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CN2665715Y - Dot enzyme immunity detecting plate reaction device capable of automatically separating and transporting blood plasma - Google Patents

Dot enzyme immunity detecting plate reaction device capable of automatically separating and transporting blood plasma Download PDF

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Publication number
CN2665715Y
CN2665715Y CN 200320124228 CN200320124228U CN2665715Y CN 2665715 Y CN2665715 Y CN 2665715Y CN 200320124228 CN200320124228 CN 200320124228 CN 200320124228 U CN200320124228 U CN 200320124228U CN 2665715 Y CN2665715 Y CN 2665715Y
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CN
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reaction
plasma
plate
hole
box
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CN 200320124228
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Chinese (zh)
Inventor
周俊华
滕红
邵伟明
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安徽高山药业有限公司
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Abstract

Disclosed is a plate reaction apparatus with automatic separation and transferring for plasma dot-enzyme immunoassay, which is characterized in that the apparatus is composed of a plate shape plasma separator and a hollow reaction box. The center of the plasma separator is the sample-hole which penetrates the plate, and a processing pad is placed in the sample-hole. The reaction box is a hollow box which comprises a cover plate and a base. A penetrating reaction hole with a reaction film stuck on is arranged on one side of the cover plate, while the plasma separator is buttoned in the channel of the cover plate, and the sample hole of the plasma separator covers on the reaction hole. A mobile imbibing pool is provided, which comprises a tank in the reaction box and a handle extending out of the reaction box. The utility model only takes trace peripheral whole blood as the blood sample, automatically finishes the separation and transferring of the plasma, which is simple in operation and without pollution by the waste liquid.

Description

自动分离转运血浆斑点酶免疫检测板块反应装置 Automatic separation of the transport plate plasma reactor dot enzyme immunoassay means

技术领域 FIELD

:本实用新型是用于生物标本检测的装置,特别是用于需微量血液自动血浆分离的斑点酶免疫检测装置。 : The present invention is an apparatus for detecting a biological specimen, in particular the need for micro dot enzyme immunoassay automatic blood plasma separation apparatus.

背景技术 Background technique

:斑点酶免疫检测装置广泛用于人体血液中的蛋白、病毒等的检测,但传统方法多需仪器分离出血浆后才能用于检测,取样量较多,常需静脉取血,不易被患者,特别是儿童患者所接受。 : Dot enzyme immunoassay devices are widely used to detect the protein, etc. of the virus in human blood, but the conventional methods required multiple instruments after separation of the plasma can be used for detecting, large sample volume, venous blood is often required, can not easily be patient, In particular accepted in pediatric patients. 不仅如此,因其检测的灵敏度较低,不适用于较低浓度活性物质的检测。 Moreover, because of the lower sensitivity of detection is not suitable for detecting low concentration of active substance.

发明内容 SUMMARY

:本实用新型所要解决的技术问题是避免上述现有技术所存在的不足之处,提供一种仅以微量末稍全血为血样、自动完成血浆分离转运、操作简便的自动分离转运血浆斑点酶免疫检测板块反应装置。 : Present invention The technical problem to be solved is to avoid the above shortcomings of the prior art is present, only traces provided a peripheral blood sample of whole blood, plasma separation automatically transport, easy to operate the automatic transport of separated plasma spot enzyme immunoassay plate reactor.

本实用新型解决技术问题所采用的技术方案是:本实用新型的结构特点是由分体设置的血浆分离器和反应盒构成;其中:血浆分离器为板块状,中央为贯通其板块的加样孔,在加样孔中放置有处理垫;反应盒为中空的盒状体,由盖板和底座构成,其盖板的一侧区域设有贯通的反应孔,反应孔上粘贴有反应膜,血浆分离器扣设在盖板的卡槽中,其加样孔覆盖在反应孔上;设置移动式吸液池,由位于反应盒中的池体和伸向反应盒外部的手柄构成,吸液池经移动至少可处在两个位置上,其一是池体位于反应孔的正下方,其二是池体完全离开反应孔所在位置。 Aspect of the present invention to solve the technical problem is that: the present invention the structural characteristics of a plasma separator and the reaction cartridge is provided by separate bodies; wherein: plasma separator is a plate-shaped, the central through its plate loading holes, in the loading processing pads is placed in the hole; reaction cassette hollow box-like body, and is composed of a base plate which is provided with a side area of ​​the cover hole penetrating the reaction, the reaction film is stuck wells, plasma separator buckle slot provided in the cover plate, which covers the loaded reaction well bore; provided movable suction sump, the handle is located in the reaction of the cell body and the cartridge toward the cartridge outside the reactor configuration, the absorbent reservoir via the mobile may be in at least two positions, one of which is positioned directly below the reaction well of the cell body, the cell body is completely away from the other location of the wells.

与已有技术相比,本实用新型的有益效果体现在:1、本实用新型是采用垂直转运的方式,其血浆分离、转运以及相关的反应都是在同一位置上完成,检测过程快速简捷。 Compared with the prior art, the present invention is reflected in the beneficial effects: 1, the present invention is a vertical transport way, plasma separation, transport and related reactions are performed in the same position, the detection process is simple and fast.

2、本实用新型只需要微量全血,减少样品使用量可更有利于推广使用。 2, the present invention requires only trace amounts of whole blood, reducing the amount of sample can be more conducive to the promotion of the use.

3、本实用新型中移动式吸液池的设置可使检测过程不需要水洗,不仅减化了操作,而且还有效地避免了废液可能带来的污染。 3, the present invention is provided in the movable liquid suction tank allows detection process does not require water, not only simplify the operation, but also effectively prevent the contamination of the waste may bring.

4、本实用新型整个背景清晰、有利于结果的判读。 4, the entire background of the present invention is clear, is conducive to the interpretation of the results.

5、本实用新型因其检测灵敏度高,也能适用于较低浓度活性物质的检测。 5, the present invention because of its high detection sensitivity, can be adapted to detect a lower concentration of active substance.

附图说明 BRIEF DESCRIPTION

:图1为本实用新型整体外形示意图。 : FIG. 1 is a schematic view of the overall shape of the new utility.

图2为本实用新型血浆分离器结构示意图。 FIG 2 is a schematic perspective view illustrating the structure of a practical plasma separator.

图3为本实用新型反应盒盖板结构示意图。 3 is a schematic perspective view illustrating the cover plate structure useful reaction.

图4为本实用新型吸液池结构示意图。 4 is a schematic perspective view illustrating the liquid-absorbent structure useful pool.

图5为本实用新型反应盒底座结构示意图。 5 is a schematic perspective view illustrating the practical reaction cartridge base structure.

图中标号:1血浆分离器、2反应盒、3吸液池、4加样孔、5处理垫、6盖板、7底座、8反应孔、9反应膜、10卡槽、11池体、12手柄、13抠手。 FIG numeral: plasma separator 1, the reaction cassette 2, the absorbent reservoir 3, 4 sample hole, the processing pad 5, the cover 6, a base 7, 8 wells, reaction film 9, the slot 10, the cell body 11, 12 the handle 13 to pull the hand.

具体实施方式 detailed description

:参见附图1,本实施例是由分体设置的血浆分离器1和反应盒2构成。 : Referring to Figure 1, the present embodiment is a plasma separator embodiment is provided by a separate cartridge 2 and the reactor configuration.

图2所示,血浆分离器1为板块状,中央为贯通其板块的加样孔4,在加样孔4中放置有处理垫5。 As shown in FIG. 2, the plasma separator 1 is a plate-shaped, the center of which a loading through-hole 4 of the plate, placed in the loading aperture 4 has a processing pads 5.

如图3、图5所示,反应盒2为中空的盒状体,由盖板6和底座7构成,其盖板6的一侧区域设有贯通的反应孔8,反应孔8上粘贴有反应膜9,血浆分离器1扣设在盖板的卡槽10中,其加样孔4覆盖在反应孔8上。 3, FIG. 5, a reaction cartridge 2 a hollow box-like body, constituted by the cover plate 6 and the base 7, a side cover which is provided with a reaction zone 6 through holes 8, 8 is attached on wells the reaction film 9, the plasma separator 1 is provided in the buckle slot cover 10, which covers the loading aperture 4 on 8 wells. 具体实施中,反应膜9直接粘贴在上盖板6上,封闭了废液吸取部分,使反应孔周围的背景为同一色,减少视觉影响导致的判读误差,外观整洁。 In particular embodiments, the reaction film 9 is directly attached to the upper cover 6, a closed draw waste portion, so that the surrounding background color for the same reaction wells to reduce the visual impact due to interpretation errors, clean appearance.

如图1、图4所示,本实施例中设置移动式吸液池3,由位于反应盒2中的池体11和伸向反应盒2外部的手柄12构成,吸液池3经移动至少可处在两个位置上,其一是池体11位于反应孔8的正下方,其二是池体11完全离开反应孔8所在位置。 1, FIG. 4, this embodiment is provided movable suction pan 3, the reaction cassette 11 is located outside the reactor 2 and the cartridge toward the handle body 212 cell configuration of the present embodiment illustrated, the absorbent reservoir 3 can move at least by in two positions, one of which is positioned directly below the reaction tank 11 of the hole 8, and the second location 8 is completely out of the cell body 11 wells. 具体使用中,在池体11中应放置具有良好吸附性能的吸附层。 Particular use, the cell body 11 should be placed in the adsorption layer having good adsorption properties.

此外,如图1、图2所示,为了便于使用,在血浆分离器1的边缘上,有一凸出的抠手13。 Further, in FIG. 1, FIG. 2, for ease of use, on the edge of the plasma separator 1, a projection 13 of the hand pull. 为了便于手持,将反应盒2的外形设置为腰形。 To facilitate hand, the reaction profile of the cartridge 2 is set to be waisted. 两端宽,中部呈圆弧形渐窄。 Both ends of the width, the central circular arcuate taper.

操作过程:1、全血样加入加样孔4中,经血浆分离器1中处理垫5的过滤,血浆渗入反应膜9;2、利用抠手13将血浆分离器1从反应盒2的卡槽10中取下,分离出的血浆在反应膜9上进行亲和反应。 Procedure: 1, the whole blood sample Jia Rujia hole 4, through the plasma separator 1 processing filter mat 5, penetrate the plasma membrane reactor 9; 2, 13 using the slot to pull the hand of the plasma separator 1 from the reaction cartridge 2 10 is removed, the plasma was separated and affinity reactions on the reaction membrane 9.

3、由位于反应孔正下方的已放置吸附垫层的池体11中的吸附层对亲和反应的废液进行吸附,拉出池体11。 3, located in the wells have been placed directly below the adsorption layer of the adsorption cell body 11 to cushion the waste adsorption affinity reaction, the cell body 11 is pulled out.

4、向反应孔8中滴入显色剂,并将反应盒2置放在规定温度条件下进行反应,并在规定的时间取出。 4, the hole 8 was added dropwise to the reaction reagent, and the reaction cartridge 2 is placed at a predetermined temperature the reaction conditions and removed at the specified time.

5、推入吸液池3,使其位于反应孔8的正下方,由池体11内放置的吸附层快速吸尽显色剂,酶促反应结束。 5, push the absorbent reservoir 3, to be located immediately below the aperture 8 of the reaction, the absorbent layer 11 of the cell body rapid pull the toner filling, the end of the enzymatic reaction.

Claims (3)

  1. 1.自动分离转运血浆斑点酶免疫检测板块反应装置,其特征是:由分体设置的血浆分离器(1)和反应盒(2)构成;其中:血浆分离器(1)为板块状,中央为贯通其板块的加样孔(4),加样孔(4)中放置有处理垫(5);反应盒(2)为中空的盒状体,由盖板(6)和底座(7)构成,其盖板(6)的一侧区域设有贯通的反应孔(8),反应孔(8)上粘贴有反应膜(9),血浆分离器(1)扣设在盖板的卡槽(10)中,其加样孔(4)覆盖在反应孔(8)上;设置移动式吸液池(3),由位于反应盒(2)中的池体(11)和伸向反应盒(2)外部的手柄(12)构成,吸液池(3)经移动至少可处在两个位置上,其一是池体(11)位于反应孔(8)的正下方,其二是池体(11)完全离开反应孔(8)所在位置。 1. Automatic isolated dot enzyme immunoassay transport plate plasma reaction apparatus, wherein: plasma separator (1) and a reaction cartridge (2) is provided separately from the composition; wherein: plasma separator (1) is plate-shaped, the central with a loading aperture (4), a loading aperture (4) is placed in the processing pad (5) through which plate; reaction cartridge (2) is a hollow box-like body, by a cover plate (6) and the base (7) configuration, which cover (6) is provided with a side area of ​​the reaction opening (8) therethrough, is attached reaction membrane (9) of wells (8), the plasma separator (1) provided in the buckle slot cover (10) in which the loading aperture (4) covers the reaction well (8); provided movable suction pan (3) by positioned the reaction cassette cell body (11) (2) and toward reaction cartridge ( 2) outside the handle (12), and the suction pan (3) can be moved via at least two positions, one of which is just below the cell body (11) located in the wells (8) and the second is the cell body (11) completely away from the location of the wells (8).
  2. 2.根据权利要求1所述的自动分离转运血浆斑点酶免疫检测板块反应装置,其特征是在所述血浆分离器(1)的边缘有一凸出的抠手(13)。 The automatic transport of separated plasma dot enzyme immunoassay plate of the reaction apparatus of claim 1, wherein a projecting hand pull (13) at the edge of the plasma separator (1).
  3. 3.根据权利要求1所述的自动分离转运血浆斑点酶免疫检测板块反应装置,其特征是所述反应盒(2)的外形设置为便于手持的腰形。 The automatic transport of separated plasma dot enzyme immunoassay plate of the reaction apparatus of claim 1, wherein said reaction cartridge (2) to facilitate holding the shape of the waisted.
CN 200320124228 2003-12-31 2003-12-31 Dot enzyme immunity detecting plate reaction device capable of automatically separating and transporting blood plasma CN2665715Y (en)

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CN 200320124228 CN2665715Y (en) 2003-12-31 2003-12-31 Dot enzyme immunity detecting plate reaction device capable of automatically separating and transporting blood plasma

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CN 200320124228 CN2665715Y (en) 2003-12-31 2003-12-31 Dot enzyme immunity detecting plate reaction device capable of automatically separating and transporting blood plasma

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101326442B (en) 2005-12-14 2013-06-05 金伯利-克拉克环球有限公司 Metering strip and method for lateral flow assay devices

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101326442B (en) 2005-12-14 2013-06-05 金伯利-克拉克环球有限公司 Metering strip and method for lateral flow assay devices

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