CN218026009U

CN218026009U - Nucleic acid extraction device

Info

Publication number: CN218026009U
Application number: CN202221998760.2U
Authority: CN
Inventors: 贾丽; 浦敩筱; 方迅
Original assignee: South China Normal University
Current assignee: South China Normal University
Priority date: 2022-07-29
Filing date: 2022-07-29
Publication date: 2022-12-13
Anticipated expiration: 2032-07-29

Abstract

The utility model relates to a nucleic acid extraction instrument technical field, concretely relates to nucleic acid extraction device, including base, drive module, sample module and support, drive module includes that telescopic link, micro motor and word press from both sides, and sample module and support are installed respectively on the base, and drive module installs at the support, and the telescopic link is installed at the support, and micro motor installs at the telescopic link lower extreme, and the output shaft at micro motor is installed to the word clamp. The nucleic acid extraction device can automatically complete the process of extracting nucleic acid by paper base, optimizes the extraction time of the paper base nucleic acid and improves the extraction efficiency.

Description

Nucleic acid extraction device

Technical Field

The utility model relates to a nucleic acid extraction instrument technical field, concretely relates to nucleic acid extraction device.

Background

Nucleic acid extraction is a necessary technical means for molecular biology research, and is widely applied in many fields. The currently used DNA extraction methods include phenol-chloroform method, silicon substrate-based solid phase extraction method and magnetic material-based magnetic solid phase extraction method. The phenol-chloroform method needs toxic and harmful reagents, and has more complex operation steps and longer time consumption; the silicon matrix-based solid phase extraction method involves a multistep pipetting centrifugation operation, and the shearing force formed in the centrifugation step can damage the integrity of DNA; although the magnetic solid phase extraction method based on magnetic materials can realize the automation of DNA extraction, the solid-liquid separation requires an external magnetic field, which undoubtedly increases the extraction cost. Therefore, the development of a low-cost automated DNA extraction apparatus is still necessary.

The paper-based nucleic acid extraction method can conveniently realize the extraction of DNA by modifying the functionalized material on the paper base. The existing paper-based nucleic acid extraction device is mostly extracted by a microfluidic porous disc, has a microporous structure, can automatically pump liquid by capillary force, and is easy to realize liquid flow control and detection time control. But has the defects of complex operation, long time consumption, no liquid storage and the like, can not realize rapid, simple and portable nucleic acid extraction, and is not suitable for disease diagnosis in resource-deficient areas.

SUMMERY OF THE UTILITY MODEL

To the technical problem who exists among the prior art, the utility model aims at: the nucleic acid extraction device can automatically complete the process of extracting nucleic acid by paper base, optimizes the extraction time of the paper base nucleic acid and improves the extraction efficiency.

In order to achieve the above purpose, the utility model adopts the following technical scheme: the utility model provides a nucleic acid extraction element, includes base, drive module, sample module and support, and drive module includes telescopic link, micro motor and a word clamp, and sample module and support are installed respectively on the base, and drive module installs at the support, and the telescopic link is installed at the support, and micro motor installs at the telescopic link lower extreme, and the output shaft at micro motor is installed to a word clamp.

Further, the sample module includes sample seat, sample dish and step motor, and the sample seat is installed on the base, and the step motor mounting groove has been seted up to the sample seat, and step motor installs at the step motor mounting groove, and the output shaft at step motor is installed to the sample dish, and the sample dish is opened and is equipped with the sample pipe groove.

Furthermore, a magnet is installed on the side wall of the sample tube groove, and a Hall sensor is installed at a position corresponding to the support.

Further, still include control module, control module includes development board, drive plate and rechargeable battery, and the installation cavity has been seted up to the base, and development board, drive plate and rechargeable battery all install at the installation cavity.

Furthermore, the base is provided with a film key.

Further, the support includes bracing piece and connecting rod, and the bracing piece is vertical to be installed at the base, and the one end of connecting rod is installed at the bracing piece, and the other end at the connecting rod is installed to the telescopic link.

Generally speaking, the utility model has the advantages that: (1) The utility model discloses a drive module's design can accomplish paper base automatically and draw absorption, washing, elution overall process in the nucleic acid, has optimized paper base nucleic acid and has drawed the time, has improved paper base nucleic acid and has drawed efficiency.

(2) The utility model discloses a be provided with a word and press from both sides and a plurality of sample tube groove, detection personnel only need contact uncontaminated sample cell and paper base, can effectively avoid the contact of detection personnel and sample to reduce the possibility that detection personnel is infected in the testing process.

(3) The utility model discloses an automatic change nucleic acid extraction element can avoid the human error that testing personnel caused in the experiment operation process to experimental data's reproducibility and reliability have been guaranteed.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a partial enlarged view of the sample module of the present invention.

Wherein: 1 is the base, and 2 is the support, and 3 are the telescopic link, and 4 are micro motor, and 5 are the word clamp, and 6 are the sample seat, and 7 are the sample dish, and 8 are the sample tube groove, and 9 are magnet, and 10 are hall sensor, 11 are the installation cavity, and 12 are step motor.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.

As shown in figures 1-2, a nucleic acid extraction device, which comprises a base 1, drive module, sample module and support 2, drive module includes telescopic link 3, micro motor 4 and word clamp 5, sample module and support 2 are installed respectively on base 1, drive module installs at support 2, telescopic link 3 is installed at support 2, micro motor 4 installs the lower extreme at telescopic link 3, the output shaft at micro motor 4 is installed to word clamp 5, be used for the centre gripping paper base, in this embodiment, support 2 includes bracing piece and connecting rod, the bracing piece is vertical to be installed at the base, the one end of connecting rod is installed at the bracing piece, telescopic link 3 installs the other end at the connecting rod, telescopic link 3 has two, the horizontally sets up on the bracing piece, the stability of telescopic link has been guaranteed.

Specifically, the sample module includes sample holder 6, sample dish 7 and step motor 12, and sample holder 6 is installed on base 1, and the step motor mounting groove has been seted up to sample holder 6, and step motor 12 is installed in the step motor mounting groove, and the output shaft at step motor 12 is installed to sample dish 7, and sample pipe groove 8 has been seted up to sample dish 7. In this embodiment, four sample tube slots 8 are formed in the sample tray 7, sample tubes containing samples can be inserted into the sample tube slots 8, and the tube openings of the sample tubes can be opposite to the linear clamps 5 by rotating the sample tray 7, so that the linear clamps 5 can extend into the sample tubes.

Meanwhile, a magnet 9 is installed on the side wall of the sample tube groove 8, a Hall sensor 10 is installed at the corresponding position of the support 2 through a screw, the Hall sensor 10 can sense the magnet 9, and further the position of the sample tube is sensed, so that the sample tube is located under the linear clamp 5.

This nucleic acid extraction element still includes control module, control module includes the development board, drive plate and rechargeable battery, installation cavity 11 has been seted up to base 1, the development board, drive plate and rechargeable battery are all installed in installation cavity 11, in this embodiment, base 1 is equipped with the film button, can be used to set up nucleic acid extraction element's function, the development board is main control unit, a signal for reading film keyboard and step motor 12, telescopic link 3 and micro motor 4, and make the feedback to the signal and carry out corresponding control, the drive plate is connected with step motor 12, a speed governing is twisted on continuously to step motor 12 for driving step motor 12, in this embodiment, install the baffle between sample seat 6 and the base 1, base 1 can be sealed to the baffle, separate base 1 and sample seat 6, prevent the interference, the baffle is installed in the rechargeable battery outside, a protection is sealed for with rechargeable battery.

The utility model discloses a work flow does: putting a sample solution into a sample tube, placing the sample tube in a sample tube groove, clamping a paper base on a linear clamp, and selecting different working modes of the nucleic acid extraction device through a film key;

selecting an adsorption mode through a thin-film keyboard, starting a stepping motor to drive a sample disc to rotate, after a Hall sensor identifies a corresponding magnet, stopping the rotation of the sample disc, driving a micro motor and a linear clamp to extend downwards through a telescopic rod, enabling a linear clamp paper base to reach the bottom of a sample tube, starting the micro motor to enable the linear clamp to rotate, rotating and stirring the paper base in the sample tube, after 10 minutes, stopping the rotation of the micro motor, driving the micro motor, the linear clamp and the paper base clamped by the linear clamp to contract upwards to leave the sample tube through the telescopic rod, starting the stepping motor to drive a rotary disc and a magnet on the rotary disc to rotate, after the rotary disc and the paper base are far away from the Hall sensor, stopping the rotation of the stepping motor, and finishing the adsorption process of the device;

then a washing mode is selected through a thin film keyboard, the stepping motor drives the sample disc to rotate after being started, the Hall sensor identifies a corresponding magnet, the stepping motor stops rotating, the telescopic rod drives the micro motor and the linear clamp to synchronously and vertically extend downwards, when the paper base clamped by the linear clamp reaches the bottom of the sample tube, the micro motor starts the rotating shaft to drive the paper base to rotate and stir in the sample tube, after 3 minutes, the micro motor stops rotating, the telescopic rod drives the micro motor, the linear clamp and the paper base clamped by the linear clamp to upwards contract and leave the sample tube, then the paper base stays for 2 minutes, after the paper base is dried, the stepping motor is started to drive the sample disc and the magnet to rotate until the sample disc and the magnet are separated from the Hall sensor, and the washing process of the device is finished;

then a film keyboard is used for selecting a re-washing mode, the stepping motor is started to drive the sample disc to rotate, the Hall sensor identifies a corresponding magnet, the stepping motor stops rotating, the telescopic rod drives the micro motor and the linear clamp to synchronously and vertically extend downwards, when the paper base clamped by the linear clamp reaches the bottom of the sample tube, the micro motor is started to drive the paper base to rotate and stir in the sample tube, after 3 minutes, the micro motor stops rotating, the telescopic rod drives the micro motor, the linear clamp and the paper base clamped by the linear clamp to contract upwards to leave the sample tube, the paper base stays for 2 minutes to dry the paper base, the stepping motor is started to drive the sample disc and the magnet to rotate until the micro motor is separated from the Hall sensor, the stepping motor stops rotating, and the re-washing process of the device is finished;

then an elution mode is selected through a film keyboard, the stepping motor is started to drive a sample to spiral, the Hall sensor starts to identify the magnet, after the magnet is identified, the stepping motor stops rotating, the telescopic rod drives the micro motor and the linear clamp to extend downwards, when the paper base clamped by the linear clamp reaches the bottom of the sample tube, the micro motor is started to drive the paper base to rotate and stir in the sample tube, after 10 minutes of elution, the micro motor stops rotating, the telescopic rod drives the micro motor, the linear clamp and the paper base clamped by the linear clamp to upwards leave the sample tube, the telescopic rod stops working, meanwhile, the stepping motor is started to drive the turntable and the magnet to rotate until the turntable and the magnet are separated from the Hall sensor, the stepping motor stops rotating, and the elution process of the device is finished.

In general, the utility model can automatically complete the whole processes of adsorption, washing and elution in the process of extracting nucleic acid from paper base through the design of the driving module, thereby optimizing the extraction time of the paper base nucleic acid and improving the extraction efficiency of the paper base nucleic acid; the utility model is provided with the linear clamp and the plurality of sample tube grooves, so that the detection personnel only need to contact the sample tubes and the paper base which are not polluted, and the contact between the detection personnel and the samples can be effectively avoided, thereby reducing the possibility that the detection personnel are infected in the detection process; the utility model discloses an automatic change nucleic acid extraction element can avoid the human error that testing personnel caused in the experiment operation process to experimental data's reproducibility and reliability have been guaranteed.

The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be equivalent replacement modes, and all are included in the scope of the present invention.

Claims

1. The utility model provides a nucleic acid extraction element, its characterized in that includes base, drive module, sample module and support, and drive module includes telescopic link, micro motor and word clamp, and sample module and support are installed respectively on the base, and drive module installs at the support, and the telescopic link is installed at the support, and micro motor installs at the telescopic link lower extreme, and the output shaft at micro motor is installed to the word clamp.

2. The nucleic acid extraction device of claim 1, wherein the sample module comprises a sample holder, a sample tray and a stepping motor, the sample holder is mounted on the base, the sample holder is provided with a stepping motor mounting groove, the stepping motor is mounted on the stepping motor mounting groove, the sample tray is mounted on an output shaft of the stepping motor, and the sample tray is provided with a sample tube groove.

3. The nucleic acid extracting apparatus according to claim 2, wherein the magnet is mounted on the side wall of the sample tube slot, and the hall sensor is mounted at a position corresponding to the support.

4. The nucleic acid extraction device according to claim 1, further comprising a control module, wherein the control module comprises a development board, a driving board and a rechargeable battery, the base is provided with a mounting cavity, and the development board, the driving board and the rechargeable battery are all mounted in the mounting cavity.

5. The apparatus for extracting nucleic acid as claimed in claim 4, wherein the base is provided with a membrane key.

6. The nucleic acid extraction apparatus of claim 1, wherein the support comprises a support rod and a connecting rod, the support rod is vertically mounted on the base, one end of the connecting rod is mounted on the support rod, and the telescopic rod is mounted on the other end of the connecting rod.