CN211652292U - Portable reticulocyte dyeing device - Google Patents
Portable reticulocyte dyeing device Download PDFInfo
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- CN211652292U CN211652292U CN202020247034.1U CN202020247034U CN211652292U CN 211652292 U CN211652292 U CN 211652292U CN 202020247034 U CN202020247034 U CN 202020247034U CN 211652292 U CN211652292 U CN 211652292U
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- negative pressure
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Abstract
The utility model provides a portable reticulocyte dyeing device, which comprises a push-pull rod, a positioning block and a negative pressure tube, wherein the negative pressure tube is a fixing cap, a negative pressure tube body and a branch port in sequence from top to bottom; the fixing cap is fixedly arranged at the top of the negative pressure pipe body; the push-pull rod is arranged in the negative pressure pipe body through the fixing cap; the push-pull rod can move up and down in the negative pressure pipe body; the positioning block is arranged outside the negative pressure tube body; the branch port is arranged at the end part of the negative pressure pipe body. The device is in the reticulocyte dyeing process, not only can effectively avoid the pollution of blood sample, and can effectively improve experimental efficiency.
Description
Technical Field
The utility model relates to a biological test equipment especially relates to a portable net tissue erythrocyte staining device.
Background
Reticulocytes are not fully mature red blood cells, and their value in the peripheral blood reflects the production function of bone marrow erythrocytes, thus being of great significance for diagnosis of hematological disorders and observation of therapeutic responses. However, the staining of reticulocytes requires vital staining, since reticulocytes continue to develop after being isolated, i.e., lack of timely detection is likely to result in a decrease in the positive rate.
Currently, in clinical laboratories, staining methods commonly used include a slide method and a test tube method. For the slide method, as the reticulocytes need to be dyed for about half an hour, the dyeing solution dyed by the slide method is evaporated to form sediments and is finally eliminated gradually. For the test tube method, a more advocated method is adopted at present, and the specific process is as follows: smears were prepared from the stained solution by adding the stain to the tube, adding fresh whole blood, mixing well and incubating at 37 ℃ for 30 min. Although the test tube method is a more advocating method, the method has more steps, instruments needed to be used in the experiment teaching relate to a test tube, a sample adding gun head, an anticoagulation tube and the like, a student needs to use reagents and partial instruments in turn in the operation process, and even partial instruments cannot be reused due to poor cleaning.
SUMMERY OF THE UTILITY MODEL
Not enough to prior art, the utility model provides a portable net red blood cell dyeing apparatus, it is at net red blood cell dyeing in-process, not only can effectively avoid the pollution of blood sample, and can effectively improve experimental efficiency.
In order to solve the technical problem, the utility model provides a following technical scheme:
on one hand, the utility model provides a portable reticulocyte dyeing device, which comprises a push-pull rod, a positioning block and a negative pressure tube,
the negative pressure pipe is provided with a fixing cap, a negative pressure pipe body and a branch port in sequence from top to bottom;
the fixing cap is fixedly arranged at the top of the negative pressure pipe body;
the push-pull rod is arranged in the negative pressure pipe body through the fixing cap; the push-pull rod can move up and down in the negative pressure pipe body;
the push-pull rod comprises a push-pull handle, a push-pull column and a push-pull plate which are fixedly arranged in sequence; a positioning block is arranged between the push-pull handle and the negative pressure pipe;
the branch port is arranged at the end part of the negative pressure pipe body.
Furthermore, the middle part of the fixing cap is provided with a mounting hole;
the push-pull column is provided with an external thread; an internal thread matched with the external thread is arranged in the positioning block;
the positioning block is used for fixing the specific position of the push-pull plate in the negative pressure pipe.
Further, the outer wall of the negative pressure pipe body is provided with scales.
Further, the push-pull handle is provided with anti-skidding lines.
Further, the inner diameter of the mounting hole of the fixing cap is smaller than the outer diameter of the positioning block and larger than the outer diameter of the push-pull rod.
Further, the diameter of the push-pull plate is matched with the inner diameter of the negative pressure pipe body.
Furthermore, the branch port is provided with a first outlet and a second outlet; arc-shaped protrusions are arranged on the first outlet and the second outlet; the outlet I and the outlet II are sleeved with thick rubber pipes, and water stopping clamps are arranged on the thick rubber pipes.
Further, the first outlet is connected with a negative pressure air extraction device through a pipeline.
Advantageous effects
Compared with the prior art, the utility model, following beneficial effect has:
the utility model provides a portable net red blood cell staining device has following advantage:
through the arrangement of the negative pressure tube and the push-pull rod, the volume of the collected blood sample in the negative pressure tube can be limited, and the solution amount in the operation process can be measured conveniently;
the position of the push-pull rod is limited under negative pressure under the limiting action of the positioning block and the push-pull rod, so that the pressure in the negative pressure pipe is ensured;
by using the device of the utility model, the problem of pollution caused by the contact of the collected blood sample with the outside can be solved; utilize the utility model discloses a device, the volume proportion of steerable dyeing liquid and blood sample can be saved and processes such as anticoagulant need be added at the dyeing in-process at the meshwork erythrocyte, and a preparation pipe can supply many people to use simultaneously, no matter use in clinical one-line still in the teaching can all save the use of experimental devices such as application of sample rifle head, anti-freezing pipe, test tube, save trouble laborsaving saving time.
Drawings
FIG. 1 is a schematic view showing the structure of the apparatus shown in example 1; wherein, the device is filled with a dyeing liquid 10;
FIG. 2 is a schematic view showing the structure of the apparatus according to embodiment 1; wherein the device contains reticulocytes which have been stained;
FIG. 3 is a schematic view of the structure of the orifice.
Detailed Description
The present invention will be described in further detail with reference to specific embodiments, but it should not be construed that the scope of the subject matter of the present invention is limited to the following examples.
The process equipment or devices not specifically noted in the following examples are conventional in the art; all reagents are commercially available.
Example 1
The utility model provides a portable reticulocyte dyeing device, which comprises a push-pull rod 1 and a negative pressure tube,
the push-pull rod 1 comprises a push-pull handle 11, a push-pull column 12 and a push-pull plate 8 which are fixedly arranged in sequence;
the push-pull handle 11 is provided with anti-skid grains, so that the rotation stability of the push-pull rod in the up-and-down moving process is facilitated;
the push-pull column 12 is provided with an external thread;
a positioning block 4 is arranged on the push-pull column 12, and an internal thread matched with the external thread is arranged in the positioning block 4;
the positioning block 4 is arranged between the push-pull handle 11 and the negative pressure pipe, that is, the positioning block 4 is arranged on the push-pull column 12 outside the negative pressure pipe; the positioning block 4 is used for fixing the specific position of the push-pull plate 8 in the negative pressure pipe, namely, for locking the space in the negative pressure pipe;
the negative pressure tube is sequentially provided with a fixing cap 2, a negative pressure tube body 3 and a branch port 5 from top to bottom;
scales 9 are arranged on the outer wall of the negative pressure tube body 3, so that the measurement of injected and discharged liquid is facilitated;
the fixing cap 2 is fixedly arranged at the top of the negative pressure tube body 3, and a mounting hole is formed in the middle of the fixing cap 2;
the push-pull rod 1 is arranged in the negative pressure pipe body 3 through the fixing cap 2; the push-pull rod can move up and down in the negative pressure pipe body 3, and the position of the push-pull plate 8 in the negative pressure pipe body is limited by the positioning block 4; the inner diameter of the mounting hole of the fixing cap is smaller than the outer diameter of the positioning block 4 and larger than the outer diameter of the push-pull rod 1, so that the push-pull rod can move up and down and the positioning block 4 can be fixed to the position of the push-pull rod 1;
the diameter of the push-pull plate 8 is matched with the inner diameter of the negative pressure pipe body 3, so that the sealing performance of the negative pressure pipe body 3 is kept;
the branch port 5 is arranged at the end part of the negative pressure pipe body 3; the branch port 5 is provided with a first outlet and a second outlet; the first outlet and the second outlet are provided with arc-shaped protrusions 51 (shown in figure 3) which are beneficial to sealing the thick rubber pipe 6; thick rubber pipes 6 are sleeved on the first outlet and the second outlet, and water stopping clamps 7 are arranged on the thick rubber pipes 6; the first outlet is connected with a negative pressure air extraction device through a pipeline; the outlet is used for injecting staining solution and blood samples;
two branch ears can be arranged outside the negative pressure tube body 3, so that the subsequent devices can be placed conveniently.
The specific experimental process is as follows:
placing the device of the embodiment upside down, opening the two water stop clamps 7 at the branch openings 5, moving the push-pull rod 1 up and down, ensuring that the lower end of the positioning block 4 is close to the fixing cap according to the experiment requirement, and moving the position of the push-pull plate 8 to the scale of the required solution volume; installing a water stop clamp 7 to close the branch port 5; connecting the first outlet with a negative pressure air extraction device through a pipeline; 1/2 volumes of staining solution were injected through outlet two using a syringe; opening the water stop clamp at the first outlet, connecting a power supply of the negative pressure air exhaust device, and vacuumizing the negative pressure tube body; after the staining solution starts to bubble, the water stop clamp is closed, the power supply of the negative pressure air exhaust device is turned over, and the state in the negative pressure tube body is as shown in figure 1.
One end of the blood taking needle is communicated with the device through a thick rubber tube at the second outlet, the other end of the blood taking needle is used for taking blood, and when the volume of the blood sample to be injected and the volume of the staining solution are 1:1, the blood taking is finished; the device is turned back and forth to mix the staining solution and the blood sample, and then the device is placed in water bath with the temperature of 37 ℃ for staining reaction; the state in the negative pressure tube body after completion of the dyeing reaction is shown in FIG. 2.
After dyeing is finished, the water stop clamp of the second outlet is opened, the push-pull rod is moved slowly after the fixed block is adjusted to be upward, so that the specimen is extruded, and the specimen can be used for microscopic examination after smear making.
It is to be understood that the above embodiments are merely exemplary embodiments that have been employed to illustrate the principles of the present invention, and that the present invention is not limited thereto. It will be apparent to those skilled in the art that various modifications and improvements can be made without departing from the spirit and substance of the invention, and these modifications and improvements are also considered to be included in the scope of the invention.
Claims (6)
1. A portable reticulocyte dyeing device is characterized by comprising a push-pull rod, a positioning block and a negative pressure tube,
the negative pressure pipe is provided with a fixing cap, a negative pressure pipe body and a branch port in sequence from top to bottom;
the fixing cap is fixedly arranged at the top of the negative pressure pipe body;
the push-pull rod is arranged in the negative pressure pipe body through the fixing cap; the push-pull rod can move up and down in the negative pressure pipe body;
the push-pull rod comprises a push-pull handle, a push-pull column and a push-pull plate which are fixedly arranged in sequence; a positioning block is arranged between the push-pull handle and the negative pressure pipe;
the branch port is arranged at the end part of the negative pressure pipe body.
2. The portable reticulocyte dyeing device of claim 1,
the middle part of the fixing cap is provided with a mounting hole;
the push-pull column is provided with an external thread; an internal thread matched with the external thread is arranged in the positioning block;
the positioning block is used for fixing the specific position of the push-pull plate in the negative pressure pipe.
3. The portable reticulocyte dyeing device according to claim 2, characterized in that the outer wall of the negative pressure tube body is provided with graduations;
the push-pull handle is provided with anti-skidding lines.
4. The portable reticulocyte dyeing device of claim 2, wherein the inner diameter of the mounting hole of the fixing cap is smaller than the outer diameter of the positioning block and larger than the outer diameter of the push-pull rod; the diameter of the push-pull plate is matched with the inner diameter of the negative pressure pipe body.
5. The portable reticulocyte dyeing device according to claim 1, characterized in that the branch port is provided with a first outlet and a second outlet; arc-shaped protrusions are arranged on the first outlet and the second outlet; the outlet I and the outlet II are sleeved with thick rubber pipes, and water stopping clamps are arranged on the thick rubber pipes.
6. The portable reticulocyte dyeing device of claim 5, wherein the first outlet is connected with a negative pressure air suction device through a pipeline.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020247034.1U CN211652292U (en) | 2020-03-03 | 2020-03-03 | Portable reticulocyte dyeing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020247034.1U CN211652292U (en) | 2020-03-03 | 2020-03-03 | Portable reticulocyte dyeing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211652292U true CN211652292U (en) | 2020-10-09 |
Family
ID=72686473
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020247034.1U Expired - Fee Related CN211652292U (en) | 2020-03-03 | 2020-03-03 | Portable reticulocyte dyeing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211652292U (en) |
-
2020
- 2020-03-03 CN CN202020247034.1U patent/CN211652292U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201009 Termination date: 20210303 |
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| CF01 | Termination of patent right due to non-payment of annual fee |