CR20160513A - Deteccion de fenotipo de cobertor en palma - Google Patents

Deteccion de fenotipo de cobertor en palma

Info

Publication number
CR20160513A
CR20160513A CR20160513A CR20160513A CR20160513A CR 20160513 A CR20160513 A CR 20160513A CR 20160513 A CR20160513 A CR 20160513A CR 20160513 A CR20160513 A CR 20160513A CR 20160513 A CR20160513 A CR 20160513A
Authority
CR
Costa Rica
Prior art keywords
phenotype
covertor
palma
detection
somaclonal
Prior art date
Application number
CR20160513A
Other languages
English (en)
Inventor
Nathan D Lakey
Meilina Ong Abdullah
Ooi Siew Eng
Michael Hogan
Leslie Low Eng Ti
Rajinder Singh
Rajanaidu Nookiah
Nan Jiang
Rob Martienssen
Ravigadevi Sambanthamurthi
Jared Ordway
Steven W Smith
Original Assignee
Malaysian Palm Oil Board
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Malaysian Palm Oil Board filed Critical Malaysian Palm Oil Board
Publication of CR20160513A publication Critical patent/CR20160513A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/164—Methylation detection other then bisulfite or methylation sensitive restriction endonucleases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Alarm Systems (AREA)

Abstract

Se proporcionan métodos, composiciones, kits y códigos de programas informáticos para predecir la anormalidad somaclonal (p.ej, un fenotipo Cobertor) en una clasificación de planta o plantas y en base a la presencia o ausencia predicha de anormalidad somaclonal
CR20160513A 2014-05-02 2015-04-30 Deteccion de fenotipo de cobertor en palma CR20160513A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461988132P 2014-05-02 2014-05-02
US201462091471P 2014-12-12 2014-12-12
PCT/US2015/028646 WO2015168470A2 (en) 2014-05-02 2015-04-30 Mantle phenotype detection in palm

Publications (1)

Publication Number Publication Date
CR20160513A true CR20160513A (es) 2017-05-16

Family

ID=54354832

Family Applications (1)

Application Number Title Priority Date Filing Date
CR20160513A CR20160513A (es) 2014-05-02 2015-04-30 Deteccion de fenotipo de cobertor en palma

Country Status (7)

Country Link
US (2) US9984200B2 (es)
EP (1) EP3137491A4 (es)
CN (1) CN106471008B (es)
CR (1) CR20160513A (es)
MY (1) MY193347A (es)
SG (1) SG11201609025TA (es)
WO (1) WO2015168470A2 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CR20160513A (es) 2014-05-02 2017-05-16 Malaysian Palm Oil Board Deteccion de fenotipo de cobertor en palma
CN106868159B (zh) * 2017-03-20 2020-12-08 西北农林科技大学 一种用于鉴定番茄萼片形态的ssr分子标记、引物及应用
CN108396073B (zh) * 2018-03-23 2021-06-18 中国热带农业科学院椰子研究所 一种鉴别油棕种苗Mantled畸形的方法
CN110276775B (zh) * 2019-07-08 2021-05-04 浙江理工大学 一种基于机器视觉的番茄穴盘苗嫁接切削参数识别方法

Family Cites Families (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4910146A (en) 1988-07-18 1990-03-20 Hartmans Plants, Inc. Automated plant culture proliferation system
US6033854A (en) 1991-12-16 2000-03-07 Biotronics Corporation Quantitative PCR using blocking oligonucleotides
US5405760A (en) 1992-04-30 1995-04-11 New England Biolabs, Inc. Process for producing recombinant McrBC endonuclease and cleavage of methylated DNA
WO1995006137A1 (en) 1993-08-27 1995-03-02 Australian Red Cross Society Detection of genes
US5786146A (en) 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US6307123B1 (en) 1998-05-18 2001-10-23 Dekalb Genetics Corporation Methods and compositions for transgene identification
US6331393B1 (en) 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
US6180349B1 (en) 1999-05-18 2001-01-30 The Regents Of The University Of California Quantitative PCR method to enumerate DNA copy number
US6646264B1 (en) 2000-10-30 2003-11-11 Monsanto Technology Llc Methods and devices for analyzing agricultural products
US7367155B2 (en) 2000-12-20 2008-05-06 Monsanto Technology Llc Apparatus and methods for analyzing and improving agricultural products
CN100558900C (zh) 2001-02-09 2009-11-11 孟山都技术有限公司 用表型标记鉴定种子或植株
DE10130800B4 (de) 2001-06-22 2005-06-23 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung mit hoher Sensitivität
US6673595B2 (en) 2001-08-27 2004-01-06 Biocrystal, Ltd Automated cell management system for growth and manipulation of cultured cells
US6880771B2 (en) 2002-02-01 2005-04-19 Monsanto Technology Llc Axially reciprocating tubular ball mill grinding device and method
BR0309017B1 (pt) 2002-04-04 2015-02-18 Monsanto Technology Llc Máquina automática para manusear e manipular peças individuais de material particulado
WO2003100381A1 (en) 2002-05-24 2003-12-04 Monsanto Technology Llc. Seed coring system and method for arranging seed cores for analysis
CA2489360C (en) 2002-06-26 2013-01-29 Cold Spring Harbor Laboratory Methods and compositions for determining methylation profiles
GB0222846D0 (en) 2002-10-03 2002-11-06 Choo Yen Cell culture
CA3074799C (en) 2003-03-28 2022-12-06 Inguran, Llc Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm
WO2005031367A2 (en) 2003-09-23 2005-04-07 Monsanto Technology Llc High throughput automated seed analysis system
ATE550438T1 (de) 2003-10-21 2012-04-15 Orion Genomics Llc Verfahren zur quantitativen bestimmung der methylierungsdichte in einem dna-locus
US7703238B2 (en) 2004-08-26 2010-04-27 Monsanto Technology Llc Methods of seed breeding using high throughput nondestructive seed sampling
MX2007002307A (es) 2004-08-26 2007-04-16 Monsanto Technology Llc Prueba automatizada de semillas.
NL1030072C2 (nl) 2004-09-30 2006-10-03 Malaysian Palm Oil Board Nieuwe B-type gen uit oliepalm.
AU2006244254B2 (en) 2005-05-09 2012-02-02 Monsanto Technology Llc Custom planter and method of custom planting
US20070048872A1 (en) 2005-08-26 2007-03-01 Deppermann Kevin L High throughput screening of fatty acid composition
US8028469B2 (en) 2006-03-02 2011-10-04 Monsanto Technology Llc Automated high-throughput seed sampler and methods of sampling, testing and bulking seeds
US7998669B2 (en) 2006-03-02 2011-08-16 Monsanto Technology Llc Automated contamination-free seed sampler and methods of sampling, testing and bulking seeds
US20070224626A1 (en) * 2006-03-09 2007-09-27 Orion Genomics Llc Comprehensive DNA methylation profiling in a human cancer genome identifies novel epigenetic targets
US8076536B2 (en) 2006-05-12 2011-12-13 Monsanto Technology Llc Methods and compositions for obtaining marker-free transgenic plants
CA2656676C (en) 2006-06-28 2016-04-26 Monsanto Technology Llc Small object sorting system and method
WO2008103609A1 (en) 2007-02-23 2008-08-28 Monsanto Technology Llc Agricultural sample grinder
BRPI0808665B1 (pt) 2007-03-09 2022-05-10 Monsanto Technology Llc Métodos e construções para produção de plantas de soja, algodão ou canola transgênicas usando seleção de espectinomicina
US8452445B2 (en) 2007-04-24 2013-05-28 Pioneer Hi-Bred International, Inc. Method and computer program product for distinguishing and sorting seeds containing a genetic element of interest
US20110079544A1 (en) 2009-10-01 2011-04-07 Pioneer Hi-Bred International, Inc. Method for sorting resistant seed from a mixture with susceptible seed
BRPI0811990A2 (pt) 2007-05-31 2014-09-23 Monsanto Technology Llc Separador de semente
US8076076B2 (en) 2007-08-29 2011-12-13 Monsanto Technology Llc Systems and methods for processing hybrid seed
BRPI0815810A2 (pt) 2007-08-29 2014-10-07 Monsanto Technology Llc Métodos para incorporação de genes múltiplos em uma planta de cultura
WO2009079505A2 (en) 2007-12-17 2009-06-25 Pioneer Hi-Bred International, Inc. Apparatus, method and system for creating, handling, collecting and indexing seed and seed portions from plant seed
US8925762B2 (en) 2008-07-02 2015-01-06 Monsanto Technology Llc High speed counter
US8685321B2 (en) 2008-08-05 2014-04-01 Monsanto Technology Llc Automated multi-station small object analysis
WO2010056107A2 (en) * 2008-11-13 2010-05-20 Malaysian Palm Oil Board Method for identification of a molecular marker linked to the shell gene of oil palm
MX347314B (es) 2010-03-26 2017-04-20 Monsanto Tech Llc * Sistemas y métodos automatizados para clasificar objetos pequeños.
US20130192135A1 (en) 2010-03-26 2013-08-01 Monsanto Technology Llc Systems and methods for selection of individual seeds and placement onto seed tape
WO2011119390A1 (en) 2010-03-26 2011-09-29 Monsanto Technology Llc Systems and method for selection of seed and placement on seed tape
US9357710B2 (en) 2010-03-26 2016-06-07 Monsanto Technology Llc Intelligent selection and remapping of ellepots
BR112013001330B1 (pt) 2010-07-20 2019-04-02 Monsanto Technology Llc Sistemas de retirada de amostra de semente e método automatizado para remover amostras de tecido de sementes
MY154507A (en) 2012-03-19 2015-06-22 Malaysian Palm Oil Board Gene Controlling Shell Phenotype in Palm
EP2787004A1 (en) * 2013-04-03 2014-10-08 Malaysian Palm Oil Board A method for predicting a "mantled" phenotype in oil palm plants and minimizing the percentage of oil palm plants with a "mantled" phenotype in a culture, and polynucleotide to be used therein
SG10202012194SA (en) 2013-04-08 2021-01-28 Malaysian Palm Oil Board Gene controlling fruit color phenotype in palm
CR20160513A (es) 2014-05-02 2017-05-16 Malaysian Palm Oil Board Deteccion de fenotipo de cobertor en palma

Also Published As

Publication number Publication date
CN106471008A (zh) 2017-03-01
SG11201609025TA (en) 2016-11-29
CN106471008B (zh) 2021-04-09
WO2015168470A3 (en) 2016-01-21
US9984200B2 (en) 2018-05-29
US20180314794A1 (en) 2018-11-01
US20150315662A1 (en) 2015-11-05
WO2015168470A2 (en) 2015-11-05
US11632922B2 (en) 2023-04-25
EP3137491A2 (en) 2017-03-08
BR112016025562A2 (pt) 2018-07-03
MY193347A (en) 2022-10-06
EP3137491A4 (en) 2018-02-21

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