CN201793576U - Novel cell culture device - Google Patents
Novel cell culture device Download PDFInfo
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- CN201793576U CN201793576U CN2010202987246U CN201020298724U CN201793576U CN 201793576 U CN201793576 U CN 201793576U CN 2010202987246 U CN2010202987246 U CN 2010202987246U CN 201020298724 U CN201020298724 U CN 201020298724U CN 201793576 U CN201793576 U CN 201793576U
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- cover plate
- cell culture
- silicagel pad
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- box
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Abstract
The utility model relates to a cell culture device comprising a culture box, a silica gel gasket, and a cover plate, wherein the cover plate is arranged on the silica gel gasket for sealing after the silica gel gasket is tightly covered on the culture box body, then screws are inserted in fixing locking screw holes arranged at four corners of the culture box, the silica gel gasket, and the cover plate for locking and tight sealing, and then the novel cell culture device is arranged in a three-dimensional gyrator for carrying out simulated experiment. The novel cell culture device is simple in structure and convenient to operate; and in the experimental research, the use amount of the cell culture medium is less, air bubbles are easy to discharge, and the sealing effect is good. All materials used by the cell culture device can tolerate hot and wet sterilization conditions, thus reducing possibility of microbial contamination. The used materials can be repeatedly used after washing, thus being beneficial to environmental protection. The novel cell culture device can be used for culturing common cells and also can be taken as a unique animal cell culture experiment device for research of simulating weightlessness biological effect.
Description
Technical field
The utility model relates to a kind of cell culture apparatus, specifically, relates to the experimental installation that simulated weightlessness biological effect in the life science is carried out animal cell culture.
Background technology
The environment of the space and the earth is diverse two kinds of environment, and weightlessness is one of principal feature of space environment.Under weightlessness, the different physiological roles disorders such as immunity system of unifying of spacefarer's skeletal musculature, cardiovascular system have a strong impact on spacefarer's safety, the human exploration to the outer space of influence.
Carry experiment by spaceflight and can study the effects of action of weightlessness biology.But this experiment condition chance seldom, and the condition harshness expends hugely, can not satisfy the requirement to steric effect further investigation.The scientific research personnel can carry out microgravity experiment research on ground, utilizes multiple emulation experiment method to come Simulated Weightlessness, and then the weightless influence to body of research.Common mode has two classes, and a class is based on falling bodies microgravity principle, as fall pipe, the tower that falls, aircraft parabolic flight, sounding rocket, balloon, free-falling machine etc.; Another kind of is effect in the ground simulation microgravity, hangs model, bed, two-dimentional gyrator and three-dimensional gyrator etc. as adopting mouse tail.
At present, when adopting three-dimensional gyrator simulated weightlessness biological effect, there is not special-purpose animal cell culture device, the cell culture apparatus that adopts other field to use usually.Culture apparatus commonly used has OptiCell, Tissue Culture Flask and inserted sheet box.
OptiCell is disposable using appts, costs an arm and a leg, and causes environmental pollution easily; When the revolution experiment, easily produce bubble, interference experiment effect.
When carrying out three-dimensional revolution experiment, must will cultivate chamber with substratum and fill, gas in the chamber is drained, and then guarantee that the cell aufwuchsplate has the liquid contact when bottle turns round always.If there is bubble will produce the shearing force of pair cell.Covered one deck liquid because Tissue Culture Flask is filled behind the substratum on the cell, be generally 1.8cm, experiment effect has been disturbed in the increase that the water purification that causes cell to bear is pressed; And the substratum large usage quantity, for example, 10cm
2Culture area need add the substratum of 22mL, when experiment needed to add some acting factor, it expended also more, has the problem of experiment reagent serious waste, and these acting factors are generally relatively more expensive.When culturing bottle is sealed, adopt silica gel plug jam-pack bottleneck simultaneously usually, tightly wrap then and seal film,, also introduce extra pressure, influence physiological status of cells though the sealing pattern can play the effect of sealing.
The inserted sheet box is not easy to operate when inserting the blood cover plate, and inserted sheet box material can not tolerate the moist heat sterilization condition, is easy to cause pollution; Simultaneously, the inserted sheet box also exists and is not easy to discharge bubble, the problem that the substratum consumption is big.
The utility model content
The technical problems to be solved in the utility model provides the experimental installation that a kind of three-dimensional gyrator simulated weightlessness biological effect is carried out animal cell culture.Characteristics of the present utility model are that apparatus structure is simple, use easy to operate; The substratum consumption is few in experimental study, is easy to discharge bubble.The utility model cell culture apparatus also can be used for conventional cell cultures.
Above-mentioned technical problem of the present utility model is by providing a kind of cell culture apparatus with following structure to solve, and this apparatus structure is made up of three parts, comprises cultivating box, silicagel pad and cover plate.
Be provided with three measure-alike cells in the described cultivation box, not connected between three cells.The broadside of each cell respectively has the dark little notch of 1mm, can embed the blood cover plate of 22 * a 26 * 0.45mm by two notches, and growth has the one side of cell in the middle of cell.Cultivate three cells that are provided with in the box and can place a blood cover plate separately, promptly once experiment can obtain three repeat samples and compares.
For reducing the substratum consumption, reduce the liquid water purification and press, must reduce the thickness of nutrient solution, the substratum of 4.0mm thickness promptly can satisfy 5 * 10
4The cells/mL cell is cultivated the 72h culture volume under closed environment.But, can cause substratum souring rapidly in cell growth process if further reduce thickness, influence the cell growth.
The notch top of inserting the blood cover plate is oblique cutting degree of lip-rounding shape, and top is wide to be 1.71mm, and oblique becoming during down to 7mm is 0.6mm, and promptly wide bottom, top is narrow, and this structural pattern is very easy.Last face width is convenient to the blood cover plate is placed into the place that will insert, and light one presses, and the blood cover plate just can be inserted into the bottom; And following narrow, under the capillary effect of water, can play fixed action.
The blood cover plate is meeting and the tight adhesion of cultivation box wall in the aqueous solution, takes out the blood cover plate for convenience, and there are the little slotted eye that communicates, groove depth 1.5mm in the long limit of each cell bottom and insertion blood cover plate.At first can use tweezers that the blood cover plate is taken out; If blood cover plate and little locular wall stick tension, can adopt special steel wire hook to go out from the bottom with blood cover plate hook.
Introduce external pressure when testing for fear of turning round, the mode that adds the sheet-steel cover sealing after the employing silicagel pad compresses is sealed.Described silicagel pad is by the medical silica-gel press forming, can play good sealing effectiveness during use, can also tolerate the moist heat sterilization condition, generally is not less than 121 ℃, 20min.
In order to increase sealing effectiveness, be provided with the little edge of projection at the edge of cultivating each cell opening of box, the wide of little edge is 0.45mm, the high 0.5mm of being.After depressing silicagel pad, little edge can embed silicagel pad, the sealing of strengthening silicagel pad and cultivating box cell mouth.
Described cultivation box opening is square, and the degree of depth is 3mm.To cultivate the box opening portion be trapezoidal and silicagel pad is pressed into, thickness is 3.5mm, the width on top is identical with the width of cultivating the box opening, the thickness of silicagel pad is than the big 0.5mm of the degree of depth that cultivates the box opening, can be so that silicagel pad and cultivation box cell peristome in conjunction with closely, can guarantee that fully the top of cultivating the box opening seals.Trapezoid design can so that silicagel pad when pressing down to around extruding, seal more tight.
The material of cultivating box is a polycarbonate, has better biocompatibility, can tolerate the moist heat sterilization condition.Can reuse, reduce environmental pollution.
Described cover plate is to manufacture shaping by stainless material, is used for enhanced leaktightness, can tolerate the moist heat sterilization condition, generally is not less than 121 ℃, 20min.Easy to use.
Adopt the cell culture apparatus of said structure, not only it is simple in structure, and easy to operate.It can reduce cell culture medium usage quantity, be easy to discharge bubble and good sealing effect.The employed material of cell culture apparatus all can tolerate the moist heat sterilization condition, has reduced the possibility of microbial contamination, can also reuse simultaneously.
The utility model cell culture apparatus can be used for conventional cell cultures, especially is suitable for three-dimensional gyrator simulated weightlessness biological effect and carries out animal cell culture, for the research of simulated weightlessness biological effect provides a kind of unique animal cell culture experimental installation.
Description of drawings
Below in conjunction with accompanying drawing the utility model cell culture apparatus is described in further detail.
Fig. 1 is the cultivation box structural representation of the utility model cell culture apparatus.
Fig. 2 is the cultivation box sectional view of the utility model cell culture apparatus.
Fig. 3 is the silicagel pad synoptic diagram of the utility model cell culture apparatus.
Fig. 4 is the silicagel pad left side view of the utility model cell culture apparatus.
Fig. 5 is the cover plate synoptic diagram of the utility model cell culture apparatus.
Fig. 6 is the cover plate left side view of the utility model cell culture apparatus.
1. cultivate box 2. cells 3. notches 4. notches
5. notch 6. little edge 7. screw holes 8. are cultivated the box opening
Embodiment
Present embodiment is a kind of device that the simulated weightlessness biological effect is carried out cell cultures that is used for, and apparatus structure comprises cultivates box, silicagel pad and cover plate.
As seen from Figure 1, Figure 2, be provided with measure-alike, three cells 2 independently separately in the cultivation box 1 of the utility model cell culture apparatus, each cell 2 is not connected, can fill substratum.The long 20.40mm of cell, wide 4.50mm, dark 26mm.The broadside of each cell respectively has a dark little notch of 1mm, can place the blood cover plate of 22 * a 26 * 0.45mm by the both sides notch 3 of cell 2, and growth has the one side of cell in the middle of cell.There is a notch 4 at the back of blood cover plate, and a notch 5 is also arranged at the bottom of blood cover plate.Be provided with the little edge 6 of a circle projection at the edge of cultivating each cell opening of box, little edge wide be 0.45mm, highly be 0.5mm, depress silicagel pad after, little edge 6 can embed silicagel pad, the sealing of reinforcement silicagel pad and cell opening.Be provided with fixing and locking screw hole 7 at four jiaos that cultivate box body.
Fig. 3 and Fig. 4 have showed the silicagel pad of the utility model cell culture apparatus.Described silicagel pad embeds and cultivates the box opening portion is trapezium-shaped, and thickness is 3.5mm.The cultivation box opening 8 of cell culture apparatus is a square configuration, and the degree of depth is 3mm.The width on silicagel pad top is identical with the width of cultivating box opening 8, and the thickness of silicagel pad is than the big 0.5mm of the degree of depth that cultivates box opening 8.When the design of trapezium-shaped can make silicagel pad press down to around extruding, the top seal that can guarantee to cultivate box opening 8 fully is more tight.
Described silicagel pad is made by medical silica-gel, can play good sealing function during use, can also tolerate the moist heat sterilization condition, generally is not less than 121 ℃, 20min.
The utility model cell culture apparatus, after cultivation box, silicagel pad and cover plate adopt the moist heat sterilization sterilization respectively, have the blood cover plate of zooblast to insert the both sides funnel-form notch 3 of cultivating box cell 2 growth in advance, growth has the one side of cell in the middle of cell 2.Inserting the top of both sides notch 3 of blood cover plate wide be 1.71mm, narrows down gradually for oblique time, becomes during to the 7mm place to be 0.6mm, promptly forms a narrow oblique cutting mouth shape structure of wide bottom, top, and its structural pattern is very easy.Top is wide, is convenient to insert the blood cover plate; The bottom is narrow, only than the wide 0.15mm of the thickness of blood cover plate, under the capillary effect of water, can play the effect of fix blood cover plate.
The blood cover plate is meeting and the tight adhesion of cultivation box wall in the aqueous solution, takes out the blood cover plate for convenience, and there are the little slotted eye that communicates, groove depth 1.5mm in the long limit of each cell bottom and insertion blood cover plate.At first can use tweezers the blood cover plate to be taken out from blood cover plate back notch 4; If blood cover plate and little locular wall stick tension, can adopt special steel wire hook blood cover plate hook to be gone out from bottom plate rabbet 5.
According to requirement of experiment, three the measure-alike cells that are provided with in the cultivation box of the utility model cell culture apparatus 1 can be placed a blood cover plate separately, and promptly once experiment can obtain three repeat samples and compares; Can also select to insert 1～3 blood cover plate experiment that does not wait.
The preheating substratum is added the cell that is inserted with the blood cover plate, and the substratum liquid level will be higher than the little edge 6 of cell edge of opening projection, uses transfer pipet to inhale and abandons open bubble.
Referring to Fig. 5, Fig. 6, the cover plate of the utility model cell culture apparatus, its shape with cultivate the suitable for reading and silicagel pad of box body and match identically, Qi Sijiao is provided with the screw hole 7 of fixing and locking, so that insert screw locking.After using the silicagel pad lid tight on the cultivation box body, add a cover the mode of cover plate for sealing and seal, the 7 insertion screw lockings of four jiaos of fixed screw holes that are provided with of box body are obturaged.After the encapsulation, can place three-dimensional gyrator to carry out the experiment of simulated weightlessness biological effect.
Described cover plate is to manufacture shaping by stainless material, is used for enhanced leaktightness, can tolerate the moist heat sterilization condition, generally is not less than 121 ℃, 20min.
After experiment disposes, open cover plate and silica gel pad, use tweezers the blood cover plate to be taken out, if blood cover plate and little locular wall stick tension from blood cover plate back notch 4, also can adopt steel wire hook blood cover plate hook to be gone out, carry out follow-up experiment and detect from blood cover plate bottom plate rabbet 5.
Cultivation box, silicagel pad and the cover plate of the utility model cell culture apparatus, reusable after cleaning, help environment protection.
Claims (4)
1. a cell culture apparatus is characterized in that comprising cultivation box, silicagel pad and cover plate; Cultivate in the box (1) and be provided with measure-alike three cells (2), the sulculus that communicates is arranged at the one side and the bottom of each cell (2), places a blood cover plate separately by cell both sides notches (3); The notch (3) of insertion blood cover plate is an oblique cutting degree of lip-rounding shape, and top is wide, the narrow convenient blood cover plate that inserts in bottom, can play fixed action simultaneously, and the bottom of cell (2) and placement blood cover plate one side respectively have a notch, the convenient blood cover plate that takes out; The edge of opening of cultivating box cell (2) is provided with the little edge (6) of a circle projection, depress silicagel pad after, little edge (6) can embed silicagel pad, makes the sealing of silicagel pad and cell mouth, and can get rid of open bubble; After using the silicagel pad lid tight on the cultivation box body, on silicagel pad, use cover plate for sealing, will cultivate box (1), silicagel pad and four jiaos of fixing and locking screw holes (7) insertion screw lockings that are provided with of cover plate and obturage, place three-dimensional gyrator to carry out simulated experiment.
2. cell culture apparatus according to claim 1, it is characterized in that: described cultivation box opening (8) is for square, silicagel pad is inserted and is cultivated box opening (8) part for trapezoidal, the width on silicagel pad top is identical with the width of cultivating box opening (8), silicagel pad is pressed down and to around crush seal.
3. cell culture apparatus according to claim 1 is characterized in that: described cultivation box (1) material is a polycarbonate; Described silicagel pad adopts the medical silica-gel press forming; Described cover plate is made by stainless material and is shaped; It all can tolerate the moist heat sterilization condition is 121 ℃, 20min.
4. cell culture apparatus according to claim 1 is characterized in that: the culture volume of cultivating box cell (2) is less than 3mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010202987246U CN201793576U (en) | 2010-08-19 | 2010-08-19 | Novel cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010202987246U CN201793576U (en) | 2010-08-19 | 2010-08-19 | Novel cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201793576U true CN201793576U (en) | 2011-04-13 |
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ID=43848495
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010202987246U Expired - Lifetime CN201793576U (en) | 2010-08-19 | 2010-08-19 | Novel cell culture device |
Country Status (1)
| Country | Link |
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| CN (1) | CN201793576U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102373154A (en) * | 2010-08-19 | 2012-03-14 | 西北工业大学 | Novel cell culturing apparatus |
| CN106047693A (en) * | 2016-06-25 | 2016-10-26 | 李万 | Multifunctional intelligent cell culture dish |
| CN109490056A (en) * | 2018-10-31 | 2019-03-19 | 程辉 | A kind of cured embedding method of three-dimensional cell cultivation matrigel |
| CN113388517A (en) * | 2021-06-08 | 2021-09-14 | 北京理工大学 | Biological culture micro-fluidic chip suitable for assembling microgravity gyroscope and cell culture method thereof |
-
2010
- 2010-08-19 CN CN2010202987246U patent/CN201793576U/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102373154A (en) * | 2010-08-19 | 2012-03-14 | 西北工业大学 | Novel cell culturing apparatus |
| CN106047693A (en) * | 2016-06-25 | 2016-10-26 | 李万 | Multifunctional intelligent cell culture dish |
| CN106047693B (en) * | 2016-06-25 | 2018-02-13 | 广东希瑞干细胞技术有限公司 | Multifunctional intellectual Tissue Culture Dish |
| CN109490056A (en) * | 2018-10-31 | 2019-03-19 | 程辉 | A kind of cured embedding method of three-dimensional cell cultivation matrigel |
| CN109490056B (en) * | 2018-10-31 | 2021-07-30 | 程辉 | Cell three-dimensional culture matrigel wax embedding method |
| CN113388517A (en) * | 2021-06-08 | 2021-09-14 | 北京理工大学 | Biological culture micro-fluidic chip suitable for assembling microgravity gyroscope and cell culture method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20110413 Effective date of abandoning: 20130424 |
|
| RGAV | Abandon patent right to avoid regrant |