CS245736B1 - Apparatus for continual desintegration of microorganism cells - Google Patents

Apparatus for continual desintegration of microorganism cells Download PDF

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Publication number
CS245736B1
CS245736B1 CS823379A CS337982A CS245736B1 CS 245736 B1 CS245736 B1 CS 245736B1 CS 823379 A CS823379 A CS 823379A CS 337982 A CS337982 A CS 337982A CS 245736 B1 CS245736 B1 CS 245736B1
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CS
Czechoslovakia
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sleeve
cycneh3hh
vessel
kopnyc
needle
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CS823379A
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English (en)
Inventor
Alfred N Grigorjan
Andrej P Kovalev
Vitalij D Lalov
Nikolaj D Makarov
Radij V Katrus
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Alfred N Grigorjan
Andrej P Kovalev
Vitalij D Lalov
Nikolaj D Makarov
Radij V Katrus
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Publication of CS245736B1 publication Critical patent/CS245736B1/cs

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Disintegrating Or Milling (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)

Description

-2- Ií3oópereHHe othochtch k MHKpotíHOjiormecKoS npoMUm-λθηηοοτη h npejtHa3Ha^eH0 jyifl pa3pymeHo κλθτοκ MHKpoopra-HH3M0B, ΤβΚΗΧ,ΚβΚ ΛΡ080ΚΗ, óaKTepHH, BOflOpOCJUi, rpHÓH, ΛΛΗ ΒΗΛθΛΘΗΗΗ BHyTpHKXeTOHHUX 0ΘΛΚΟΒ, HyKJieHHOBHX KHC—ΛΟΤ, φβρΜΘΗΤΟΒ H flpyrHX φΗ3ΗΟΛΟΓΗΗΘΟΚΗ aKTHBHHX ΒΘ^ΘΟΤΒ.
HsBecTHH ycTaHOBKH λλη j;e3HHTerpaiiHH κλθτοκ Μκκρο-OpraHHSMOB C Η0Π0ΛΒ30Β3ΗΗΘΜ ΘΟ.φθΚΤΘ fleKOMnpeCCHH, B KO-Topux pa3pynieHHe κλθτοκ npoHcxoaHT 3a οηθτ pacnizpeHHHra3a, pacraopeHHoro πολ λθβλθηηθμ b κλθτκθχ, npa ρββκοΜcópoce ΛΗΒΛΘΗΗΗ. Η3ΒΘ0ΤΗΗΘ ycTaHOBKH ΛΘΚΟΜΠρβΟΟΗΟΗΗΟΑ βθΒΗΗΤΘΓρβΙίΗΗBKJimaioT £Ba ochobhhx y3Jta, H3 κοτορκχ nepBHfi cojiepraTo#hh on jfBa cocy.ua bhc οκογο λθβλθηηη, oóopyflOBaHHHxTpyÓOnpOBOflaMH λλη πολεοαθ k hhm κλθτο^ηο® cycneH3HH Hcacaioro paóonero raea sjih Hacumeaufl cycneH3HH, h BTopofi- y3eji fl;e3HHTerpanHH, - Koropufí πρθΛΟΤβΒΛΗβτ coóofi oa-naH οπθΐριβΛΒΗοβ KOHCTpyKUHH, oóecneHHBaioinHfi pe3KHflcdpoc λθβλθηηη b ποτοκθ κΛβτοπΗοβ cycneoHB [l,2] . H3BecTHtje ycTaHOBKH jteKOMnpeccHOHHoň ae3HHrerpanHHΚΛΘΤΟΚ MHKp00praHH3M0B pa3XHHa£)TCH ΜΘΗζΟcodoa, ΓΛΗΒΗΗΜotípa3OM. KOHCTpyKHHeĚ y3Jia ^e3HHTerpamiH. B oshom Bapnanre ype^ ,ne3HHrerpamiH πρθαοτηβληθτcodofl KOHcrpyKurao. coctohdctio H3 βθρχηθγο η ηημθγο 245736 -3- $JiaHHeotípa3HHX KOpnyCOB, CMOHTHpOBaHHHX C B03MOJKHOCTHOhx nepeMemeura no ΒβρτΗκαω h CHaóxeHHHx pacnojiOKeHHHMHb hhx TapeaiBnaTHMH cejyiaMH, npmewi Tapejitnaroe cejyioHHJKHero Kopnyca ΗΜββτ KOJimeodpasanfi Bucryn, oópa3ymiiínSc HapyaHoft ποΒβρχΗοβτκο sopxHero cejyia patíonyio mejii., aΒβρχΗΗβ Kopnyc ΗΜββτ KaHaji kjih BBona cycneH3HH b paóony»mejxi, [i], B flpyroM BapnaHTe npHMeHeHa aHajiorannan KOHcrpyK-mM yejia fle3HHTerpamiH, oumnaromanca τβΜ, nro cenjioHHxnero Kopnyca ΗΜββτ ^onojxHHTejiBHuíi KojxtneBofi Bticrynh KaHaji p,Jisi oTBona cycneH3HH paspymeHHux κλθτοκ, a Ta-pejitnaToe cejyio BepxHero Kopnyca ΗΜββτ pnn προτοηβκ,cootíqeHHHx c nonBosamuM cycneH3Hio κβηηλομ η cjiyacamHx,HJM pacnpenejieHun ee b padonyio mejih; κροΜβ τογο, HaΒθρχΗβΜ Kopnyce ycTanoBxeH BCTpHXHBarejiB kjih ohhctkhpatíonefi mejm npn ee 3acopeHHH [ 2 ].
OnHcanHue ycTaHOBKH ηβκοΜπρβββΗΟΗΗοίί ne3HHrerpa-iíhh paÓoTaioT cjienyroinnM oópa3OM. EjieTovny» cycneHsioo hcataruS ra3 HenpepHBHO nonaioT b cocyn-CMecHTeju>, ocHa-meHHHfl ypoBHeMepoM nonaasacEoromna. Πρπ noBumeHHH ypoB-hh cycneH3HH b cocyne-CMecHTejte ypoBHeMep οτκρκΒββτnocTyn b Hero csaTOMy ra3y, κοτορίίβ dapóOTHpyeT něpeecjiofi cycneH3HH non nasjíenneM. flanee cycneH3HH, HacHinen-Han ra30M, προχοηπτ nepee peccimep h nociynaeT Ha yeejine3HHTerpanHH. B yejie ne3HHrerpanHH cycneH3HH nocTynaeT b Tapeju»-naroe cejyio BepxHero Kopnyca, a 3βτβΜ npocce/mpyeTCHnepee meju>, otípa3OBaHHy» HapyxHoft noBepxHocTHo BepxHerocejyia n KOJimeotípa3HHM BuciynoM (hjih BttcTynaMH) HHJKHeroce^Jia, b oánacTB HopMajiLHoro ηβΒΛβωΐΗ. lípu stom nponc-xosht Ke3HHTerpamifl ιυιβτοκ 3a οηβτ neKOMnpeccmi. Patío-nee naBJíeHne b ysJíe ae3HHTerpaiw oóecnenuBaeTCH HaTnroMperyjmpoBOHHHX bhhtob, peiyjmpyeTca BpynHym η κοητρολη-pyeTcn no MaHOMeTpy. ycraHOBKH neKOMnpeccHOHHoS j[e3HHTerpaiíHH xapaKTe-pH3y»TCH nocraTOHHo BucoKofi ΒφφβκτΗΒΗοοτΗο paspymeHiw 245736 -4- ΚΛβΤΟΊΗΗΧ ΟόΟΛΟΗβΚ ΠρΗ 3HaHHTeJU>HO ΜβΗΜΙϋΧ ΠΟ ΟρβΒΗβΗΗΒC yCTaHOBKSMH ZtpyrMX ΤΗΠΟΒ MeraJUOeMKOCTBB Η 9ΗβρΓΟ3βΤ-paiaMH. ycTaHOBKK .padoTax® npa οτηοοητθλβηο ηθββοοκηχ3HaHeHHHx padonero λώβλθι-ιηη (80-200 aru). Μθτολ λθκομπ-peccim, HcnojiLSOBaHHHfi b 9TKX ycTaHOBKax, odecneHHBaerB03M0SCH0CTB MaCitlTatínpOBaTB HX RO Πρ0Η3Β0ΠΗΤβΛΒΗ00ΤΗ, Tpe-tíyeMoa b npoMUDUieHHóM npoH3BoncTBe. K -He^ocTaTKaw onncaHHHx BapHaHTOB ycraHOBOK ^βκοΜ-npeccnoHHOft jzesHHTerpamiH cjienyer οτηθοτη cjioshoctb koh-CTpyitiflifl y3Jia se3HHTerpanHH h H6oóxojxhmoctb pynHoS pery-jmpoBKH βθβλθηηη Ha yene nesaHierpanaH b nponecce padOTHyciaHOBKH. ΕηηκηΒπιημ τθχηηήθοκημ peweHHeM κ npejyKaeHHOMy noTexHinecKoíS cymHocTH ά aocTuraeMoMy δφφβκτγ ηβληθτοηyoraaoBKa rjisí ne3HHTerpanHH ΚΛβτοκ MincpoopramisMOB, co-nepRamaa cMecHieju. cycneHSHH MHKpoopraHHáMOB c ra3OM hne3MHTerpaTop, βκλβηθβπιη^ Kopnyc, ycTaHOBjíeHHy® b ηθμ cBO3MOXHOCTB® ββ ΒβρΤΗΚβΛΒΗΟΓΟ ΠβρβΜβΙΙΙβΗΗΗ T/ITRS» ΡΗ3ΜΘΠξβΗ-Hy® b ΗΗΚΗβϋ Bac τη Kopnyca npocceJiLHy® mafidy, otípa3y®my®c Hraofl padony® ®θλβ, ipydonpoBoa jvw nosBO«a b neanHTerpa·lop HCXOHHOfi HaCHmeHHOfi ra30M CycneH3HH H CtíopHHK ΛΘ3ΗΗ-rerparopa [ 3 ]. J(e3HHTerpaiop conepstHT paanejieHHHe MeMtípaHofi sana»-my® h padoay» κβΜβρκ; padonan KaMepa odpa3OBafla nocjieno-BaieJiMO coefiHHeHHHMH Mesmy codoft odeaafiKofí, peryjuipy®-ihjjm φπβΗΐξθΜ h Kopnyc om ApoccejiBHofí mafiÓH, BHyTpn pádovíeflxaMepu ycTaHOBJaefía nr.ua, yKpenjieHnaji Ha MeMdpaHe h onapa®-maaca Ha cejyio ηγλη b ApoccejiBHOfi maftde. 3ana®maH KaMepaΗΜ66Ϊ* BXOJIHOfi ΚΗΗ8Λ RJIR HORŠÍMI ΟΚΘΤΟΓΟ Ta3a H BHXOflHOÍiKaHaji rrb ctípoca saBJíeHHH, á padoaaa KaMepa ΗΜββτ doKO-boS Bxoflfío# KaHaji rjir horami HacHmeHaofi raeoM cycneHSHHΚΛΘΤΟΚ.
YcTaHOBKa padOTaeT cxenyroiUHM otípa3OM. C noMomao csa-τογο rasa co3Aa®T patíoaee jtaBJieHne ε 3ana®inefl KaMepesesHHTerpaTopa. 3βτβΜ c πομοπιηο Hacoca-nosaiopa b ομθοη-Teju> nonaror cycneHsu® ιυιβτοκ MHKpoopríiflHSMOB. Πρκ jiocth- 245736 -5- X8HHH Ρ00ΟΊΘΓΟ flaBJíeHHH (ΟΚΟΛΟ 100 3ΤΠ) Β CM8CHT8JU» Ηθ-ίηηθιοτ noflasaTB cxaraS ra3, h ηθοηαθηηβη ra3OM cycneH-3HH nocTynaeT Ηβρβ3 Βχο,πΗοϋ Κ3Η3Λ β padonyio KaMepy αθ-3HHTOrpaTopa. B HCXOflHOM ΠΟΧΟΗΘΗΗΗ, ΚΟΓΑΘ CSaTHÉ Γβ3 ΠΟ®Η Β 3Θ-AW» KaMepy fle3HHTerpaTopa, βηχοαηοθ οτβθperné Η3 θγοpadoneS κθΜβρκ 3βκρΗτο κγλοΑ, onapaicmefica Ha ceflno βípoccejihHoa maiíde. lípa πρθβμήθηπη flaBJíeHHH b padonefiκβΜθρβ no οτηοπθηηκ> k naBJíeHino b 3aflaiome:3 aaiaepe bpe3yjiBTaTe padora Hacoca-flO3aTopa MeMópaHa, pa3flejunomaaΚβΜθρΗ, ΠΟΑΗΗΜΘΘΤΟΗ ΒΜΘΟΤΘ C γκρβΠΛΘΗΗΟΗ Ha Hefl HTJIOÍÍ,npn stom oópa3yeTcn meju, Meamy 3annpafamnM kohuom htjih nθθ οθααομ, n nepe3 3Ty μθλβ cycneH3HH BHdpacHBaeTOH bctíopHHK fleanHTerpaTopa. Πρη &tom 3a οηθτ αθκομπρθοοηηnpOHCXOflHT ne3HHTerpanHH ΚΛΘΤΟΚ. PaBHOMepHOCTB Ρ83ΡΗΛΚΗcycneH3Hn oetok odeoneηηβθιοτ c iiomoiui,® peryMpyianeroφΗΘΗΐξθ. ycTaHOBKa padOTaeT b HenpepuBHOMpeaomié ,n odecne-nimaeT Ae3HHTerpaw ΚΛΘΤΟΚ MHKpoopraHHBMOB Ha 70-75$.
OflHaKO ΡΘΟΠΟΛΟΚΘΗΗΘ HTJIH BHyTpH ρθΰΟΗΘ.ϋ ,ΚΘΜβρΗ HdoKOBoft bboa cycneH3HH β pa doryio KaMepy β οπηοθηηομyoTpofflcTBe πρηβολητ k 3aKpyHHBaHmo ποτοκθ cycneH3nn bKaHaJie, 0dpa30BaHH0M οτβΗΚθΜη paóonefl κθμθρη h htjioíí,a Tarate κ 3aKpynHBaHHio cTpyn cycneH3HH npn BHdpoce eeΊβρθ3 padoqyio meju>, otípa3yeMy» nrjiofi η cewiOM b flpoccejiB-Hofl maflde. B pe3yju>TiT8 yBeJiHHHBaeTon βρθμη cdpoca fláBJíe-HHH B CycneH3HH, ΗΤΟ 0ΗΗΚ3ΘΤ ΟΤΘΠΘΗΒ ΑΘ3ΗΗΤΘΓρ8ηΗΗ. KpO-ΜΘ Toro, flBHxeHne cTpya cycneHSHn Ha βηχοαθ H3 fl83HHTerpa-TOpa, odycJioBfleHHoe φορΜϋίί meJiH Me&fly ητ Jiofl h ee csajiom,ΗΘΠΡΘΒΛΘΗΟ K OCH HTJIH, HTO yxyflflaeT ρβΟΠΗΑΘΗΗΘ BHXOflfUHeflCyon8H3HH Η, K8K CJU}flCTBH8, ΟΤΘΠβΗΒ fl83HHT8TpaHHH.
KoHCTpyKHHH íB3KHT8rpaTopa, cocTOHJnan ® nerapexΠΟΟΛΘΛΟΒΒΤΘΛΒΗΟ CO8flHH8HHHX M8Xfly COÓOfl 8Λ8ΜΘΗΤ0Β -3anawfl n padonefl κθμθρη, peiyjiHpyiomero $naHo;a η κορ-nyca flpoccejiBHOfl maftdH - ολοκηθ ahh peryjmpoBaHHH npnΗ3ΜΘΗΘΗΗΗ ρθΜΜΟΒ pa d O ΤΗ H 38ΜΘΗ8 3ΛΘΜΘΗΤ0Β. 245736 -6-
UejIMO Η3ΟόρβΤβΗΗΗ ΗΒΛΗΘΤΟΗ ΠΟΒΚΠΙβΗΗβ ΟΤβΠβΗΗ βθβΗΗ-TerpaUHH npH ΟΗΗΟΒρβΜβΗΗΟΜ ynpomeHHH KOflCTpyKlíHH. ΠΟΟΤΗΒΛβΗΗβΗ ΙίβΛΒ flOCTuraeTCH ΤΘΜ, HTO yCTaHOBKaflJIfl ,He3HHTerpauHH MHKp00praHJH3M0B COflepMT ΟΜβΟΗΤβΛΒcycneH3HH MHKpoopraHH3MOB c ra3OM η ne3HHTerpaTop, BKJDO-πωοιρβ Kopnyc, ycTaHOB#eHHyio b ηθμ c bo3Mo>khoctbk> eeBepwiKajiBHoro nepeMemeHHH Wi pa3MeineHHyio b ηοηθΑ nac-th Kopnyca ApoccejiMyio uiaAdy, oópa3y»my® c ηγλοΑ patíonyBmeji£> TpytíonpoBOjt jyia no^Bona b ^esk^rerpaTop HcxonHoSHachimeHHofl ra30M cycneH3HH h cdopHHK aesHHTerpaia. Otjih-ΗΗβΜ flBJxnercH to, hto Kopnyc caaóxeH pacnojioaceHHoS hojejipoccejiLHoíí uiafitíoa coocho nrjie BTyjmofl, cjlyaameA λλη πολ-Bojia acxoflHoS cycneH3HH b padony» ujejiB h coejiHHSHHoíi cyKa3aHHKM BHine rpydonpoBonoM, πρπ θτομ Kopnyc βηποληθη bβηε;θ CTasaHa, HMeratero Ha óokoboS noBepxHOCTH okhh raBHÓpoca £e3HHTerpaTa b cdopHHK. H3oópeTettne ποαοηηθτοη ηβρτθικοΜ, Ha κοτοροΜ φκτ.ΙcxsMaTHHHo n3oópa)seT odmufí bují npejvoaraeMoíi ycTaHOBKH,Φηγ.2 - προΛΟΛΒΗΗβ pa3pe3 jte3HHTerpaTopa.
YcTaHOBKa jym Ae3HHTerpauHH κχβτοκ MHKpoopraHH3MOBοοΛβροτ cMecHrejiB I cycneH3HH κλθτοκ MHKpoopraHUSMOBCO CiSaTHM Γ8.30Μ, CHaÓJSeHHHfi flaTHHKOM H peryjIHTOpOM ypOB-hh 3anojiHeHHH (Ha πβρτβκβ He noKaeaHu) η μηηομθτρομ hjihΚΟΗΤΡΟΛΗ ΕΗΒΛΘΗΗΗ H CoeflHHeHHHfl TpyóOHpOBOflOM 2 C fle3Hfí-TerpaTopoM 3, κοτορκΑ πομθπ;θη b cóophhk 4 ne3HHTerpaTopa.K cMecHTejno I ποκκώοηοηη TpyóonpoBon 5 caaToro ra3a hrpyóonpoBOE 6 cycneH3HH, nocTynaratefi ot Hacoca-no3aiopa 7.
Hjui jtynmero KOHTaKTa ra3a c cycneH3nefi ΟΜβΟΗΤβΛΒ IMOKteT óbitb flonojiHHTejiBHO odopynoBan neperoponKawiH h/hjihnepeMeniHBatomHM ycrpoficTBOM; η&Η odecneneHHH τθρμοοτητηρο-BaHHH CMeCHTeJIB I MOÍKST tíHTB CHaÓKeH ΤβΠΛΟΟόΜβΗΗΗΚΟΜ. j(e3HHTerpaTop BKjnouaeT Kopnyc, b BepXHeS nacin 8κοτοροΓΟ ycTanoBJíeHa c bo3Mokhoctbí3 ee BepTHKaJiBHoronepeMemeHHH nrjia 9·, a β ηηκηθΑ nacrn 10 pasMenjefía.flpoc-’cejtBHa.fi r^Ada II, otípasyratan c arjtoA padonyro mejiB; KopnyccnatísceH pacnojtosteHHofi no# ApoccejiBHoJí. maitdoít II coochonrjie 9 BTyjiKOfl 12, οοθηηηθηηοΑ c πομοπφιο č&jiaHpa 13 h 245736 -7- ynopHHX UfflHJIOK 14 C TpyÓOnpOBOflOM 2 flJIfl nOflBO^a HCXOflHOÍÍHacuuieHHOíí ra30M cycneH3nn, πριι θτομ Kopnyc ββιποληθη bBEme cTaicana, tuvteromero Ha óokoboíí ποΒορχίΐοοτη oKíia jywBHÓpoca fle3HHTerpaTa b cóophmk 4.
BepXHHH 8 II HHKHHH 10 HaCTH KOpnyCa COenilHeHb! C B03-MOKHOCTBJO ΗΘΚΟΤΟρΟΓΟ ΠθρβΜΘΙΙ’ΘΗΗΒ OTHOCHTeJiBHO flpyr £pyra,HanpwMep, c πομοπιβιο pesBÓbi; nrna 9 μοχθτ óbitb BunojmeHaB BW ΟΟΤρΟΚΟΗΘΗΗΟΓΟ CTCpJKHH ΜΛΗ B Bime CTCp/KHH C UiapHKOMycraHOBJíeHHWM Ha ero saimpaiomeM κοΗΐι,θ.
YcTaHOBKa paóoraeT cnenyroutrn oópa3OM. lípu ποληοοτμοnepespuTOM mtjioíí 9 οτΒβροτκκ npoccejitHOM waQóbi II nesHH-TerpaTopa 3 b cMecoejih I no rpyóoripoBony 6 c πομοιιιμο Ha-coca-noeaTopa 7 ηββμηηιοτ HenpejiHBHO nonaBaTt cycneH3moKJieroK MHKpoopraHH3MOB, Týna xce no TpydonpoBony 5 nonaioTcjKarufl ra3 (asoT, Bosnyx, yrjieKMCjioTy h npyrHe),KOTopHfí,tíaptíoTHpyn nepe3 cnoíl cycnensun, HacbmiaeT Kiinnyio $a3yCycneH3HH Η ΚΛβΤΚΗ MHKpOOpraHMSMOB. ΥρΟΒΘΗΒ cycneH3HH BCMecnrejie I npenBapMTenBHo aanaioT h b nantnefinieM ποηηθρ-HÍHBaiOT noCTOHHHbíM C ΠΟΜΟΙΙφΚ) flaTBHKa II peryjIHTOpa ypOBHH(Ha nepřeme He noKasaHH) τθκημ oópasowí, htoóh cooTHOiue-Hne Mesny oóteMOM, 3aHHMaeMHM ra3OM, ii oóneMOM cycneH3nncocTaBWio ot 1:3 no 3:4. Paóonee naBnemie nonnepjMBaioTb npenenax ot 80 no 200 ara.
Ho nocTHMceHHH sanannoro ypoBHH κιιηκοοτη b ομθοητθλθI BepTHKajiBHbM nepeMemeHHeM ηγλη 9 no3HHTerpaTopa 3 npn-οτκρΗΒθίοτ OTBepcTne npoccenLHoij iuaSóbi II. Bo3BpaTHO-no-CTynaTejiLHoe nepeMenteHne ηγλη μοκθτ ocyuiecTBJíHTBCH spyn-nyio ηλη aBTOMaTiinecKH. B otípasoBaBiuyiocH Meutny htjioS 9 hee cennow b npoccentfioS uiaSóe II ment H3 cMeciiTejin I noTpydonpoBony 2 nannHaeT nocTynaTt HacuineHíian ra30McycneH3iwi MiiKpoopraHiisMOB. nepes odpa30BaHHyio ment cyc-nensnn c óojibiuom οκοροοτυο BbiópacHBaercfl b cóophkk ηθ-sHHTerpaTa 4, πριι stoi/ nannenne cnumaeTCH no aTMoccpep-noro h 3a cnei neKOMnpeccmi προκοχοηπτ pa3pynieHiie κλθτοκMHKpOOpraHHSMOB.
Jl&nec ycraHOBKa paóoraeT npn HenpepiiBíiofi nonane 245736 -8-
CycneH3HH MHKpOOpraflH3MOB H CKaToro raea Η ΟΤΒΟ,Π,Θ ΓΟΤΟΒΟ-ro ji;e3HHTerpaTa, ηθ^θμηο odecneniiBan ηθβηητθγρθιηπο ΐϋΐβτοκHa 75-80$.
Thkhm otípa3OM, ncnojii.3OBaHiíe npeflJiaraeMoíí yciaHOBKHotíecneHHBaeT noBtnneme cienenu jie3HHrerpaiH«i Ha 5-8$ áaaro-AapH yjiynmeHmo ycjiOBMíi Biitípoca cipyn cycneH3M kjistoknepea padaným mejit fle3MTerpaTopa.
OsHOBpeMeHHo ynpomeHa KOHCTpyKW ycTaHOBKH no cpas-ΗΘΗΗΚ) C M3B6CTH0fl 3a ΟΗΘΤ yMeHHlieHHfl KOJUneCTBa KOHCTpyK-thbhhx 3Λ6ΜβΗΤ0Β, OTcyTCTBHH sa^ammea KaMepti, peryjiwpym-mero $jiaHna, η3Μθηθηηη B3aHMHoro pacnojioaceiuiH ποαβοληπιθγοCycneH3HK Kaima Η íIBJIhl, ΗΤΟ Π03Β0ΛΗβΤ ΛΘΓΚΟ ΠρΟΗβΒΟ,ΕξΗΓΒperyjiupoBicy m,e3HHrerparopa, 3ameny βγμι h ^poccsjílhobiua0dn no Mepe hx H3Hoca, a τβκκβ 3βτομβτη3ΗΡοββτβ nponeccae3HHTerpaiíHH. 245736 -9-
ΦΟΡΜΟΑ MSOEPETEHHH ycrafíOBKa ajih fle3HHierpainiH ,κλθτοκ MKKpoopraHM3MOB,coflepatanian CMecmejn, cycneH3im MHKpoopratuieMOB c ra3OMh jte3HHTerparop, BKjnonaíomHfi Kopnyc, ycTaHOBJíeHHy» b ηθμC BO3MO3KHOCTBK) ββ ΒβρΤΗΚΘΛΒΗΟΓΟ ΠθρθΜβΙΙίθΗΗΗ Hrjty, pa3-Memennyio b HnmeS flacTH Kopnyca flpocceflBHyio inafidy, odpa-ayroqyK) c nrjiofí padonym mejit, TpydonpoBOfl ajih noflBOfla bfle3HHTerpaTop ncxoflHofí HacumefíHOň ra30M cycnen3HH h cdop-hhk fle3nnTerpara, oTJimaiomaHCH tom, hto, c nejisio ποβμιιθ-hhh cieneHH fle3nnrerpaimn h ynpomeinm KOHcrpynmra, κορ-nyc CHatíaeH pacnojioaeHHofl nofl flpocce.ni.Hofi mafidofi coochonrae BTyjncofi, cjjyaameS αλη noflBOfla ncxoflHofi cycneH3HHb padonyio meju. n coeflHHeHHofi c yKa3aHHHM BHine Tpydonpo-βοαομ, πρκ 3T0M Kopnyc βκποληθη b Bijfle craKaHa, HMeromeroHa όοκοΒοβ noBepxHocTH OKHa αλη BHtípoca fle3HHTerpaTa bCdopHHK. 245736 10-
AHHOTAW
HacToamee asodpeieHiie othochtch k MHKpodHOJiornae— CKOfi ΠρΟΜίωίΛβΚΗΟΟΓΗ H ΠρβΛΗβ3ΗβΗβΗΟ JCJECS P.a3pyffleHJW ΚΛβ-TOK MHKp00praHH3M0B, ΤΘΚΗΧ,ΚΘΚ SPOXSH, daKTepHH, ΒΟ£Ο-POCJIH, rpiIÓH, flJIfl ΒΗΑΘΑΘΗΗΗ BHyTpHKJieTOAHBX ČeJEKOB, Hy-ΟβΗΗΟΒΗΧ KHCJIOT, φβρΜΘΗΤΟΒ H ApyrHX φΗΒΗΟΛΟΓΗΗβΟΚΗ ΘΚ-THBHHX BfimeCTB.
UejIBK Η300ρβΤβΗΗΗ ΗΒΑΗβΤΟΗ ΠΟΒΙΟΙίβΗΗΘ ΟΤΘΠΘΗΗ Αβ8ΗΗ-TerpaiíHH πρκ οαηοβρθμθηηομ ynpomeHHH KOfíCTpyKujm.
YcTaHOBKa ajih AeanHierpamui κλθτοκ μηκροοργηηη3Μοβ
COflepOT ΟΜβΟΗΓΘΛΒ CyCIieH3HH ΜΗΚρ00ρΓΗΗΗ3Μ0Β C ΓΗ30Μ H
HesHHTerpaiop, BKJnoHaroiUHfi Kopnyc, ycraHOBjíeHflyB β ηθμ cB03MOKHOCTHO ee βθρτηκθλβηογο nepeMemeHHa Hivny, paaMemeHHyiob HKRHefi naciH Kopnyca ApoccejiiHyio niaatíy, odpaayranye oηγλοΗ padonyio meju>, rpyóonpoBOfl ajih noABoaa b Ae3HHTer-ρβτορ hcxoahoS KacwHHofí ra30M cycneasmi h cdopHHK Αθ-3HBTerpaTa, npmeM Kopnyc CHatí«eH pacnojiosceHHOfí πόαApoccejisnofl mafttíofi coocho rcrjie BTyjmoí?, CAyatamefl ajw ποα-boas hcxoahoA cycneH3HH b padonyro meju> π. coeAHHeaHOfl cyxasaHHUM Etane TpydonposoAOM, πρκ 3τοι» Kopnyc βηποληθηβ bha® CTaKaHa, HMeromero Ha ΰοκοΒοϋ ποΒβρχΗοοτκ οκηηΑΑΗ BHÓpOCa Αθ3ΗΗΤβΓρβΤβ B CdopHHK. 245736

Claims (1)

  1. 'Zařízení na kontinuální dezintegraci buněk mikroorganismů, obsahující směšovač suspenze mikroorganismů a'plynem a dezintegrátor, zahrnující pouzdro, jehlu umístěnou v něm s možností jejího vertikálního posunu,· škrtící podložku uloženou v dolní části pouzdra, vytvářející s jehlou pracovní mezeru, potrubí pro přívod základní plynem nasycené . . suspenze do dezintegrátoru a nádobu dezintegrátu,· vyznačující se tím, že pouzdro je vybaveno vložkou uloženou pod škrticí podložku souose s jehlou, pro přívod základní suspenze do pracovní mezery a spojenou s výše uvedeným potrubím, přičemž pouzdro je provedeno ve formě kalichu, mající na boční ploše otvory pro odstřik dezintegrátu do •nádoby··.
CS823379A 1981-11-13 1982-05-10 Apparatus for continual desintegration of microorganism cells CS245736B1 (en)

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AU604684B2 (en) * 1986-03-20 1991-01-03 Gen-Probe Incorporated Method for releasing RNA and DNA from cells
DE3612453A1 (de) * 1986-04-14 1987-10-15 Diessel Gmbh & Co Vorrichtung zur kultivierung von zellkulturen
WO1991001367A1 (en) * 1989-07-20 1991-02-07 Bioeng, Inc. Supercritical fluid disruption of and extraction from microbial cells
JP2966904B2 (ja) * 1990-08-08 1999-10-25 株式会社日立製作所 細胞の処理方法、および処理装置
DE4241154C1 (de) * 1992-12-07 1994-03-17 Lancaster Group Ag Verfahren zum Aufschluß von Zelldispersionen oder Zellsuspensionen mittels Ultraschallbehandlung zwecks Gewinnung von Zellinhaltsstoffen
US5620730A (en) * 1993-09-09 1997-04-15 Van Noort; Gerard Method of enhancing shelf-stability of an edible biological product
US7033813B2 (en) 2001-09-05 2006-04-25 Trevor P Castor Inactivated vaccines for aids and other infectious diseases
US7527734B1 (en) * 2005-11-15 2009-05-05 Shepherd Samuel L Rapid non-equilibrium decompression of microorganism-containing waste streams
TWI349651B (en) * 2008-03-20 2011-10-01 Ind Tech Res Inst A water quality purifying system
US10159980B2 (en) * 2013-08-02 2018-12-25 All Cell Recovery LLC Systems and methods for recovering blood cells, in a controlled environment, for storage

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FR532555A (fr) * 1921-03-22 1922-02-07 Robinet pour brûleurs d'appareils à gaz et autres applications
GB668055A (en) * 1950-08-08 1952-03-12 Flexibox Ltd Improvements relating to stop valves
US3165266A (en) * 1962-02-07 1965-01-12 Sorvall Inc Ivan Cell fractionator apparatus and method
SE334212B (cs) * 1965-09-17 1971-04-19 Biox Ab
DE1958962A1 (de) * 1969-11-24 1971-05-27 Niro Atomizer As Regelventil
SU492118A1 (ru) * 1973-01-12 1978-04-15 Всесоюзный научно-исследовательский институт биосинтеза белковых веществ Установка дл дезинтеграции микроорганизмов
JPS5328989B2 (cs) * 1974-05-27 1978-08-17
SU602550A1 (ru) * 1974-12-30 1978-04-15 Всесоюзный научно-исследовательский институт биосинтеза белковых веществ Установка дл непрерывной дезинтеграции клеток миктоорганизмов
SU602551A1 (ru) * 1975-12-03 1978-04-15 Всесоюзный научно-исследовательский институт биосинтеза белковых веществ Установка дл непрерывной дезинтеграции клеток микроорганизмов
US4084757A (en) * 1976-08-05 1978-04-18 Vladimir Jurievich Rakitin Apparatus for continuous disintegration of cells of microorganisms
FR2361466A1 (fr) * 1976-08-13 1978-03-10 Vnii Biosinteza Belkovykh Procede de desintegration en continu de cellules de micro-organismes et unite pour la mise en oeuvre dudit procede

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DE3226016A1 (de) 1983-05-19
FR2516538A1 (fr) 1983-05-20
JPS627831B2 (cs) 1987-02-19
FR2516538B1 (fr) 1986-06-20
SU1116054A1 (ru) 1984-09-30
US4476225A (en) 1984-10-09
CH655514B (cs) 1986-04-30
JPS5889177A (ja) 1983-05-27
DE3226016C2 (de) 1986-10-02

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