CS268787B1 - Laser fluorometric detector for microcolumn chromatography - Google Patents

Laser fluorometric detector for microcolumn chromatography Download PDF

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Publication number
CS268787B1
CS268787B1 CS863008A CS300886A CS268787B1 CS 268787 B1 CS268787 B1 CS 268787B1 CS 863008 A CS863008 A CS 863008A CS 300886 A CS300886 A CS 300886A CS 268787 B1 CS268787 B1 CS 268787B1
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CS
Czechoslovakia
Prior art keywords
laser
flow cell
fluorometric detector
microcolumn chromatography
microcolumn
Prior art date
Application number
CS863008A
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English (en)
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CS300886A1 (en
Inventor
Alexandr F Lobazov
Vasilij A Mostovnikov
Sergej V Necajev
Boris G Belenkij
Original Assignee
Alexandr F Lobazov
Vasilij A Mostovnikov
Sergej V Necajev
Boris G Belenkij
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Alexandr F Lobazov, Vasilij A Mostovnikov, Sergej V Necajev, Boris G Belenkij filed Critical Alexandr F Lobazov
Publication of CS300886A1 publication Critical patent/CS300886A1/cs
Publication of CS268787B1 publication Critical patent/CS268787B1/cs

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6402—Atomic fluorescence; Laser induced fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/85—Investigating moving fluids or granular solids

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optical Measuring Cells (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Silicon Compounds (AREA)

Description

268 787 - 1 -
ΟΠΗΟΑΗΗΕ H3OBPETEHHH K ABTOPCKOMY CBHAETEJItCTBY 3θηβλθηο 12.05.85 3893760/31-25
KfflH4 G 01 N 30/74
Abtoph HsoepeTGHMH: Α.Φ. JIoCasoB, B.A. Moctobhkkob, C.B. HenaeB η Ε.Γ. ΒθλθηβκηΚ
3&πβμτθλι,: JÍHCTiiTyT φκοκκκ ΛΗ BCCP JIA3EPHUÉ ®JiyOPHMETPHHECKHfó ΛΕΤΕΚΤ0Ρ flJIfl ΜΗΚΡΟΚΟΛΟΗΟΗΗΟίϊ ΧΡΟΜΑΤΟΓΡΑΦΗΗ ΗβοΟρβτβΗΚθ othocktch k xasepHoK οπβκτροοκοπΗΗ κ uosteT 6htb κοπολββοβηηο λλη θηθληβηΜΘΛΗΧ ΚΟΛΚΗΘΟΤΒ (JUiyopeCHIipyBmHX ΚΗΑΚΟΟΤβδ, HanpMMep, Β ΜΗΚρΟΚΟΛΟΗΟΗΗΗΧ SCHAKOCTHUX XpOMaTO-rpa$ax.
Hejtb Μ3θ6ρβΤΘΗΗΗ - ΠΟΒΗΠΙβΗΗβ UyBCTBHTeXbHOCTH Η ρθβρβΙΠΘΒΠ,θδ ΟΠΟΟΟβΗΟΟΤΗ ΑβΤβΚΤΟρβ.
Ha φΗΓ.1 M3o0paKeHa οπτηίθοκθη cxeiia npeflxaraeMoro ycTpofiCTBa; Ba Φηγ.2 - κββθτθ ηΑΜθφρβΓΜΘ.
CxeMa OOCTOMT H3 ΟΠΤΗΗΘΟΚΗ ΌΒΗβθΗΗΗΧ Λθθβρθ 1, ΗΗΛΗΗΑΡΗΊβΟΚΟΓΟ ΤβΛβΟΚΟΠΘ 2, peryAHpye-μοΩ ΛΗβφρθΓΜΗ 3, npHMoyrojibHOft ΚΒβρςθΒοϋ κββθτη 4 c BHyTpeHHHU κθηθλομ κπη προτοκβ Hccxeflye-uoK xhakocth, CBeTOJioByuixH 5, npneMHKxa 6 HaxyuenHS tJuiyopacneHpHM.
XpoMaTorpa$nqecKas κολοηκθ εβκρθΠΛθΗβ pesbĎOBHU οοθαηηθηκθμ k BXOAHOMy Topqy κββθτη,nOMémeHHOS BHyTpi ΟΠρθΒΗ-ΑβρΚΘΤβΛΛ. Městky KBBGTOfi H ΚΟΛΟΗΚΟβ yCTaHOBJíeH φΟΒΤρ. Λβτβκτορ paĎoTaei cxeflyxiamu oCpaeou:
JlasepHoe HaxyueHne φορΜΗ^βτσ* χκΛΗΗΑρΗηεοκΗΜ τθλθοκοπομ b BepTnxajiiHyio noxocKy, πικιρκ—
Ha κοτοροϋ paBHa ιπκρκΗβ κθηηλθ Ρ,χχ προτοκβ μοολθ^θμοΗ xhakocth. Srna πολοοκπ xaaepnoroKajiyneHHH peryjiHpyeTCH o nouomhB ακβΦρθγμη, ycTaHOaxeiiHoK πβρβΛ κββθτοΚ Αβτβκτορβ. flaaeejtaeepHoe ii3JiyueHne προχοΑκτ nepas KBBGTy, οτολ6 Hccjieflyenoii kmakocth x racxTcx b οβθτολο-ByniKe, H3xyne«Me φΛyopecμeHHHH coCxpaeTCH ο6βθκτηβομ b πλοοκοοτη, nepneHflHKyxHpnoii BosCyx-ΑΘΗΗΒ.
PeryjwpyeMaa αηθΦρθγμθ b φορκβ npsuoyroxbHoft Tpanenxx 3γοτθηοβλθηθ TaxHM'.oCpa8OU, uto eerpaHb, nepneHAHKyjixpHaa οοηοβηηκβ, pacnojtoxena πθρθλλθλβηο οτθηκθ κββθτη. flaHHoe pacnoxoxe-Hite αηθΦρθγμη oCecnenHBaeT BosCyxAeHHe φΛyopθe^eH^HK b οτολ6θ HccxeflyeMoK jkhakocth cpasy senocxe ee bhxoas na xpouaTorpa^xnecKofi κολομκη, τθμ cauuu cboaht x UHHHMyuy ποολθκολοηοηηηΚo6i>eu κηακοοτηογο τρβκτθ χβτβκτορθ. nepeuemeHHe αηθΦρθγμη b γορη3Οητβλι»ηοΚ πλοοκοοτη ποθβο-ΛΗβΤ Β ΟΠρθΑΘΛΘΗΗΗΧ ΠρθΑΘΛΘΧ ΠΛΘΒΗΟ ΜΘΗΗΤΒ BHCOTy ΟΤΟΛδθ KHAKOCTK, BO80yMAaeMoK ΛθβθρΗΗΜMBtfyHeHMeM. npeuMymecTBOM npe^araeiíofi KOHCTpyKUMH no ορθβηθηηβ c ΗβΒβοτιιοϋ λβληθτοη ποβηπιθηηθ wyB-ΟΤΒΗΤβΛΒΗΟΟΤΗ HH ΑΒΘ nOpHflKa Η ρββρβΠΙΘΒΙΙΙθδ ΟΠΟΟΟΟΗΟΟΤΗ Β 1,5-2 ρβθβ. ΠρκΜβρ.
MsMepHTexbHas κββθτθ Αθτβκτορβ ηβγοτοβλθηθ hs KBapqa, κοτορυδ ne XBMHxecAHpyeT πόα Αβδοτ-
Bueii Λ38βρκθΓο H3AyneHHH. Hapyraue pasMepu κββθτη 4x10x20 mu, BHyTpeHHHfi κθηηλ 0,5x0,5 um.
ΙξΗΛΗΗΑρκηβΟΚΜδ ΤβΛβΟΚΟΠ ΟΟθΟΠΘΗΗΒΗθΤ φορΜΙίρΟΒΘΗΗβ ΛθββρΗΟΓΟ HSXyneHHH Β ΒΗΑβ ΠΟΛΟΟΚΗ paSUepOU 0,5x7 mu. Αλπ cosAaHHH οτροΓο npxMoyroxbBoň φορΜΒ xasepHoro HSjyneHHJt ο paBHOuepHHM pacnpexe- ΛβΠΗβΜ MOIQHOCTH Π0 ΠΛΟΑΘΑΗ, ΑΗθφραΓΜΘ ΟΓρθΗΗΗΗΒΘβΤ UaKCHMaAbHyB A^KHy ΠΟΛΟΟΚΗ Α° 6 MM, UHHH- ΜΘΛΒΗβΗ ΑΛΠΗβ ΠΟΛΟΟΚΗ 1 MU. ΤβΚΗΜ OÓpaSOM, ASTeKTIipy ΘΜΗβ θ6ΐ>ΘΜ ΜΟΚΘΤ ΠΛΟΒΗΟ peryAHpOBHTbCa οτ 0,25 flo 1,5 μκλ. ΠοολθκολοηοηημΚ ο6·βθμ Αβτβκτορβ ηθ 6ολθθ 0,05 μκλ. MexyneHne (fiAyopeoueH- ΗΗΗ BoedyxcflaeTca αληηοΠ βοληη 325 bu η perHOTpHpyeTOH ηθ αληηθ βοληη 550 su. Οηοτθμθ perx- CTpanHH paCoTaeT β ρθκημθ οηβτβ οαηοθλθκτροηηηχ KMnyxbcoB. Πρπ 3THX yCAOBHHX Η ΜΗΚρΟΚΟΛΟΗΚΟδ 0,6x170 UU (paSpeoiaBUiaH CnOCOĎKOCTb 8*10S ΤΘΟρθΤΗΊΘΟΚΗΧ*·τβ τβρβΛοκ) noAyneHa noporoBaa ^βοτβητθλβηοοτβ αθτθκτηροβθηηη ΚΗΟ-θμηηοκηολοτ 2ΊΟ μολβ βΧρΟΜΟΤΟΓρθφΗΗβΟΚΟΜ ΠΗΚβ. - 2 - 268 787 ΦΟΡΜΖΠΑ Μ30ΒΡΕΓΕΗΜΗ
JlaaepHun (JkiyopjjueTpoecKHfi Λβτβκτορ λλη mhkpokoxohouhoB xpoMaTorpaíwn, cojiepwanHÍi on-TwuecKH CBasamoie «aaep, yaea λλη φορΜκροΒβΗΗΗ ΛθββρΗΟΓΟ MaJtynemia, npoTOWíiyjo KBBeTy, οβθτο-jioBymKy, flna$parMy μ πρκβΜΗΒΚ ua^yveiiua (JwtyopecneHUiiH, οτλη^θβ^ηΚοη τθμ, hto,c qexbB ποβηιπθημη ^βοτβητθλβηοοτμ h paspewaBqeíi cnocoCnocTn Αβτθκτορβ, yaex fopMHpoBQHHHJiasepnoro H3xytie!!MH βηποληθη b bhso ηκΛίΐΗΛρκΊβοκοΓΟ τβΛβοκοπβ, a iteac^y θτκμ TejtecKonou κπροτοΊΗοΚ κββθτοΗ pacnojioaceHa ΛκβφρθΓίΐβ, ΒΗποΛΗβΗΗΟΗ β BMfle npHMoyro^bHoK TpaneqaK Κ yCTa-ΗΟΒΛβΗΗβΗ C BO3MOKHOCTbB ΠβρβΜβΙΙξθΗΗΗ S IMOCKOCTH, ΠβρΠΘΗΛΗ^ΛίίρΗΟδ ΗΘΠρβΒΛΘΗΗΒ Λ03θρΜΟΓΟMaxyHeuMB πρμπθμ rpam>, nepneHqHKyjiHpHOH ochobghiib TpaneqHM, pacnojtoKena napaoe,n>HO οτβΗκβπροτοπκοδ ΚΒΒβΟΉ. - 3 - 268 787
Ρ Ε Φ E P A T JIA3EPHUÍÍ ΦΛΥΟΡΜΜΕΤΡΜηΕΟΚΜβ ÍETEKTOP jyifl MKlíPOKOJIOHCTCHOft ΧΡΟΜΑΤΟΓΡΑΦΜΗ
HaodpeTeHMe othocktch k aasepnoíi οπεκτροοκοπΜΗ κ μοκθτ 6ητβ ηοπολββοβθηο β JiasepHuxφΛγΟρΚΜΘΤρθΧ RJIX ΜΚΚρΟΚΟΛΟΗΟΗΗΟϋ ΧρΟΜΘΤΌΓρθφΗΗ. HeJIbB Η3θ0ρθΤβΗ3 ΗΒΛΗΘΤΟΒ ΠΟΒΙΠΠΘΜΜΘ MyB-CTBHTS4bHOCTM Η ρθθρθΙΠΘΒΓΙΙθβ CHOCOCHOCTH ΧβΤβΚΤΟρβ. Β ΛθδθρΗΟΜ φ^ΟρΚΜβτρίΟΙβΟΚΟΙΙ ΛβΤΘΚΤΟρβΟΗΟΤΘΜΟ ŤOpMMpOBaHHH ΛβθθρΗΟΓΟ M3JiyMGHMH ΒΙΓΠΟΛΗΘΗβ Β ΒΜΑΘ ΡΜΛΗΗΛΡΚ'ΙΘΟΚΟΓΟ ΤΘΛΘΟΚΟΠΘ 2 ΚTpaneuneBHAHOří Aiia$panui 3. HiwiMHApimecKMÍí τθαθοκοπ Z npeBpaiqaai najiytienMe jcasepa β προβκ-Amm b npaMoyroAbHyn noAOCKy, cooTBeTCTBynqy» no φορωβ KSHajiy c ncejieflyeMoa sekakoctb» β προtomhoIí κκβθτθ 4./(Ha$parMa 3 peryAHpyeT AJiMHy npauoyroabHotl ποαοοκη οοοτΒβτοτΒβππο βθληίιιηθχροΜοτοΓρβφιιηβοκοΓΟ πμκθ. Φμγ.1, φκΓ.2.

Claims (1)

  1. PŘEDMĚT VYNÁLEZU
    Laserový fluorimetrický detektor pro mikrosloupcovou chromatografií, obsahující opticky spojené laser, uzel pro tvarování laserového záření, průtokovou kyvetu, světelný lapač, clonu a přijímač záření fluorescence, vyznačující se tím, že uzel tvarování laserového záření je proveden ve tvaru válcovitého teleskopu (2), a mezi tímtb teleskopem (2) a průtokovou kyvetou (4) je umístěna clona (3) ve tvaru pravoúhlého lichoběžníku a umístěna s možností posunu v rovině, kolmé ke směru laserového záření, přičemž plocha kolmá к základně lichoběžníku je umístěna paralelně ke stěně průtokové kyvety.
CS863008A 1985-05-12 1986-04-25 Laser fluorometric detector for microcolumn chromatography CS268787B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU853893760A SU1376042A1 (ru) 1985-05-12 1985-05-12 Лазерный флуориметрический детектор дл микроколоночной хроматографии

Publications (2)

Publication Number Publication Date
CS300886A1 CS300886A1 (en) 1989-06-13
CS268787B1 true CS268787B1 (en) 1990-04-11

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ID=21176583

Family Applications (1)

Application Number Title Priority Date Filing Date
CS863008A CS268787B1 (en) 1985-05-12 1986-04-25 Laser fluorometric detector for microcolumn chromatography

Country Status (10)

Country Link
US (1) US4822167A (cs)
AT (1) AT390841B (cs)
CS (1) CS268787B1 (cs)
DD (1) DD270643A3 (cs)
DE (1) DE3627448A1 (cs)
FR (1) FR2603106B1 (cs)
GB (1) GB2193571B (cs)
HU (1) HU201155B (cs)
SE (1) SE458156B (cs)
SU (1) SU1376042A1 (cs)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6830731B1 (en) * 1998-01-05 2004-12-14 Biosite, Inc. Immunoassay fluorometer
US6195214B1 (en) * 1999-07-30 2001-02-27 Etec Systems, Inc. Microcolumn assembly using laser spot welding

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4031399A (en) * 1975-02-24 1977-06-21 Beckman Instruments, Inc. Fluorometer
US3999861A (en) * 1975-06-30 1976-12-28 Technicon Instruments Corporation Flow cell
FR2348486A1 (fr) * 1976-04-15 1977-11-10 Commissariat Energie Atomique Procede et dispositif d'analyse d'echantillon par spectrographie d'emission utilisant un faisceau laser
US4203670A (en) * 1977-04-21 1980-05-20 Bromberg Nathan S System and method of fluorescence polarimetry
US4284355A (en) * 1979-10-29 1981-08-18 Ortho Diagnostics, Inc. Automated method for cell volume determination
US4350892A (en) * 1980-07-31 1982-09-21 Research Corporation X'-, Y'-, Z'- axis multidimensional slit-scan flow system
DD159566A1 (de) * 1981-06-10 1983-03-16 Hartmut Lucht Spektralfluorometer
DE3208919A1 (de) * 1982-03-12 1983-09-22 Gesellschaft für Strahlen- und Umweltforschung mbH, 8000 München Anordnung zur messung der fluoreszenzpolarisation
US4537861A (en) * 1983-02-03 1985-08-27 Elings Virgil B Apparatus and method for homogeneous immunoassay
US4555177A (en) * 1983-12-22 1985-11-26 The United States Of America As Represented By The Secretary Of The Navy Method and apparatus for detecting singlet state resonance fluorescence
US4643566A (en) * 1984-07-20 1987-02-17 Canon Kabushiki Kaisha Particle analyzing apparatus
SE455646B (sv) * 1984-10-22 1988-07-25 Radians Innova Ab Fluorescensanordning

Also Published As

Publication number Publication date
GB2193571B (en) 1990-07-04
SE8603622D0 (sv) 1986-08-27
CS300886A1 (en) 1989-06-13
GB8619282D0 (en) 1986-09-17
AT390841B (de) 1990-07-10
SE458156B (sv) 1989-02-27
FR2603106A1 (fr) 1988-02-26
HUT44652A (en) 1988-03-28
DE3627448C2 (cs) 1990-02-22
GB2193571A (en) 1988-02-10
DE3627448A1 (de) 1988-02-18
SE8603622L (sv) 1988-02-28
ATA209286A (de) 1989-12-15
SU1376042A1 (ru) 1988-02-23
FR2603106B1 (fr) 1988-12-09
DD270643A3 (de) 1989-08-09
HU201155B (en) 1990-09-28
US4822167A (en) 1989-04-18

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