CN210612952U - Integrated sample extraction box - Google Patents

Abstract

The utility model discloses an integrated sample extraction box, which comprises a collection box, an extraction unit array layer and a top cover clamped with the collection box, wherein a plurality of collection holes arranged in an array are arranged in the collection box; the extraction unit array layer comprises a plurality of extraction units which are arranged corresponding to the collection holes and are arranged in an array mode, a capacity cup for accommodating samples and solvents is arranged at the upper end of each extraction unit, an upper sieve sheet is pressed and filled at the bottom of each capacity cup, and a filling column is arranged below each sieve sheet. The utility model relates to an integration sample extraction box, with sampling, extraction, collection, advance consumptive material integration such as appearance and be a box, the simplified operation degree of difficulty greatly.

Description

Integrated sample extraction box

Technical Field

The utility model relates to an analysis sample pretreatment field especially relates to an integration sample extraction box.

Background

In the sample analysis process, the whole process from pretreatment to sample introduction generally uses consumables such as a sampling tube, a centrifuge tube, an extraction column or an extraction vessel, a collection tube, a sample introduction bottle and the like, and the process is the part with the largest labor cost consumption in sample analysis and detection; the manual material adding consumption is usually the main cost of chemical analysis, and a large amount of disposable material is also the main solid waste source of the current analysis laboratory, thereby consuming resources, increasing the cost and polluting the environment.

The analytical testing of the sample involves multiple steps, and each step involves multiple steps. For example, pharmaceutical analysis of blood samples, the first step is to collect the blood sample, which includes drawing the blood sample from the used organism with a blood collection tube, then centrifuging to remove cells and platelets from the sample and transferring the supernatant plasma to a new sample tube; the second step is to carry out sample pretreatment on the plasma, mainly to remove proteins in the plasma, and if necessary, liquid-liquid extraction or solid-phase extraction is also needed to remove endogenous substances such as nutrients, electrolytes, metabolites and the like which may interfere with analysis and detection in the blood sample. Consumables such as pipette tip, test tube, centrifuging tube all need be used to almost every step in the whole process, still need to handle good sample liquid with the appearance bottle splendid attire of advancing of sealed lid at last. Therefore, for the analysis and detection of drugs in blood samples, a plurality of consumables are needed from blood sampling to on-machine measurement, and the operation is time-consuming, troublesome and error-prone. In fact, most analytical testing works are very similar to the analysis of drugs in blood samples, with more than about 80% of error cases and the consumption of time and consumables occurring during sample processing.

Since biological samples are more complex than other types of samples and the sample size is usually small, only tens of microliters to a few milliliters, the difficulty of sample processing is correspondingly large, and the sample processing is not easy to be remedied once errors occur. At present, the drug detection and analysis in biological samples, especially blood samples, usually uses protein precipitation, liquid-liquid extraction or solid-phase extraction methods to separate and extract the drug molecules, and the specific methods and characteristics are listed as follows:

1. protein precipitation method

① quantitatively absorbing a sample into a centrifuge tube, ② adding an organic reagent, a strong base, a strong acid, a salt and the like to denature and precipitate the protein, vibrating the centrifuge tube to enable the reaction to be sufficient, ③ centrifuging for a few minutes, ④ taking supernatant in the centrifuge tube to transfer to an injection bottle, ⑤ transferring the substance to be detected into a sample tray of an automatic sample injector and then starting detection, processing a batch of 100 samples for about 2-3 hours, wherein the required consumables comprise the centrifuge tube, a pipette tip for twice pipetting, a sample injection bottle and a sealed bottle cap, and the cost is about ￥ 2.3.3 yuan for each sample.

2. Liquid-liquid extraction

In the case that protein precipitation cannot be applied, a liquid-liquid extraction method is generally preferred for pretreatment of biological samples, the liquid-liquid extraction method is to separate substances to be detected through the solubility difference of the substances to be detected in mutually insoluble solvents, the method is easy to implement without special instruments and is relatively cheap, the defects are that a large amount of time and energy must be spent in advance to develop the method, the extraction process is relatively complicated, the operations of liquid transfer multiple steps, oscillation, centrifugation, sample transfer, container marking and the like are still involved, time and labor are consumed, a large amount of organic reagents are required, environmental pollution and cost increase are caused, liquid-liquid extraction generally needs consumable materials such as a liquid transfer gun head, a centrifugal tube, a sample inlet bottle, a sealed bottle cap and the like, the cost of consumable materials (including the solvent) of each sample is about more than ￥ 3 yuan, the processing time of 100 samples is at least 1-2 days, and if the volume of extraction liquid is too large, and concentration is required, the processing time and the cost of.

3. Solid phase extraction process

The method can separate, enrich and purify the samples by selective adsorption, elution and other modes, can effectively reduce matrix interference and improve detection sensitivity, the solid phase extraction needs to be developed in advance, and the method development has higher requirements on professional level and experience, the solid phase extraction operation is more complex and fussy, and each sample of consumable material cost needs ￥ 15-20 yuan or even higher, the time for processing a batch of 100 samples is about 4-16 hours (determined according to the specific form of the solid phase extraction column), the extraction function of the utility model can be regarded as an integrated solid phase extraction device, and representative similar products in the market at present have the following parts:

a solid-phase extraction column similar product (Thermo Scientific SOLA) is characterized in that a sieve plate is not needed on the basis of a traditional solid-phase extraction column, polyethylene sieve plate materials and matrix components are fused into a uniform adsorption column bed, the principle and the use are not obviously different from those of the solid-phase extraction column, but a filtering mode is adopted, the solid-phase extraction column is simple and easy to implement compared with the traditional solid-phase extraction column, the method development is hardly needed, the cost is as high as that of other solid-phase extraction columns, the solid-phase extraction column is only used for sample extraction, the function of collecting and replacing a sample feeding bottle is not provided, a single product is spread to about ￥ 15 yuan per sample, and about ￥ 17 yuan per sample is added with a sample feeding bottle and a cover.

The solid phase extraction column for biological samples, especially blood samples, is rarely used in actual work, mainly because common solid phase extraction products need time-consuming development method, the operation process is complicated, and the cost of each extraction column is over ￥ yuan.

The above solutions all have certain disadvantages, and especially, the sample treatment and sample introduction analysis cannot be integrated to increase the speed and reduce the cost.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, the utility model provides an integrated sample extraction box, which comprises a collection box, an extraction unit array layer which can be partially contained in the collection box, and a top cover which can cover the extraction unit array and can be buckled and sealed with the collection box, wherein a plurality of collection holes which are arranged according to an array are arranged in the collection box; the extraction unit array layer comprises a plurality of extraction units which correspond to the collection holes of the collection box one by one, the upper end of each extraction unit is provided with a capacity cup for receiving a sample, the bottom of the capacity cup is pressed and filled with an upper sieve sheet, a filling column is arranged below the sieve sheet, the filling column is filled with a filling material, and the bottom of the filling column is pressed and filled with a lower sieve sheet and contracted into an outflow port with a tip; the top cover comprises a box cover matched with the collecting box and a built-in sealing gasket, and penetrating holes which are arranged in the same manner as the collecting hole array are formed in the top surface of the box cover.

Further, the upper surface of the upper sieve plate in the extraction unit comprises a hydrophilic polymer material layer.

Furthermore, the depth of the collecting hole is 10-50 mm, and the internal volume is 0.1-2 mL.

Further, the top edge of the collection hole is higher than the top surface of the collection box and forms a closed boundary, and the packing material is linear molecule or reticular molecule and is packed in the packing column along the axial direction in a stretching mode.

Further, the top plane of the extraction unit array layer extends to the periphery and vertically extends downwards to form a skirt wall, and the top plane of the extraction unit array layer and the skirt wall form a sealed box with a downward opening.

Further, the outer contour of the packed column portion of the extraction unit matches the inner contour of the collection well on the collection box, and the outer diameter and height of the packed column of the extraction unit are correspondingly smaller than the inner diameter and depth of the collection well.

Furthermore, the penetrating hole is provided with a hole, and the diameter of the hole of the penetrating hole is approximately the same as the maximum diameter of the collecting hole.

Furthermore, the outer surfaces of the peripheral vertical walls of the collecting box are respectively provided with one or more bayonets, the corresponding positions of the inner surfaces of the peripheral vertical walls of the box cover are respectively provided with one or more buckles, and the bayonets and the buckles are the same in number.

Furthermore, the vertical wall of at least one side of the peripheral vertical walls of the box cover is provided with two notches, and all the buckles of the inner wall are distributed between the two notches.

Has the advantages that: the embodiment of the utility model provides an integration sample extraction box, it is integrated as a box with consumptive material such as sampling, extraction, collection, advance kind, sample analysis from the sampling, handle to advance kind, deposit kind and integrate, simplify the operation degree of difficulty greatly.

Compared with similar products, the utility model discloses an integration sample extraction box need not to shift the collecting fluid or use and advance appearance bottle and bottle lid, works as the utility model discloses a top sieve piece adopts can stabilize the cell the linear hydrophilic material attached to its upper surface's processing after, can directly be used for whole blood to handle in the filling column, and any similar product does not all have the function of handling whole blood sample at present. Therefore, the utility model discloses both remain the function of any current solid phase extraction product, provided the extract again and detected the accessible butt joint of used automatic sample injector with the analysis, saved the transfer of collecting fluid and advance appearance bottle and sealed lid to furthest has portably extracted operation and consumptive material expense.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention. Wherein:

fig. 1 is a cross-sectional view of an integrated sample extraction cartridge according to the present invention;

FIG. 2 is a cross-sectional view of the sample extraction cartridge disassembled;

FIG. 3 is a schematic view of the top cover being snapped into the collection cassette after sample processing is complete to change into a sample entry cassette;

FIG. 4 is a schematic diagram of the arrangement of the top cover through holes, the extraction units and the collection hole arrays;

figure 5 is a schematic of evacuation filtration.

Labeled as:

10-a top cover; 11-a through hole; 12-buckling; 13-built-in sealing gasket;

20-extraction unit array layer; 21-an extraction unit; 22-extraction unit packed column; 23, screening sheets;

24-lower sieve sheet; 25-skirt wall;

30-a collection box; 31-a collection well; 32-bayonet;

40-array.

50-evacuation of the filtration device or vacuum chamber.

Detailed Description

The technical solution of the present invention will be further described with reference to the following specific embodiments and the accompanying drawings, and it should be noted that the described embodiments are only used for explaining the present invention, and are not used for limiting the present invention.

With reference to fig. 1 and fig. 2, an embodiment of the present invention provides an integrated sample extraction cartridge, which includes a collection cartridge 30, an extraction cell array layer 20, and a top cover 10.

Specifically, the material of the collecting box 30 is selected from materials that are resistant to chemical reagents, organic solvents, strong acids and bases, and have no adsorption effect on organic matters, such as polypropylene, polyethylene, polytetrafluoroethylene, or glass. And the collection box 30 is sized to fit a standard 96 well plate, in the form of a rectangular plate 127x85mm long by width, to fit a conventional liquid chromatograph injector. In the middle of the collection cassette 30 is an array 40 (shown in fig. 4) of a plurality of collection wells 31 for collecting the filtrate, the array of collection wells may be in the form of 8 rows and 12 columns, or 6 rows and 9 columns, or 6 rows and 8 columns, and the array of collection wells 31 may be arbitrarily selected to match the autosampler. Each collecting hole 31 has a depth of 10 to 50mm and an internal volume of 0.1 to 2 mL.

The terms "plurality" and "a plurality" described in the specification of the present application mean more than one, i.e., 2 or more.

If there is no explicit quantitative limitation on a structure or component in the specification of the present application, it means that the number of the structure or component is not limited, that is, may be one or more.

As shown in FIG. 2, the top edge of the collection hole 31 is 0.5-3 mm higher than the top surface of the collection box 30 to form a closed boundary, so that the collected sample liquid does not overflow into the adjacent collection hole to cause cross contamination, and the protruded edge has better sealing effect after pressing the sealing pad.

The extraction unit array layer 20 is also made of a material that is resistant to chemical reagents, organic solvents, strong acids and bases, and does not adsorb organic substances, such as polypropylene, polyethylene, polytetrafluoroethylene, glass, and the like. The material of the array layer 20 may be the same as or different from that of the collection box 30. The extraction unit array layer 20 is arranged in a form completely corresponding to the collection hole array of the collection box 30, i.e. in a one-to-one correspondence, and the extraction units 21 and the collection holes 31 on the collection box 30 form a mutually matched structure, i.e. the external contour of the packing columns 23 of the extraction units 21 is consistent with the internal contour of the collection holes 31, but the size of the extraction units 21 is slightly smaller. The collection well 31 is a microtube, preferably having a pointed bottom, as shown in FIG. 2. The top of each extraction unit 21 is provided with a cup for receiving a sample and a solvent, the cup is opened upwards, the lower part of the cup is connected with a filling column 22 to form a whole, the top of the filling column at the bottom of the cup is pressed and filled with an upper sieve sheet 23 for preventing a filling material in the filling column 22 from being poured out when being inverted, the upper surface of the upper sieve sheet 23 can be attached with a layer of hydrophilic high polymer material capable of stabilizing cells, when a whole blood sample is added to the surface of the layer, the whole blood sample can be rapidly distributed, the cells in the blood sample are protected by the layer of hydrophilic polymer and are not damaged by a subsequently added organic reagent, and therefore the cup of the extraction unit can be directly used as a collection container for blood sample collection, and the blood collection container and the centrifugal separation step are saved. The packing material filled in the packing column 22 can be any existing solid phase extraction material, and the optimum packing material is natural or artificial synthetic material with linear or reticular molecules and the material after chemical modification treatment, the stretching state of the material molecules is kept as much as possible during the filling, namely, the linear molecules are filled along the axial direction of the packing column 22 and the reticular molecules are filled along the axial direction of the packing column 22 in a flat rolling mat shape, the bottom of the packing column 22 is contracted into a pointed outflow port so as to facilitate the filtered liquid (sample to be detected) to flow into the collecting hole 31, and a lower sieve sheet 24 is pressed and filled inside the contracted outflow port and used for preventing the packing material and the precipitated substances in the sample from entering the collecting hole 31. The tops of all the extraction units 21 are separated from each other and connected with each other to form a closed surface (the tops of the extraction units 21 are in the same plane during molding).

In addition, the outer diameter and height of the packed column part 22 of the extraction unit 21 are slightly smaller than the inner diameter and depth of the collection hole 31 on the collection box, so that all or part of the packed column part can be matched with the collection hole 31 on the collection box, the extraction unit array layers are superposed on the collection box during packaging and transportation, and the packed column 22 of each extraction unit 21 can be just inserted into the corresponding collection hole 32 on the collection box, so that the package is stable and compact.

In order to facilitate the sealing of the extraction unit array layer 20, the edge part of a closed surface (which is adhered, and forms a sealing surface without being blocked or hollowed out, i.e. a top plane) formed by the links between the extraction units 21 in the extraction unit array layer 20 extends 3-20 mm all around and vertically extends 2-20mm downwards to form a skirt wall 25, the thickness of the skirt wall 25 is preferably 1-3mm, and the lower edge of the skirt wall 25 is smooth, when the extraction unit array layer 20 is placed on a suction filtration device with a connector size matched with the contour of the extraction unit array layer 20, the closed surface of the extraction unit array layer 20, the skirt wall 25 and the sealing ring of the suction filtration connector can form a sealed vacuum suction filtration cavity, and each extraction unit 21 and each suction filtration cavity do not need to be independently butted and respectively sealed, so that the design of the suction filtration device 50 is simplified (as shown in fig. 5), and the butt joint of the extraction unit array layer 20 and the vacuum extraction device 50 is realized through the smooth and narrow skirt wall 25 The edge is in abutting contact with the sealing ring, the length of a sealing line is greatly reduced, and the sealing effect and the stability can be greatly improved.

Specifically, the top cover 10 is a rectangular box cover with a sheet of sealing gasket 13 inside, the box cover 11 can be made of plastic, wood, metal or glass, the top surface of the box cover is an array 40 of penetrating holes 11, the arrangement of the penetrating holes 11 is consistent with the arrangement of the collecting holes 31 on the collecting box 30, and the hole diameter of each penetrating hole 11 is the same as or slightly smaller than, preferably the same as, the maximum diameter of the corresponding collecting hole 31 on the collecting box. If the hole is too small, the sample injector needle is easy to be inclined so as to be mistakenly inserted into the edge of the hole to cause the damage of the needle, and meanwhile, the sealing gasket 13 is arranged on the inner surface of the top of the box cover.

In order to facilitate the relative fixing of the collection box 30 and the top cover 10, one or more bayonets 32 are respectively disposed on the outer surfaces of the peripheral vertical walls of the collection box 30, and one or more hooks 12 are respectively disposed on the inner surfaces of the peripheral vertical walls of the box cover 11 to correspond to the bayonets 32, as shown in fig. 2. The number and the position of the buckles 12 correspond to the bayonets 32 on the collecting box one by one. This arrangement allows the lid with the gasket 14 to be pressed onto the collection container 31 to form a tight fit so that each collection well 31 is tightly sealed.

On the other hand, the sealing gasket 13 is arranged between the inner surface of the top surface of the box cover 10 and the buckle 12 and is limited by the buckle 12 to avoid falling from the top cover, after the top cover is pressed on the collecting box, each buckle 12 is just buckled in the bayonet 32, so that the sealing gasket 13 in the top cover 10 tightly seals each collecting hole 31, the top cover 10 and the collecting box 30 are combined to form a sample feeding box which is firm in combination, and the sample feeding box can be directly placed in a sample feeding disc of an automatic sample feeder to perform sample injection and analysis detection. However, since the number of samples is not always just used up for a whole extraction box, the extraction unit which is not used up can be stored for the next time, which requires that the pressed sample box can be conveniently opened to collect the extraction liquid again and then be pressed and buckled back to form a sealed sample box.

The thickness of the sealing gasket 13 is preferably 1-2mm, and the utility model adopts 1.6 mm. If the thickness is too thin, the compaction degree is not tight easily, and the sealing is difficult to ensure; too thick may cause the needle to be unable to pierce the gasket, resulting in damage to the needle. The gasket 13 is typically constructed of a flexible cushion such as silicone, rubber, etc. plus a dense film such as polypropylene, tetrafluoroethylene, polyethylene, etc. The elastic soft cushion is used for ensuring the sealing property after being compressed, has a certain self-healing function, and can recover the sealing property after the sample injection needle is pricked so as to prevent the solution from volatilizing; the dense film layer faces downwards to collect solution, and the dense film layer mainly prevents the sealing gasket from interacting with the collected solution by chemical reaction, adsorption, dissolution and the like, so that interference is avoided. The densified layer may be omitted if the material used for the resilient cushion layer is itself sufficiently inert.

The embodiment of the utility model provides an optimal mode is that the adoption can have strong adsorption to the sample matrix and load the extraction post to the less filler of the material adsorption that awaits measuring to the interference that the filtration mode removed the sample matrix and let the determinand be washed out the extraction unit by the eluting solution, obtain with the solid phase extraction the same effect of extracting the purification to the sample, furthest improves the speed and the efficiency of sample processing from this. However, the utility model discloses also can adopt solid phase extraction filler commonly used to fill the extraction column, reach the purpose of sample extraction purification through solid phase extraction step commonly used, will collect the box and aim at the extraction column array when final step is collected, receive final eluant and be used for advancing kind or further concentration and redissolution processing.

To the processing of blood specimen, body fluid or water sample, the utility model discloses the filler that uses is the linear or netted material that has very strong adsorption to hydrone and strong hydrophilic molecule, guarantees as far as possible during the packing to fill in the extraction post with the form of stretching in, the hydrone in the sample that awaits measuring forms the water film of one deck continuous adsorption along the filler molecule, the molecule that water solubility is strong then both distributes in the water film and is adsorbed by the filler of linear setting again simultaneously in the sample to difficult quilt so can the maximum detention by the eluant elution; organic molecules to be analyzed, such as chemical or agricultural residual molecules, are generally hydrophobic to some extent, and therefore are not easily adsorbed by the filler and are more easily distributed into the eluent to be eluted into the collection liquid.

In the embodiment for pretreatment of a whole blood sample, the upper surface of the upper sieve sheet 23 in the extraction unit 21 contains a layer of hydrophilic polymer material having a protective effect on cells, so that the product can be directly used for whole blood treatment, and after the whole blood sample is applied thereto, the protective material can prevent the cells in the blood from being destroyed by a subsequently added reagent or solvent, thereby preventing intracellular substances from entering the final analysis sample to interfere with the whole blood treatment.

The embodiment of the utility model provides an extraction box is preferred to be applicable to the medicine in detecting the organism and other samples that await measuring.

From collection, processing, until the analysis of operating the computer, whole process is except using moving liquid and suction filtration, and all experiment consumptive materials all include the utility model discloses within the above-mentioned embodiment, need not to use other consumptive materials, consequently, the utility model discloses an extraction box can greatly limit simplified operation process, reduce consumptive material quantity, make things convenient for waste recovery. The utility model discloses a top cap and collection box both had replaced the sealed lid of the higher collecting pipe of expense, sample bottle and sample bottle again in sample processing as the packaging material of extraction unit so that the transportation and the storage of product. The sample injection box (as shown in figure 3) formed by pressing and buckling the top cover and the collection box adopts the whole sealing gasket, so that the sample injection needle cannot deform after penetrating and injecting samples, and the self-healing recovery sealing capability is improved greatly compared with the sealing gasket of a single sample injection bottle cover. Experimental result shows, still can guarantee after the injection needle pierces through same position 8 times in succession the utility model discloses the extraction box of embodiment can not have the solution to ooze after inverting.

The embodiment of the utility model provides an extraction box can be used for the suction filtration, also can be used for the pressure differential of other modes to filter, if filter the top pressurization on extraction unit layer.

Furthermore, the embodiment of the utility model provides a sample introduction box (extraction box) can be from top to bottom stable overlapping, and is particularly convenient to the sample storage, and a middle-size and small-size refrigerator just can stack and store ten thousand samples. The sample injection box has the same external dimension with an international standard 96-well plate, is matched with all common automatic sample injectors and is also matched with common automated liquid treatment equipment in a laboratory without obstacles.

According to another embodiment of the present invention, the integrated sample extraction cartridge is used as follows:

1. placing the collecting box 30 at the bottom of the suction filtration device 50, and implementing and positioning;

2. placing the extraction unit array layer 20 into a butt joint window of a vacuum filtration device 50, and compacting;

3. adding samples one by one to the upper sieve sheet 23 in the extraction unit 21 of the extraction unit array layer 20, wherein the volume of the added samples depends on the capacity of a single extraction unit and is usually between 5 mu L and 5 mL;

4. adding a washing solution into each extraction unit 21, wherein the components of the solution and the adding volume are determined by specific substances to be separated and the characteristics of the extraction unit, and the adding volume is 50 mu L-10 mL generally;

5. starting suction filtration, leading the sample and the flushing solution to undergo the processes of mixing, adsorption, distribution, elution and the like in the extraction unit, and finally collecting the solution of the object to be detected eluted from the extraction unit into the collection hole 31;

6. until all the extraction units are pumped to be dry, after all the solution enters the collecting hole 31 below, the suction filtration is closed, and the air escape valve is opened to slowly eliminate the pressure difference;

7. taking the extraction unit array layer 20 down from the suction filtration device, and putting the extraction unit array layer into a waste material for recycling;

8. and opening the vacuum cavity, taking out the collection box 30, pressing the top cover 10 onto the collection box 30, buckling the buckles 12 into the bayonets 32 on the four walls of the periphery of the collection box, buckling the buckles with the bayonets, and directly placing the clamped sample injection box into an automatic sample injector for machine detection.

9. If the collection box needs to be reused, the vertical side wall of the top cover with the notch can be opened by fingers, the top cover can be separated from the collection box, the collection box is placed into the evacuating device 50, the steps 1-8 are repeated, and the units left unused by the extraction units can be gradually used up.

The technical solution of the present invention is further explained with reference to the following embodiments.

Example 1

Use the embodiment of the utility model provides an extract box does the extraction of medicine molecule in the blood sample, can directly add the whole blood sample to the extraction unit, then add protein precipitant and eluant, collect the filtrate after the suction filtration and can be used for the analysis and detection. The protein precipitant is usually methanol, acetonitrile, perchloric acid, or strong acid and strong alkali solution, and the eluting solution can be common organic solvent such as methanol, acetonitrile, acetic acid solution, dimethyl sulfur sulfone, ethyl acetate, chloroform, carbon tetrachloride, etc. Red blood cells, white blood cells, platelets and the like in a whole blood sample can be blocked by a top sieve plate and a filler in a filled column, protein in the blood is changed into solid precipitate after contacting a precipitator and is blocked by the filler and a sieve plate at the lower end of the filled column, endogenous substances in the blood sample, such as phospholipid, blood sugar, blood fat, polypeptide, electrolyte and the like, can be adsorbed by the filler, only drug molecules can be eluted from the filled column by an elution solution and enter a collection box, and the obtained collection liquid is used for LC/MS/MS quantitative analysis. Compared with the common protein precipitation method, the whole process saves more than 90% of the time for processing blood samples, and finally the extracted samples are stored in a sealed sample injection box and can be repeatedly used for analysis sample injection and are convenient to stack, and about 1500 multi-disc sample injection boxes, nearly 15,000 samples, can be placed in a 0.5 cubic meter small and medium-sized refrigerator. If the filler is selected properly, the solution after treatment basically eliminates the interfering substances which can generate matrix effect in the blood sample, so the blood sample after treatment can be subjected to mass spectrum quantitative analysis through direct injection, and the utility model can be made into a 96-hole or even 384-hole array form, thereby supporting a high-throughput quantitative analysis system to the maximum extent.

Example 2

Use the embodiment of the utility model provides an extraction box handles environmental water sample, only needs to add extraction unit with 1 ~ 5mL sample on, direct suction filtration. Each extraction column can be filled with fillers with strong water absorbability, such as polyethylene glycol, polyethylene acetamide, cotton fiber, modified plant fiber and the like, water molecules and hydrophilic substances in a sample, such as humic acid, saccharides and the like, are adsorbed on the fillers, solid substances in a water sample are blocked by an upper sieve plate and the fillers of the filled columns, molecules to be detected with poor absorbability are washed out of the extraction unit by an eluent and enter a collection box, so that most of water and interferents in the water are removed from the collected water sample, the substances to be detected are enriched, and the water sample treatment is completed in one step.

The current method for treating the environmental water sample generally adopts liquid-liquid extraction, wherein the extraction generally adopts more than 500mL of liquid-liquid extraction, a separating funnel is added, an organic solvent which is not miscible with water is added, the mixture is shaken for 20 minutes to several hours, then the mixture is kept stand for layering, the organic phase is separated out and evaporated to dryness, dry residues obtained by evaporation to dryness are preferably dissolved by a small amount of organic solvent, and the obtained solution is used for on-machine analysis. Compare the utility model discloses the one step of sample treatment who targets in place, liquid-liquid extraction or solid phase extraction, use the utility model discloses than liquid-liquid extraction or solid phase extraction, save time more than 95%. Meanwhile, the sample adding amount is greatly reduced, and the detection sensitivity is unchanged.

Taking sample pretreatment for detecting sulfonamide antibiotics in water as an example, the conventional national standard method comprises the steps of taking 1.00L of a water sample from ①, placing the water sample in a separating funnel, adding ② an internal standard substance in the separating funnel, uniformly mixing, adjusting the pH of the water sample to 5.0 by using 50% sulfuric acid solution or 10M sodium hydroxide solution at ②, adding 60mL of dichloromethane into the separating funnel filled with the water sample at ④, covering the separating funnel with ⑤, fully oscillating, periodically releasing gas to release excessive pressure, standing ⑥ for 10min to separate an organic phase from a water phase, collecting a solvent extract from ⑦ in a conical flask, repeating the step 7- ⑦ of ⑧ to extract twice, combining 3 times of extract liquid, carrying out rotary evaporation on ② to 5-10 mL and then blowing nitrogen to dry residues, dissolving the dry residues with 5.00mL of methanol at ② and filtering to obtain a solution to be detected, taking 4 hours in the whole process, taking a water sample with the same sensitivity of sulfonamide antibiotics of about 0.1ppm, taking a 5.00mL column, and carrying out suction filtration to obtain a water sample to be directly used for detecting water absorption of the antibiotics, and obtaining a water absorption of a filler after adding 3.5. 3. 0. 7375. mu.5. 0. about. 0. mu.5. M of the water sample to be directly added to be absorbed in the water sample after the water absorption column to be detected in the water sample to be detected.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims (9)

1. An integrated sample extraction box, which comprises a collection box, an extraction unit array layer which can be partially contained in the collection box, and a top cover which can cover the extraction unit array and can be buckled and sealed with the collection box,

a plurality of collecting holes which are arranged in an array are arranged in the collecting box;

the extraction unit array layer comprises a plurality of extraction units which correspond to the collection holes of the collection box one by one, the upper end of each extraction unit is provided with a capacity cup for receiving a sample, the bottom of the capacity cup is pressed and filled with an upper sieve sheet, a filling column is arranged below the sieve sheet, the filling column is filled with a filling material, and the bottom of the filling column is pressed and filled with a lower sieve sheet and contracted into an outflow port with a tip;

the top cover comprises a box cover matched with the collecting box and a built-in sealing gasket, and penetrating holes which are arranged in the same manner as the collecting hole array are formed in the top surface of the box cover.

2. The integrated sample extraction cartridge of claim 1, wherein the upper surface of the upper screen plate in the extraction unit comprises a layer of hydrophilic polymer material.

3. The integrated sample extraction cartridge of claim 1, wherein the collection well has a depth of 10 to 50mm and an internal volume of 0.1 to 2 mL.

4. The integrated sample extraction cartridge of claim 1, wherein the top edge of the collection well is raised above the top surface of the collection cartridge and forms a closed boundary, and the packing material is linear or reticular and is packed in the packing column in a stretched manner in the axial direction.

5. The integrated sample extraction cartridge of claim 1, wherein the top plane of the extraction cell array layer extends peripherally and vertically downward to form a skirt wall, and the top plane of the extraction cell array layer and the skirt wall form a sealed cartridge with a downward opening.

6. The integrated sample extraction cartridge of claim 1, wherein the outer profile of the packed column portion of the extraction cell matches the inner profile of the collection well on the collection cartridge, and the outer diameter and height of the packed column of the extraction cell are correspondingly smaller than the inner diameter and depth of the collection well.

7. The integrated sample extraction cartridge of claim 1, wherein the penetration hole has a hole therein, and the diameter of the hole is substantially the same as the maximum diameter of the collection hole.

8. The integrated sample extraction cartridge as claimed in claim 1, wherein the outer surfaces of the peripheral vertical walls of the collection cartridge are respectively provided with one or more bayonets, and the corresponding positions of the inner surfaces of the peripheral vertical walls of the cartridge cover are respectively provided with one or more fasteners, and the bayonets and the fasteners are the same in number.

9. The integrated sample extraction cartridge of claim 8, wherein the lid has two notches in at least one of the vertical walls of the periphery of the lid, and all the locking tabs of the inner wall are disposed between the two notches.

Images