RU102618U1 - INSTALLATION FOR COMBINED CULTIVATION OF MICROORGANISMS - Google Patents

Abstract

 1. Installation for the combined cultivation of microorganisms, including three horizontally located fermentation tanks with side covers, equipped with nozzles, respectively, for supplying aerating gas and venting the gas medium, nozzles for supplying and venting the liquid phase and matrices for solid substrate located in the internal cavities of the tanks, a buffer a container connected by pipelines with controlled valves, respectively, with channels for draining and collecting liquid product, a device for switching hydraulic pots connected by pipelines with controlled valves with a buffer tank, with external devices for supplying liquid nutrient media and inoculum, and with nozzles of fermentation tanks for supplying and discharging a liquid phase, sterilization filters in the channels for supplying aerated gas to fermentation tanks, sterilization filters and droplets in the channels of removal gases from the fermentation tanks, the liquid return outputs of the droplet separators by pipelines with controlled valves are communicated with the cavity of the corresponding fermentation tanks, and a control device, characterized in that it is equipped with containers for condensed nutrient media and for collecting fermentation products, which are connected via pipelines with valves to each other, with fermentation tanks and with a buffer tank, and each of the fermentation tanks is equipped with a collector for the formation of liquid flows components of the culture medium inside the fermentation tanks connected to the pipe for supplying and discharging the liquid phase, while the container for the condensed nutrient medium is provided with heat

Description

The utility model relates to biotechnology, and more specifically to equipment for the cultivation of microorganisms using liquid and solid substrates and microorganisms sensitive to mechanical stress, and can be used in the microbiological, medical, food industry and research practice.

When using substrates from renewable plant materials with a high content of insoluble components of lignocellulose, fiber, and starch in microbiological technologies, problems arise in ensuring mass and gas exchange in viscous culture media with a high content of insoluble components and automation of processes that implement these technologies, including the stages of loading the substrates and unloading products cultivation.

A known apparatus for the cultivation of cells and microorganisms (RU No. 69518, C1, publ. 12/27/2007) comprising two fermentation tanks with a lid and nozzles, respectively, for supplying aerating gas and venting the gas medium. Both tanks are equipped with matrices freely placed in the cavity of each tank, communicated with each other by a bottom drain pipe and connected by pipelines to controlled valves. The apparatus includes a pump for introducing into the first fermentation tank nutrient solutions and inoclum inoculum, a buffer tank connected by a pipeline to a pipe for venting the gas medium and pipelines with built-in controlled valves with fermentation tanks and with a lock chamber. The lock chamber is connected by pipelines with built-in controlled valves with a second fermentation tank and with pipes for draining and collecting the resulting products. Matrices can be in the form of a coiled metal mesh, into the inter-turn space of which a solid substrate is placed.

The apparatus does not provide a continuous fermentation process, since in order to extract spent solid substrate and insoluble fermentation products, in particular microbial biomass, you need to stop the process, open both fermentation tanks, remove the matrices and unload the product. After the process of unloading and cleaning, the fermentation modules are again collected and the sterilization process of the entire set of equipment and solid substrate is carried out. These procedures significantly reduce the productivity of the process and complicate the operation of the apparatus with large volumes of processing of solid substrates. The disadvantages also include the need to grind solid insoluble components of the substrate to increase their reactivity, while the grid matrix becomes ineffective for fixing the crushed substrate, which is necessary when cultivating mycelial forms of microorganisms.

A known apparatus for the combined cultivation of microorganisms, selected for the prototype (RU No. 93394 publ. 04/27/2010). The apparatus contains three horizontally located fermentation tanks with side covers and matrices for a solid substrate, placed inside the tanks, pipes for the liquid phase of the culture medium, pipes and pipes with valves in the supply channels to the upper cavity of the fermentation tanks of the aeration gas and exhaust gases from the fermentation tanks, buffer tank, hydraulic flow switching device, droplet separators, bacterial filters and control device.

The disadvantages of the prototype include the need for manual loading of the substrate and unloading of the fixed biomass of microorganisms and insoluble fermentation products from matrices.

In the fermentation process, the biomass of microorganisms builds up on the multiple surfaces of the matrix in a dense layer. With a layer thickness of more than 4 mm, microorganisms located under the biomass layer on the matrix surfaces do not receive proper nutrition and die with the formation of toxic biomass decomposition products, which reduces the quality of the resulting products.

To maintain the quality of the products obtained, one of the fermentation tanks, which is freed from the culture fluid, is removed from the fermentation process. In the remaining containers, the process continues under solid-state fermentation, and the following manual procedures are carried out with the tank removed from the fermentation process:

- removal of the matrix from the fermentation tank;

- removal of biomass of microorganisms from numerous matrix surfaces;

- loading the matrix with a solid substrate and placing it in a fermentation tank;

- sterilization of the container and its return to the fermentation process.

The implementation of these procedures requires the use of manual labor, a significant expenditure of consumables, energy and time, and leads to a decrease in the efficiency of the process and an increase in the cost of manufactured products.

The disadvantages include local input into the fermentation tanks of the liquid phase of the culture medium, which leads to its uneven distribution when the working fluid is mixed in the fermentation tanks, especially in the cavities farthest from the specified input. Inadequate mixing of the working fluid promotes the formation of stagnant zones and the deposition of biomass of microorganisms on the walls of fermentation tanks, which reduces the quality of process control and, as a result, reduces the quality of fermentation products.

It is known that the food and agricultural sectors of the economy have industrial waste, which is a valuable raw material for biotechnology. Liquid wastes of these industries are thickened for easy storage and converted into feed additives by fermentation. To obtain complete nutrient media used in biotechnology, these wastes are enriched with various balanced additives, for the introduction of which it is preferable to have additional aseptic inputs in the plants. In the known apparatus there is no additional sterile input of the medium, which reduces the possibility of using the apparatus.

The technical result consists in increasing the efficiency of the production process and reducing the cost of manufactured products by automating the loading and collection of spent biomass, as well as expanding the scope of the installation for combined cultivation of microorganisms in the fermentation processes of different types of microorganisms, fungi and cells.

The technical result is achieved by the fact that the installation for the combined cultivation of microorganisms includes three horizontally located fermentation tanks with side covers, equipped with nozzles for supplying aerating gas and venting the gas medium, nozzles for supplying and venting the liquid phase and matrices for the solid substrate, located in the inner tank cavities, a buffer tank connected by pipelines to controlled valves, respectively, with channels for draining and collecting liquid product, hydraulic flow switching device, connected by pipelines with controlled valves with buffer capacity, with external supply devices for liquid nutrient media and inoculum, and with nozzles of fermentation tanks for supplying and discharging a liquid phase, sterilization filters in the channels for supplying aerated gas to fermentation tanks, sterilization filters and droplet separators in the channels for the removal of gases from fermentation tanks, the liquid return outputs of the droplet separators are connected to the floor by pipelines with controlled valves the corresponding fermentation tank and the control device is additionally equipped with tanks for condensed nutrient media and for collecting fermentation products, which are connected through pipelines with valves to each other, with fermentation tanks and with a buffer tank, and each of the fermentation tanks is equipped with a collector for the formation of flows of liquid components the culture medium inside the fermentation tanks connected to the pipe for supplying and discharging the liquid phase, while the container for condensed feed the medium is equipped with a heat exchange jacket, a loading pipe with a sealed plug, a valve for introducing compressed air into the tank and a discharge pipe, and a tank for collecting fermentation products is equipped with valves for entering and removing compressed air and pipelines with valves for the flow of liquid components of the culture medium between the tank for food collection and fermentation tanks;

and also the fact that the matrices for the solid substrate are made in the form of movable nozzle matrices in the form of Raschig rings;

as well as the fact that the collectors are mounted on the lids of the containers with the possibility of rotation and provided with holes.

The use of a container for collecting fermentation products allows us to automate the collection of solid insoluble fermentation products and, as a result, significantly reduce the time and production costs of carrying out it, moreover, biomass removal is carried out by mechanically movable nozzle matrices without the use of additional dilution and solvents that complicate the isolation of the final products cultivation of microorganisms.

The use of collectors for the formation of flows and elastic streams of culture fluid, washing and mixing matrix nozzles in fermentation tanks, improves the quality of control of fermentation processes due to uniform mixing and a significant reduction in the zones of localization of biomass of microorganisms on the inner surface of fermentation tanks. The installation position of the collectors allows the formation of fluid flows in fermentation tanks with different hydrodynamic effects on cultured microorganisms, which expands the scope of application of different types of microorganisms, fungi and cells in fermentation processes.

The use of a container for a thickened nutrient medium and equipping the lock chamber with additional valves for loading and unloading a fixed portion of this nutrient medium allows us to automate the process of preparing a complete nutrient medium in a fermentation process, which significantly expands the scope of the installation.

A utility model is illustrated by a drawing, which illustrates but does not limit it.

Figure 1, presents a diagram of the installation for the combined cultivation of microorganisms.

The installation contains three horizontally located fermentation tanks 1, 2, 3 with side covers 4, 5, 6, for loading and unloading matrix nozzles and with covers 7, 8, 9, on which are located the nozzles (not shown) of the aeration inlet gas into the upper cavity of each of the containers. The nozzles are respectively connected to the control valves 10, 11, 12, supply aerating gas in the tank, incoming through the inlet gas filters 13, 14, 15, ensuring the sterility of the gas stream. The pipes for the removal of metabolite gases from the upper cavity of the fermentation tanks 1, 2, 3 are connected through controlled valves 16, 17, 18, the corresponding droplet separators 19, 20, 21 and filters 22, 23, 24, ensuring the sterility of the exhaust gases, and valves 25, 26, 27 with the pipeline "off-gas".

Inside the fermentation tanks 1, 2, 3 are placed matrix nozzles 28, 29, 30, for example, in the form of Raschig rings.

Fermentation tanks 1, 2, 3 to provide the required temperature conditions are equipped with thermostatic shirts 31, 32, 33, into which a coolant of a given temperature is supplied.

The supply of sterile culture media and inoculum to the fermentation tanks 1, 2, 3 is carried out through controlled valves 34, 35, 36, hydraulic fluid switching device 37 and controlled valves 38 or 39, respectively. Dosed sampling of liquid cultivation products from fermentation tanks is carried out through a hydraulic flow switching device 37, a controlled valve 41 into a buffer tank 40, which is connected through a controlled valve 43 to an external sample receiver (not shown in Fig. 1) and through a controlled valve 42 to a source aerating air.

For sterilization of the buffer tank 40 by flowing steam, it is connected to pipelines with a controlled valve 44 with a steam source and a controlled valve 45 with the "Drain" pipeline.

To discharge the liquid accumulated in the droplet separators 19, 20, 21, they are connected through controlled valves 46, 47 and 48 to the fermentation tanks 1, 2, 3, respectively.

Filters 13, 14, 15 are connected by pipelines with controlled valves 49, 50, 51 with a drain line to remove the accumulated liquid in them and pipelines with controlled valves 52, 53, 54 with a steam source (not shown in Fig.).

Through the controlled valves 55, 56, 57, the flow of steam is supplied to the filters 22, 23, 24.

For autonomous sterilization of each of the fermentation tanks 1, 2 and 3, its lower discharge pipes are connected through controlled valves 58, 59, 60, respectively, to the “Drain” channel.

The inputs of the filters 13, 14, 15 for supplying aeration air are connected through the corresponding controlled valves 61, 62, 63 with the channel "Aeration".

The operation of the installation for combined (liquid-phase and solid-phase) cultivation of microorganisms is controlled by a control device 64.

In order to eliminate stagnant zones and ensure uniform filling and emptying of the liquid phase of the culture medium throughout the fermentation tanks with the formation of dripping films on the nozzle matrices, the unit is equipped with collectors 65, 66 and 67 with openings 68. The collectors are fixed to the covers 7, 8 and 9 of fermentation tanks 1, 2 and 3.

For storage of the initial substrates in the form of condensed matter of the nutrient medium, the installation is equipped with a capacity of 69 with a heat exchange jacket 70, a loading pipe 71 with a sealing plug 72 and controlled valves 73 for introducing compressed air “Charge” into the cavity of the tank 69 and 74 for introducing a solution of condensed components nutrient medium in the lock chamber 40.

To collect biomass and other fermentation products, a container 75 with a network of pipelines 76 and controlled valves 77-84 is provided.

Capacity 75 for collecting fermentation products, is equipped with a valve 77 for introducing compressed air "Charge" and a valve 78 for discharging compressed air into the channel "exhaust gases". Pipelines 76 with valves 79-83 provide a flow of culture fluid between tank 75 and tanks 1, 2 and 3, as well as the culture medium is supplied to the lock chamber 40 when dosed. Complete discharge of the product from the tank 75 is carried out through the valve 84 through the channel "Product".

The collectors 65-67 for the formation of culture fluid streams are provided with holes 68 made in the plane of the axial section of each collector and placed in the lower part of the cavity of each of the fermentation tanks 1-3. The collectors 65-67 are mounted on the end caps of 7-9 tanks 1-3 and have three mounting positions: holes up, holes sideways and holes down. The collectors 65-67 through controlled valves are connected to the pipeline network for the flow of culture fluid through the device switching hydraulic flows 37.

A solid plant substrate containing insoluble components of lignocellulose, starch, fiber is placed on the nozzle matrices. Before loading onto the matrix nozzles 28, 29, 30, the substrate is prepared for use based on the specific characteristics of the substrate itself, for example, it is crushed, cleaned of inhibitory components, its reactivity is increased by various methods, and also taking into account the peculiarities of the realized fermentation processes.

Installation works as follows.

Before starting work, the plug 72 is removed from the pipe 71 and the solution of condensed substances, including insoluble components of the nutrient medium, is loaded into the container 69, after which the pipe 71 is sealed with a plug 72, and a heat transfer medium with a predetermined sterilization or pasteurization temperature of the condensed substances is passed through the heat exchange jacket 70. After the heating time for the condensed substances has expired, a valve 73 is opened, through which sterile air of a given pressure will begin to flow into the container 69.

The nutrient medium is prepared in fermentation tanks 1, 2 and 3 automatically at the stage of the technological process of preparation of the nutrient medium - "Nutrient medium". The implementation of the stage is carried out by the control device 64, on which the number of cycles of the airlock 40 is set.

Stage "Culture medium".

The stage of preparation of the nutrient medium is carried out with the consistent implementation of the following procedures.

The procedure "Condensed fraction." The procedure provides a batch loading of the solution of condensed matter from the tank 69 into the fermentation tank 3 through the operation of the lock chamber 40. Valves 44 and 45 open and the water vapor heated to the set temperature passes through the valve 44 to the chamber 40 and is discharged through the valve 45 to the drain manifold in the form of a vapor-air mixture. Then the valves 44 and 45 are closed. The air free chamber 40 is cooled by ambient air.

Valve 74 - opens and the thickened components of the nutrient medium, which are under air pressure in the tank 69 are displaced into the chamber 40 until they are completely filled, after which the valve 74 - closes. Further valves 44; 83 and 18 open.

Water vapor enters the chamber 40, the pressure of which a portion of the condensed medium from the chamber 40, the valve 83 and the collector 67 are forced into the tank 3. The air in the fermentation tank 3 through the valve 18, the droplet separator 21, the filter 24 and the initially open valve 27 is discharged into atmosphere, then valves 83; 44 and 18 are closing.

One cycle of chamber 40 has been completed. Chamber 40 is free of air and prepared for loading another portion of the condensed medium. The procedure "Condensed fraction" ends after working out a given number of cycles of operation of the camera 40.

Then the procedure is carried out "Liquid fraction". It provides the loading of a solution of corrective substances in the tank 3 for the preparation of a complete nutrient medium. The valves 38; 36 and 18 open. From an external source, for example, from a solution sterilizer, through a valve 38, a hydraulic flow switching device 37, a valve 36, and a manifold 67, a solution of correction substances in a predetermined volume will be supplied to a container 3, and air in a container 3 will be vented to the atmosphere through a valve 18, a droplet separator 21, a bacterial filter 24 and an initially open valve 27. The procedure ends when a substance solution in a predetermined volume and valves 38 are introduced into the container 3; 36 and 18 close.

Then the procedure is "Stirring". It is carried out for mixing a solution of condensed matter with a solution of corrective substances and wetting the resulting medium of the multiple surfaces of nozzles 28-30 placed in fermentation tanks 1-3. In this case, the valves 12; 36; 34 and 16 open.

In the tank 3, through the initially open valve 63, the filter 15 and the valve 12 receives aeration air. Under air pressure, the medium in tank 3 passes through a manifold 67, a valve 36, a hydraulic flow switching device 37, a valve 34 and a manifold 65 flows into a tank 1, and air from the tank 1 is vented to the atmosphere through a valve 16, a droplet separator 19, a filter 22 and an initially open valve 25. Then, the valves 12 and 16 are closed and the valves 10 and 18 open.

In the tank 1, through the initially open valve 61, the filter 13 and the valve 10 receives aeration air. Under air pressure, the medium is located in tank 1, through valve manifold 65, valve 34, hydraulic flow switching device 37, valve 36 and manifold 67, flows into tank 3, and air from tank 3 is vented to atmosphere through valve 18, droplet separator 21, filter 24 and initially open valve 27. Then the valves 10; 18 and 34, the valves 12 are closed and opened; 35 and 17. In the tank 3, through the initially open valve 63, the filter 15 and the valve 12 receives aeration air. Under air pressure, the medium in tank 3 passes through a manifold 67, valve 36, hydraulic flow switching device 37, and valve 35 into the tank 2, and air from the tank 2 is vented to the atmosphere through a valve 17, a droplet separator 20, a filter 23 and the original open valve 26. After that, valves 17 and 12 are closed and valves 11 and 18 are opened. At the same time, aerating air enters the tank 2 through the initially open valve 62, filter 14 and valve 11. Under air pressure, the medium in tank 2 passes through a manifold 66, valve 35, a hydraulic flow switching device 37, and valve 36 into the tank 3, and air from the tank 3 is vented to the atmosphere through a valve 18, a droplet separator 21, a filter 24 and the original open valve 27. The “Stirring” procedure is completed and valves 11; 35; 36 and 18 close.

Next, the procedure "Sowing". It provides the introduction into the prepared nutrient medium of microorganisms - producers of target products with the formation of a culture fluid. The procedure is as follows, valves 39; 36 and 18 are also opened from the inoculum source, through valve 39, hydraulic flow switching device 37, valve 36 and manifold 67; a predetermined volume of microorganisms is introduced into container 3, then valves 39; 36 and 18 close.

The process of cultivating microorganisms begins.

Before the process of cultivation of microorganisms on the device 64, ask:

- the temperature in the heat-exchange shirts 31-33;

- aeration air flow rate;

- the flow time of the culture fluid from one fermentation tank to another;

- fermentation time.

The process of cultivation of microorganisms is carried out with the continuous implementation of the following procedures.

Valves 12; 36; 35 and 17 - open and in the tank 3, through the initially open valve 63, filter 15 and valve 12 receives aerating air. The culture fluid from tank 3, through aerating air pressure, through manifold 67, valve 36, hydraulic flow switching device 37, valve 35 and manifold 66 flows into tank 2, and air from tank 2 is vented to the atmosphere through valve 17, droplet separator 20, filter 23 and the initially open valve 26. The culture fluid located in the tank 3, flows from the surfaces of the nozzle matrix 30 with the formation of multiple films in contact with aerating air, which allows you to activate the respiratory activity of microorganisms and optimize fermentation conditions. Matrix nozzles 29 located in the tank 2, washed by the culture fluid coming from the tank 3, which activates the mixing of the culture fluid and promotes the formation of new films of the culture fluid on the surfaces of the nozzles 29.

Then the valves 12; 36 and 17 close and open the valves 34; 16 and 11. In the tank 2, through the initially open valve 62, the filter 14 and the valve 11 receives aeration air. The culture fluid from tank 2, through aerating air pressure, through manifold 66, valve 35, hydraulic flow switching device 37, valve 34 and manifold 65 flows into tank 1, and air from tank 1 is vented to atmosphere through valve 16, droplet separator 19, filter 22 and the initially open valve 25. The culture fluid located in the tank 2 flows from the surfaces of the nozzle matrix 29 with the formation of multiple films in contact with aerating air, which allows you to activate the respiratory activity of microorganisms and optimize fermentation conditions.

Matrix nozzles 28 located in the tank 1 are washed by the culture fluid coming from the tank 2, which activates the mixing of the culture fluid and promotes the formation of new films of the culture fluid on the surfaces of the matrix nozzles 28.

Then the valves 16; 35 and 11, the valves 10 are closed and opened; 36 and 18. In the tank 1, through the initially open valve 61, the filter 13 and the valve 10 receives aerating air. The culture fluid from the tank 1, through the pressure of the aeration air, through the manifold 65, the valve 34, the hydraulic flow switching device 37, the valve 36 and the manifold 67 flows into the tank 3, and the air from the tank 3 is discharged into the atmosphere through the valve 18, the droplet separator 21, the filter 24 and the initially open valve 27. The culture fluid located in the tank 1 drains from the surfaces of the nozzles 28 with the formation of multiple films in contact with aerating air, which allows to activate the respiratory activity of microorganisms and optimizes Obtain fermentation conditions. Matrix nozzles 30, located in the tank 3, washed by the culture fluid coming from the tank 1, which activates the mixing of the culture fluid and promotes the formation of new films of the culture fluid on the surfaces of the matrix nozzles 30. Then the valves 10; 36; 18 and 34 close.

The indicated sequences of actions during the cultivation of microorganisms are repeated continuously until a predetermined time elapses during which the growth of microorganisms takes place, moreover, when the fermentation containers are filled with liquid components of the culture medium, a liquid-phase process is carried out, and in empty containers the process is carried out in the mode of solid-phase film cultivation. The process of cultivating microorganisms in the fermentation tank 3 ends with the procedure of filling this tank with the liquid components of the culture medium, while the valves 12, 18, 36 and 48 are closed and the tank 3 is prepared for the procedure of unloading biomass and other fermentation products into the tank 75.

At the same time, fresh nutrient medium is prepared by repeating the "Nutrient medium" stage. Fresh nutrient medium is supplied to the fermentation tank 2 when the valve 80 is opened, then the process of cultivating microorganisms begins when repeating the transfer procedures from the tank 2 to the tank 1 and from the tank 1 to the tank 2 through the hydraulic flow switching device 37, as described above. With a decrease in producer activity, the “Sowing" procedure is additionally performed, and the inoculum, when the valve 39 is opened, is sent to the fermentation tanks 1 and 2.

Simultaneously with the above processes, the following procedures are performed:

- "drain" of fermentation products from the tank 3 into the tank 75;

- cleaning matrix nozzles from the biomass of microorganisms in the tank 3.

When carrying out the procedure "drain" valves 12; 79 and 78 open and into the tank 3 through the initially open valve 63, the filter 15 and the valve 12 receives aeration air. The culture fluid from the tank 3, under the pressure of aerating air, flows through the collector 67 and open valves 81 and 84 into the tank 75, and the air from the tank 75 is vented to the atmosphere through the valve 78. A layer of grown biomass of microorganisms remains on the surfaces of the nozzles 30, which must be unloaded into capacity 75.

For this purpose, the procedure "Cleaning nozzles." In this case, the valves 78 and 12 are closed and the valves 77 and 18 open.

Compressed air is introduced into the container 75, through the valve 77, the pressure of which the culture fluid, through the pipe 76, through the valve 81, is pumped into the manifold 67 and in the form of elastic jets formed by the openings 68 and intensively affects the matrix nozzles 30. Air from the tank 3 is vented to the atmosphere through a valve 18, a droplet separator 21, a filter 24 and an initially open valve 27. Under the influence of the jets of the culture fluid, nozzles 30 in the tank 3 are mixed and freed from the deposited biomass of microorganisms. Then, valves 77 and 18 are closed and valves 78 and 12 open.

In the tank 3, through the initially open valve 63, the filter 15 and the valve 12 receives aeration air. The culture fluid and biomass of microorganisms, washed from the surfaces of the nozzle matrix 30, from the tank 3 through the collector 67 and the valve 81 flows into the tank 75, and the air from the tank 75 is vented to the atmosphere through the valve 78. The cleaning procedure of the matrix nozzles is repeated a predetermined number of times, then valves 78 and 12 close.

Further, the valves 77 and 82 can be opened by the signals of the control device 64 and the biomass of microorganisms and other fermentation products from the tank 75 through the valve 82 are discharged through the Product channel.

After unloading the tank 75, the valves 82 and 77 are closed, and the tank 77 is prepared for unloading another fermentation tank, for example, a fermentation tank 2, if it has completed the process of growth of microorganisms and obtaining the final product. The repetition of the stages and procedures continues until the complete cultivation of microorganisms.

Claims (3)

1. Installation for the combined cultivation of microorganisms, including three horizontally located fermentation tanks with side covers, equipped with nozzles, respectively, for supplying aerating gas and venting the gas medium, nozzles for supplying and venting the liquid phase and matrices for solid substrate located in the internal cavities of the tanks, a buffer a container connected by pipelines with controlled valves, respectively, with channels for draining and collecting liquid product, a device for switching hydraulic pots connected by pipelines with controlled valves with a buffer tank, with external devices for supplying liquid nutrient media and inoculum, and with nozzles of fermentation tanks for supplying and discharging a liquid phase, sterilization filters in the channels for supplying aerated gas to fermentation tanks, sterilization filters and droplets in the channels of removal gases from the fermentation tanks, the liquid return outputs of the droplet separators by pipelines with controlled valves are communicated with the cavity of the corresponding fermentation tanks, and a control device, characterized in that it is equipped with containers for condensed nutrient media and for collecting fermentation products that are connected via pipelines with valves to each other, with fermentation tanks and with a buffer tank, and each of the fermentation tanks is equipped with a collector for the formation of liquid flows components of the culture medium inside the fermentation tanks connected to the pipe for supplying and discharging the liquid phase, while the container for the thickened nutrient medium is provided with heat a jacket, a loading nozzle with a sealed plug, a valve for introducing compressed air into the tank and an unloading nozzle, and the tank for collecting fermentation products is equipped with valves for entering and removing compressed air and pipelines with valves for the flow of liquid components of the culture medium between the tank for collecting products and fermentation tanks. 2. The apparatus according to claim 1, characterized in that the matrices for the solid substrate are made in the form of movable nozzle matrices in the form of Raschig rings. 3. The apparatus according to claim 1, characterized in that the collectors are mounted on the lids of the containers with the possibility of rotation and provided with holes.