CN220405677U - Flow cytometry reagent extraction device - Google Patents
Flow cytometry reagent extraction device Download PDFInfo
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- CN220405677U CN220405677U CN202321550982.2U CN202321550982U CN220405677U CN 220405677 U CN220405677 U CN 220405677U CN 202321550982 U CN202321550982 U CN 202321550982U CN 220405677 U CN220405677 U CN 220405677U
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- 238000000605 extraction Methods 0.000 title claims abstract description 53
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 38
- 238000000684 flow cytometry Methods 0.000 title claims abstract description 19
- 238000012360 testing method Methods 0.000 claims abstract description 62
- 238000007789 sealing Methods 0.000 claims abstract description 35
- 239000000725 suspension Substances 0.000 claims abstract description 12
- 238000009413 insulation Methods 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 4
- 238000001514 detection method Methods 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 241000973497 Siphonognathus argyrophanes Species 0.000 abstract 1
- 230000002265 prevention Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 3
- 230000002572 peristaltic effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000002798 bone marrow cell Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to the technical field of medical detection, and provides a reagent extraction device for flow cytometry, which comprises: the test tube periphery is equipped with the cardboard, the test tube top is equipped with suspension extraction device, the inside heat insulating board that is equipped with of test tube, the test tube bottom is equipped with the mounting, and the sealing device is equipped with to the mounting below, through suspension extraction device's setting, suspension extraction device comprises extraction board, sealing ring, drawing tube, gas vent and main part etc. the extraction board is used for sealing the inside prevention circulation of air of test tube, after getting the reagent, sealing ring is used for sealing the test tube mouth and prevents the pollution, through the button extraction air in the main part, air in the drawing tube is discharged from the gas vent, in order to reach the effect of fixing the drawing tube on the extraction board and realizing the pulling, the scale value that the drawing tube is marked on can carry out the extraction of reagent according to the demand.
Description
Technical Field
The utility model relates to the technical field of medical detection, in particular to a reagent extraction device for flow cytometry.
Background
In practical application, the flow cytometry works on the principle of multi-parameter and rapid quantitative analysis of single cells or other biological particles by monoclonal antibodies at the cellular molecular level. The method can analyze tens of thousands of cells at high speed, can simultaneously measure a plurality of parameters from one cell, has the advantages of high speed, high precision and good accuracy, and is one of the most advanced cell quantitative analysis technologies in the current generation. Light sources, flow channels, signal detection transmission and data analysis systems are the main components of flow cytometry. At present, the detection of peripheral blood leucocytes, bone marrow cells, tumor cells and the like by using a flow cytometer is an important component of clinical detection.
The prior art can refer to the publication number: CN206832825U, which is a chinese patent for utility model, discloses a reagent extracting device for trace element detector, comprising a supporting base, a butt-joint disc, a contact switch, an infrared positioning device, a PLC controller and a stepping motor, wherein the butt-joint disc is arranged on the supporting base, the butt-joint disc is connected with a rotating disc through a rotating shaft, the rotating disc is controlled by the stepping motor through a synchronous belt transmission, the upper part of the rotating disc is in threaded connection with a support body, an electromagnetic driving valve is arranged on the support body, the electromagnetic driving valve is connected with a piston valve through a transmission mechanism, the bottom liquid inlet end of the piston valve is connected with a communicating vessel through a rigid cannula switch, the liquid outlet of the piston valve is connected with an external peristaltic pump, and the communicating vessel is connected with an external reagent barrel through a pipeline; the peristaltic pump has the advantages of simple structure, high automation degree and convenient use, the piston valve can be accurately and quickly connected and switched with different reagent barrels, different reagents can be accurately and quickly extracted by the same peristaltic pump, the experimental requirements are met, and the working efficiency is improved.
The current flow cytometry is with reagent extraction needs to draw multiple different reagents, needs the ration when different reagent draws, and current extraction device only can rely on the estimation, and is not accurate enough, and need constantly change extraction device when extracting multiple reagent, prevents to mix, has influenced reagent extraction efficiency, has increased the wasting of resources.
Disclosure of Invention
The utility model provides a reagent extraction device for flow cytometry, which solves the problems of reagent extraction, quantification and storage in the related technology.
The technical scheme of the utility model is as follows:
a flow cytometry reagent extraction device comprising: the test tube is characterized in that a clamping plate is arranged on the periphery of the test tube, a suspension extracting device is arranged above the test tube, a heat insulation plate is arranged inside the test tube, a fixing piece is arranged at the bottom of the test tube, and a sealing device is arranged below the fixing piece;
the suspension extraction device comprises: the drawing tube is arranged at the upper end of the drawing tube, the drawing tube is connected with the groove, a button is arranged at the left side below the main body, and an exhaust port is arranged at the right side of the main body;
the sealing device comprises: the bottom plate is arranged below the fixing piece, the clamping groove is formed in the periphery of the bottom plate, the leakage-proof piece is arranged on the outer layer of the clamping groove, and the locking piece is arranged at the upper end of the leakage-proof piece.
Preferably, the outer structure of the test tube is an adduction cylinder, and the tube inside of the test tube wall is tightly attached to the heat insulation plate.
Preferably, the cardboard outside is the rectangle, and the cardboard is inside closely to laminate with the test tube outer wall.
Preferably, the extracting plate is made of pve material, and the extracting plate is fixedly connected with the sealing ring.
Preferably, the diameter size of the extraction plate is consistent with the diameter size of the sealing ring, and the extraction plate and the test tube are mutually matched to form a sliding structure.
Preferably, the device is characterized in that scale values are arranged on the front side and the rear side of the pull tube, and the outer wall of the pull tube is consistent with the inner wall of the groove in size.
Preferably, the diameter of the pulling pipe is half of the diameter of the sealing ring, and the extraction plate, the sealing ring and the pulling pipe are positioned on the same vertical line.
Preferably, the leak-proof piece, the locking piece are tightly attached to the inside of the bottom plate and the outside of the test tube, and the leak-proof piece and the locking piece are fixedly connected.
The working principle and the beneficial effects of the utility model are as follows:
1. according to the utility model, through the arrangement of the suspension extracting device, the suspension extracting device consists of an extracting plate, a sealing ring, a pull tube, an exhaust port, a main body and the like, wherein the extracting plate is used for sealing the inside of a test tube to prevent air circulation, the sealing ring is used for sealing the mouth of the test tube to prevent pollution after reagent is taken out, air in the pull tube is extracted through a button on the main body, and the air in the pull tube is discharged from the exhaust port, so that the pull tube is fixed on the extracting plate to realize the pulling effect, and the scale values marked on the pull tube can be used for extracting the reagent according to requirements.
2. According to the sealing device, after reagent extraction is completed, the bottom plate is placed at the bottom of the test tube, the sealing structure is achieved by the aid of the structure that the fixing piece and the clamping groove are mutually embedded, leakage of reagents in the test tube can be effectively prevented by the aid of the leakage-proof piece on the periphery of the clamping groove, and the leakage-proof piece is fixed at the bottom of the test tube by the aid of the fixing structure of the locking piece and the leakage-proof piece.
Drawings
The utility model will be described in further detail with reference to the drawings and the detailed description.
FIG. 1 is a schematic view showing the overall structure of a reagent extraction device for flow cytometry according to one embodiment of the present utility model;
FIG. 2 is a schematic view showing a main frame structure of a reagent extraction device for flow cytometry according to one embodiment of the present utility model;
FIG. 3 is a schematic view showing a structure of a suspension extracting device of a flow cytometry reagent extracting apparatus according to one embodiment of the present utility model;
FIG. 4 is a schematic diagram showing a sealing device of a reagent extraction device for flow cytometry according to one embodiment of the present utility model;
in the figure: 1. a test tube; 101. a clamping plate; 102. a heat insulating plate; 103. a fixing member; 2. an extraction plate; 201. sealing ring; 202. pulling a tube; 203. a scale value; 204. a groove; 205. a button; 206. an exhaust port; 207. a main body; 3. a bottom plate; 301. a leakage preventing member; 302. a clamping groove; 303. a locking member.
Detailed Description
The technical solutions of the embodiments of the present utility model will be clearly and completely described below in conjunction with the embodiments of the present utility model, and it is apparent that the described embodiments are only some embodiments of the present utility model, not all embodiments. All other embodiments, which can be made by one of ordinary skill in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Example 1
As shown in fig. 1 to 4, in the present embodiment, a flow cytometry reagent extraction device includes: the test tube 1, the periphery of the test tube 1 is provided with a clamping plate 101, the outer part of the clamping plate 101 is rectangular, the inside of the clamping plate 101 is tightly attached to the outer wall of the test tube 1, the outer structure of the test tube 1 is an adduction cylinder, the inside of the wall of the test tube 1 is tightly attached to a heat insulation plate 102, a suspension extracting device is arranged above the test tube 1, the inside of the test tube 1 is provided with a heat insulation plate 102, the bottom of the test tube 1 is provided with a fixing piece 103, and a sealing device is arranged below the fixing piece 103;
in this embodiment, the bottom structure of the test tube 1 is a contracted cylinder, the heat insulation board 102 is located in the test tube 1, which plays a role of isolating the external temperature, and the clamping board 101 facilitates the test tube 1 to be placed on the rack.
The sealing device comprises: the bottom plate 3 is arranged below the fixing piece 103, the clamping groove 302 is formed in the periphery of the bottom plate 3, the leakage-proof piece 301 is arranged on the outer layer of the clamping groove 302, the locking piece 303 is arranged at the upper end of the leakage-proof piece 301, the leakage-proof piece 301 and the locking piece 303 are tightly attached to the inside of the bottom plate 3 and the outside of the test tube 1, and the leakage-proof piece 301 and the locking piece 303 are fixedly connected.
In this embodiment, the fixing member 103 and the clamping groove 302 are engaged with each other to prevent leakage of the reagent, the diameter of the bottom plate 3 is identical to that of the bottom of the test tube 1, and the locking member 303 is fixedly connected to the upper end of the leakage preventing member 301, so that the leakage preventing member 301 is fixed below the test tube 1.
Embodiment two:
as shown in fig. 1 to 3, based on the same concept as that of embodiment 1 described above, this embodiment also proposes that the suspension extraction device includes: the drawing tube is characterized in that an extraction plate 2 is arranged on the inner wall of the test tube 1, a sealing ring 201 is arranged on the periphery of the extraction plate 2, the extraction plate 2 is made of pve materials, the extraction plate 2 is fixedly connected with the sealing ring 201, the diameter size of the extraction plate 2 is consistent with that of the sealing ring 201, the extraction plate 2 and the test tube 1 are mutually matched to form a sliding structure, a drawing tube 202 is arranged above the extraction plate 2, scale values 203 are carved on the outer wall of the drawing tube 202, scale values 203 are respectively arranged on the front side and the rear side of the drawing tube 202, the outer wall of the drawing tube 202 is consistent with the inner wall of the groove 204, the diameter of the drawing tube 202 is half of that of the sealing ring 201, the extraction plate 2, the sealing ring 201 and the drawing tube 202 are positioned on the same vertical line, a main body 207 is arranged at the upper end of the drawing tube 202, the drawing tube 202 is connected with the groove 204, a button 205 is arranged on the left side below the main body 207, and an exhaust port 206 is arranged on the right side of the main body 207;
in this embodiment, the drawing plate 2 is mounted on the inner wall of the test tube 1, the drawing plate 2 and the test tube 1 are mutually matched to form a sliding structure, the measurement of the reagent can be controlled by the scale value 203 on the drawing tube 202, the air outlet 206 on one side of the main body 207 is used for drawing out the air of the drawing tube 202, so that the drawing tube 202 is fixed on the drawing plate 2, and the cuboid structure of the main body 207 can prevent the test tube 1 from rolling and damaging.
Working principle: firstly, the front end of the pull tube 202 is placed with the drawing plate 2 and the sealing ring 201, air in the pull tube 202 is drawn out through a button 205 at the left side of the main body 207 and discharged from the air outlet 206, the vacuum in the pull tube 202 can fix the air on the drawing plate 2, the drawing plate 2 is placed in the test tube 1, the drawing scale 203 of the pull tube 202 controls the drawing metering, the test tube 1 is placed in the reagent, the main body 207 is pulled to drive the pull tube 202 to lift up and draw the reagent, after the completion, the bottom plate 3 is fixed at the bottom of the test tube 1 through a jogged structure of the clamping groove 302 and the fixing piece 103, the leak-proof piece 301 wraps the bottom of the test tube 1 to prevent the reagent from leaking out, the locking piece 303 at the upper end is fixed at the bottom of the test tube 1, the button 205 on the main body 207 is used for sucking the air from the air outlet 206, the pull tube 202 is filled with air, the fixing with the drawing plate 2 is released, the rubber material of the sealing ring 201 is turned outwards to fix the top of the test tube 1 and the drawing plate 2 to prevent impurities from entering, meanwhile, the temperature and other factors affecting the reagent can be effectively prevented by 102 in the test tube 1, and the effect of the test tube 1 is finally prevented from affecting factors such as the hand, and the effect of the heat insulating plate is hung on the test tube 101.
The foregoing description of the preferred embodiments of the utility model is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the utility model.
Claims (8)
1. A flow cytometry reagent extraction apparatus, comprising: the device comprises a test tube (1), wherein a clamping plate (101) is arranged on the periphery of the test tube (1), a suspension extraction device is arranged above the test tube (1), a heat insulation plate (102) is arranged inside the test tube (1), a fixing piece (103) is arranged at the bottom of the test tube (1), and a sealing device is arranged below the fixing piece (103);
the suspension extraction device comprises: the automatic test tube drawing device is characterized in that an extraction plate (2) is arranged on the inner wall of the test tube (1), a sealing ring (201) is arranged on the periphery of the extraction plate (2), a drawing tube (202) is arranged above the extraction plate (2), scale values (203) are carved on the outer wall of the drawing tube (202), a main body (207) is arranged at the upper end of the drawing tube (202), the drawing tube (202) is connected with a groove (204), a button (205) is arranged on the left side below the main body (207), and an exhaust port (206) is arranged on the right side of the main body (207);
the sealing device comprises: the anti-leakage device is characterized in that a bottom plate (3) is arranged below the fixing piece (103), a clamping groove (302) is formed in the periphery of the bottom plate (3), a leakage-proof piece (301) is arranged on the outer layer of the clamping groove (302), and a locking piece (303) is arranged at the upper end of the leakage-proof piece (301).
2. The reagent extraction device for flow cytometry according to claim 1, wherein the outer structure of the test tube (1) is an adduction cylinder, and the inside of the wall tube of the test tube (1) is tightly attached to the heat insulation plate (102).
3. The flow cytometry reagent extraction device according to claim 1, wherein the outer portion of the clamping plate (101) is rectangular, and the inner portion of the clamping plate (101) is tightly attached to the outer wall of the test tube (1).
4. The device according to claim 1, wherein the extraction plate (2) is made of pve material, and the extraction plate (2) is fixedly connected with the sealing ring (201).
5. A flow cytometry reagent extraction device according to claim 3, wherein the diameter of the extraction plate (2) is identical to the diameter of the sealing ring (201), and the extraction plate (2) and the test tube (1) cooperate to form a sliding structure.
6. The device according to claim 5, wherein the pull tube (202) has graduation values (203) on both front and rear sides, and the outer wall of the pull tube (202) is consistent with the inner wall of the groove (204).
7. The device according to claim 4, wherein the diameter of the pull tube (202) is half of the diameter of the sealing ring (201), and the extraction plate (2), the sealing ring (201) and the pull tube (202) are on the same vertical line.
8. A flow cytometry reagent extraction apparatus according to claim 3, wherein the leakage preventing member (301), the locking member (303) and the bottom plate (3) are tightly attached to the outside of the test tube (1), and the leakage preventing member (301) and the locking member (303) are fixedly connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321550982.2U CN220405677U (en) | 2023-06-19 | 2023-06-19 | Flow cytometry reagent extraction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321550982.2U CN220405677U (en) | 2023-06-19 | 2023-06-19 | Flow cytometry reagent extraction device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220405677U true CN220405677U (en) | 2024-01-30 |
Family
ID=89656218
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321550982.2U Active CN220405677U (en) | 2023-06-19 | 2023-06-19 | Flow cytometry reagent extraction device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220405677U (en) |
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2023
- 2023-06-19 CN CN202321550982.2U patent/CN220405677U/en active Active
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