CN220166173U - Operating bottle for soil microorganism culture and fumigation - Google Patents
Operating bottle for soil microorganism culture and fumigation Download PDFInfo
- Publication number
- CN220166173U CN220166173U CN202321111221.7U CN202321111221U CN220166173U CN 220166173 U CN220166173 U CN 220166173U CN 202321111221 U CN202321111221 U CN 202321111221U CN 220166173 U CN220166173 U CN 220166173U
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- CN
- China
- Prior art keywords
- bottle
- soil
- fumigation
- cap
- opening
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000002689 soil Substances 0.000 title claims abstract description 49
- 244000005700 microbiome Species 0.000 title claims abstract description 43
- 238000003958 fumigation Methods 0.000 title claims abstract description 21
- 238000009423 ventilation Methods 0.000 claims abstract description 16
- 239000012528 membrane Substances 0.000 claims description 21
- 239000002985 plastic film Substances 0.000 claims description 5
- 229920006255 plastic film Polymers 0.000 claims description 5
- 239000004033 plastic Substances 0.000 claims description 3
- 238000007731 hot pressing Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 9
- 238000002474 experimental method Methods 0.000 abstract description 7
- 238000011282 treatment Methods 0.000 abstract description 6
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 238000012545 processing Methods 0.000 abstract description 3
- 238000005303 weighing Methods 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 abstract 1
- 238000007789 sealing Methods 0.000 description 5
- 230000000813 microbial effect Effects 0.000 description 3
- 239000004743 Polypropylene Substances 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to an operation bottle for cultivating and fumigating soil microorganisms, which comprises a bottle body, a bottle cap and a breathable film, wherein the top of the bottle body is provided with a bottle opening; the bottle cap is connected to the bottle mouth, and the top of the bottle cap is provided with an opening; the ventilation film is arranged at the opening and is connected with the bottle cap; the device is convenient for accommodating soil and gas exchange, and can be used as a special tool for culturing and fumigating soil microorganisms; the operation can be simplified, the manpower and time are saved, the efficiency is improved, a convenient operation basis is provided for the pretreatment processes of soil microorganism cultivation, fumigation and the like by the testers, and a large number of sample treatments are facilitated; the soil weighing machine has the advantages of reasonable structure, easiness in processing and manufacturing, cost reduction, convenience in carrying and operation, convenience in weighing soil and improvement of operation safety; the temperature and the humidity are convenient to control in the culture experiment process; the gas exchange is convenient, the gas throughput is greatly improved in fumigation experiments, so that the chloroform can kill microorganisms with high efficiency, and a good experimental effect is achieved.
Description
Technical Field
The utility model relates to an operating bottle for cultivating and fumigating soil microorganisms.
Background
At present, the soil needs to be subjected to microbial cultivation and fumigation treatment before microbial biomass measurement is performed on the soil.
In the prior art, when carrying out microbial cultivation and fumigation treatment to soil, adopt the wide-mouth bottle to hold soil more to generally fix ventilative sealing membrane in wide-mouth bottle top bottle mouth department through the rubber band, but have following problem easily in the use: 1. as the wide-mouth bottle belongs to a universal experimental device, no special tool for cultivating and fumigating soil microorganisms exists; 2. when the wide-mouth bottle is adopted, the sealing film is required to be fastened and fixed at the opening of the wide-mouth bottle through the rubber band, so that the operation process is complicated; especially when a large number of sample treatments are performed, a large amount of manpower and time are consumed.
Disclosure of Invention
In view of at least one of the above problems in the prior art, an object of the present utility model is to provide an operating bottle for cultivation and fumigation of soil microorganisms, which is suitable as a special tool for cultivation and fumigation of soil microorganisms; the sealing film is fastened and fixed at the opening of the wide-mouth bottle without manual operation through rubber bands, so that the operation is simplified; the method is more suitable for processing a large amount of soil samples, and saves manpower and time.
In order to achieve the above purpose, the present utility model adopts the following technical scheme:
a handling bottle for soil microorganism culture and fumigation comprising:
the top of the bottle body is provided with a bottle opening;
the bottle cap is connected to the bottle opening, and an opening is formed in the top of the bottle cap;
and the breathable film is arranged at the opening and is connected with the bottle cap.
Preferably, the breathable film comprises at least three layers of film body.
Preferably, the membrane body positioned in the middle layer is provided with a sterile filter membrane, and the membrane bodies on the upper layer and the lower layer are both provided with plastic membranes.
Preferably, the plastic film is provided with ventilation holes.
Preferably, the number of the ventilation holes is at least two, and the ventilation holes are uniformly distributed.
Preferably, the opening is a round hole and is arranged in the center of the top surface of the bottle cap.
Preferably, the breathable film is attached to the bottle cap by heat pressing.
Preferably, the bottle cap is cylindrical, and the bottle cap is sleeved and connected on the bottle opening.
Preferably, the bottle mouth is provided as a circular tube, and the bottle cap is provided as a circular cylinder.
Preferably, an inner thread is arranged on the inner wall of the bottle cap, an outer thread is arranged on the outer wall of the bottle opening, and the bottle cap is in threaded connection with the bottle opening.
Due to the adoption of the technical scheme, the utility model has the following advantages:
1. the operating bottle for cultivating and fumigating the soil microorganisms is convenient for accommodating the soil and exchanging gas, and can be used as a special tool for cultivating and fumigating the soil microorganisms; the device can simplify operation, save manpower and time, improve efficiency, provide more convenient operation equipment foundation for the pretreatment processes of soil microorganism culture, fumigation and the like by testers, and facilitate a large number of sample treatments.
2. The operating bottle for cultivating and fumigating soil microorganisms has reasonable structure, is easy to process and manufacture, can reduce cost, is convenient to carry and operate, and is convenient for weighing soil.
3. The operating bottle for cultivating and fumigating soil microorganisms is convenient for controlling temperature and humidity in the cultivation experiment process; the gas exchange is convenient, the gas throughput is greatly improved in fumigation experiments, so that the chloroform can kill microorganisms with high efficiency, and a good experimental effect is achieved.
4. According to the operating bottle for cultivating and fumigating soil microorganisms, the breathable film can play a role in filtering through the breathable sterile filter film, so that gas can only pass freely, and microorganisms and the like can be effectively prevented from entering and exiting through the film, so that the operating bottle is suitable for cultivating and fumigating soil microorganisms, gas exchange is safer and more effective, and experimental results are more accurate.
5. According to the operating bottle for cultivating and fumigating soil microorganisms, the bottle cap and the bottle mouth can be connected through threads, so that the connecting efficiency and the fastening effect of the bottle cap and the bottle mouth are improved; the widths of the external threads and the internal threads which are matched with each other can be properly increased, so that the time for connecting the bottle cap threads on the bottle mouth is saved, and the efficiency of screwing the bottle cap on the bottle mouth through the threads is improved.
Drawings
Fig. 1 is a schematic structural view of a manipulation bottle for soil microorganism cultivation and fumigation according to an embodiment of the present utility model.
Fig. 2 is a bottom view of a bottle cap of a manipulation bottle for soil microorganism cultivation and fumigation, according to an embodiment of the present utility model.
The reference numerals in the drawings:
1 is a bottle body, 2 is a bottle cap, 3 is a ventilated membrane, 301 is a ventilation hole.
Detailed Description
In order to make the objects, technical solutions and advantages of the present utility model more apparent, the technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings. It will be apparent that the described embodiments are some, but not all, embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
In the description of the present utility model, it should be noted that the azimuth or positional relationship indicated by the terms "upper", "lower", "front", "rear", etc. are based on the azimuth or positional relationship shown in the drawings, and are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the system or element referred to must have a specific azimuth, be configured and operated in a specific azimuth, and thus should not be construed as limiting the present utility model.
In the description of the present utility model, it should be noted that, unless explicitly stated and limited otherwise, the terms "mounted," "disposed," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.
According to the operating bottle for cultivating and fumigating soil microorganisms, the bottle cap is covered at the bottle opening at the top of the bottle body, the top of the bottle cap is provided with the opening, and the top of the bottle cap is provided with the ventilated membrane; the operating bottle is convenient for gas exchange, and can be used as a special tool for cultivating and fumigating soil microorganisms; in the using process, an experimenter is not required to fix the breathable sealing film at the bottle mouth through the rubber band, so that the operation is simplified, and the efficiency is improved; and when a large amount of samples are processed, the labor and time are saved.
Hereinafter, embodiments of the present utility model will be described in detail with reference to the accompanying drawings.
Examples
Referring to fig. 1 and 2 in combination, the present embodiment provides an operating bottle for cultivating and fumigating soil microorganisms, comprising:
the top of the bottle body 1 is provided with a bottle opening;
the bottle cap 2 is connected to the bottle mouth, and an opening is formed in the top of the bottle cap 2;
and the breathable film 3 is arranged at the opening and is connected with the bottle cap 2.
The operating bottle for cultivating and fumigating soil microorganisms in the embodiment is used for containing soil in a bottle body 1, the soil contains microorganisms, the operating bottle is used for cultivating and fumigating the soil microorganisms, and a bottle cap 2 is used for covering a bottle mouth at the top of the bottle body 1. The center of the bottle cap 2 is provided with an open design, the open design can be a round hole, and the ventilation film 3 is hot-pressed at the open edge of the center of the bottle cap 2. The breathable film 3 can play a role in ventilation; the operating bottle can be used as a special tool for cultivating and fumigating soil microorganisms, and an experimenter is not required to fix the breathable sealing film at the bottle mouth through rubber bands in the use process, so that the operation is simplified, and the efficiency is improved; when doing benefit to carrying out a large amount of sample processing, use manpower sparingly and time, avoid damaging operating personnel's hand, improve the safety in utilization.
In some embodiments, the bottle cap 2 is provided in a cylindrical shape, the top of which is provided with a top surface with a central opening, and the ventilation film 3 is provided on the top surface of the bottle cap 2; the bottle cap 2 is sleeved and connected on the bottle mouth.
For example, the bottle mouth is provided as a circular tube, and the bottle cap 2 is provided as a circular cylinder. The inner wall of the bottle cap 2 is provided with an internal thread, the outer wall of the bottle mouth is provided with an external thread, and the bottle cap 2 is in threaded connection with the bottle mouth.
The widths of the external threads and the internal threads which are matched can be properly increased, so that the time for connecting the bottle cap 2 on the bottle mouth in a threaded manner is saved, and the efficiency of screwing the bottle cap 2 on the bottle mouth through threads is improved.
Referring to fig. 2, in some embodiments, the gas-permeable membrane 3 is attached to the top surface of the bottle cap 2 from the inside of the bottle cap 2 by hot pressing, and is sealed at the opening.
In some embodiments, the breathable film 3 comprises at least three layers of film body. The multi-layer film body of the ventilated film 3 comprises a plastic film with ventilation holes on the top layer and the bottom layer, and a breathable sterile filter film on the middle layer.
For example, the air-permeable membrane 3 includes three membrane bodies, the membrane body of the middle layer is provided as a sterile filter membrane, and the membrane bodies of the upper and lower layers are both provided as plastic membranes. The three-layer film body structure is tightly compacted to form the breathable film 3 of the sterile breathable film structure.
Referring to fig. 1 and 2, the plastic film is provided with at least two ventilation holes 301, and the ventilation holes 301 are uniformly distributed on the ventilation film 3. The breathable film 3 may be provided in a circular shape. The ventilation holes 301 may also be provided as circular holes.
Wherein, the plastic film with the ventilation holes 301 forms a non-closed film body, which is convenient for gas exchange; the sterile filter membrane with air permeability can play a role in filtering, only enables gas to pass freely, effectively prevents microorganisms and the like from entering and exiting through the membrane, is suitable for culture and fumigation experiments of soil microorganisms, and enables the treatment operation before the experiments to be more convenient, the gas exchange to be safer and more effective, and the experimental results to be more accurate.
The bottle body 1 of the operating bottle for cultivating and fumigating soil microorganisms in the above embodiment may be a PP (polypropylene) bottle body, which has a light weight and is used for containing soil containing microorganisms in the microorganism cultivation and fumigating experiments. The breathable film 2 may be circular and may have a diameter of 2cm.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present utility model, and are not limiting; although the utility model has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present utility model.
Claims (8)
1. A manipulation bottle for soil microorganism cultivation and fumigation, comprising:
a bottle opening is arranged at the top of the bottle body (1);
the bottle cap (2) is connected to the bottle opening, and an opening is formed in the top of the bottle cap (2);
the breathable film (3) is arranged at the opening and is connected with the bottle cap (2);
the breathable film (3) comprises at least three layers of film bodies;
the membrane body positioned in the middle layer is provided with a sterile filter membrane, and the membrane bodies on the upper layer and the lower layer are both provided with plastic membranes.
2. The operating bottle for cultivation and fumigation of soil microorganisms according to claim 1, wherein said plastic film is provided with ventilation holes (301).
3. Operating bottle for the cultivation and fumigation of soil microorganisms according to claim 2, characterized in that said ventilation holes (301) are provided in at least two and uniformly distributed.
4. Operating bottle for the cultivation and fumigation of soil microorganisms according to claim 1, characterized in that said opening is a circular hole, arranged in the centre of the top surface of said cap (2).
5. Handling bottle for cultivation and fumigation of soil microorganisms according to claim 1, characterized in that said vapor permeable membrane (3) is attached to said bottle cap (2) by hot pressing.
6. Operating bottle for the cultivation and fumigation of soil microorganisms according to any one of claims 1 to 5, characterized in that said cap (2) is provided in a cylindrical shape, said cap (2) being connected over said mouth.
7. Operating bottle for the cultivation and fumigation of soil microorganisms according to claim 6, characterized in that said mouth is provided as a circular tube and said cap (2) is provided as a circular cylinder.
8. The operating bottle for cultivating and fumigating soil microorganisms according to claim 7, wherein the inner wall of the bottle cap (2) is provided with an inner thread, the outer wall of the bottle mouth is provided with an outer thread, and the bottle cap (2) is screwed on the bottle mouth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321111221.7U CN220166173U (en) | 2023-05-10 | 2023-05-10 | Operating bottle for soil microorganism culture and fumigation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321111221.7U CN220166173U (en) | 2023-05-10 | 2023-05-10 | Operating bottle for soil microorganism culture and fumigation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220166173U true CN220166173U (en) | 2023-12-12 |
Family
ID=89053375
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321111221.7U Active CN220166173U (en) | 2023-05-10 | 2023-05-10 | Operating bottle for soil microorganism culture and fumigation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220166173U (en) |
-
2023
- 2023-05-10 CN CN202321111221.7U patent/CN220166173U/en active Active
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| GR01 | Patent grant | ||
| GR01 | Patent grant |