CN215250811U

CN215250811U - Proteomics sample exosome separation device

Info

Publication number: CN215250811U
Application number: CN202121691050.0U
Authority: CN
Inventors: 吴泽明; 尹沛源
Original assignee: Yunpukang Dalian Biotechnology Co ltd
Current assignee: Yunpukang Dalian Biotechnology Co ltd
Priority date: 2021-07-23
Filing date: 2021-07-23
Publication date: 2021-12-21
Anticipated expiration: 2031-07-23

Abstract

The utility model discloses a proteomics sample exosome separator, the on-line screen storage device comprises a base, the base top is equipped with the storage bucket, the inside of storing the bucket is equipped with the filter vat, the lower surface central point of base puts and installs the frame, the internally mounted of frame has the motor, the output shaft top of motor runs through the first cardboard of base and fixedly connected with, the lower surface of storing the bucket is seted up flutedly. The utility model discloses in, through the filter vat that sets up, greatly increased the filtration separation area to increase filter speed, cooperate through first cardboard and first fixed block to be convenient for dismantle the storage bucket off, cooperate through second cardboard and second fixed block to be convenient for dismantle the filter vat from the storage bucket in, thereby realize the split of each subassembly of separator, the washing of each subassembly in the later stage of not only being convenient for, the processing of the material after also being convenient for filter moreover.

Description

Proteomics sample exosome separation device

Technical Field

The utility model relates to a separator technical field especially relates to a proteomics sample exosome separator.

Background

The exosome refers to a disc-shaped vesicle with the diameter of 40-100nm, various cells can secrete the exosome under normal and pathological states, and the exosome is mainly derived from a multivesicular body formed by invagination of intracellular lysosome particles and is released into extracellular matrix after fusion of the outer membrane of the multivesicular body and cell membrane;

now relate to a proteomics sample exosome separator, the in-process that has now the technique is separating exosome, because of filter membrane filtration area is less, and filtration efficiency is low, has reduced the efficiency of experiment, and the extraction cost is higher, and current exosome separator structure is fixed, is not convenient for dismantle and wash to be not convenient for handle the material after the separation, greatly increased user's work burden.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects existing in the prior art and providing a proteomics sample exosome separation device.

In order to achieve the above purpose, the utility model adopts the following technical scheme: a proteomics sample exosome separating device comprises a base, wherein a storage barrel is arranged above the base, a filter barrel is arranged inside the storage barrel, a rack is arranged at the center of the lower surface of the base, a motor is arranged inside the rack, the top end of an output shaft of the motor penetrates through the base and is fixedly connected with a first clamping plate, a groove is formed in the lower surface of the storage barrel, the top of the groove penetrates through and is rotatably connected with a rotating shaft, a first fixing block is fixedly connected to the bottom end of the rotating shaft, a second clamping plate is fixedly connected to the top end of the rotating shaft, a second fixing block is fixedly connected to the lower surface of the filter barrel, a first top cover is clamped at the top end of the storage barrel, a support tube is fixedly connected to the center of the lower surface of the first top cover, an ejector rod penetrates through and is slidably connected to the inside of the support tube, and the bottom end of the ejector rod is rotatably connected with a third clamping plate, the top end threaded connection of filter vat has the second top cap, the last fixed surface of second top cap is connected with the third fixed block.

Furthermore, the upper surface of the base is symmetrically and fixedly connected with positioning rods, and the lower surface of the storage barrel is provided with positioning holes corresponding to the positioning rods.

Furthermore, a first clamping groove corresponding to the first clamping plate is formed in the lower surface of the first fixing block, and the top end of the first clamping plate is embedded into the first clamping groove.

Furthermore, a second clamping groove corresponding to the second clamping plate is formed in the lower surface of the second fixing block, and the top end of the second clamping plate is embedded into the second clamping groove.

Furthermore, a third clamping groove corresponding to the third clamping plate is formed in the upper surface of the third fixing block, and the bottom end of the third clamping plate is embedded into the third clamping groove.

Furthermore, the top of the interior of the supporting tube is fixedly connected with a spring, and the bottom end of the spring is fixedly connected with the top end of the ejector rod.

Furthermore, the lower surface of the base is symmetrically and fixedly provided with a support frame.

Further, the upper surface of base and the position symmetry fixedly connected with backup pad that is located storage bucket both sides, the surface of backup pad is run through has the screw rod through threaded connection, the screw rod is close to the one end rotation that stores the bucket and is connected with splint.

Furthermore, one end of the screw rod, which is far away from the storage barrel, is fixedly connected with a turntable.

Furthermore, a rubber pad is arranged on one side of the clamping plate close to the storage barrel.

The utility model has the advantages that:

the utility model discloses when using, this proteomics sample exosome separator, through the filter vat that sets up, greatly increased filtration separation area, thereby increase filter speed, cooperate through first cardboard and first fixed block to be convenient for to dismantle storage barrel down, cooperate through second cardboard and second fixed block to be convenient for to dismantle filter vat from storage barrel in, thereby realize the split of each subassembly of separator, the washing of each subassembly in the later stage of not only being convenient for, and also be convenient for the processing of the material after filtering.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic view of the internal structure of the storage barrel of the present invention;

fig. 3 is an enlarged view of a in fig. 1 according to the present invention.

Illustration of the drawings:

1. a base; 2. a storage barrel; 3. a filter vat; 4. a frame; 5. a motor; 6. a first clamping plate; 7. a groove; 8. a rotating shaft; 9. a first fixed block; 10. a second clamping plate; 11. a second fixed block; 12. a first top cover; 13. supporting a tube; 14. a top rod; 15. a third clamping plate; 16. a second top cover; 17. a third fixed block; 18. positioning a rod; 19. positioning holes; 20. a first card slot; 21. a second card slot; 22. a third card slot; 23. a spring; 24. a support frame; 25. a support plate; 26. a screw; 27. a splint; 28. a turntable.

Detailed Description

Fig. 1 to 3 show, which relate to a proteomics sample exosome separation device, comprising a base 1, a storage barrel 2 is arranged above the base 1, a filter barrel 3 is arranged inside the storage barrel 2, a frame 4 is arranged at the center of the lower surface of the base 1, a motor 5 is arranged inside the frame 4, the top end of the output shaft of the motor 5 penetrates through the base 1 and is fixedly connected with a first clamping plate 6, a groove 7 is arranged on the lower surface of the storage barrel 2, the top of the groove 7 penetrates through and is rotatably connected with a rotating shaft 8, the bottom end of the rotating shaft 8 is fixedly connected with a first fixing block 9, the top end of the rotating shaft 8 is fixedly connected with a second clamping plate 10, the lower surface of the filter barrel 3 is fixedly connected with a second fixing block 11, the top end of the storage barrel 2 is clamped with a first top cover 12, the center of the lower surface of the first top cover 12 is fixedly connected with a support tube 13, and the inside of the support tube 13 penetrates through and is slidably connected with a push rod 14, the bottom end of the ejector rod 14 is rotatably connected with a third clamping plate 15, the top end of the filter barrel 3 is in threaded connection with a second top cover 16, and the upper surface of the second top cover 16 is fixedly connected with a third fixing block 17.

When the proteome sample exosome separating device is used, a storage barrel 2 is placed on a base 1, position limitation is carried out through a positioning rod 18 and a positioning hole 19, a first clamping plate 6 is clamped into a first clamping groove 20 on a first fixing block 9, then rotary tables 28 on two sides are rotated, clamping plates 27 are driven through a screw 26 to fix the storage barrel 2, then a filter barrel 3 is placed into the storage barrel 2, a second clamping plate 10 is clamped into a second clamping groove 21 on a second fixing block 11, then a top cover is clamped, a third clamping plate 15 is clamped into a third clamping groove 22 on a third fixing block 17, then a motor 5 is started, the motor 5 rotates to drive a rotating shaft 8 to rotate through the first clamping plate 6 and the first fixing plate, and further, the filter barrel 3 is driven to rotate through the second clamping plate 10 and the second fixing block 11, so that centrifugal separation operation is realized, through the arranged filter barrel 3, can greatly increased filter area to greatly increased separating speed, and then improve work efficiency, when needs dismantle the washing to the device, only need open the splint 27 and the first top cap 12 of both sides, and then alright get off with dismantling storage bucket 2 and filter vat 3 respectively.

In a further scheme, the upper surface of the base 1 is symmetrically and fixedly connected with positioning rods 18, the lower surface of the storage barrel 2 is provided with positioning holes 19 corresponding to the positioning rods 18, and the storage barrel 2 is conveniently subjected to position limitation through the matching of the positioning rods 18 and the positioning holes 19.

In a further scheme, a first clamping groove 20 corresponding to the first clamping plate 6 is formed in the lower surface of the first fixing block 9, the top end of the first clamping plate 6 is embedded into the first clamping groove 20, and the first clamping plate 6 can be driven to rotate when rotating through the arranged first clamping groove 20, so that the rotating shaft 8 is driven to rotate.

In a further scheme, a second clamping groove 21 corresponding to the second clamping plate 10 is formed in the lower surface of the second fixing block 11, the top end of the second clamping plate 10 is embedded into the second clamping groove 21, and the second clamping plate 10 can be driven to rotate by the aid of the second clamping groove 21, so that the second fixing block 11 is driven to rotate, and the filter barrel 3 is driven to rotate.

In a further scheme, a third clamping groove 22 corresponding to the third clamping plate 15 is formed in the upper surface of the third fixing block 17, the bottom end of the third clamping plate 15 is embedded into the third clamping groove 22, the third clamping plate 15 can be connected with the third fixing block 17 through the third clamping groove 22, and therefore the top of the filter barrel 3 can be supported conveniently.

In a further scheme, a spring 23 is fixedly connected to the top of the interior of the support tube 13, the bottom end of the spring 23 is fixedly connected to the top end of the ejector rod 14, and the third clamping plate 15 can be stably clamped in the third clamping groove 22 through the arranged spring 23, so that the stability of the filter vat 3 is improved.

In a further scheme, the lower surface of the base 1 is symmetrically and fixedly provided with a support frame 24, and the support frame 24 is convenient for placing the device.

In the further scheme, the upper surface of base 1 just is located the position symmetry fixedly connected with backup pad 25 of storing 2 both sides of bucket, the surface of backup pad 25 runs through there is screw rod 26 through threaded connection, screw rod 26 is close to the one end rotation of storing bucket 2 and is connected with splint 27, and screw rod 26 rotates and drives splint 27 and remove to can fix storing bucket 2, appear rocking when avoiding using.

In a further embodiment, a rotating disc 28 is fixedly connected to an end of the screw 26 away from the storage barrel 2, and the rotating disc 28 is for facilitating rotation of the screw 26.

In a further embodiment, a rubber pad is disposed on a side of the clamping plate 27 close to the storage barrel 2, and the rubber pad is used for increasing the friction force between the clamping plate 27 and the filter barrel 3.

The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.

Claims

1. A proteomics sample exosome separation device, comprising a base (1), characterized in that: a storage barrel (2) is arranged above the base (1), a filter barrel (3) is arranged inside the storage barrel (2), a rack (4) is arranged at the center of the lower surface of the base (1), a motor (5) is arranged inside the rack (4), the top end of an output shaft of the motor (5) penetrates through the base (1) and is fixedly connected with a first clamping plate (6), a groove (7) is formed in the lower surface of the storage barrel (2), the top of the groove (7) is rotatably connected with a rotating shaft (8) in a penetrating manner, a first fixing block (9) is fixedly connected with the bottom end of the rotating shaft (8), a second clamping plate (10) is fixedly connected with the top end of the rotating shaft (8), a second fixing block (11) is fixedly connected with the lower surface of the filter barrel (3), a first top cover (12) is clamped at the top end of the storage barrel (2), a support pipe (13) is fixedly connected with the center of the lower surface of the first top cover (12), the inside of stay tube (13) runs through sliding connection and is connected with ejector pin (14), the bottom of ejector pin (14) is rotated and is connected with third cardboard (15), the top threaded connection of filter vat (3) has second top cap (16), the last fixed surface of second top cap (16) is connected with third fixed block (17).

2. The proteomic sample exosome separation device according to claim 1, wherein: the upper surface of the base (1) is symmetrically and fixedly connected with positioning rods (18), and the lower surface of the storage barrel (2) is provided with positioning holes (19) corresponding to the positioning rods (18).

3. The proteomic sample exosome separation device according to claim 1, wherein: the lower surface of the first fixing block (9) is provided with a first clamping groove (20) corresponding to the first clamping plate (6), and the top end of the first clamping plate (6) is embedded into the first clamping groove (20).

4. The proteomic sample exosome separation device according to claim 1, wherein: the lower surface of the second fixing block (11) is provided with a second clamping groove (21) corresponding to the second clamping plate (10), and the top end of the second clamping plate (10) is embedded into the second clamping groove (21).

5. The proteomic sample exosome separation device according to claim 1, wherein: the upper surface of the third fixing block (17) is provided with a third clamping groove (22) corresponding to the third clamping plate (15), and the bottom end of the third clamping plate (15) is embedded into the third clamping groove (22).

6. The proteomic sample exosome separation device according to claim 1, wherein: the top of the interior of the supporting tube (13) is fixedly connected with a spring (23), and the bottom end of the spring (23) is fixedly connected with the top end of the ejector rod (14).

7. The proteomic sample exosome separation device according to claim 1, wherein: the lower surface of the base (1) is symmetrically and fixedly provided with a support frame (24).

8. The proteomic sample exosome separation device according to claim 1, wherein: the upper surface of base (1) and the position symmetry fixedly connected with backup pad (25) that is located storage bucket (2) both sides, the surface of backup pad (25) runs through screw rod (26) through threaded connection, screw rod (26) are close to the one end rotation that stores bucket (2) and are connected with splint (27).

9. The proteomic sample exosome separation device according to claim 8, wherein: one end of the screw rod (26) far away from the storage barrel (2) is fixedly connected with a rotary disc (28).

10. The proteomic sample exosome separation device according to claim 8, wherein: a rubber pad is arranged on one side of the clamping plate (27) close to the storage barrel (2).