CN220034529U - Porous shake box suitable for cell table glues board - Google Patents
Porous shake box suitable for cell table glues board Download PDFInfo
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- CN220034529U CN220034529U CN202321038792.2U CN202321038792U CN220034529U CN 220034529 U CN220034529 U CN 220034529U CN 202321038792 U CN202321038792 U CN 202321038792U CN 220034529 U CN220034529 U CN 220034529U
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- 239000003292 glue Substances 0.000 title claims description 4
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- 238000007789 sealing Methods 0.000 claims description 11
- 238000012258 culturing Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 230000003139 buffering effect Effects 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 2
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- 238000012216 screening Methods 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 abstract description 2
- 238000004114 suspension culture Methods 0.000 description 11
- 238000004113 cell culture Methods 0.000 description 8
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- 229920000515 polycarbonate Polymers 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of cell biology experiments, in particular to a porous shaking box suitable for a cell shaking table sticking plate, which comprises a shaking box shell, a buffer chamber and a culture chamber, wherein a plurality of arrays of deep holes are formed in the shaking box shell, the deep holes are used for placing lengthened gun heads, one side of a first section of each deep hole close to an opening is the buffer chamber, one side of a second section of each deep hole far away from the opening is the culture chamber, an arc ring is arranged between the first section and the second section, the arc ring limits the communication position of the first section and the second section of each deep hole to an arc opening, the arc opening is matched with the outer diameter of the lengthened gun heads, one side of each arc ring close to the opening of each deep hole is a straight surface, and one side of each arc ring far away from the opening of each deep hole is an arc inclined surface. Compared with the prior art, the utility model has the advantages that: can be firmly adhered to the shaking table adhesive plate; there are enough planes for writing marks; the probability of the culture splashing out of the box can be reduced, and pollution is avoided; specially designed for deep hole plate culture, and adapted in size; is suitable for high-flux cell strain screening culture or continuous subculture.
Description
Technical Field
The utility model relates to the technical field of cell biology experiments, in particular to a porous shaking box specially designed for cell suspension culture and suitable for cell shaking table sticky plates.
Background
Cell culture is a biotechnology which is accumulated by several generations of scientists and is vigorously developed so far, and the successful application of the cell culture in the field of biological pharmacy opens up a new era of producing medicines by cells for human beings. Cell culture is now a technique that can be used by every company or laboratory just as usual as in the traditional farming industry.
However, cell culture is highly dependent on modern culture equipment, unlike conventional culture, and once the culture conditions deviate or are wrong, the results are catastrophic, such as decreased cell viability, or even complete death. Thus, cell culture requires a complete set of specialized equipment and corresponding materials.
While the prior art has fully met the basic needs of cell culture, small volume high throughput cultures for specific needs have lacked specialized containers. In the past, suspension cells were required to be cultured in a static well plate, and since shake flask culture was not suitable when the number of cells was small, it was necessary to wait for the proliferation of cells to a certain amount before transferring into shake flask culture. Currently, many companies have begun to overcome this difficulty with deep well plates. Because the volume of each hole of the deep hole plate is small, the deep hole plate is suitable for small-volume suspension culture, and the holes are deep enough, and the culture solution cannot be splashed during shaking. Therefore, the use of the deep well plate can allow suspension culture with a small number of cells. The following reasons are why the related enterprises are eager for suspension culture:
1. suspension cells are far more suitable for large-scale production than adherent cells, such as antibody production. Suspension cells fill the culture volume with a greater yield per unit volume, while adherent cells occupy only the vessel walls.
2. The suspension cells can be cultured under the static condition, but under the condition of static culture and suspension culture, the growth speed, protein expression and other aspects of the cells are different, so that development progress and cell strain screening are affected.
3. Cells cultured in a static state are unfavorable for sampling and counting operations, and the cells need to be sampled after shaking uniformly, so that reliable results can be obtained, and the operation difficulty is increased.
The main stream of expression cell lines represented by CHO-K1 is liable to agglomerate during stationary culture.
In view of the above, it is not easy to understand why deep-well plate culture is increasingly favored.
However, the current mainstream practice has a number of disadvantages. Of these drawbacks, the two most important aspects can be summarized. On the one hand, on the equipment, the deep hole plate is not suitable for the sticking plate of the shaking table, and a deep hole plate bracket or a deep hole plate clamp needs to be refitted. Although experimenters can conveniently replace two accessories, after all, some labor is increased, and more fatigue is felt by the experimenters, the deep-hole plate is far more difficult to take and put than a shake flask, namely, the deep-hole plate is fixed to a plurality of small rubber rings, and the deep-hole plate is pulled out forcefully when taken and pushed down forcefully when put. On the other hand, the deep pore plate sold in the market is not cell culture consumable, but sample split charging consumable, and has various properties which are unfavorable for cell culture, such as no writing space on the sealing surface, narrow contact surface with the sealing plate film, easy sealing, pollution risk and the like.
The sticking board is an absolute mainstream tool for culturing cells by using the shaking bottle at present, and under most conditions, the sticking board can be installed in the shaking table, the shaking bottle can be directly placed at any position of the sticking board, the taking and placing are very convenient, the sticking is stable, and the shaking table does not have the falling risk when in operation.
In summary, each large biomedical consumable supplier does not seem to keep up with the wave of deep well plate culture, and is pushing out special products, so that the plate for separating liquid is ever more over-the-counter and barely bears the work of the culture container.
Disclosure of Invention
The utility model aims to overcome the defects of the prior art and provides a porous shaking box which is based on a deep pore plate and is suitable for cell suspension culture and is adaptive to a cell shaking table sticky plate.
In order to achieve the above purpose, design a porous shake box suitable for cell shaking table glues board, including shaking box shell, buffer chamber and cultivate the room, shake the deep hole that is equipped with a plurality of arrays on the box shell, the deep hole is used for placing extension rifle head, and deep hole first section is close to opening one side and is the buffer chamber, and deep hole second section is kept away from opening one side and is cultivateed the room, deep hole first section with be equipped with the circular arc ring between the deep hole second section, the circular arc ring is limited the intercommunication department of deep hole first section and deep hole second section to the arc and is about mouthful, and the arc is about mouthful and extension rifle head external diameter cooperation, and circular arc ring is close to deep hole opening one side and is the straight face, circular arc ring is kept away from deep hole opening one side and is the arc inclined plane.
Preferably, the shake box shell is used for being stuck on a shake table sticking plate.
Preferably, the first section of the deep hole is a cylindrical through hole and is communicated with the second section of the deep hole, and the buffer chamber of the first section of the deep hole is used for buffering cell liquid splashing.
Preferably, the top and bottom of the second section of the deep hole are conical surfaces for culturing cell sap.
Preferably, the shake box shell leaves enough space for marking of each deep hole, and the shake box shell is provided with a sealing surface matched with the sealing plate film.
Compared with the prior art, the utility model has the advantages that:
1. the lower part is enlarged, the bottom surface is flat, and the adhesive can be firmly adhered to the bonding plate of the shaking table;
2. there are enough planes for writing marks;
3. the arc ring and the buffer chamber are designed, so that the probability of splashing the culture outside the box can be reduced, and cross contamination among cell strains and worse results are avoided;
4. the deep hole plate culture device is specially designed, the size is adapted, the suction clamping position is adapted to a 1mL lengthened gun head, the deep bottoming cannot be continued, but the cell sap can be just taken out, the problem of difficult taking and placing is avoided during taking, and the labor of experimenters is reduced;
5. the integrated 24 culture spaces can simultaneously culture 24 cells, and is more suitable for high-flux cell strain screening culture or continuous subculture.
Drawings
FIG. 1 is a top view of a multiple roll cartridge provided by the present utility model;
FIG. 2 is a side view of the multiple roll cartridge provided by the present utility model;
fig. 3 is a side cross-sectional view of a multiple roll cartridge provided by the present utility model.
In the figure: 1. a shake box housing; 2. sealing surfaces; 3. deep holes; 4. arc ring; 5. arc opening; 6. a buffer chamber; 7. a culture chamber; 8. an elongated gun head.
Detailed Description
The construction and principles of the present utility model will be readily apparent to those skilled in the art from the following description taken in conjunction with the accompanying drawings. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the utility model.
The utility model provides a porous shaking box suitable for a cell shaking table sticky plate, which comprises a shaking box shell 1, a buffer chamber 6 and a culture chamber 7, wherein the essence of the utility model is an integrated shaking tube, and a proper small culture space is formed by protruding and sinking on the basis of a cuboid shape
As shown in figure 1, the enlarged bottom of the lower part of the porous shaking box shell 1 is flat and is used for supporting the whole shaking box, and the porous shaking box shell can be firmly stuck on a shaking table sticking plate to prevent from toppling over when placed on a table top. The material of the porous shaking box is consistent with that of a shaking bottle, and the porous shaking box has two options, namely Polycarbonate (PC) or polyethylene terephthalate (PETG).
As shown in fig. 2, the shake box housing 1 leaves enough plane space for marking of each deep hole 3, and the shake box housing 1 is provided with a sealing surface 1 to be matched with a sealing plate film, so that gaps are avoided from entering, and the risk of culture pollution is reduced.
As shown in fig. 1 and 3, the shake box casing 1 is provided with a plurality of two-stage deep holes 3 in array, the deep holes 3 are used for placing lengthened gun heads 8 for the entry and exit of culture solution or cells, in this embodiment, the number of the deep holes 3 is 24, and each hole is suitable for the shake suspension culture of cells with the volume of 1-5 ml.
The first section of deep hole 3 is close to opening one side and is buffer chamber 6, and cylindrical through-hole communicates with the second section of deep hole 3, and buffer chamber 6 is used for the buffering of cell sap spill, even cell sap carelessly still is splashed out by arc about mouthful 5, also can stay in buffer chamber 6, avoids worse result emergence. The arc-shaped ring 4 can provide a perfect suction clamping position for the 1mL lengthened gun head 8, and cannot go deep enough to touch the bottom, but just can take out the cell sap, because the hole for placing the gun head in the gun head box with 5 and 1mL has the same aperture, and the gun head with 1mL lengthened gun head 8 is more commonly used in cell operation.
The second section of the deep hole 3 far away from the opening is provided with a culture chamber 7, the top and the bottom of the culture chamber 7 are conical surfaces, and the culture chamber 7 is like an inner container, is a space for cell suspension culture and can be used for culturing 1-5mL of cell fluid.
An arc ring is arranged between the first section and the second section of the deep hole 3, and the arc ring limits the communication part between the first section and the second section of the deep hole 3 to an arc opening, so that the cross contamination between cell strains caused by splashing of culture in the culture chamber 7 on a sealing plate film or splashing to other holes is further prevented
The arc approximate opening is matched with the outer diameter of the lengthened gun head 8, one side, close to the opening of the deep hole 3, of the arc ring is a straight surface, and one side, far away from the opening of the deep hole 3, of the arc ring is an arc inclined surface.
The porous shaking box is very convenient to use and place, is used for cell suspension culture, has the effect similar to that of shaking, does not need to pay for the cost of the deep hole 3 plate bracket, is more convenient to sample and divide the liquid to use the deep hole 3 plate, has better transparency, is more expensive even though single, can culture 24 cells simultaneously due to the integration of 24 culture spaces, and is particularly suitable for high-flux cell strain screening culture or continuous passage culture.
In the stable study, it is required that suspension culture passage is necessary, and if 3 optimal monoclonal cell lines need to be studied, the whole study process is passed 30 times, 90 shake flasks are consumed, and 2700mL of the corresponding culture medium is consumed. By adopting the product for passage, 4mL of culture per hole is enough, only 4 porous shaking boxes are consumed, corresponding 360mL of culture medium is needed, and 4 frozen node cells still need to be amplified in shaking bottles, so that 360mL of culture medium is consumed. One round of stability study saves about 2 liters of culture medium and has a price of about 3000 yuan. 90 shake flasks are about 2000 yuan, and 4 cost of the shake flasks can be controlled within 400 yuan. Therefore, the cost of the culture medium and the consumable materials can be saved by 4600 yuan only from the research of the stability of engineering cell strains, and the application range and the application value of the small-scale suspension culture are far from the cost of the culture medium and the consumable materials.
The above description is only specific to the embodiments of the utility model, but the scope of the utility model is not limited thereto, and any person skilled in the art who is skilled in the art to which the utility model pertains shall apply to the technical solution and the novel concept according to the utility model, and shall all be covered by the scope of the utility model.
Claims (5)
1. The utility model provides a porous shake box suitable for cell table glues board, includes shakes the box shell, shake the deep hole that is equipped with a plurality of arrays on the box shell, the deep hole is used for placing extension type rifle head, its characterized in that
One side of the first section of the deep hole close to the opening is provided with a buffer chamber,
the side of the second section of the deep hole far away from the opening is a culture chamber,
the novel deep hole gun is characterized in that an arc ring is arranged between the first section of the deep hole and the second section of the deep hole, the communication position between the first section of the deep hole and the second section of the deep hole is limited to an arc opening by the arc ring, the arc opening is matched with the outer diameter of the lengthened gun head, one side, close to the opening of the deep hole, of the arc ring is a straight surface, and one side, far away from the opening of the deep hole, of the arc ring is an arc inclined surface.
2. A multi-well shake cell adapted for use in a shake well plate according to claim 1, wherein the shake cell housing is adapted to be attached to the shake well plate.
3. The multi-hole shaking box for the cell shaking table sticking plate according to claim 1, wherein the first section of the deep hole is a cylindrical through hole and is communicated with the second section of the deep hole, and the buffer chamber of the first section of the deep hole is used for buffering cell liquid splashing.
4. A multi-well shake flask suitable for use in a cell shaker plate as claimed in claim 1, wherein the top and bottom of the second section of the recess are tapered surfaces for culturing cell fluid.
5. A multi-well shake cell for a cell shaker adhesive plate as defined in claim 1 wherein the shake cell housing is provided with a sealing surface for engaging the sealing plate membrane to allow sufficient space for labeling of each well.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321038792.2U CN220034529U (en) | 2023-05-04 | 2023-05-04 | Porous shake box suitable for cell table glues board |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321038792.2U CN220034529U (en) | 2023-05-04 | 2023-05-04 | Porous shake box suitable for cell table glues board |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220034529U true CN220034529U (en) | 2023-11-17 |
Family
ID=88741955
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321038792.2U Active CN220034529U (en) | 2023-05-04 | 2023-05-04 | Porous shake box suitable for cell table glues board |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220034529U (en) |
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2023
- 2023-05-04 CN CN202321038792.2U patent/CN220034529U/en active Active
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