GB2558335B (en) 2019-12-04
ES2831419T3 (es) 2021-06-08
HUE051767T2 (hu) 2021-03-29
JP7531537B2 (ja) 2024-08-09
US20180023122A1 (en) 2018-01-25
KR20220013473A (ko) 2022-02-04
CN109415399A (zh) 2019-03-01
SI3481838T1 (sl) 2020-12-31
JP2022104943A (ja) 2022-07-12
JP2020188812A (ja) 2020-11-26
CA3030079C (en) 2021-12-07
KR102205974B1 (ko) 2021-01-22
AU2017295602B2 (en) 2019-07-11
KR20210008179A (ko) 2021-01-20
DK3481838T3 (da) 2020-11-16
LT3481838T (lt) 2020-12-10
GB201710919D0 (en) 2017-08-23
EP3481838A2 (en) 2019-05-15
WO2018011067A2 (en) 2018-01-18
CN109415399B (zh) 2022-05-24
WO2018011067A3 (en) 2018-02-22
RS61262B1 (sr) 2021-01-29
GB201612011D0 (en) 2016-08-24
KR20190022726A (ko) 2019-03-06
PT3481838T (pt) 2020-11-20
KR102356524B1 (ko) 2022-02-08
US10640812B2 (en) 2020-05-05
US20200277659A1 (en) 2020-09-03
RU2719641C1 (ru) 2020-04-21
EP3481838B1 (en) 2020-08-19
CN115161366A (zh) 2022-10-11
KR102492515B1 (ko) 2023-01-27
BR112018076725A2 (pt) 2019-04-02
JP6752957B2 (ja) 2020-09-09
CA3030079A1 (en) 2018-01-18
JP7050866B2 (ja) 2022-04-08

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