CN219194972U

CN219194972U - Automatic nucleic acid extraction device

Info

Publication number: CN219194972U
Application number: CN202222917348.XU
Authority: CN
Inventors: 钟玙沄; 陈斌
Original assignee: Guangzhou Huabo Biopharmaceutical Research Institute
Current assignee: Guangzhou Huabo Biopharmaceutical Research Institute
Priority date: 2022-11-02
Filing date: 2022-11-02
Publication date: 2023-06-16
Anticipated expiration: 2032-11-02

Abstract

The utility model discloses an automatic nucleic acid extraction device, which comprises a temperature adjusting device, a reagent mixing device, a centrifugal device and a control device; the reagent mixing device is internally provided with a test tube, and can drive the test tube to move so as to complete the mixing of the reagents. The temperature adjusting device is arranged in the reagent mixing device and comprises a temperature sensor, a heating device and a heat radiating device, the heating device can heat the test tube, the sample to be tested can be cracked at high temperature, and nucleic acid capturing is completed in the reagent mixing process. And then the test tube in the reagent mixing device is subjected to centrifugal treatment through the centrifugal device, so that the reagent in the test tube is separated under the action of centrifugal force, the separation and purification process of nucleic acid is completed, then the heat is dissipated through the heat dissipation device, finally the test tube is taken out, and the water layer below the test tube is separated, so that a pure nucleic acid template can be obtained, and the extraction process is completed. The device is simple and convenient to use and high in extraction efficiency.

Description

Automatic nucleic acid extraction device

Technical Field

The utility model relates to the technical field of nucleic acid detection pretreatment, in particular to an automatic nucleic acid extraction device.

Background

Nucleic acid diagnosis has three main steps: 1. extracting nucleic acid from the biological sample to obtain a nucleic acid template; 2. performing an amplification reaction on the nucleic acid template; 3. and detecting the signals to obtain corresponding detection results. The acquisition of a nucleic acid template is the first step in the overall nucleic acid diagnostic process and is also the basis for conducting nucleic acid analysis. At present, two nucleic acid extraction modes of manual extraction and automatic extraction exist. The manual extraction requires simple materials, but human errors are often brought into the extraction result, and the extraction yield and purity of DNA are different when the extraction is repeated, so that downstream biological experiments and diagnostic detection are affected. Therefore, the laboratory is urgent to realize the automation of nucleic acid extraction, and the automation of the extraction can greatly improve the yield, purity and repeatability of DNA extraction, so that the subsequent detection process is orderly carried out, and the method has positive significance for the diagnosis of diseases of large samples and the countermeasure of sudden infectious diseases.

The full-automatic nucleic acid extractor is one kind of nucleic acid extracting reagent to complete the fast extraction of sample nucleic acid automatically. The centrifugal column method full-automatic nucleic acid extractor mainly integrates functional components such as a centrifugal machine, an automatic pipetting device, an automatic gripper and the like, and the maximum flux of the centrifugal column method full-automatic nucleic acid extractor is 12 samples. The implementation principle is as follows: fixing silica gel membrane in centrifuge tube, allowing liquid to pass through silica gel membrane by centrifugal force or negative pressure, allowing nucleic acid to remain on the membrane, digesting with protease, washing with rinsing solution to remove protein, lipid, etc., and eluting with purified solution to obtain nucleic acid. The method has the advantages of simple principle, rapidness and high extraction quality; however, the method has the defects of inconvenient use, no advantage over manual extraction, and only specified consumables.

Disclosure of Invention

In order to overcome the defects of the prior art, the utility model aims to provide an automatic nucleic acid extraction device which integrates a centrifugal device, a reagent mixing device and a temperature adjusting device into a whole, integrates the effect of nucleic acid extraction, has high efficiency and greatly shortens the whole nucleic acid extraction reaction time.

The utility model adopts the following technical scheme:

an automatic nucleic acid extraction device comprises a temperature adjusting device, a reagent mixing device, a centrifugal device and a control device; wherein the centrifugal device is arranged at the bottom of the reagent mixing device; the temperature adjusting device is arranged in the reagent mixing device; the temperature adjusting device comprises a temperature sensor, a heating device and a heat radiating device; the reagent mixing device is internally provided with a test tube; the centrifugal device, the temperature adjusting device and the reagent mixing device are electrically connected with the control device.

Further, the centrifugal device comprises a bottom plate, a bracket, a centrifugal motor, a coupler, an extension shaft and a turntable; the centrifugal motor is arranged on the bracket, the bracket is fixed on the bottom plate, an output shaft of the centrifugal motor is connected with the extension shaft through a coupler, and the turntable is connected with the extension shaft; the turntable is arranged at the bottom of the reagent mixing device; the centrifugal motor is electrically connected with the control device.

Further, the reagent mixing device comprises a shell, a test tube fixing device and a mixing motor; the test tube fixing device and the mixing motor are arranged in the shell, and test tube accommodating holes matched with test tube sizes are formed in the test tube fixing device; the hybrid motor is connected with the test tube fixing device, and the hybrid motor is electrically connected with the control device.

Further, the shell is provided with a cover, and a rubber strip matched with the test tube in size is arranged on the cover.

Further, the temperature sensor is arranged at the bottom end of the test tube fixing device.

Further, the heating devices are respectively arranged on two sides of the test tube fixing device, and the test tube accommodating holes are arranged in a space formed by the heating devices.

Further, the heating device is a silica gel etched resistor film.

Further, the heat dissipation device is arranged on the shell and is placed in parallel with the test tube fixing device.

Further, the heat dissipating device is a fan.

Further, a plurality of ventilation and heat dissipation holes are formed in the shell.

Compared with the prior art, the utility model has the beneficial effects that:

(1) The utility model discloses an automatic nucleic acid extraction device, which is an extraction device integrating a centrifugal device, a reagent mixing device and a temperature adjusting device on the basis of aqueous two-phase nucleic acid extraction. The reagent mixing device is internally provided with a test tube, and can drive the test tube to move so as to complete the mixing of the reagents. The temperature adjusting device is arranged in the reagent mixing device and comprises a temperature sensor, a heating device and a heat radiating device, the heating device can heat the test tube, the sample to be tested can be cracked at high temperature, and nucleic acid capturing is completed in the reagent mixing process. And then the test tube in the reagent mixing device is subjected to centrifugal treatment through the centrifugal device, so that the reagent in the test tube is separated under the action of centrifugal force, the separation and purification process of nucleic acid is completed, then the heat is dissipated through the heat dissipation device, finally the test tube is taken out, and the water layer below the test tube is separated, so that a pure nucleic acid template can be obtained, and the extraction process is completed. The device is simple and convenient to use and high in extraction efficiency.

(2) Specifically, the mixing motor arranged in the reagent mixing device can drive the test tube to be turned upside down, so that the reagent in the test tube is uniformly mixed; the heating device of the temperature adjusting device is a silica gel etching resistor film, the heat radiating device is a fan, the temperature change is detected through the temperature sensor, signals are fed back to the control device, and then the temperature of the reagent is reduced by the fan through an air heat convection mode.

Drawings

FIG. 1 is a schematic diagram of a reagent mixing apparatus according to the present utility model;

FIG. 2 is a schematic diagram of the structure of the test tube fixing device and the temperature adjusting device;

in the figure: 1. a reagent mixing device; 11. a housing; 12. test tube fixing device; 121. a test tube accommodation hole; 13. a hybrid motor; 2. a centrifugal device; 21. a bottom plate; 22. a bracket; 23. a centrifugal motor; 24. a coupling; 25. an extension shaft; 26. a turntable; 3. a heating device; 4. a heat sink.

Detailed Description

The present utility model will be further described with reference to the accompanying drawings and detailed description, wherein it is to be understood that, on the premise of no conflict, the following embodiments or technical features may be arbitrarily combined to form new embodiments.

As shown in fig. 1 to 2, the present utility model provides an automatic nucleic acid extraction device comprising a temperature adjustment device, a reagent mixing device 1, a centrifugation device 2, and a control device; wherein the centrifugal device 2 is arranged at the bottom of the reagent mixing device 1; the temperature adjusting device is placed in the reagent mixing device 1; the temperature adjusting device comprises a temperature sensor, a heating device 3 and a heat radiating device 4; the reagent mixing device 1 is internally provided with a test tube; the centrifuge 2, the temperature adjusting device and the reagent mixing device 1 are electrically connected to a control device.

Wherein, the centrifugal device 2 comprises a bottom plate 21, a bracket 22, a centrifugal motor 23, a coupler 24, an extension shaft 25 and a turntable 26; the centrifugal motor 23 is arranged on the bracket 22, the bracket 22 is fixed on the bottom plate 21, an output shaft of the centrifugal motor 23 is connected with the extension shaft 25 through the coupler 24, and the turntable 26 is connected with the extension shaft 25; the turntable 26 is arranged at the bottom of the reagent mixing device 1; the centrifugal motor 23 is electrically connected to the control device. The centrifugation device 2 is mainly used for reagent centrifugation, purification of nucleic acid molecules, and support of other devices mounted on the centrifugal turntable 26. The reagent mixing device 1 and the temperature adjusting device also need to perform a circular motion simultaneously during centrifugation. The centrifugal rotor 26 is locked with the extension shaft 25. In the function realization process, the output shaft of the centrifugal motor 23 starts to rotate along the axis, and the shaft coupler 24 drives the extension shaft 25, so as to drive the centrifugal turntable 26 to rotate. In the working process of the centrifugal motor 23, the base plate 21, the bracket 22 and the main body of the centrifugal motor 23 are fixed, and the centrifugal turntable 26, the extension shaft 25, the coupler 24 and the output shaft of the centrifugal motor 23 do circular motion relative to the base plate 21.

Further, the reagent mixing apparatus 1 includes a housing 11, a test tube fixing device 12, and a mixing motor 13; test tube fixing device 12 and hybrid motor 13 all set up inside casing 11, and test tube fixing device 12's inside is equipped with test tube accommodation hole 121 with test tube size assorted, and after the test tube inserted, guaranteed to have certain damping between test tube outer wall and the test tube accommodation hole 121. The test tube holder is provided with 4 test tube accommodation holes 121 at intervals, which are sequentially arranged along a straight line, thereby realizing an operation of nucleic acid extraction for a plurality of groups of reagents to improve efficiency. The hybrid motor 13 is connected with the test tube fixing device 12, and the hybrid motor 13 is electrically connected with the control device. In the mixing process, a test tube containing a sample and a double-aqueous-phase nucleic acid extraction system is placed in a test tube fixing device, the axis of the test tube is perpendicular to the mixing axis and is inclined relative to the vertical axis, and the distance between the bottom of the test tube and the mixing axis is greater than the distance between the top of the test tube and the mixing axis. After the mixing motor 13 works, the test tube follows the test tube fixing device to rotate around the mixing axis, the linear speed of the test tube follows the component of the vertical direction, the horizontal position of the test tube can be changed, when the horizontal position of the bottom of the test tube is higher than that of the top of the test tube, the reagent in the test tube can be reversed along the axis direction of the test tube, the reagent is ensured to have sufficient movement space in the test tube, the reagent reversing process is repeated for a plurality of times, and reagent mixing can be realized.

The shell 11 is provided with a cover, and a rubber strip matched with the test tube in size is arranged on the cover. When the cover rotates to the position of the closed shell 11, the rubber strip is in contact with the test tube cover to generate certain deformation, and pressure is continuously applied to the test tube cover, so that a good sealing effect is ensured to be generated inside the test tube.

The temperature sensor is arranged at the bottom end of the test tube fixing device.

The heating device 3 is a silica gel etching resistor film, and the heating device 3 is respectively arranged on two sides of the test tube fixing device and clamps the test tube accommodating hole 121 in the middle. The test tube fixing device is made of metal aluminum with good heat conductivity, corresponding grooves are designed on the front end face and the rear end face, the silica gel resistor film is respectively embedded in the two grooves in an etching mode, and the test tube accommodating hole 121 is clamped in the middle. The silica gel resistor film with symmetrical double sides can improve the temperature rising rate.

The heat dissipation device 4 is arranged on the shell 11 and is placed in parallel with the test tube fixing device. Specifically, the heat dissipating device 4 is a fan, and an air inlet and an air outlet of the fan are reserved on the housing 11. Besides the heat dissipation of the fan system, a plurality of ventilation heat dissipation holes which are arranged side by side are formed in one side of the surface of the shell 11, so that the heat dissipation effect is further improved.

The application method of the device comprises the following steps: the sample to be tested and the aqueous two-phase nucleic acid extraction system are placed in a test tube, which is inserted into the test tube accommodating hole 121 and locked with a rubber strip. The heating control is carried out by applying voltage to the two ends of the silica gel resistor film, and the data are collected by the temperature sensor, so that a closed loop with temperature control is formed. After the set temperature is reached, the mixing motor 13 drives the test tube to be turned upside down so as to enable the reagents in the test tube to reach the aim of mixing. The heating control and the mixing control can be simultaneously carried out, and the combined nucleic acid is captured while the nucleic acid is released by cracking, so that the whole nucleic acid extraction reaction time is greatly shortened. After mixing, the cooling fan is started simultaneously, the temperature of the reagent in the test tube is reduced, and preparation is made for purifying nucleic acid. And then the centrifugal motor 23 drives the mixing module and the heating module to centrifuge together, so that the reagents in the test tube can be separated under the action of centrifugal force. And after the whole process is finished, the extraction of the sample nucleic acid is finished. And taking out the test tube, and separating a water layer below the test tube to obtain the pure nucleic acid template.

The above embodiments are only preferred embodiments of the present utility model, and the scope of the present utility model is not limited thereto, but any insubstantial changes and substitutions made by those skilled in the art on the basis of the present utility model are intended to be within the scope of the present utility model as claimed.

Claims

1. An automatic nucleic acid extraction device is characterized by comprising a temperature adjusting device, a reagent mixing device, a centrifugal device and a control device; wherein the centrifugal device is arranged at the bottom of the reagent mixing device; the temperature adjusting device is arranged in the reagent mixing device; the temperature adjusting device comprises a temperature sensor, a heating device and a heat radiating device; the reagent mixing device is internally provided with a test tube; the centrifugal device, the temperature adjusting device and the reagent mixing device are electrically connected with the control device.

2. The automatic nucleic acid extraction apparatus of claim 1, wherein the centrifugation device comprises a bottom plate, a support, a centrifugal motor, a coupling, an extension shaft, and a turntable; the centrifugal motor is arranged on the bracket, the bracket is fixed on the bottom plate, an output shaft of the centrifugal motor is connected with the extension shaft through a coupler, and the turntable is connected with the extension shaft; the turntable is arranged at the bottom of the reagent mixing device; the centrifugal motor is electrically connected with the control device.

3. The automatic nucleic acid extraction apparatus of claim 1, wherein the reagent mixing apparatus comprises a housing, a test tube fixing apparatus, and a mixing motor; the test tube fixing device and the mixing motor are arranged in the shell, and test tube accommodating holes matched with test tube sizes are formed in the test tube fixing device; the hybrid motor is connected with the test tube fixing device, and the hybrid motor is electrically connected with the control device.

4. The automatic nucleic acid extraction apparatus as claimed in claim 3, wherein the housing is provided with a cover provided with a rubber strip matching the size of the test tube.

5. The automatic nucleic acid extraction device of claim 3, wherein the temperature sensor is disposed at a bottom end of the test tube holding device.

6. The automatic nucleic acid extraction apparatus as claimed in claim 3, wherein the heating means are provided on both sides of the tube fixing means, respectively, and the tube receiving hole is provided in a space formed by the heating means.

7. The automatic nucleic acid extraction apparatus according to claim 1 or 6, wherein the heating means is a silica gel etched resistive film.

8. The automatic nucleic acid extraction apparatus as claimed in claim 3, wherein the heat sink is provided on the housing and placed in parallel with the cuvette holder.

9. The automatic nucleic acid isolation apparatus according to claim 1 or 3, wherein the heat sink is a fan.

10. The automatic nucleic acid extraction device as claimed in claim 3, wherein the housing is provided with a plurality of ventilation and heat dissipation holes.