CN206683922U - A kind of tubular type magnetic bead separating device - Google Patents
A kind of tubular type magnetic bead separating device Download PDFInfo
- Publication number
- CN206683922U CN206683922U CN201720977512.2U CN201720977512U CN206683922U CN 206683922 U CN206683922 U CN 206683922U CN 201720977512 U CN201720977512 U CN 201720977512U CN 206683922 U CN206683922 U CN 206683922U
- Authority
- CN
- China
- Prior art keywords
- test tube
- pedestal
- magnetic bead
- lower shoe
- cover plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a kind of tubular type magnetic bead separating device, including upper cover plate, lower shoe, the pedestal between upper cover plate and lower shoe, the top of the pedestal to be connected with the upper cover plate, and the bottom of the pedestal is connected with the lower shoe；The upper cover plate is provided with 2 test tube holes placed for test tube, and test tube is fixed jointly with lower shoe for the test tube hole, and 2 test tube holes are respectively positioned at the both sides of the pedestal and close to the pedestal, so that the adjacent pedestal of test tube placed；The pedestal is provided with magnetic bar, and magnetic bead can be adsorbed in test tube medial surface by the magnetic bar.The utility model is simple in construction; cost is cheap; it is easy to carry; it is not easy to wear; it is practical flexible; the large-scale production of the reagent containing magnetic bead in magnetic microparticle chemiluminescence detection kit can be met, can also be widely used in the numerous areas such as bio-separation, drug screening, environment measuring, reach the purpose of separation, enrichment, purification.
Description
Technical field
Separator technical field is the utility model is related to, more particularly, to a kind of tubular type magnetic bead separating device.
Background technology
Immuno magnetic cell separation technology is the nanometer or micron order magnetic bead for having antigen or antibody using surface coupling as separation Jie
Matter, reacted by the high specific between antigen-antibody and capture target substance, then the effect by externally-applied magnetic field, promote object
Matter separates with the other materials in mixture, so as to reach the purpose of separation, enrichment, purifying.The technology has simple and easy, anti-
Short, the specific high, many merits such as loss is small, are widely used to medical diagnosis, bio-separation, drug screening, ring between seasonable
The numerous areas such as border detection.
Magnetic microparticle chemiluminescence immune assay is by magnetic separation technique, chemiluminescence and immuno analytical method three
A kind of emerging analysis method combined.The technology takes full advantage of the high specific of immunoassay, chemiluminescence
The quick easily automation property of high sensitivity and magnetic separation technique, irreplaceable work is presented in biomedical diagnostic field
With.
In magnetic microparticle chemiluminescence immune assay detection kit, the magnetic particle of antigen or antibody is coupled containing surface
Reagent be one of essential constituent.When producing the reagent, it usually needs cleaned by multiple Beads enrichment
Step, in the process, it is necessary to magnetic bead is adsorbed in the tube wall of glass tube using Beads enrichment device, then with pipettor by test tube
Solution take out.
At present, the additional magnet of most Beads enrichment device is usually located at the bottom of test tube, during liquid relief, because magnetic bead gathers
The positive bottom of test tube is combined in, to avoid magnetically attractive pearl by mistake, pipettor generally can not be extended to the bottommost of test tube, consequently, it is possible to nothing
Method suctions out invisible spectro solution completely.Moreover, commercially available Beads enrichment device is mostly small-sized or micro-pore plate type, suitable volume are
Below 10ml plastic test tubes use, and a small number of products are also only capable of operating the plastic centrifuge tube that specification is 15ml or 50ml.But
In the large-scale production of magnetic microparticle chemiluminescence immune assay detection kit, the coupling reaction of antigen or antibody and magnetic bead and
Related separation cleaning step would generally need to be extended to the various glass round bottom pipes that volume is 10 ~ 100ml according to actual order
Middle progress, commercially available prod are difficult to the requirement for reaching large-scale production really.
Utility model content
The utility model is the defects of overcoming described in above-mentioned prior art, there is provided a kind of tubular type magnetic bead separating device.
In order to solve the above technical problems, the technical solution adopted in the utility model is：
A kind of tubular type magnetic bead separating device, including upper cover plate, lower shoe, the pedestal between upper cover plate and lower shoe,
The top of the pedestal is connected with the upper cover plate, and the bottom of the pedestal is connected with the lower shoe；The upper cover plate is provided with 2
Test tube is fixed jointly with lower shoe for the individual test tube hole placed for test tube, the test tube hole, and 2 test tube holes distinguish position
In the both sides of the pedestal and close to the pedestal, so that the adjacent pedestal of test tube placed；The middle part of the pedestal is provided with
The magnetic bar of magnetic bead in test tube can be adsorbed.
The magnetic bar being provided with the tubular type magnetic bead separating device can well by magnetic bead be adsorbed in test tube medial surface and
It is not bottom, such pipettor suctions out solution with regard to that can put in test tube bottom completely so that separating effect obtains tremendous increase.Together
When, a tubular type magnetic bead separating device has two test tube holes, and during production, a laboratory technician can operate two reaction tubes simultaneously,
Operating efficiency is substantially increased, is saved labour turnover.
Preferably, the upper cover plate, lower shoe and pedestal are made up of PMMA materials.Preferably, but it is not limited to, the pedestal
Size be long 8.0cm × wide 5.5cm × high 4.5cm；The size of the upper cover plate is long 18.5cm × wide 8.0cm × thickness
0.5cm；The size of the lower shoe is long 18.5cm × wide 8.0cm × thick 0.5cm.
Preferably, the magnetic bar is cylindrical shape.The both ends of the magnetic bar respectively with the test tube phase positioned at pedestal both sides
It is right.Further, the magnetic bar both ends are exposed in air.Preferably, but it is not limited to, the size of the magnetic bar is diameter
2.0cm, long 7.5cm.The magnetic bar is alnico magnets.
Preferably, the lower shoe is provided with cylindric neck, and the cylindric neck can hold test tube, and with the examination
Test tube is fixed jointly for pore.It is highly preferred that the cylindric neck is hollow cylindrical neck.It is further preferred that
The cylindric neck protrudes from lower shoe.Yet further, the center of circle of the cylindric neck is located in the test tube hole
The underface of the heart.The high 0.5cm of cylindric neck, hollow, inner circle a diameter of 3cm, cylindrical 0.4cm wide between inner circle.
Cylindric neck on the lower shoe, particularly hollow cylindrical neck, sharp bottom or round bottom can be fixed well
The bottom of test tube, and there is anti-skidding effect, the glass round bottom tube manipulation larger to volume is particularly convenient.The hole of upper cover plate and circle
The specification of tubular neck can need to design according to practical application, then coordinate the test tube of variously-shaped specification to use, flexibly and easily.
It is highly preferred that the contact portion of the cylindric neck and test tube be diameter be gradually reduced from top to bottom it is stepped
Structure, to hold different size of test tube.
Preferably, the tubular type magnetic bead separating device supports the use with test tube, and the volume of the test tube is 10 ~ 100mL.
A kind of tubular type Beads enrichment device suitable for volume for 10 ~ 100ml is provided, reaction tube can be sharp bottom or circle
Bottom, the material of test tube can solve most of commercially available prod at present well and are only capable of being below 10ml to volume with plastics or glass
The limitation that is operated of plastic test tube.Be particularly suitable for use in the reagent containing magnetic bead in magnetic microparticle chemiluminescence detection kit
Large-scale production, can also be widely used in the numerous areas such as bio-separation, drug screening, environment measuring, reach separation, rich
The purpose of collection, purification.
Compared with prior art, the beneficial effects of the utility model are：
Magnetic bar is arranged in the pedestal of tubular type magnetic bead separating device, magnetic bead can be adsorbed in the inner side of test tube well
Face rather than bottom, so, pipettor suction out solution with regard to that can put in test tube bottom completely so that separating effect obtains huge carry
Rise.
Further, the lower shoe of the magnetic bead separating device is provided with raised cylindric neck, can fix well
Sharp bottom or the bottom of round bottom test tube, and there is anti-skidding effect.
The utility model is simple in construction, and cost is cheap, easy to carry, not easy to wear, practical flexible；Magnetic particle can be met
The large-scale production of reagent containing magnetic bead in chemiluminescence detection kit, can also be widely used in bio-separation, drug sieve
The numerous areas such as choosing, environment measuring, reaches the purpose of separation, enrichment, purification.
Brief description of the drawings
Fig. 1 is the structural representation of the tubular type magnetic bead separating device of embodiment 1.
Fig. 2 is the structural representation of the tubular type magnetic bead separating device of embodiment 2.
Fig. 3 is the structural representation of the cylindric neck of the tubular type magnetic bead separating device of embodiment 3.
Embodiment
The utility model is further described with reference to embodiment.
Embodiment 1
Fig. 1 is a kind of structural representation of tubular type magnetic bead separating device of the present utility model.A kind of as shown in figure 1, tubular type
Magnetic bead separating device, including pedestal 1, upper cover plate 3 and lower shoe 4, the top of pedestal 1 are connected with upper cover plate 3, and the bottom of pedestal 1 is with
Bottom plate 4 connects, and upper cover plate 3 is provided with two test tube holes 5, and test tube hole 5 is located at is provided with magnetic bar 2 close to the both sides of pedestal 1, pedestal 1,
Magnetic bar 2 is located at the middle part of pedestal 1, and when test tube hole 5 is put into test tube, the both ends of magnetic bar 2 are placed with two test tube holes 5 respectively
Test tube be in contact, the magnetic bead in test tube can be adsorbed in test tube medial surface by magnetic bar 2.
In use, it is respectively charged into Magnetic Isolation reagent and biological sample to be coupled in glass round bottom heavy wall pressure bottle
（Antigen/antibody etc.）, glass round bottom heavy wall pressure bottle shape can be put into the magnetic bead separating device that size matches similar to test tube
In, when Magnetic Isolation reagent and after coupling target substance fully reacts, target substance is by by the magnetic bead in Magnetic Isolation reagent
Absorption, heavy wall pressure bottle is inserted in the test tube hole 5 of magnetic bead separating device, 5min is stood, due to attraction of the magnetic bar to magnetic bead
Effect, it is coated with the medial surface that the magnetic bead of target substance will be adsorbed on heavy wall pressure bottle and be close to bar magnet end.Treat that magnetic bead is complete
After absorption, the bottom that pipettor is carefully goed deep into vial is completely inhaled supernatant and abandoned, and without worrying to remove supernatant drawing
Magnetic bead can be drawn during liquid, can so realize efficiently separating for magnetic bead and solution.The test tube hole of left and right two can facilitate an experiment
Member operates to two vials simultaneously.
Embodiment 2
Fig. 2 is the structural representation of another embodiment of the present utility model.As shown in Fig. 2 including pedestal 1, the He of upper cover plate 3
Lower shoe 4, the top of pedestal 1 are connected with upper cover plate 3, and the bottom of pedestal 1 is connected with lower shoe 4, and upper cover plate 3 is provided with two test tube holes 5,
Test tube hole 5 is located at is provided with magnetic bar 2 close to the both sides of pedestal 1, pedestal 1, and magnetic bar 2 is located at the middle part of pedestal 1, when test tube hole 5
When being put into test tube, the test tube that the both ends of magnetic bar 2 are placed with two test tube holes 5 respectively is in contact, and magnetic bar 2 can be by test tube
Magnetic bead be adsorbed in test tube medial surface；Lower shoe 4 is provided with two cylindric necks 6, and cylindric neck 6 is hollow cylindrical card
Groove, cylindric neck 6 protrudes from lower shoe, and the center of circle of cylindric neck is located at the underface at the center of test tube hole 5.
Hollow cylindrical neck can fix the bottom at sharp bottom or round bottom test tube well, and have anti-skidding effect, to volume
Larger glass round bottom tube manipulation is particularly convenient.The specification of the hole of upper cover plate and cylindric neck can be according to practical application needs
Design, then coordinate the test tube of variously-shaped specification to use, flexibly and easily.
Embodiment 3
The present embodiment is 3rd embodiment of the present utility model, as different from Example 2, cylindric neck 6 and test tube
Contact portion be the step structure that is gradually reduced from top to bottom of diameter, cylindric neck 6 can hold different size of examination
Pipe.Fig. 3 is the structural representation of the cylindric neck 6 of the present embodiment.
Other parts and annexation are same as Example 2.
Obviously, above-described embodiment of the present utility model is only intended to clearly illustrate the utility model example, and
It is not the restriction to embodiment of the present utility model.For those of ordinary skill in the field, in described above
On the basis of can also make other changes in different forms.There is no need and unable to give all embodiments
It is exhaustive.All made within spirit of the present utility model and principle all any modification, equivalent and improvement etc., should be included in
Within the protection domain of the utility model claims.
Claims (5)
1. a kind of tubular type magnetic bead separating device, it is characterised in that including upper cover plate（3）, lower shoe（4）, positioned at upper cover plate（3）With
Lower shoe（4）Between pedestal（1）, the pedestal（1）Top and the upper cover plate（3）Connection, the pedestal（1）Bottom
With the lower shoe（4）Connection；The upper cover plate（3）Provided with 2 test tube holes placed for test tube（5）, the test tube hole（5）With
Lower shoe（4）Test tube is fixed jointly, 2 test tube holes（5）It is located at the pedestal respectively（1）Both sides and close to institute
Pedestal is stated, so that the adjacent pedestal of test tube placed（1）；The pedestal（1）Middle part be provided with can adsorb magnetic bead in test tube
Magnetic bar（2）.
2. tubular type magnetic bead separating device according to claim 1, it is characterised in that the magnetic bar（2）For cylindrical shape.
3. tubular type magnetic bead separating device according to claim 1, it is characterised in that the lower shoe（4）Provided with cylindrical shape
Neck（6）, the cylindric neck（6）Can hold test tube, and with the test tube hole（5）Test tube is fixed jointly.
4. tubular type magnetic bead separating device according to claim 3, it is characterised in that the cylindric neck（6）To be hollow
Cylindric neck.
5. tubular type magnetic bead separating device according to claim 3, it is characterised in that the cylindric neck（6）With test tube
Contact portion be the step structure that is gradually reduced from top to bottom of diameter, to hold different size of test tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720977512.2U CN206683922U (en) | 2017-08-07 | 2017-08-07 | A kind of tubular type magnetic bead separating device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720977512.2U CN206683922U (en) | 2017-08-07 | 2017-08-07 | A kind of tubular type magnetic bead separating device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206683922U true CN206683922U (en) | 2017-11-28 |
Family
ID=60407533
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720977512.2U Active CN206683922U (en) | 2017-08-07 | 2017-08-07 | A kind of tubular type magnetic bead separating device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206683922U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109336950A (en) * | 2018-11-23 | 2019-02-15 | 嘉兴领宇科技有限公司 | A kind of magnetic frame |
-
2017
- 2017-08-07 CN CN201720977512.2U patent/CN206683922U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109336950A (en) * | 2018-11-23 | 2019-02-15 | 嘉兴领宇科技有限公司 | A kind of magnetic frame |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6735276B2 (en) | System and method for collecting nucleic acid samples | |
| CN103370133B (en) | Use and filter the method isolating, gather, characterize and/or determining microorganism with sample transfer device | |
| JPWO2006132074A1 (en) | Liquid exchange method, component extraction method using the same, composite container and automatic analyzer | |
| JP6240785B2 (en) | System and method for sample concentration and detection | |
| JP2010529416A5 (en) | ||
| US9063044B2 (en) | Magnetic aggregating and washing device for in vitro assays and methods of use thereof | |
| CN201053965Y (en) | Influenza virus quick detection test paper | |
| CN201648403U (en) | Nucleic acid extraction apparatus without instruments | |
| CN206683922U (en) | A kind of tubular type magnetic bead separating device | |
| CN115161188A (en) | Air microorganism detection system and method | |
| CN103293049A (en) | Serum separation method and serum separation device | |
| CN201255708Y (en) | Magnetic separation device for high pass bioanalysis separation | |
| CN110023758A (en) | For separating equipment, system, method and the external member of analyte from body fluid sample | |
| CN108004233A (en) | A kind of negative pressure device available for automatic instrument for extracting nucleic acid | |
| CN206671035U (en) | A kind of cambered surface magnetic bead separating device | |
| CN201020402Y (en) | Detachable utility type magnetic separating rack | |
| CN106906121B (en) | The nucleic acid separator and separation method of magnet control | |
| CN202063922U (en) | Portable multi-functional magnetic separation device | |
| CN206684167U (en) | A kind of controllable magnetic bead separating device of magnetic | |
| CN2325115Y (en) | Apparatus for separating magnetic particles | |
| CN114736787A (en) | Nucleic acid elution and detection device | |
| CN206232701U (en) | Magnetic frame with liquid suction function | |
| CN207351784U (en) | A kind of 96 orifice plate magnetic bases | |
| CN108220233A (en) | Cell separation set surface treatment method, related utensil, peripheral blood rare cell or circulating tumor cell rapidly and efficiently separation method | |
| CN206375924U (en) | Nucleic acid purification magnetic frame |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |