CN102373154B - Novel cell culturing apparatus - Google Patents
Novel cell culturing apparatus Download PDFInfo
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- CN102373154B CN102373154B CN 201010258334 CN201010258334A CN102373154B CN 102373154 B CN102373154 B CN 102373154B CN 201010258334 CN201010258334 CN 201010258334 CN 201010258334 A CN201010258334 A CN 201010258334A CN 102373154 B CN102373154 B CN 102373154B
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- cover plate
- cell
- box
- silicagel pad
- cell culture
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Abstract
The invention discloses a novel cell culturing apparatus comprising a culturing box, a silica gel pad and a cover plate. According to the invention, the culturing box is firmly covered by using the silica gel pad, and the culturing box is sealed by using the cover plate on the silica gel pad. Screws are inserted in fixing and locking screw holes arranged on four corners of the culturing box, the silica gel pad and the cover plate, such that the cell culturing apparatus is tightly locked. The cell culturing apparatus is subject to a simulation experiment in a three-dimensional clinostat. The apparatus provided by the invention is advantaged in simple structure and convenient operation. During an empirical study process, the application amount of cell culture media is low, bubbles are easy to discharge, and the sealing effect is good. The materials adopted by the cell culturing apparatus are tolerant to a moist heat sterilization condition, such that the possibility of microbial contamination is reduced. The used materials can be reused after cleaning, such that the apparatus is beneficial for environment protection. The cell culturing apparatus provided by the invention can be applied in common cell culturing, and also provides a unique animal cell cultivating apparatus for simulated weightless biological effect researches.
Description
Technical field
The present invention relates to a kind of cell culture apparatus, specifically, relate to the experimental installation that simulated weightlessness biological effect in the life science is carried out animal cell culture.
Background technology
The environment of space and the earth is diverse two kinds of environment, and weightlessness is one of principal feature of space environment.Under weightlessness, spacefarer's skeletal musculature, the cardiovascular system different physiological roles such as immunity system of unifying is disorderly, has a strong impact on spacefarer's safety, the human exploration to the outer space of impact.
Carry experiment by spaceflight and can study weightlessness to the effects of action of biology.But this experiment condition chance seldom, and condition is harsh, expends hugely, can not satisfy the requirement to the steric effect further investigation.The scientific research personnel can carry out on ground microgravity experiment research, utilizes multiple emulation experiment method to come Simulated Weightlessness, and then the weightless impact on body of research.Common mode has two classes, and a class is based on falling bodies microgravity principle, as fall pipe, the tower that falls, aircraft parabolic flight, sounding rocket, balloon, free-falling machine etc.; Another kind of is effect in the ground simulation microgravity, hangs model, bed, two-dimentional gyrator and three-dimensional gyrator etc. as adopting mouse tail.
At present, when adopting three-dimensional gyrator simulated weightlessness biological effect, there is not special-purpose animal cell culture device, the cell culture apparatus that usually adopts other field to use.Culture apparatus commonly used has OptiCell, Tissue Culture Flask and insert box.
OptiCell is disposable using appts, and is expensive, easily causes environmental pollution; When the revolution experiment, easily produce bubble, interference experiment effect.
When carrying out three-dimensional revolution experiment, must will cultivate chamber with substratum and fill, gas in the chamber is drained, and then guarantee that the Growth of Cells face has the liquid contact when bottle turns round always.If there is bubble will produce shearing force to cell.Covered one deck liquid because Tissue Culture Flask is filled behind the substratum on the cell, be generally 1.8cm, experiment effect has been disturbed in the increase that the water purification that causes cell to bear is pressed; And the substratum large usage quantity, for example, 10cm
2Culture area need to add the substratum of 22mL, when experiment needed to add some acting factor, it expended also more, has the problem of experiment reagent serious waste, and these acting factors are generally relatively more expensive.When culturing bottle is sealed, usually adopt simultaneously silica gel plug jam-pack bottleneck, then tightly wrap sealed membrane, although the sealing pattern can play the effect of sealing, also introduce extra pressure, affect the physiological status of cell.
Insert box is not easy to operate when inserting the blood cover plate, and the insert box material can not tolerate the moist heat sterilization condition, is easy to cause pollution; Simultaneously, insert box also exists and is not easy to discharge bubble, the problem that the substratum consumption is large.Summary of the invention
The technical problem to be solved in the present invention provides the experimental installation that a kind of three-dimensional gyrator simulated weightlessness biological effect is carried out animal cell culture.Characteristics of the present utility model are that apparatus structure is simple, use easy to operate; The substratum consumption is few in experimental study, is easy to discharge bubble.The utility model cell culture apparatus also can be used for conventional cell cultures.
Above-mentioned technical problem of the present invention is by providing a kind of cell culture apparatus with following structure to solve, and this apparatus structure is comprised of three parts, comprises cultivating box, silicagel pad and cover plate.
Be provided with three measure-alike cells in the described cultivation box, not connected between three cells.The broadside of each cell respectively has the dark small-rabbet of 1mm, can embed the blood cover plate of 22 * a 26 * 0.45mm by two notches, and growth has the one side of cell in the middle of cell.Cultivate three cells that arrange in the box and can place separately a blood cover plate, namely once experiment can obtain three repeat samples and compares.
For reducing the substratum consumption, reduce the liquid water purification and press, must reduce the thickness of nutrient solution, the substratum of 4.0mm thickness namely can satisfy 5 * 10
4The cells/mL cell is cultivated the 72h culture volume under closed environment.But, can cause substratum rapidly souring in cell growth process if further reduce thickness, affect Growth of Cells.
The notch top of inserting the blood cover plate is the oblique cutting mouth-shaped, and top is wide to be 1.71mm, and oblique becoming during down to 7mm is 0.6mm, and namely wide bottom, top is narrow, and this structural pattern is very easy.Upper face width is convenient to the blood cover plate is placed into the place that will insert, and light one presses, and the blood cover plate just can be inserted into the bottom; And following narrow, under the capillary effect of water, can play fixed action.
The blood cover plate is meeting and the tight adhesion of cultivation box wall in the aqueous solution, and for the convenient blood cover plate that takes out, there are the little slotted eye that communicates, groove depth 1.5mm in the long limit of each cell bottom and insertion blood cover plate.At first can use tweezers that the blood cover plate is taken out; If blood cover plate and little locular wall stick tension, can adopt special steel wire hook to go out from the bottom with blood cover plate hook.
Introduce external pressure when testing for fear of turning round, the mode that adds the sheet-steel cover sealing after the employing silicagel pad compresses is sealed.Described silicagel pad is by the medical silica-gel press forming, can play good sealing effectiveness during use, can also tolerate the moist heat sterilization condition, generally is not less than 121 ℃, 20min.
In order to increase sealing effectiveness, be provided with the little edge of projection at the edge of cultivating each cell opening of box, the wide of little edge is 0.45mm, the high 0.5mm of being.After depressing silicagel pad, little edge can embed silicagel pad, the sealing of strengthening silicagel pad and cultivating box cell mouth.
Described cultivation box opening is square, and the degree of depth is 3mm.To cultivate the box opening portion be trapezoidal and silicagel pad is pressed into, thickness is 3.5mm, the width on top is identical with the width of cultivating the box opening, the Thickness Ratio of silicagel pad is cultivated the large 0.5mm of the degree of depth of box opening, can be so that silicagel pad and cultivation box cell peristome in conjunction with closely, can guarantee that fully the top of cultivating the box opening seals.Trapezoidal design can so that silicagel pad when pressing down to around extruding, seal more tight.
The material of cultivating box is polycarbonate, has preferably biocompatibility, can tolerate the moist heat sterilization condition.Can reuse, reduce environmental pollution.
Described cover plate is to manufacture shaping by stainless material, is used for enhanced leaktightness, can tolerate the moist heat sterilization condition, generally is not less than 121 ℃, 20min.Easy to use.
Adopt the cell culture apparatus of said structure, not only it is simple in structure, and easy to operate.It can reduce cell culture medium usage quantity, be easy to discharge bubble and good sealing effect.The employed material of cell culture apparatus all can tolerate the moist heat sterilization condition, has reduced the possibility of microbial contamination, can also reuse simultaneously.
Cell culture apparatus of the present invention can be used for conventional cell cultures, and especially applicable three-dimensional gyrator simulated weightlessness biological effect is carried out animal cell culture, for the research of simulated weightlessness biological effect provides a kind of unique animal cell culture experimental installation.
Description of drawings
Below in conjunction with accompanying drawing cell culture apparatus of the present invention is described in further detail.
Fig. 1 is the cultivation box structural representation of cell culture apparatus of the present invention.
Fig. 2 is the cultivation box sectional view of cell culture apparatus of the present invention.
Fig. 3 is the silicagel pad schematic diagram of cell culture apparatus of the present invention.
Fig. 4 is the silicagel pad left side view of cell culture apparatus of the present invention.
Fig. 5 is the cover plate schematic diagram of cell culture apparatus of the present invention.
Fig. 6 is the cover plate left side view of cell culture apparatus of the present invention.
1. cultivate box 2. cells 3. notches 4. notches
5. notch 6. little edge 7. screw holes 8. are cultivated the box opening
Embodiment
The present embodiment is a kind of device that carries out cell cultures for the simulated weightlessness biological effect, and apparatus structure comprises cultivates box, silicagel pad and cover plate.
As seen from Figure 1, Figure 2, be provided with measure-alike, three cells 2 independently separately in the cultivation box 1 of cell culture apparatus of the present invention, each cell 2 is not connected, can fill substratum.The long 20.40mm of cell, wide 4.50mm, dark 26mm.The broadside of each cell respectively has a dark small-rabbet of 1mm, can place the blood cover plate of 22 * a 26 * 0.45mm by the both sides notch 3 of cell 2, and growth has the one side of cell in the middle of cell.There is a notch 4 at the back of blood cover plate, and a notch 5 is also arranged at the bottom of blood cover plate.Be provided with the little edge 6 of a circle projection at the edge of cultivating each cell opening of box, little edge wide be 0.45mm, highly be 0.5mm, depress silicagel pad after, little edge 6 can embed silicagel pad, the sealing of reinforcement silicagel pad and cell opening.Be provided with fixing and locking screw hole 7 at four jiaos that cultivate box body.
Fig. 3 and Fig. 4 have showed the silicagel pad of cell culture apparatus of the present invention.Described silicagel pad embeds and cultivates the box opening portion is trapezium-shaped, and thickness is 3.5mm.The cultivation box opening 8 of cell culture apparatus is square configuration, and the degree of depth is 3mm.The width on silicagel pad top is identical with the width of cultivating box opening 8, and the Thickness Ratio of silicagel pad is cultivated the large 0.5mm of the degree of depth of box opening 8.When the design of trapezium-shaped can make silicagel pad press down to around extruding, the top seal that can guarantee to cultivate box opening 8 fully is more tight.
Described silicagel pad is made by medical silica-gel, can play good sealing function during use, can also tolerate the moist heat sterilization condition, generally is not less than 121 ℃, 20min.
Cell culture apparatus of the present invention after cultivation box, silicagel pad and cover plate adopt respectively the moist heat sterilization sterilization, has the blood cover plate of zooblast to insert the both sides funnel-form notch 3 of cultivating box cell 2 in advance growth, and growing has the one side of cell in the middle of cell 2.Inserting the top of both sides notch 3 of blood cover plate wide be 1.71mm, narrows down gradually for oblique time, becomes during to the 7mm place to be 0.6mm, namely forms a narrow oblique cutting mouth shape structure of wide bottom, top, and its structural pattern is very easy.Top is wide, is convenient to insert the blood cover plate; The bottom is narrow, only than the wide 0.15mm of the thickness of blood cover plate, under the capillary effect of water, can play the effect of fix blood cover plate.
The blood cover plate is meeting and the tight adhesion of cultivation box wall in the aqueous solution, and for the convenient blood cover plate that takes out, there are the little slotted eye that communicates, groove depth 1.5mm in the long limit of each cell bottom and insertion blood cover plate.At first can use tweezers from blood cover plate back notch 4 the blood cover plate to be taken out; If blood cover plate and little locular wall stick tension, can adopt special steel wire hook from bottom plate rabbet 5 blood cover plate hook to be gone out.
According to requirement of experiment, three measure-alike cells of the cultivation box 1 interior setting of the utility model cell culture apparatus can be placed a blood cover plate separately, and namely once experiment can obtain three repeat samples and compares; Can also select to insert 1～3 blood cover plate experiment that does not wait.
The preheating substratum is added the cell that is inserted with the blood cover plate, and the substratum liquid level will be higher than the little edge 6 of cell edge of opening projection, uses transfer pipet to inhale and abandons open bubble.
Referring to Fig. 5, Fig. 6, the cover plate of cell culture apparatus of the present invention, its shape with cultivate the suitable for reading and silicagel pad of box body and match identically, Qi Sijiao is provided with the screw hole 7 of fixing and locking, in order to insert screw locking.After the cultivation box body uses silicagel pad to cover tightly, add a cover the mode of cover plate for sealing and seal, four jiaos of fixed screw holes that arrange of box body 7 are inserted screw lockings obturage.After the encapsulation, can place three-dimensional gyrator to carry out the experiment of simulated weightlessness biological effect.
Described cover plate is to manufacture shaping by stainless material, is used for enhanced leaktightness, can tolerate the moist heat sterilization condition, generally is not less than 121 ℃, 20min.
After experiment is disposed, open cover plate and silica gel pad, use tweezers from blood cover plate back notch 4 the blood cover plate to be taken out, if blood cover plate and little locular wall stick tension, also can adopt steel wire hook from blood cover plate bottom plate rabbet 5 blood cover plate hook to be gone out, carry out follow-up experiment and detect.
Cultivation box, silicagel pad and the cover plate of cell culture apparatus of the present invention, reusable after cleaning, be conducive to environment protection.
Claims (4)
1. a cell culture apparatus is characterized in that comprising cultivation box, silicagel pad and cover plate; Cultivate in the box (1) and be provided with measure-alike three cells (2), the sulculus that communicates is arranged at one side and the bottom of each cell (2), places separately a blood cover plate by cell both sides notches (3); The notch (3) of insertion blood cover plate is the oblique cutting mouth-shaped, and top is wide, the narrow convenient blood cover plate that inserts in bottom, can play fixed action simultaneously, and the bottom of cell (2) and placement blood cover plate one side respectively have a notch, the convenient blood cover plate that takes out; The edge of opening of cultivating box cell (2) is provided with the little edge (6) of a circle projection, depress silicagel pad after, little edge (6) can embed silicagel pad, makes the sealing of silicagel pad and cell mouth, and can get rid of open bubble; After using silicagel pad to cover tightly on the cultivation box body, on silicagel pad, use cover plate for sealing, will cultivate box (1), silicagel pad and four jiaos of fixing and locking screw holes (7) insertion screw lockings that arrange of cover plate and obturage, place three-dimensional gyrator to carry out simulated experiment.
2. cell culture apparatus according to claim 1, it is characterized in that: cultivate box opening (8) for square, silicagel pad is inserted and is cultivated box opening (8) part for trapezoidal, the width on silicagel pad top is identical with the width of cultivating box opening (8), silicagel pad is pressed down and to around crush seal.
3. cell culture apparatus according to claim 1, it is characterized in that: described cultivation box (1) material is polycarbonate; Described silicagel pad adopts the medical silica-gel press forming; Described cover plate is made by stainless material and is shaped; It all can tolerate the moist heat sterilization condition is 121 ℃, 20min.
4. cell culture apparatus according to claim 1 is characterized in that: cultivate the culture volume of box cell (2) less than 3mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010258334 CN102373154B (en) | 2010-08-19 | 2010-08-19 | Novel cell culturing apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010258334 CN102373154B (en) | 2010-08-19 | 2010-08-19 | Novel cell culturing apparatus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102373154A CN102373154A (en) | 2012-03-14 |
| CN102373154B true CN102373154B (en) | 2013-04-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010258334 Expired - Fee Related CN102373154B (en) | 2010-08-19 | 2010-08-19 | Novel cell culturing apparatus |
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| Country | Link |
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| CN (1) | CN102373154B (en) |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2002367057B2 (en) * | 2001-12-21 | 2007-01-11 | Organogenesis Inc. | Chamber with adjustable volume for cell culture and organ assist |
| US20040214313A1 (en) * | 2003-04-28 | 2004-10-28 | Weihua Zhang | Cell interaction culture system and uses thereof |
| CN200952014Y (en) * | 2006-09-06 | 2007-09-26 | 中国航天员科研训练中心 | Inserting sheet type cultivate bottle |
| CN101221166B (en) * | 2008-01-22 | 2011-09-14 | 西北工业大学 | Cell strain loading device under three-dimensional cultivation condition |
| CN201195726Y (en) * | 2008-02-19 | 2009-02-18 | 中国航天员科研训练中心 | Combined cell culture bottle |
| CN201793576U (en) * | 2010-08-19 | 2011-04-13 | 西北工业大学 | Novel cell culture device |
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2010
- 2010-08-19 CN CN 201010258334 patent/CN102373154B/en not_active Expired - Fee Related
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| CN102373154A (en) | 2012-03-14 |
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| C14 | Grant of patent or utility model | ||
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| TR01 | Transfer of patent right |
Effective date of registration: 20170918 Address after: 510000, A, 5 Changxin Road, Tianhe District, Guangdong, Guangzhou Patentee after: Guangzhou Boren Health Medical Technology Co Ltd Address before: 710072 Xi'an friendship West Road, No. 646, Shaanxi, box 127 Patentee before: Northwestern Polytechnical University |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130424 Termination date: 20190819 |