CO6700839A2 - N-aciltransferasa de glicina terapéutica recombinante - Google Patents

N-aciltransferasa de glicina terapéutica recombinante

Info

Publication number
CO6700839A2
CO6700839A2 CO13057288A CO13057288A CO6700839A2 CO 6700839 A2 CO6700839 A2 CO 6700839A2 CO 13057288 A CO13057288 A CO 13057288A CO 13057288 A CO13057288 A CO 13057288A CO 6700839 A2 CO6700839 A2 CO 6700839A2
Authority
CO
Colombia
Prior art keywords
expression
glyat
acyltransferase
glycine
host
Prior art date
Application number
CO13057288A
Other languages
English (en)
Inventor
Lodewyk Jacobus Mienie
Dijk Alberdina Alke Van
Christopher Petrus Sthephanus Bandenhorst
Der Sluis Rencia Van
Original Assignee
Univ Northwest
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Northwest filed Critical Univ Northwest
Publication of CO6700839A2 publication Critical patent/CO6700839A2/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/45—Transferases (2)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/02—Antidotes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01013—Glycine N-acyltransferase (2.3.1.13)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Obesity (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Esta invención se relaciona con un método para producir una enzima recombinante, más particularmente, esta invención se relaciona con un método para producir la N-aciltransferasa de glicina recombinante enzimáticamente activa soluble en agua (GLYAT (E.G. 2.1.3.13)), que incluye las etapas de proporcionar un anfitrión de expresión adecuado; preparar un vector que incluye un gen para expresar GLYAT en el anfitrión de expresión para formar un plásmido de expresión; transformar el anfitrión con el plásmido de expresión para formar un sistema de expresión; expresar el gen GLYAT en el sistema de expresión; y separar el GLYAT expresado del sistema de expresión.
CO13057288A 2010-08-24 2013-03-22 N-aciltransferasa de glicina terapéutica recombinante CO6700839A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ZA201006021 2010-08-24

Publications (1)

Publication Number Publication Date
CO6700839A2 true CO6700839A2 (es) 2013-06-28

Family

ID=45218771

Family Applications (1)

Application Number Title Priority Date Filing Date
CO13057288A CO6700839A2 (es) 2010-08-24 2013-03-22 N-aciltransferasa de glicina terapéutica recombinante

Country Status (9)

Country Link
US (2) US20130224175A1 (es)
EP (2) EP2609197B1 (es)
JP (2) JP2013538055A (es)
KR (1) KR20130094313A (es)
AU (1) AU2011294798B2 (es)
BR (1) BR112013004283A2 (es)
CO (1) CO6700839A2 (es)
DK (1) DK2609197T3 (es)
WO (1) WO2012025895A2 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130224175A1 (en) 2010-08-24 2013-08-29 North-West University Recombinant therapeutic glycine n-acyltransferase
EP2843043A1 (en) * 2013-08-27 2015-03-04 Evonik Industries AG A method for producing acyl amino acids
WO2016049487A1 (en) * 2014-09-26 2016-03-31 Dow Agrosciences Llc Heterologous expression of glycine n-acyltransferase proteins

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE50108027D1 (de) * 2001-12-04 2005-12-15 Brahms Ag Verwendung der Glycin-N-Acyl-Transferase (GNAT) für die Diagnose von Entzündungserkrankungen und Sepsis
WO2003057897A2 (en) * 2002-01-07 2003-07-17 European Molecular Biology Laboratory Recombinant protein expression
US8119385B2 (en) * 2005-03-04 2012-02-21 Bp Corporation North America Inc. Nucleic acids and proteins and methods for making and using them
US20130224175A1 (en) 2010-08-24 2013-08-29 North-West University Recombinant therapeutic glycine n-acyltransferase
WO2016049487A1 (en) * 2014-09-26 2016-03-31 Dow Agrosciences Llc Heterologous expression of glycine n-acyltransferase proteins

Also Published As

Publication number Publication date
WO2012025895A3 (en) 2012-05-03
AU2011294798A1 (en) 2013-03-14
EP3222713A1 (en) 2017-09-27
US20130224175A1 (en) 2013-08-29
KR20130094313A (ko) 2013-08-23
EP2609197A2 (en) 2013-07-03
JP2017025063A (ja) 2017-02-02
DK2609197T3 (en) 2018-04-23
WO2012025895A2 (en) 2012-03-01
JP6267285B2 (ja) 2018-01-24
AU2011294798B2 (en) 2015-06-11
US10125353B2 (en) 2018-11-13
EP2609197B1 (en) 2018-01-03
JP2013538055A (ja) 2013-10-10
US20170073650A1 (en) 2017-03-16
BR112013004283A2 (pt) 2016-05-31

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