CN214991590U

CN214991590U - Nucleic acid hybridization experimental apparatus

Info

Publication number: CN214991590U
Application number: CN202022739664.3U
Authority: CN
Inventors: 王晓燕
Original assignee: Shanghai Ya Jian Medical Technology Co ltd
Current assignee: Shanghai Ya Jian Medical Technology Co ltd
Priority date: 2020-11-24
Filing date: 2020-11-24
Publication date: 2021-12-03
Anticipated expiration: 2030-11-24

Abstract

The utility model discloses a nucleic acid hybridization experimental apparatus, comprises a laboratory bench, the centre of laboratory bench is provided with the recess, the top fixed mounting of recess has analytic device, analytic device includes solid fixed ring, gu fixed ring's axle center department fixedly connected with bearing, the axle center department fixedly connected with column spinner of bearing, the analytic dish of top fixedly connected with of column spinner, the top of analytic dish is provided with puts into the check. The utility model discloses utilize analytic device's analytic dish positive and negative rotation oscillation and high-speed rotation to move liquid, removed traditional reaction unit from and relied on long basin oscillation reaction liquid, the numerous and complex operation that the straw moved liquid, can change the rotational speed through the motor and control slow speed oscillation and quick centrifugal step, and the storage bottle can pour into reagent in advance the utility model discloses simple structure simple operation has realized centrifugal dehydration and has moved the mesh of liquid, but wide application in nucleic acid molecule hybridization experiment.

Description

Nucleic acid hybridization experimental apparatus

Technical Field

The utility model relates to a nucleic acid hybridization technical field specifically is a nucleic acid hybridization experimental apparatus.

Background

The process by which complementary nucleotide sequences form stable hybrid double-stranded DNA molecules by Walson-Crick base pairing is called hybridization, and nucleic acid molecule hybridization is an essential molecular biology experiment. The general procedure for hybridization of nucleic acid molecules is to determine the detection direction, prepare the membrane strip and reagent for the test, immerse the membrane strip in the reaction device of the nucleic acid molecule hybridization apparatus, generally, in the membrane tank containing the hybridization solution, then add the experimental sample obtained by amplification in the PCR amplification apparatus into the hybridization solution, mix it with the reagent and carry out the pre-reaction of specific binding with the molecular probe on the membrane strip, then add quantitative binding solution, eluent, and developing solution in sequence and at regular time, the membrane tank should still oscillate continuously during the whole reaction process to ensure complete infiltration of the membrane strip, the reagent and the experimental sample are contacted and reacted uniformly, and meanwhile, the reaction process should be kept at the designated temperature (usually higher than the ambient temperature). And after the reaction is finished, removing the reagent, immersing the membrane strip into the termination solution, taking out the membrane strip after the reaction is finished and the color development and the shaping are finished, scanning the membrane strip by using a scanner, and automatically archiving the result.

The traditional nucleic acid molecule hybridizing device adopts a long water tank type heating reaction device. Specifically, be provided with a plurality of membrane tank in a long basin, required experimental temperature relies on letting in warm water in the long basin, then heats the warm water to appointed experimental temperature: in order to make the reagent and the experimental sample react uniformly, the oscillation action is needed, and the shaking of the long water tank drives the membrane tank to move; the steps of liquid adding and liquid transferring are respectively inserted into each membrane groove for liquid adding or liquid absorbing by depending on a suction pipe of the instrument. However, in this method, since the pipetting step is performed using a pipette, the respective operations must be performed separately, which causes a deviation in the reaction time of the same lot of test samples, and the operation may be performed for 3 hours, which causes an error in the test result, a new reaction apparatus is required, which allows the reagent to be pipetted quickly, and which has high operability.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a nucleic acid hybridization experimental apparatus to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a nucleic acid hybridization experimental apparatus, includes the laboratory bench, the centre of laboratory bench is provided with the recess, the top fixed mounting of recess has analytic device, analytic device is including solid fixed ring, gu fixed ring's axle center department fixedly connected with bearing, the axle center department fixedly connected with column spinner of bearing, the analytic dish of top fixedly connected with of column spinner, the top of analytic dish is provided with puts into the check, the centre at analytic dish top is provided with the collecting vat, put into the check with fixedly connected with infiltration groove between the collecting vat.

Preferably, a control cavity is formed in the bottom of the experiment table, a motor is fixedly mounted in the middle of the bottom of the control cavity, and a rotating gear is fixedly connected to one end of an output shaft of the motor.

The left and right sides meshing of rotary gear has driven gear, driven gear's axle center department fixed mounting has the rotary rod, and extends to the top in control chamber.

Preferably, the top of the experiment table is communicated with a dropping pipe.

Preferably, the top of the dropping pipe is fixedly connected with a storage bottle.

Preferably, an injection hole is fixedly installed at the top of the storage bottle, and a control valve is fixedly installed at one side of the storage bottle.

Compared with the prior art, the beneficial effects of the utility model are that: the utility model discloses the setting is passed through analytic device's analytic dish positive and negative rotation oscillation and high-speed rotation move liquid, has removed traditional reaction unit from and has relied on long basin oscillation reaction liquid, the numerous and complex operation that the straw moved liquid, can change the rotational speed through the motor and control slow oscillation and quick centrifugal step, and the storage bottle can pour into reagent in advance, through the weight that control flap control was poured into, and when the motor rotated, can cooperate to instil into the pipe and instil into, instil into at every turn and mutually support with motor slew time, do not need the manual work to go to the fractional numerical control system and pour into, the utility model discloses simple structure simple operation has realized centrifugal dehydration and has moved the mesh of liquid, but wide application in nucleic acid molecule hybridization experiment.

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the internal structure of the bottom of the experiment table of the present invention;

fig. 3 is a schematic structural diagram of the resolver of the present invention.

In the figure: 1. a laboratory bench; 2. a resolving device; 201. a fixing ring; 202. a bearing; 203. a spin column; 204. analyzing the disc; 205. collecting tank; 206. placing the cells into a grid; 207. infiltrating into a groove; 3. a control valve; 4. A storage bottle; 5. a dropping pipe; 6. an injection hole; 7. a control chamber; 8. a driven gear; 9. rotating the rod; 10. A rotating gear; 11. a motor; 12. and (4) a groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-3, the present invention provides an embodiment:

the utility model provides a nucleic acid hybridization experimental apparatus, includes laboratory bench 1, the centre of laboratory bench 1 is provided with recess 12, the top fixed mounting of recess 12 has analytical equipment 2, analytical equipment 2 is including solid fixed ring 201, gu fixed ring 201's axle center department fixedly connected with bearing 202, bearing 202's axle center department fixedly connected with column spinner 203, column spinner 203's top fixedly connected with analyzes dish 204, the top of analyzing dish 204 is provided with puts into check 206, the centre at analysis dish 204 top is provided with collecting vat 205, put into check 206 with fixedly connected with infiltration groove 207 between the collecting vat 205.

The bearing 202 is internally connected with an output shaft of the motor 11, the output shaft of the motor 11 rotates to drive the bearing 202 to rotate, and the outer side of the bearing 202 is fixed with the fixing ring 201 and cannot rotate.

The end of the infiltration tank 207 connected with the collection tank 205 is higher, so that the hybridization solution does not flow back but can be thrown out by centrifugal force.

In this embodiment, a control chamber 7 is provided in the bottom of the experiment table 1, a motor 11 is fixedly installed in the middle of the bottom of the control chamber 7, and a rotating gear 10 is fixedly connected to one end of an output shaft of the motor 11.

The motor 11 is connected with an external power supply, and the rotating speed of the motor 11 can be changed through the controller.

In this embodiment, the left and right sides of the rotating gear 10 are engaged with driven gears 8, and a rotating rod 9 is fixedly mounted at the axis of the driven gears 8 and extends to the top of the control chamber 7.

The top of the experiment table 1 is communicated with a dropping pipe 5.

Two dripping pipes 5 are provided, and the hybridization solution can be injected into the two insertion cells 206 at the same time.

The top of the dropping pipe 5 is fixedly connected with a storage bottle 4.

The storage bottle 4 is provided with two.

The top of the storage bottle 4 is fixedly provided with an injection hole 6, and one side of the storage bottle 4 is fixedly provided with a control valve 3.

The control valve 3 can control the amount of the hybridization solution to be injected.

The working principle is as follows: in operation, the hybridization liquid is injected into the storage bottle 4 through the injection hole 6 in the storage bottle 4, and then the hybridization film strip is added into the insertion cell 206 in the analysis plate 204 of the analysis device 2, then injecting a certain proportion of hybridization solution, the motor 11 drives the rotary gear 10 to rotate, the rotary gear 10 drives the left and right driven gears 8 to drive the two rotary rods 9 to rotate and drive the analysis device 2 to rotate, the rotating angle is the same with the angle between the plurality of insertion cells 206, after the hybridization solution is injected into each insertion cell 206, the control valve 3 is closed to stop injecting the hybridization solution, then the motor 11 controls the accelerating speed to drive the analysis device to vibrate for a certain time to make the hybridization membrane strip and the hybridization solution fully mixed and reacted, and controlling the motor 11 to accelerate the rotating speed again to form centrifugal force, and throwing the hybrid liquid mixed in the hybrid membrane strips placed in the grids 206 into the collecting tank through the infiltration tank 207 under the action of the centrifugal force.

And after the reaction is finished, immersing the membrane strip into the termination solution, taking out the membrane strip after the reaction is finished and the color development and shaping are carried out, scanning the membrane strip by using a scanner, and automatically archiving the result.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.

Claims

1. A nucleic acid hybridization experiment device comprises an experiment table, and is characterized in that: the middle of laboratory bench is provided with the recess, the top fixed mounting of recess has analytic device, analytic device is including solid fixed ring, gu fixed ring's axle center department fixedly connected with bearing, the axle center department fixedly connected with column spinner of bearing, the analytic dish of top fixedly connected with of column spinner, the top of analytic dish is provided with puts into the check, the centre at analytic dish top is provided with the collecting vat, put into the check with fixedly connected with infiltration groove between the collecting vat.

2. The nucleic acid hybridization assay device according to claim 1, wherein: the laboratory bench bottom is inside to be seted up the control chamber, the centre fixed mounting of control chamber bottom has the motor, the one end fixedly connected with rotary gear of motor output shaft.

3. The nucleic acid hybridization assay device according to claim 2, wherein: the left and right sides meshing of rotary gear has driven gear, driven gear's axle center department fixed mounting has the rotary rod, and extends to the top in control chamber.

4. The nucleic acid hybridization assay device according to claim 1, wherein: the top of the experiment table is communicated with a dropping pipe.

5. The nucleic acid hybridization assay device according to claim 4, wherein: the top of the dropping pipe is fixedly connected with a storage bottle.

6. The nucleic acid hybridization assay device according to claim 5, wherein: the injection hole is fixedly installed at the top of the storage bottle, and the control valve is fixedly installed on one side of the storage bottle.