CN219489960U - Suspension type cell culture device - Google Patents
Suspension type cell culture device Download PDFInfo
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- CN219489960U CN219489960U CN202223343632.7U CN202223343632U CN219489960U CN 219489960 U CN219489960 U CN 219489960U CN 202223343632 U CN202223343632 U CN 202223343632U CN 219489960 U CN219489960 U CN 219489960U
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- cylinder body
- barrel
- culture
- sample injection
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Abstract
The utility model relates to a suspension type cell culture device which comprises a culture cylinder body, a stirring mechanism and a sample injection cylinder body, wherein the stirring mechanism is connected below the culture cylinder body; the upper end of the sample injection barrel is connected with an upper cover, the upper cover is connected with an I-shaped feed inlet, the lower end of the I-shaped feed inlet is communicated with the sample injection barrel, the side surface of the sample injection barrel is connected with a second feed inlet and a plurality of air inlet push valves, the air inlet of each air inlet push valve is connected with an air inlet pipeline, the air outlet of each air inlet push valve is connected with an aeration distribution device arranged in the inner cavity of the culture barrel, the upper cover is also provided with an antifoaming electrode mounting hole, and an antifoaming electrode extending into the inner cavity of the culture barrel is connected in the antifoaming electrode mounting hole. The beneficial effects of the utility model are as follows: the method has the advantages that the method is convenient for feeding different culture liquids in a time-sharing mode, the probability of mixing different culture liquids with each other is reduced, and then the culture effect on cells can be improved.
Description
Technical Field
The utility model relates to the technical field of cell culture equipment, in particular to a suspension type cell culture device.
Background
Common fermentors are divided into solid fermentors and liquid fermentors. The fermenter is divided into a seed tank (seed fermenter) and a fermenter according to the volume and the use. The fermenter is further divided into a ventilating fermenter and an anaerobic fermenter according to the type of oxygen demand. However, the sample injection of a general fermentation tank is performed through sample injection pipes on the cover body, and when different culture liquid sample injection is required, the quantity of the arranged sample injection pipes is insufficient due to the limited area of the upper surface of the cover body, so that the separation of the sample injection pipes is not easy to realize.
Disclosure of Invention
In order to overcome at least part of the defects in the prior art, the embodiment of the utility model provides a suspension type cell culture device which is simple in structure, convenient to use, convenient to feed different culture liquids in a time-sharing mode, and capable of reducing the probability of mixing different culture liquids, and further capable of improving the cell culture effect.
The utility model relates to a suspension type cell culture device which comprises a culture cylinder body, a stirring mechanism and a sample injection cylinder body, wherein the stirring mechanism is connected below the culture cylinder body, a stirring shaft of the stirring mechanism stretches into the culture cylinder body, the sample injection cylinder body is a cylindrical cylinder body with an opening at the lower end, the sample injection cylinder body is connected above the culture cylinder body, the opening at the lower end of an inner cavity of the sample injection cylinder body is communicated with the opening at the upper end of the inner cavity of the culture cylinder body, and the culture cylinder body and the sample injection cylinder body are connected with each other through a flange;
the utility model discloses a sample injection barrel, including injecting sample into barrel, injecting sample into barrel upper end is connected with the upper cover, the upper cover is connected with I type feed inlet, I shape feed inlet lower extreme with injecting sample into barrel intercommunication the side of injecting sample into barrel is connected with second feed inlet and a plurality of air inlet push valve, the air inlet of air inlet push valve is connected with the air inlet pipeline, the gas outlet of air inlet push valve is connected with the ventilation distribution device that sets up in the inner chamber of cultivating the barrel, the upper cover has still seted up defoaming electrode mounting hole in the defoaming electrode mounting hole is connected with the defoaming electrode that stretches into cultivate the inner chamber of barrel.
Further, a sampling interface is arranged on the side surface of the culture cylinder body and is communicated with the inner cavity of the culture cylinder body, and the sampling interface is connected with a double-port sampling valve for sampling liquid in the inner cavity of the culture cylinder body.
Further, the outside of cultivateing the barrel has cup jointed the cover of strengthening, the outside of strengthening the cover has cup jointed the heat preservation cover that is used for reducing the heat exchange of cultivateing liquid and external environment in the cultivation barrel.
Further, the side face of the culture cylinder body is connected with a strip-shaped perspective mirror.
Further, the upper cover and the sample injection cylinder are connected with each other through a sealing flange.
Further, the lower side surface of the culture cylinder body is connected with a plurality of electrode interfaces along the circumferential direction.
Further, the upper surface of the upper cover is also connected with a plurality of flat joints communicated with the inner cavity of the sample injection cylinder.
Further, the upper surface of upper cover is connected with the handle of being convenient for remove the upper cover, the quantity of handle is 2, 2 the handle is followed the axis symmetry setting of upper cover.
Further, a combination valve for replacing or discharging the culture liquid in the culture cylinder is connected to the lower side of the culture cylinder.
The utility model has the advantages that: the feeding of at least two kinds of culture liquids can be completed simultaneously through the I-shaped feeding port arranged on the upper cover and the second feeding port arranged on the side surface of the sample injection cylinder, so that the probability of mixing different culture liquids in advance is reduced;
in addition, through setting up the rabbling mechanism that cultivates the barrel below to cultivate the liquid in the barrel and stir, on the one hand can accelerate the mixed speed of different cultivation liquids, on the other hand, can accelerate the dissolved oxygen efficiency of cultivation liquid, improve the culture environment of cell.
The foregoing and other objects, features and advantages of the utility model will be apparent from the following more particular description of preferred embodiments, as illustrated in the accompanying drawings.
Drawings
In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the overall structure of a suspension type cell culture apparatus.
Fig. 2 is a schematic view of the internal structure of fig. 1.
FIG. 3 is a schematic view of the structure of the culture cylinder of FIG. 1.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
I-shaped feed inlet: the feed channel axis is a straight line and cross-sectional shape of the tubular feed structure is unchanged, and the cross section of the tubular feed structure comprises but is not limited to circular, rectangular and other structures.
Intake push valve: a valve for controlling the inlet of the gas, for controlling the on and off of the gas inlet channel and for controlling the inlet amount of air, in practice, a micro-amount of liquid or powder additive may also be introduced through the gas.
In a preferred embodiment of the utility model, a suspension type cell culture device comprises a culture cylinder 1, a stirring mechanism 2 and a sample injection cylinder 3, wherein the stirring mechanism 2 is connected below the culture cylinder 1, a stirring shaft of the stirring mechanism stretches into the culture cylinder 3, the sample injection cylinder 3 is a cylindrical cylinder with an opening at the lower end, the culture cylinder 1 is connected above the culture cylinder 3, the opening at the lower end of an inner cavity of the sample injection cylinder 1 is communicated with the opening at the upper end of the inner cavity of the culture cylinder 3, and the culture cylinder 1 and the sample injection cylinder 3 are connected with each other through a flange;
the upper end of the sample injection cylinder body 3 is connected with an upper cover 4, the upper cover 4 is connected with an I-shaped feed inlet 41, the lower end of the I-shaped feed inlet 41 is communicated with the sample injection cylinder body 3, the side surface of the sample injection cylinder body 3 is connected with a second feed inlet 31 and a plurality of air inlet push valves 32, air inlets of the air inlet push valves 32 are connected with air inlet pipelines, air outlets of the air inlet push valves 32 are connected with ventilation distribution devices (not shown) arranged in the inner cavity of the culture cylinder body, defoaming electrode mounting holes are further formed in the upper cover 4, and defoaming electrodes extending into the inner cavity of the culture cylinder body 1 are connected in the defoaming electrode mounting holes. Through setting up I type feed inlet 41 at the upper cover and seting up at the second feed inlet 31 of the side of injecting the barrel 3, can accomplish the feeding of at least two kinds of cultivation liquids simultaneously, reduce the probability that different cultivation liquids mix in advance, and owing to set up injecting the barrel 3, can be convenient set up more functional devices (like other I type feed inlets, air inlet and defoaming electrode etc.) on upper cover 4 or injecting the barrel 3, can also reduce the setting quantity of the functional devices of cultivating the barrel 1 side, make cultivate barrel 1 be convenient for seal or keep warm, thereby can improve the stability of the cultivation environment that cultivates the liquid construction in cultivating barrel 1. Through setting up the rabbling mechanism 2 in cultivateing barrel 1 below to cultivateing the liquid in barrel 1 and stir, on the one hand can accelerate the mixed speed of different cultivateing liquids, on the other hand can accelerate the dissolved oxygen efficiency of cultivateing the liquid, improves the culture environment of cell.
In the above embodiment, the side surface of the culture cylinder 1 is provided with the sampling port, the sampling port is communicated with the inner cavity of the culture cylinder 1, and the sampling port is connected with the double-port sampling valve 11 for sampling the liquid in the inner cavity of the culture cylinder. Through the setting of two mouthfuls of sampling valves 11, not only convenient sample, two mouthfuls of sampling valves 11 have two sample connection moreover, can directly carry out the duplicate sample, need not to follow-up adoption individual equipment and carries out the segmentation of sample, can improve sampling efficiency.
In the above embodiment, in order to improve the stability of the culture environment in the culture cylinder 1, the outer side of the culture cylinder 1 is sleeved with the reinforcing sleeve 12, and the outer side of the reinforcing sleeve 12 is sleeved with the heat-insulating jacket 13 for reducing the heat exchange between the culture liquid in the culture cylinder 1 and the external environment.
In the above embodiment, the side of the culture cylinder 1 is connected with the elongated transparent mirror 14 for facilitating the observation of the culture liquid in the culture cylinder 1 for timely knowing the status of the culture liquid.
In the above embodiment, in order to improve the stability and reliability of the connection between the upper cover 4 and the sample introduction cylinder 3, the upper cover 4 and the sample introduction cylinder 3 are connected to each other by the sealing flange 5.
In the above embodiment, in order to improve the expandability of the culture cylinder 1, the lower side surface of the culture cylinder 1 is connected with a plurality of electrode ports 15 in the circumferential direction. The electrode interface is DO electrode interface or pH electrode interface.
In the above embodiment, in order to facilitate introduction of more different kinds of culture liquids, a plurality of flat connectors 42 communicating with the inner cavity of the sample injection cylinder 3 are further connected to the upper surface of the upper cover 4.
In the above embodiment, the upper surface of the upper cover 4 is connected with the handles 43 that facilitate moving the upper cover, the number of the handles 43 is 2, and the 2 handles 43 are symmetrically arranged along the central axis of the upper cover.
In the above embodiment, a combination valve 16 for changing or discharging the culture liquid in the culture cylinder is connected to the lower side of the culture cylinder 1.
The principles and embodiments of the present utility model have been described in detail with reference to specific examples, which are provided to facilitate understanding of the method and core ideas of the present utility model; meanwhile, as those skilled in the art will have variations in the specific embodiments and application scope in accordance with the ideas of the present utility model, the present description should not be construed as limiting the present utility model in view of the above.
Claims (9)
1. A suspension cell culture apparatus, characterized in that: the device comprises a culture cylinder body, a stirring mechanism and a sample injection cylinder body, wherein the stirring mechanism is connected below the culture cylinder body, a stirring shaft of the stirring mechanism stretches into the culture cylinder body, the sample injection cylinder body is a cylindrical cylinder body with an opening at the lower end, the sample injection cylinder body is connected above the culture cylinder body, the opening at the lower end of an inner cavity of the sample injection cylinder body is communicated with the opening at the upper end of the inner cavity of the culture cylinder body, and the culture cylinder body and the sample injection cylinder body are connected with each other through a flange;
the utility model discloses a sample injection barrel, including injecting sample into barrel, injecting sample into barrel upper end is connected with the upper cover, the upper cover is connected with I shape feed inlet, I shape feed inlet lower extreme with injecting sample into barrel intercommunication the side of injecting sample into barrel is connected with second feed inlet and a plurality of air inlet push valve, the air inlet of air inlet push valve is connected with the air inlet pipeline, the gas outlet of air inlet push valve is connected with the distribution device that ventilates that sets up in the inner chamber of cultivating the barrel, still set up defoaming electrode mounting hole on the upper cover defoaming electrode mounting hole is connected with the defoaming electrode that stretches into cultivate the inner chamber of barrel.
2. The suspension cell culture apparatus of claim 1 wherein: the side of cultivateing the barrel has seted up the sampling interface, the sampling interface with cultivate the inner chamber intercommunication of barrel, the sampling interface is connected with and is used for right liquid in cultivateing the inner chamber of barrel carries out the double-port sampling valve of sample.
3. The suspension cell culture apparatus of claim 1 wherein: the outside of cultivateing the barrel has cup jointed the cover of strengthening the outside of cover has cup jointed the heat preservation cover that is used for reducing the heat exchange of cultivateing liquid and external environment in the cultivateing the barrel.
4. The suspension cell culture apparatus of claim 1 wherein: the side of the culture cylinder body is connected with a strip-shaped perspective mirror.
5. The suspension cell culture apparatus of claim 1 wherein: the upper cover is connected with the sample injection cylinder body through a sealing flange.
6. The suspension cell culture apparatus of claim 1 wherein: the lower side of the culture cylinder body is connected with a plurality of electrode interfaces along the circumferential direction.
7. The suspension cell culture apparatus of claim 1 wherein: the upper surface of the upper cover is also connected with a plurality of flat joints which are communicated with the inner cavity of the sample injection cylinder body.
8. The suspension cell culture apparatus of claim 1 wherein: the upper surface of upper cover is connected with the handle of being convenient for remove the upper cover, the quantity of handle is 2, 2 the handle is followed the axis symmetry setting of upper cover.
9. The suspension cell culture apparatus of claim 1 wherein: the lower side of the culture cylinder body is connected with a combination valve for replacing or discharging the culture liquid in the culture cylinder body.
Priority Applications (1)
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CN202223343632.7U CN219489960U (en) | 2022-12-14 | 2022-12-14 | Suspension type cell culture device |
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CN202223343632.7U CN219489960U (en) | 2022-12-14 | 2022-12-14 | Suspension type cell culture device |
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CN219489960U true CN219489960U (en) | 2023-08-08 |
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CN202223343632.7U Active CN219489960U (en) | 2022-12-14 | 2022-12-14 | Suspension type cell culture device |
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