CN220703714U - Gas-liquid exchange culture device for cell culture - Google Patents
Gas-liquid exchange culture device for cell culture Download PDFInfo
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- CN220703714U CN220703714U CN202321456538.4U CN202321456538U CN220703714U CN 220703714 U CN220703714 U CN 220703714U CN 202321456538 U CN202321456538 U CN 202321456538U CN 220703714 U CN220703714 U CN 220703714U
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- 238000004113 cell culture Methods 0.000 title claims abstract description 32
- 239000007788 liquid Substances 0.000 title claims abstract description 21
- 239000001963 growth medium Substances 0.000 claims abstract description 16
- 239000000463 material Substances 0.000 claims description 9
- 108010035532 Collagen Proteins 0.000 claims description 5
- 102000008186 Collagen Human genes 0.000 claims description 5
- 229920001436 collagen Polymers 0.000 claims description 5
- 108010082117 matrigel Proteins 0.000 claims description 5
- 239000011159 matrix material Substances 0.000 abstract description 9
- 238000000034 method Methods 0.000 abstract description 8
- 210000004748 cultured cell Anatomy 0.000 abstract description 4
- 239000002609 medium Substances 0.000 abstract description 4
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract description 2
- 210000001519 tissue Anatomy 0.000 description 8
- 238000013461 design Methods 0.000 description 6
- 238000000338 in vitro Methods 0.000 description 5
- 210000000056 organ Anatomy 0.000 description 5
- 210000000130 stem cell Anatomy 0.000 description 4
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 239000012612 commercial material Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Abstract
The utility model discloses a gas-liquid exchange culture device for cell culture, which comprises a culture plate body, wherein the culture plate body is provided with six large holes, small holes are sleeved on the inner sides of the six large holes, the large holes are connected with the bottoms of the small holes, and small slits are formed in the bottoms of the small holes. The beneficial effects of the utility model are as follows: the six large holes are formed in the culture plate body, so that the operation of other cultured cells in the cell culture process is convenient; the fixed connection of the large holes and the small holes is convenient for operation in the cell culture process, so that bubbles are prevented from occurring between the culture medium liquid and the matrix material due to improper addition mode of the culture medium, and the problems of medium pouring and the like caused in the large-volume culture medium culture process can be avoided; the small seam at the bottom of the small hole is designed, so that the medium and the matrix can be conveniently connected with each other, and the nutritional ingredients can be updated at any time.
Description
Technical Field
The utility model belongs to the technical field of stem cell in-vitro culture, and particularly relates to a gas-liquid exchange culture device for cell culture.
Background
In organisms, the growth of tissue and organs is in a 3D environment, and the growth of some tissues, especially the tissue and organs of epithelial systems, requires more nutrients and air to develop and mature. Therefore, the environment needs to be simulated in the stem cell in-vitro culture process, so that the stem cell in-vitro culture process is further used for promoting the in-vitro development of tissue organs, and a foundation is laid for the stem cell-based tissue engineering application;
at present, similar cell culture scaffolds for gas-liquid exchange already exist on the market, such as AVP005 of Alvetex family. The scaffold is mainly used for simulating extracellular matrix materials and is used for a 3D culture process of tissue organs such as skin and the like, which needs gas-liquid exchange. The bracket mainly utilizes plastic materials to sink matrix materials into holes of a cell culture plate, utilizes 3 extending brackets to be temporarily fixed at the top end of the cell culture plate, and maintains a certain space between the bottom of the bracket and the cell culture plate for adding a culture medium for cell culture. The bracket can effectively simulate a gas-liquid exchange system in the cell culture process, and realize the in-vitro 3D culture of tissue and organs;
however, the above solution still has certain drawbacks. Firstly, fixing a matrix material on the top end of a cell culture plate by using a bracket, which causes the problem of tightness of a cover of the culture plate and possibly has a certain pollution risk; secondly, the filling material in the bracket can only be commercial materials provided by companies, and the filling material cannot be rapidly prepared by self according to the self requirements; thirdly, in the process of simulating gas-liquid exchange, a proper amount of culture medium is required to be added at the bottom of the bracket to ensure gas-liquid exchange, but bubbles possibly appear at the bottom of the bracket in the process of adding the culture medium, so that the substrate materials in the bracket cannot be fully contacted with the culture medium, and the purpose of gas-liquid exchange cannot be achieved; finally, the expense of the prior art stents belongs to imported products, which severely restricts the application of the stents in the field of tissue engineering.
Disclosure of Invention
In view of the above-mentioned problems with the background art, the present utility model has as its object: aims to provide a gas-liquid exchange culture device for cell culture.
In order to achieve the technical purpose, the utility model adopts the following technical scheme:
the gas-liquid exchange culture device for cell culture comprises a culture plate body, wherein six large holes are formed in the culture plate body, small holes are sleeved on the inner sides of the large holes, the large holes are connected with the bottoms of the small holes, and small slits are formed in the bottoms of the small holes.
Further defined, six of said macropores are uniformly arranged on the culture plate body, wherein the spacing between two adjacent macropores is equal. Such a structural design facilitates manipulation with other cultured cells during cell culture.
Further defined, a culture space is formed between the large and small holes, and the culture space is used for adding a culture medium required for cell culture. Such a structural design facilitates the addition of the culture medium required for cell culture.
Further defined, the pores are filled with matrigel and collagen material. The structural design is convenient for filling matrigel and collagen materials for use.
The beneficial effects of the utility model are as follows:
1. the six large holes are formed in the culture plate body, so that the operation of other cultured cells in the cell culture process is convenient;
2. the fixed connection of the large holes and the small holes is convenient for operation in the cell culture process, so that bubbles are prevented from occurring between the culture medium liquid and the matrix material due to improper addition mode of the culture medium, and the problems of medium pouring and the like caused in the large-volume culture medium culture process can be avoided;
3. the small seam at the bottom of the small hole is designed, so that the medium and the matrix can be conveniently connected with each other, and the nutritional ingredients can be updated at any time.
Drawings
The utility model can be further illustrated by means of non-limiting examples given in the accompanying drawings;
FIG. 1 is a schematic diagram of a gas-liquid exchange culture apparatus for cell culture according to an embodiment of the present utility model;
FIG. 2 is a schematic cross-sectional view of a gas-liquid exchange culture apparatus for cell culture according to an embodiment of the present utility model;
the main reference numerals are as follows:
culture plate body 1, macropore 2, aperture 3, little seam 4, culture space 5.
Detailed Description
In order that those skilled in the art will better understand the present utility model, the following technical scheme of the present utility model will be further described with reference to the accompanying drawings and examples.
As shown in figures 1-2, the gas-liquid exchange culture device for cell culture is characterized in that a culture plate body 1 is provided with six large holes 2, small holes 3 are sleeved on the inner sides of the six large holes 2, the large holes 2 are connected with the bottoms of the small holes 3, and small slits 4 are formed in the bottoms of the small holes 3.
In this embodiment, the size of the culture plate body 1 is equal to that of a common 6-hole cell culture plate, so that the culture plate is convenient to operate together with other culture conditions, a small hole 3 is sleeved in each large hole 2, the large holes 2 are connected with the bottoms of the small holes 3, the addition of culture solution in the operation process is convenient, and the problems of tilting and the like of the culture caused by the inclination of the plate body are avoided, wherein a culture space 5 formed between the large holes 2 and the small holes 3 is used for adding cell culture medium, the small holes 3 can be filled with matrix materials according to the actual experimental requirements, and small slits 4 are formed at the bottoms of the small holes 3, so that the culture medium in the large holes 2 and the matrix in the small holes 3 are in nutritional communication.
Preferably, six large holes 2 are uniformly arranged on the culture plate body 1, wherein the interval between two adjacent large holes 2 is equal. Such a structural design facilitates manipulation with other cultured cells during cell culture.
Preferably, a culture space 5 is formed between the large holes 2 and the small holes 3, and the culture space 5 is used for adding a culture medium required for cell culture. Such a structural design facilitates the addition of the culture medium required for cell culture.
Preferably, the small holes 3 are used for filling matrigel and collagen materials. The structural design is convenient for filling matrigel and collagen materials for use.
The above embodiments are merely illustrative of the principles of the present utility model and its effectiveness, and are not intended to limit the utility model. Modifications and variations may be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the utility model. Accordingly, it is intended that all equivalent modifications and variations of the utility model be covered by the claims of this utility model, which are within the skill of those skilled in the art, can be made without departing from the spirit and scope of the utility model disclosed herein.
Claims (4)
1. A gas-liquid exchange culture device for cell culture, characterized in that: including cultivate plate body (1), cultivate plate body (1) is equipped with six macropores (2), six the inboard of macropore (2) all overlaps and is equipped with aperture (3), macropore (2) are connected with the bottom of aperture (3), the bottom of aperture (3) is equipped with small slit (4).
2. A gas-liquid exchange culture apparatus for cell culture according to claim 1, wherein: six macropores (2) are uniformly arranged on the culture plate body (1), wherein the distance between two adjacent macropores (2) is equal.
3. A gas-liquid exchange culture apparatus for cell culture according to claim 2, wherein: a culture space (5) is formed between the large hole (2) and the small hole (3), and the culture space (5) is used for adding a culture medium required by cell culture.
4. A gas-liquid exchange culture apparatus for cell culture according to claim 3, wherein: the small holes (3) are used for filling matrigel and collagen materials.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321456538.4U CN220703714U (en) | 2023-06-08 | 2023-06-08 | Gas-liquid exchange culture device for cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321456538.4U CN220703714U (en) | 2023-06-08 | 2023-06-08 | Gas-liquid exchange culture device for cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220703714U true CN220703714U (en) | 2024-04-02 |
Family
ID=90437122
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321456538.4U Active CN220703714U (en) | 2023-06-08 | 2023-06-08 | Gas-liquid exchange culture device for cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220703714U (en) |
-
2023
- 2023-06-08 CN CN202321456538.4U patent/CN220703714U/en active Active
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