CN220283954U - Integrated device for efficient extraction, separation and preparation of collagen aggregate - Google Patents
Integrated device for efficient extraction, separation and preparation of collagen aggregate Download PDFInfo
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- CN220283954U CN220283954U CN202321684121.3U CN202321684121U CN220283954U CN 220283954 U CN220283954 U CN 220283954U CN 202321684121 U CN202321684121 U CN 202321684121U CN 220283954 U CN220283954 U CN 220283954U
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- 102000008186 Collagen Human genes 0.000 title claims abstract description 25
- 108010035532 Collagen Proteins 0.000 title claims abstract description 25
- 229920001436 collagen Polymers 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims description 16
- 238000000926 separation method Methods 0.000 title claims description 16
- 238000000605 extraction Methods 0.000 title claims description 15
- 238000003756 stirring Methods 0.000 claims abstract description 32
- 238000007789 sealing Methods 0.000 claims abstract description 25
- 239000000126 substance Substances 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 10
- 239000002002 slurry Substances 0.000 description 8
- 238000001914 filtration Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 230000014509 gene expression Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 241001330002 Bambuseae Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000003828 vacuum filtration Methods 0.000 description 1
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- Physical Or Chemical Processes And Apparatus (AREA)
Abstract
The utility model discloses an integrated device for efficiently extracting, separating and preparing collagen aggregates, and relates to the technical field of organic chemical industry. The integrated device for efficiently extracting, separating and preparing the collagen aggregate comprises a device body, wherein a sealing cover is arranged on the device body, a closed reaction cavity is formed between the sealing cover and the device body, a reaction container is arranged in the reaction cavity, a centrifugal machine for centrifuging the reaction container is arranged in the device body, and a refrigerator for providing a low-temperature environment is also arranged in the device body; the reaction chamber is internally provided with a stirring mechanism for stirring the inside of the reaction container, the device body is internally provided with a filter chamber, the filter chamber is communicated with the reaction chamber through a filter funnel, and a collecting container is arranged below the filter funnel; a vacuum mechanism is also included for providing a vacuum environment for the reaction chamber and the filter chamber.
Description
Technical Field
The utility model relates to the technical field of organic chemical industry, in particular to an integrated device for efficiently extracting, separating and preparing collagen aggregates.
Background
The extraction of collagen aggregates from animals (such as cows, etc.) is receiving increasing attention, and because collagen aggregates have the characteristics and advantages of high strength, flexibility and porosity compared with common collagen, collagen aggregates are being gradually used for constructing biological materials for repairing injury of organisms, which is a research hot spot and focus in the field at present.
The method is characterized in that the method comprises the steps of extracting collagen aggregates in a research and experiment process, including soaking, homogenizing, stirring, centrifuging, vacuumizing, filtering, freeze-drying and the like, wherein the environment temperature is required to be kept at 2-8 ℃ (the freeze-drying is required to be at a lower temperature), the existing instrument only has a certain function, multiple devices are required to be cooperatively completed, the problem that intermediate links are not connected up is often caused, and the extraction working efficiency is influenced.
Disclosure of Invention
Therefore, the embodiment of the utility model provides an integrated device for efficiently extracting, separating and preparing collagen aggregates, which solves the problems existing in the prior art.
In order to achieve the above object, the embodiment of the present utility model provides the following technical solutions:
the integrated device comprises a device body, wherein a sealing cover is arranged on the device body, a closed reaction cavity is formed between the sealing cover and the device body, a reaction container is arranged in the reaction cavity, a centrifugal machine for centrifuging the reaction container is arranged in the device body, and a refrigerator for providing a low-temperature environment is also arranged in the device body;
the reaction chamber is internally provided with a stirring mechanism for stirring the inside of the reaction container, the device body is internally provided with a filter chamber, the filter chamber is communicated with the reaction chamber through a filter funnel, and a collecting container is arranged below the filter funnel;
a vacuum mechanism is also included for providing a vacuum environment for the reaction chamber and the filter chamber.
Optionally, a support is arranged at the top of the device body and used for fixing the reaction container, and the reaction support is connected with the centrifuge.
Optionally, the vacuum mechanism includes the vacuum pump that sets up in device body one side, the vacuum pump passes through first pipeline and the inside UNICOM of reaction chamber, the vacuum pump passes through second pipeline and the inside section of thick bamboo of filter chamber, all be provided with the valve on first pipeline and the second pipeline.
Optionally, the stirring mechanism comprises an L-shaped supporting rod, one end of the L-shaped supporting rod is fixedly connected with the device body, the other end of the L-shaped supporting rod is arranged above the reaction container, one end of the L-shaped supporting rod above the reaction container is fixedly connected with a motor, the output end of the motor is fixedly connected with a stirring rod, and the stirring rod is arranged inside the reaction container.
Optionally, a first window for opening the reaction cavity is arranged on one side of the sealing cover, and the first window and the sealing cover are arranged in a sealing way.
Optionally, a second window for opening the filter cavity is arranged on one side of the device body, and the second window and the device body are arranged in a sealing way.
Optionally, the valve is an electromagnetic valve.
Optionally, a control mechanism is arranged on one side of the device body, and the vacuum pump, the electromagnetic valve, the centrifugal machine, the refrigerator and the motor are all electrically connected with the control mechanism.
The utility model has at least the following beneficial effects:
according to the utility model, the collagen aggregate is soaked by the reaction container arranged on the device body, then stirred and homogenized by the stirring mechanism, the centrifugal machine is subjected to centrifugal operation, the vacuum mechanism provides a required vacuum filtration environment, the refrigerator improves a required low-temperature environment, various high-efficiency instruments and equipment are connected in series, each link is ensured to be in the low-temperature environment, and the high-efficiency extraction, separation and preparation of the collagen aggregate are ensured, so that the working efficiency is improved.
Drawings
In order to more clearly illustrate the prior art and the present utility model, the drawings used in the description of the prior art and the embodiments of the present utility model will be briefly described. It will be apparent to those skilled in the art that the drawings in the following description are merely exemplary and that other drawings may be derived from the drawings provided without the inventive effort to those skilled in the art.
The structures, proportions, sizes, etc. shown in the present specification are shown only for the purposes of illustration and description, and are not intended to limit the scope of the utility model, which is defined by the claims, for example, modifications, variations in proportions, or otherwise, used in the practice of the utility model, which are particularly adapted to specific environments without departing from the spirit and scope of the utility model.
Fig. 1 is a schematic diagram of an internal structure of an embodiment of the present utility model.
Reference numerals illustrate:
1. a device body; 2. a sealing cover; 3. a reaction chamber; 4. a reaction vessel; 5. a centrifuge; 6. a refrigerating machine; 7. a filter chamber; 8. a filter funnel; 9. a collection container; 10. a bracket; 11. a vacuum pump; 12. a first pipe; 13. a second pipe; 14. a valve; 15. an L-shaped supporting rod; 16. a motor; 17. a stirring rod; 18. a first window; 19. and a second window.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the present application will be further described in detail with reference to the accompanying drawings and examples. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the present application.
In the description of the present utility model, unless otherwise indicated, the meaning of "a plurality" is two or more. The terms "first," "second," "third," "fourth," and the like in the description and in the claims of the utility model, if any, are intended to distinguish between the referenced objects. For schemes with time sequence flows, such term expressions are not necessarily to be understood as describing a specific order or sequence, nor are such term expressions to distinguish between importance levels, positional relationships, etc. for schemes with device structures.
Furthermore, the terms "comprises," "comprising," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements that are expressly listed but may include other steps or elements not expressly listed but inherent to such process, method, article, or apparatus or steps or elements that may be added based on a further optimization of the inventive concept.
As shown in fig. 1, the integrated device for efficient extraction, separation and preparation of collagen aggregates disclosed by the utility model comprises a device body 1, wherein a sealing cover 2 is arranged on the device body 1, a sealed reaction cavity 3 is formed between the sealing cover 2 and the device body 1, a reaction container 4 is arranged in the reaction cavity 3, a centrifugal machine 5 for centrifuging the reaction container 4 is arranged in the device body 1, and a refrigerator 6 for providing a low-temperature environment is also arranged in the device body 1;
a stirring mechanism is arranged in the reaction cavity 3 to stir the interior of the reaction container 4, a filter cavity 7 is arranged in the device body 1, the filter cavity 7 is communicated with the reaction cavity 3 through a filter funnel 8, and a collecting container 9 is arranged below the filter funnel 8;
a vacuum mechanism is also included to provide a vacuum environment for reaction chamber 3 and filter chamber 7.
The sealing cover 2 is a transparent glass cover, so that the state of the internal reaction container 4 can be conveniently observed; the sealing cover 2 is fixedly connected with the device body 1, a sealing gasket is arranged at the joint for sealing, the tightness of the internal reaction cavity 3 is ensured, the reaction container 4 is used for placing collagen aggregate slurry to be prepared, stirring and homogenizing are carried out through a stirring mechanism, centrifugal separation is carried out through a centrifugal machine 5, and the separated solution is poured into a filter funnel 8 for filtering and is collected through a collecting container 9; in the preparation process, the vacuum mechanism and the refrigerator 6 provide vacuum and low-temperature environment required by preparation;
the filter funnel 8 adopts the existing vacuum filter, and communication between the reaction cavity 3 and the filter cavity 7 in the vacuumizing process is not needed to be considered.
In order to prevent the solution from spilling out during the stirring process, an end cover is arranged at the top of the reaction vessel 4, and the stirring mechanism penetrates through the end cover and stretches into the reaction vessel 4.
In the preparation process, the vacuum mechanism sequentially performs vacuumizing operation on the reaction cavity 3 and the filter cavity 7.
The top of the device body 1 is provided with a bracket 10 for fixing the reaction vessel 4, and the reaction bracket 10 is connected with the centrifuge 5.
The connection between the support 10 and the reaction vessel 4 is detachable, when the reaction vessel 4 needs to be operated, the reaction vessel 4 is detached, the solution in the reaction vessel 4 is poured into the filter funnel 8, and the reaction vessel 4 is fixed on the support 10 during separation and stirring, so that the stability of the reaction vessel 4 is maintained.
The vacuum mechanism comprises a vacuum pump 11 arranged on one side of the device body 1, the vacuum pump 11 is communicated with the inside of the reaction cavity 3 through a first pipeline 12, the vacuum pump 11 is in cylinder connection with the inside of the filter cavity 7 through a second pipeline 13, and valves 14 are arranged on the first pipeline 12 and the second pipeline 13.
The vacuum pump 11 is communicated with the reaction cavity 3 through a first pipeline 12, and the reaction cavity 3 is vacuumized through the vacuum pump 11, so that the vacuum environment required by the reaction is ensured; the vacuum pump 11 is communicated with the filter cavity 7 through the second pipeline 13, and the filter cavity 7 is vacuumized through the vacuum pump 11, so that a pressure difference is formed between the filter cavity 7 and one side of the accommodating cavity, and the filtering efficiency of the filter funnel 8 is improved;
valves 14 are provided on both the first conduit 12 and the second conduit 13 to ensure that only one chamber is evacuated at a time.
The valve 14 is a solenoid valve.
The stirring mechanism comprises an L-shaped supporting rod 15, one end of the L-shaped supporting rod 15 is fixedly connected with the device body 1, the other end of the L-shaped supporting rod 15 is arranged above the reaction container 4, one end of the L-shaped supporting rod 15 above the reaction container 4 is fixedly connected with a motor 16, the output end of the motor 16 is fixedly connected with a stirring rod 17, and the stirring rod 17 is arranged inside the reaction container 4.
The L-shaped supporting rod 15 is arranged on one side of the top of the device body 1, one end of the horizontal part of the L-shaped supporting rod 15 is arranged above the reaction vessel 4, the bottom of the horizontal part of the L-shaped supporting rod 15 is connected with the motor 16, the stirring rod 17 is fixed at the output end of the motor 16, the stirring rod 17 is arranged in the reaction vessel 4, and the stirring rod 17 is driven to rotate by the motor 16, so that stirring of the solution in the reaction vessel 4 is realized, and the solution in the reaction vessel is uniformly mixed.
The above-mentioned fixed connection among the L-shaped supporting rod 15, the motor 16 and the stirring rod 17 is detachable fixed connection, and can be disassembled and assembled.
A first window 18 for opening the reaction cavity 3 is arranged on one side of the sealing cover 2, and the first window 18 and the sealing cover 2 are arranged in a sealing way.
A second window 19 for opening the filter cavity 7 is arranged on one side of the device body 1, and the second window 19 and the device body 1 are arranged in a sealing way.
The first window 18 and the second window 19 are provided, after the homogenization and separation are completed, the first window 18 is opened, the reaction vessel 4 is removed from the support 10, the slurry in the reaction vessel is poured into the filter funnel 8, the second window 19 is opened after the filtration is completed, and the collection vessel 9 is taken out.
The device comprises a device body 1, wherein a control mechanism is arranged on one side of the device body, and a vacuum pump 11, an electromagnetic valve, a centrifugal machine 5, a refrigerator 6 and a motor 16 are all electrically connected with the control mechanism.
Through setting up control mechanism, conveniently operate.
The utility model designs a high-efficiency integrated instrument and equipment which connects a homogenizer, a stirrer, a centrifugal machine 5, a vacuum filter and a freeze dryer in series, ensures that all links are in a low-temperature environment, can ensure more efficient extraction, separation and preparation of collagen aggregates, and saves the laboratory space to a certain extent. In addition, each part that this high-efficient instrument equipment contained also can be respectively alone to accomplish other experiments and prepare other similar chemical products to satisfy other daily experimental needs in laboratory.
Operating procedure
1. Opening the first window 18, fixing the reaction vessel 4 on the bracket 10, pouring the slurry of the collagen aggregate into the reaction vessel 4, closing the first window 18, standing and soaking;
2. the refrigerator 6 is controlled to work through the control mechanism;
3. the electromagnetic valve on the first pipeline 12 is controlled to be opened through the control mechanism, then the vacuum pump 11 is controlled to work, the reaction cavity 3 is vacuumized, and the electromagnetic valve and the vacuum pump 11 are closed after the vacuumizing is finished;
4. the motor 16 is controlled by the control mechanism to work so as to drive the stirring rod 17 to rotate and stir the slurry in the reaction vessel 4;
5. the centrifuge 5 is controlled to work through the control mechanism, the bracket 10 and the reaction vessel 4 are driven to rotate, and the slurry in the reaction vessel 4 is centrifugally separated;
6. the electromagnetic valve on the second pipeline 13 is controlled to be opened through the control mechanism, the vacuum pump 11 is controlled to work, the filter cavity 7 is vacuumized, and the electromagnetic valve and the vacuum pump 11 are closed after the vacuumizing is finished;
6. opening a first window 18, detaching the reaction vessel 4, pouring the slurry in the reaction vessel into a filter funnel 8 for filtering, and collecting the filtered slurry by a collecting vessel 9;
7. the second window 19 is opened, the collection vessel 9 is withdrawn, and the slurry therein is collected.
The above specific embodiments may be combined with each other and some embodiments may not be repeated for the same or similar concepts or processes.
Any combination of the technical features of the above embodiments may be performed (as long as there is no contradiction between the combination of the technical features), and for brevity of description, all of the possible combinations of the technical features of the above embodiments are not described; these examples, which are not explicitly written, should also be considered as being within the scope of the present description.
The utility model has been described above with particularity and detail in connection with general description and specific embodiments. It should be noted that it is obvious that several variations and modifications can be made to these specific embodiments without departing from the spirit of the present utility model, which are all within the scope of protection of the present application. Accordingly, the scope of protection of the present application is to be determined by the claims appended hereto.
Claims (8)
1. The utility model provides a collagen aggregate high-efficient extraction separation preparation integrated device which characterized in that: the device comprises a device body (1), wherein a sealing cover (2) is arranged on the device body (1), a closed reaction cavity (3) is formed between the sealing cover (2) and the device body (1), a reaction container (4) is arranged in the reaction cavity (3), a centrifugal machine (5) for centrifuging the reaction container (4) is arranged in the device body (1), and a refrigerator (6) for providing a low-temperature environment is also arranged in the device body (1);
a stirring mechanism is arranged in the reaction cavity (3) to stir the inside of the reaction container (4), a filter cavity (7) is arranged in the device body (1), the filter cavity (7) is communicated with the reaction cavity (3) through a filter funnel (8), and a collecting container (9) is arranged below the filter funnel (8);
and a vacuum mechanism is also included for providing a vacuum environment for the reaction chamber (3) and the filter chamber (7).
2. The integrated device for efficient extraction, separation and preparation of collagen aggregates according to claim 1, wherein the integrated device is characterized in that: the top of the device body (1) is provided with a bracket (10) for fixing the reaction container (4), and the reaction bracket (10) is connected with the centrifugal machine (5).
3. The integrated device for efficient extraction, separation and preparation of collagen aggregates according to claim 1, wherein the integrated device is characterized in that: the vacuum mechanism comprises a vacuum pump (11) arranged on one side of the device body (1), the vacuum pump (11) is communicated with the inside of the reaction cavity (3) through a first pipeline (12), the vacuum pump (11) is communicated with the inside of the filter cavity (7) through a second pipeline (13), and valves (14) are arranged on the first pipeline (12) and the second pipeline (13).
4. The integrated device for efficient extraction, separation and preparation of collagen aggregates according to claim 1, wherein the integrated device is characterized in that: the stirring mechanism comprises an L-shaped supporting rod (15), one end of the L-shaped supporting rod (15) is fixedly connected with the device body (1), the other end of the L-shaped supporting rod (15) is arranged above the reaction container (4), one end of the L-shaped supporting rod (15) above the reaction container (4) is fixedly connected with a motor (16), the output end of the motor (16) is fixedly connected with a stirring rod (17), and the stirring rod (17) is arranged inside the reaction container (4).
5. The integrated device for efficient extraction, separation and preparation of collagen aggregates according to claim 1, wherein the integrated device is characterized in that: one side of the sealing cover (2) is provided with a first window (18) for opening the reaction cavity (3), and the first window (18) is arranged in a sealing way with the sealing cover (2).
6. The integrated device for efficient extraction, separation and preparation of collagen aggregates according to claim 1, wherein the integrated device is characterized in that: one side of the device body (1) is provided with a second window (19) for opening the filter cavity (7), and the second window (19) is arranged in a sealing way with the device body (1).
7. The integrated device for efficient extraction, separation and preparation of collagen aggregates according to claim 3, wherein: the valve (14) is an electromagnetic valve.
8. The integrated device for efficient extraction, separation and preparation of collagen aggregates according to claim 3, wherein: one side of the device body (1) is provided with a control mechanism, and the vacuum pump (11), the electromagnetic valve, the centrifugal machine (5), the refrigerator (6) and the motor (16) are all electrically connected with the control mechanism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321684121.3U CN220283954U (en) | 2023-06-29 | 2023-06-29 | Integrated device for efficient extraction, separation and preparation of collagen aggregate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321684121.3U CN220283954U (en) | 2023-06-29 | 2023-06-29 | Integrated device for efficient extraction, separation and preparation of collagen aggregate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220283954U true CN220283954U (en) | 2024-01-02 |
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ID=89334578
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321684121.3U Active CN220283954U (en) | 2023-06-29 | 2023-06-29 | Integrated device for efficient extraction, separation and preparation of collagen aggregate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220283954U (en) |
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2023
- 2023-06-29 CN CN202321684121.3U patent/CN220283954U/en active Active
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