CU22968A1 - Procedimiento para la detección de anticuerpos anti transglutaminasa con utilidad en el diagnóstico de la enfermedad celíaca - Google Patents

Procedimiento para la detección de anticuerpos anti transglutaminasa con utilidad en el diagnóstico de la enfermedad celíaca

Info

Publication number
CU22968A1
CU22968A1 CU20000132A CU20000132A CU22968A1 CU 22968 A1 CU22968 A1 CU 22968A1 CU 20000132 A CU20000132 A CU 20000132A CU 20000132 A CU20000132 A CU 20000132A CU 22968 A1 CU22968 A1 CU 22968A1
Authority
CU
Cuba
Prior art keywords
transglutaminase
diagnosis
detection
celiac disease
deposited
Prior art date
Application number
CU20000132A
Other languages
English (en)
Inventor
Castro Boris Ernesto Is Acevedo
Gomez Luis Tomas Sorell
Original Assignee
Ct Ingenieria Genetica Biotech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ct Ingenieria Genetica Biotech filed Critical Ct Ingenieria Genetica Biotech
Priority to CU20000132A priority Critical patent/CU22968A1/es
Priority to US09/866,232 priority patent/US6905835B2/en
Priority to CA002349274A priority patent/CA2349274C/en
Priority to ARP010102663A priority patent/AR028676A1/es
Priority to BR0102281-4A priority patent/BR0102281A/pt
Priority to ES01202169T priority patent/ES2267662T3/es
Priority to SI200130636T priority patent/SI1164375T1/sl
Priority to PT01202169T priority patent/PT1164375E/pt
Priority to RU2001115777/14A priority patent/RU2214835C2/ru
Priority to EP01202169A priority patent/EP1164375B1/en
Priority to AT01202169T priority patent/ATE335203T1/de
Priority to MXPA01005698A priority patent/MXPA01005698A/es
Priority to DK01202169T priority patent/DK1164375T3/da
Publication of CU22968A1 publication Critical patent/CU22968A1/es
Priority to CY20061101547T priority patent/CY1105739T1/el

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/564—Immunoassay; Biospecific binding assay; Materials therefor for pre-existing immune complex or autoimmune disease, i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/97—Test strip or test slide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/971—Capture of complex after antigen-antibody reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/81—Tube, bottle, or dipstick

Abstract

Método no instrumental para el diagnóstico de la enfermedad celíaca, basado en los principios generales de los ensayos inmunocromatográficos que permite, de forma sencilla, rápida y confiable la detección de anticuerpos anti transglutaminasa, tanto de clase IgA como IgG, en muestras de suero, plasma o sangre, a través de su reacción con una sonda del antígeno transglutaminasa marcado por una sustancia coloreada como oror coloidal o partículas de latex coloreado, que se encuentra depositado sobre una matriz de la que es liberado al ponerse en contactpo con muestras líquidas. Este inmunocomplejo migra a través de un soporte sólido, como nitrocelulosa o nylon, el cual posee un poro que permite el flujo lateral de las sustancias reaccionantes, hasata que es atrapado al interactuar con el propio antígeno transglutaminasa, que se encuentra firmemente unido a una zona reactiva del soporte. Debido a esta unión el inmunocomplejo se deposita en el sitio de reacción lo que se evidencia por la aparición de una marca visualmente detectable en ese sitio, debido al color del marcador que forma parte del complejo. La aparición de esta marca indica un resultado positivo de la prueba a la presencia de anticuerpos anti transglutaminasa en la muestra analizada.
CU20000132A 2000-06-07 2000-06-07 Procedimiento para la detección de anticuerpos anti transglutaminasa con utilidad en el diagnóstico de la enfermedad celíaca CU22968A1 (es)

Priority Applications (14)

Application Number Priority Date Filing Date Title
CU20000132A CU22968A1 (es) 2000-06-07 2000-06-07 Procedimiento para la detección de anticuerpos anti transglutaminasa con utilidad en el diagnóstico de la enfermedad celíaca
US09/866,232 US6905835B2 (en) 2000-06-07 2001-05-25 Assay for anti transglutaminase antibodies detection useful in celicac disease diagnosis
CA002349274A CA2349274C (en) 2000-06-07 2001-05-31 Assay for anti transglutaminase antibodies
ARP010102663A AR028676A1 (es) 2000-06-07 2001-06-04 PROCEDIMIENTO PARA LA DETECCION DE ANTICUERPOS ANTITRANSGLUTAMINASA CON UTILIDAD EN EL DIAGNoSTICO DE LA ENFERMEDAD CELíACA
PT01202169T PT1164375E (pt) 2000-06-07 2001-06-06 Ensaio para anticorpos anti-transglutaminase
ES01202169T ES2267662T3 (es) 2000-06-07 2001-06-06 Ensayo de deteccion de anticuerpos anti-transglutaminasa.
SI200130636T SI1164375T1 (sl) 2000-06-07 2001-06-06 Preizkus za anti-transglutaminazna protitelesa
BR0102281-4A BR0102281A (pt) 2000-06-07 2001-06-06 Ensaios para detecção de anticorpos anti transglutaminase úteis no diagnóstico de doença celìaca
RU2001115777/14A RU2214835C2 (ru) 2000-06-07 2001-06-06 Метод обнаружения антител к трансглутаминазе для применения в диагностике целиакии
EP01202169A EP1164375B1 (en) 2000-06-07 2001-06-06 Assay for anti transglutaminase antibodies
AT01202169T ATE335203T1 (de) 2000-06-07 2001-06-06 Test für anti-transglutaminase-antikörper
MXPA01005698A MXPA01005698A (es) 2000-06-07 2001-06-06 Procedimiento para la deteccion de antcuerpos anti transglutaminasa con utilidad en el diagnostico de la enfermedad celiaca.
DK01202169T DK1164375T3 (da) 2000-06-07 2001-06-06 Assay til antitransglutaminaseantistoffer
CY20061101547T CY1105739T1 (el) 2000-06-07 2006-10-26 Δοκιμασια για αντι τρανσγλουταμινασης αντισωματα

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU20000132A CU22968A1 (es) 2000-06-07 2000-06-07 Procedimiento para la detección de anticuerpos anti transglutaminasa con utilidad en el diagnóstico de la enfermedad celíaca

Publications (1)

Publication Number Publication Date
CU22968A1 true CU22968A1 (es) 2004-07-14

Family

ID=40287551

Family Applications (1)

Application Number Title Priority Date Filing Date
CU20000132A CU22968A1 (es) 2000-06-07 2000-06-07 Procedimiento para la detección de anticuerpos anti transglutaminasa con utilidad en el diagnóstico de la enfermedad celíaca

Country Status (14)

Country Link
US (1) US6905835B2 (es)
EP (1) EP1164375B1 (es)
AR (1) AR028676A1 (es)
AT (1) ATE335203T1 (es)
BR (1) BR0102281A (es)
CA (1) CA2349274C (es)
CU (1) CU22968A1 (es)
CY (1) CY1105739T1 (es)
DK (1) DK1164375T3 (es)
ES (1) ES2267662T3 (es)
MX (1) MXPA01005698A (es)
PT (1) PT1164375E (es)
RU (1) RU2214835C2 (es)
SI (1) SI1164375T1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2214108B1 (es) * 2002-08-16 2005-11-16 Consejo Sup. Investig. Cientificas Sistema y procedimiento inmunocromatografico para la identificacion simultanea de anticuerpos frente a aga y t-tg, y su uso en el diagnostico de enfermedad celiaca.
FR2857453B1 (fr) * 2003-07-11 2007-04-20 Covalab Procede de detection serologique de la maladie coeliaque et necessaire de diagnostic associe
WO2006098804A2 (en) 2005-03-11 2006-09-21 Chembio Diagnostic Systems, Inc. Dual path immunoassay device
US7189522B2 (en) 2005-03-11 2007-03-13 Chembio Diagnostic Systems, Inc. Dual path immunoassay device
JP4982107B2 (ja) * 2006-04-28 2012-07-25 アルフレッサファーマ株式会社 免疫学的微小粒子の凝集反応を用いる検体の測定方法および測定用キット
WO2008012650A2 (en) * 2006-07-25 2008-01-31 Augurix S.A. An immunochromatography device for the diagnosis of diseases in a sample
US7935538B2 (en) * 2006-12-15 2011-05-03 Kimberly-Clark Worldwide, Inc. Indicator immobilization on assay devices
US20100022916A1 (en) 2008-07-24 2010-01-28 Javanbakhsh Esfandiari Method and Apparatus for Collecting and Preparing Biological Samples for Testing
US20100290948A1 (en) * 2009-05-15 2010-11-18 Xuedong Song Absorbent articles capable of indicating the presence of urinary tract infections
CA2827669C (en) 2011-01-20 2018-08-07 Immunosciences Lab, Inc. Methods and apparatus for detection of gluten sensitivity, and its differentiation from celiac disease
US8603835B2 (en) 2011-02-10 2013-12-10 Chembio Diagnostic Systems, Inc. Reduced step dual path immunoassay device and method
NL1039468C2 (nl) * 2012-03-15 2013-09-18 Maria Johanna Kousemaker Glutensensitiviteit bij het paard (equine gse).
US9891216B2 (en) 2014-04-02 2018-02-13 Chembio Diagnostic Systems, Inc. Immunoassay methods utilizing trapping conjugate
US20160116466A1 (en) 2014-10-27 2016-04-28 Chembio Diagnostic Systems, Inc. Rapid Screening Assay for Qualitative Detection of Multiple Febrile Illnesses

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4012494A (en) * 1971-12-21 1977-03-15 Abbott Laboratories Direct radioimmunoassay for antigens and their antibodies
CA1303983C (en) * 1987-03-27 1992-06-23 Robert W. Rosenstein Solid phase assay
US5120643A (en) * 1987-07-13 1992-06-09 Abbott Laboratories Process for immunochromatography with colloidal particles
DE19630557C2 (de) * 1996-07-18 1998-07-02 Schuppan Detlef Priv Doz Dr Dr Verfahren zum Nachweis von Antikörpern aus Körperflüssigkeiten durch eine Immunreaktion mit Gewebe-Transglutaminase (tTG) sowie die Verwendung von tTG in Diagnose und Therapie
CA2387359A1 (en) * 1999-10-20 2001-04-26 Immco Diagnostics Immunological assay for detection of antibodies in celiac disease
US6667160B2 (en) * 2000-03-15 2003-12-23 Kenneth D. Fine Method for diagnosing immunologic food sensitivity

Also Published As

Publication number Publication date
CY1105739T1 (el) 2010-12-22
DK1164375T3 (da) 2006-12-04
AR028676A1 (es) 2003-05-21
US20020006633A1 (en) 2002-01-17
EP1164375A2 (en) 2001-12-19
EP1164375A3 (en) 2002-09-25
MXPA01005698A (es) 2003-08-20
EP1164375B1 (en) 2006-08-02
US6905835B2 (en) 2005-06-14
RU2214835C2 (ru) 2003-10-27
CA2349274C (en) 2009-08-25
ES2267662T3 (es) 2007-03-16
CA2349274A1 (en) 2001-12-07
BR0102281A (pt) 2002-02-19
SI1164375T1 (sl) 2006-12-31
PT1164375E (pt) 2006-12-29
ATE335203T1 (de) 2006-08-15

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