CN216473191U - Tissue dissociation device - Google Patents

Abstract

The utility model discloses a tissue dissociation device, and belongs to the technical field of tissue dissociation. This tissue dissociation device includes the sample bottle and grinds the filtration, grinds the filtration including grinding piece and filter screen, and the grinding end and the filter screen that grind the piece can be through the sample bottle put the thing mouth get into the sample bottle put the thing space in to will put the thing space and divide into the tissue and hold the district and the cell suspension holds the district, grind the end have the butt on the filter screen the sealed position and with the filter position of filter screen separation. The grinding end in the sealing position can rotatably grind biological tissues in the tissue accommodating area, the cell suspension accommodating area is in a sealing state at the moment, when the grinding is finished and the grinding end moves to the filtering position, the cell suspension accommodating area is in a negative pressure state, and cell suspension formed by grinding the biological tissues can be filtered through the filter screen and enters the cell suspension accommodating area to be temporarily stored. The tissue dissociation device has high integration level and good filtering effect, and can form high-quality cell suspension.

Description

Tissue dissociation device

Technical Field

The utility model relates to the technical field of tissue dissociation, in particular to a tissue dissociation device.

Background

The tissue dissociation device is equipment for dissociating biological tissues by using a tissue dissociation technology to obtain high-activity and high-quality cell suspension. To obtain a cell suspension, the tissue dissociation device first needs to destroy the extracellular matrix to release cells from the extracellular matrix, and then needs to destroy the cell-to-cell connection to obtain the cell suspension.

There are many methods for tissue dissociation, such as enzymolysis, electrolysis, or grinding. Since the grinding can sufficiently shear the tissue, the dissociation effect is good, and the grinding is widely used. The existing device using grinding as a tissue dissociation method cannot directly filter dissociated cell suspension, and only can transfer the cell suspension into an external filtering device for filtering after grinding is finished, so that the operation is complicated, and the activity and the quality of the cell suspension can be influenced in the transfer process.

Therefore, how to provide a tissue dissociation device integrating grinding and filtering is a technical problem which needs to be solved urgently.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a tissue dissociation device which integrates grinding and filtering, and has higher integration degree and good filtering effect.

In order to achieve the purpose, the utility model adopts the following technical scheme:

a tissue dissociation device, comprising: a sample bottle having a storage space and a storage port; the grinding and filtering structure comprises a grinding part and a filter screen, the filter screen is movably connected with a grinding end of the grinding part, the grinding end and the filter screen can enter the object placing space through the object placing opening and divide the object placing space into a cell suspension accommodating area positioned on one side of the filter screen and a tissue accommodating area positioned on one side of the grinding end, and the grinding end is provided with a sealing position which is abutted against the filter screen to seal the cell suspension accommodating area and a filtering position which is separated from the filter screen; the grinding end is configured to be rotatable about a first axis a within the sample vial when in the sealed position to grind biological tissue located within the tissue containment region; and the grinding end is configured to be capable of performing linear motion along the first axis a in a direction away from the filter screen and move from the sealing position to the filtering position, so that cell suspension formed by grinding the biological tissue is filtered through the filter screen and enters the cell suspension containing area under the action of negative pressure.

Preferably, the grinding end is configured to be capable of moving linearly along the first axis a in a direction away from the filter screen and moving from the filtering position to a limiting position, and the grinding end at the limiting position can be in limit abutment with the filter screen, so that the grinding end can drive the filter screen to move synchronously along the first axis a.

Preferably, the grinding end comprises a grinding rotor, a grinding stator and a fixing piece, a rotating shaft hole penetrates through the grinding stator along the first axis a, and a grinding surface is arranged on the grinding stator; the grinding rotor comprises a rotor shaft and a grinding blade, one end of the grinding blade is connected to the middle of the rotor shaft, the first end of the rotor shaft is used for connecting a driving part, the second end of the rotor shaft movably penetrates through the rotating shaft hole so that the other end of the grinding blade is in contact with the grinding surface of the grinding stator, and the grinding blade can grind the biological tissue on the grinding surface when the grinding rotor rotates; the middle part of filter screen is provided with the through-hole, the link of mounting can pass the through-hole with the second end of rotor shaft is connected, just be provided with on the mounting and can pass the through-hole and with grind the stator and carry out spacing first limit structure, the spacing end of mounting is provided with second limit structure, second limit structure can be spacing the filter screen is kept away from grind one side of stator.

Preferably, the fixing part is a stepped shaft, the outer diameter of the stepped shaft is smaller than the size of the through hole, the stepped shaft comprises a small-diameter shaft and a large-diameter shaft which are coaxially connected, one end, far away from the large-diameter shaft, of the small-diameter shaft is connected with the second end of the rotor shaft, and the first limiting structure is formed between the small-diameter shaft and the large-diameter shaft to form a first limiting surface; the pivot hole is the shoulder hole, and it is including the aperture hole and the big aperture hole that connect gradually, big aperture hole is close to the filter screen sets up, aperture hole with form the spacing face of second between the big aperture hole, first spacing face can with the spacing butt of the spacing face of second.

Preferably, the second limiting structure is a limiting boss, the limiting boss is arranged at one end, away from the small-diameter shaft, of the large-diameter shaft, and the size of the limiting boss is larger than that of the through hole.

Preferably, the grinding surface is a conical surface, and the other end of the grinding blade is obliquely provided with a grinding blade edge which can move on the conical surface to cut the biological tissue.

Preferably, the grinding surface is provided with a plurality of grinding grooves which rotate around the first axis a.

Preferably, the inner wall surface of the sample bottle is provided with a positioning step, and the filter screen can be arranged on the positioning step.

Preferably, a third limiting structure is arranged between the grinding stator and the sample bottle to limit the grinding stator to rotate around the first axis a.

Preferably, the third limiting structure comprises a limiting block arranged on the inner wall surface of the sample bottle and a limiting groove arranged on the outer wall surface of the grinding stator, and the limiting block can be inserted into the limiting groove.

The utility model has the beneficial effects that:

the utility model provides a tissue dissociation device, which comprises a sample bottle and a grinding and filtering structure, wherein the grinding and filtering structure comprises a grinding piece and a filter screen movably connected to the grinding end of the grinding piece, the grinding end and the filter screen can enter an object placing space of the sample bottle through an object placing opening of the sample bottle and divide the object placing space into a cell suspension containing area and a tissue containing area, and the grinding end is provided with a sealing position abutting against the filter screen and a filtering position separated from the filter screen. When grinding the end and being in sealed position, grind the end and can rotate the biological tissue that grinds to be located the tissue holding area, cell suspension holding area is in encapsulated situation this moment, when grinding the completion and grind the end and remove to the filtration position, is in the negative pressure state in the cell suspension holding area, and under atmospheric pressure's effect, the cell suspension that grinds biological tissue and form this moment can filter through the filter screen to it keeps in entering cell suspension holding area. This tissue dissociation device will grind piece and filter screen integration together, and the integrated level is high, is favorable to improving the production efficiency and the activity of cell suspension, and filters cell suspension under the negative pressure effect, and the filter effect is better, does benefit to the cell suspension that forms high-quality.

Drawings

Fig. 1 is a schematic structural diagram of a tissue dissociation device according to an embodiment of the present invention;

fig. 2 is a cross-sectional view of a tissue dissociation device provided in accordance with an embodiment of the present invention;

fig. 3 is an exploded view of a tissue dissociation device provided by an embodiment of the present invention;

fig. 4 is a cross-sectional view of a sample vial of a tissue dissociation device provided in an embodiment of the utility model;

FIG. 5 is a cross-sectional view of an abrasive element of a tissue dissociation device provided in accordance with an embodiment of the utility model;

fig. 6 is an exploded view of an abrasive member of a tissue dissociation device according to an embodiment of the utility model.

In the figure:

100. a sample bottle; 101. an object placing space; 102. a storage port; 103. positioning a step; 104. a limiting block;

200. grinding the filter structure; 201. a filter screen; 202. grinding the rotor; 2021. a rotor shaft; 2022. grinding the blade; 203. grinding the stator; 2031. grinding the surface; 2032. a grinding groove; 2033. a rotating shaft hole; 2034. a second limiting surface; 204. a fixing member; 2041. a first limiting surface; 2042. a small diameter shaft; 2043. a large diameter shaft; 2044. a limiting boss; 205. a bottle cap;

300. a tissue receiving area;

400. a cell suspension containment zone.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Wherein the terms "first position" and "second position" are two different positions.

In the description of the present invention, it should be noted that unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection or a removable connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The embodiment provides a tissue dissociation device, and this tissue dissociation device integrated level is higher, not only can fully grind biological tissue, and can fully filter the biological tissue after grinding to form high-quality, high-activity cell suspension. Scientific researchers can obtain more accurate detection data or test data by detecting or testing the cell suspension, so that more accurate scientific research results are obtained.

As shown in fig. 1 to 6, the tissue dissociation apparatus includes a sample bottle 100 and a grinding filter structure 200. Wherein, sample bottle 100 is inside to have storage space 101, and storage space 101 runs through sample bottle 100's one end to form and put thing mouth 102, can place grinding filtration 200 and biological tissue in sample bottle 100's storage space 101 through this storage mouth 102. In this embodiment, as shown in fig. 1 to 4, the sample bottle 100 is in the shape of a test tube and has a cylindrical shape, and the sample bottle 100 can be directly placed on a centrifuge to perform centrifugation of the finally obtained cell suspension. More specifically, the sample bottle 100 includes a straight cylinder portion and a tapered portion, the placement opening 102 is disposed at an end of the straight cylinder portion away from the tapered portion, and the inclined inner wall surface of the tapered portion facilitates the collection of the cell suspension to the bottom end of the sample bottle 100, thereby avoiding the phenomenon of wall hanging.

The abrasive filter structure 200 includes an abrasive member and a filter mesh 201. Wherein, the grinding piece has a driving end and two ends of a grinding end. The driving end is used for connecting an external driving part, and the driving part can be a rotating motor. The grinding end is used for grinding biological tissues, the grinding end and the filter screen 201 can enter the object placing space 101 through the object placing port 102, the object placing space 101 is divided into a cell suspension containing area 400 located on one side of the filter screen 201 and a tissue containing area 300 located on one side of the grinding end, the tissue containing area 300 is used for containing the biological tissues and dissociation liquid, and the cell suspension containing area 400 is used for containing generated cell suspensions. The filter screen 201 is movably attached to the grinding end of the grinding member and has a sealing position abutting the filter screen 201 to seal the cell suspension containing zone 400 during movement of the grinding end and a filtering position separated from the filter screen 201 to create a negative pressure within the cell suspension containing zone 400.

When the grinding end moves to the sealing position, the grinding end abuts against the filter screen 201, so that the cell suspension containing area 400 below the filter screen 201 forms a sealed space, and the biological tissue and the dissociation liquid in the tissue containing area 300 are gathered at the grinding end, so that the sealing performance of the cell suspension containing area 400 is further improved. At this time, when the grinding member is driven by the driving part, the grinding end of the grinding member can rotate around the first axis a (as shown in fig. 2) in the sample bottle 100, the rotating grinding end can grind the biological tissue in the tissue accommodating area 300, and the biological tissue is divided, ground and dissociated in the dissociation liquid under the mechanical force, so as to form a cell suspension doped with tissue fragments. After the grinding is accomplished, grind the end and can follow first axis a under the exogenic action and carry out rectilinear motion to the direction of keeping away from filter screen 201, thereby make and grind the end and remove to the filter position by sealed position, at this moment, grind the end and part with filter screen 201, the cell suspension that is located to grind the end below holds district 400 space grow, pressure diminishes, thereby be in the negative pressure state that is less than atmospheric pressure, the cell suspension that grinds biological tissue formation filters through filter screen 201 under the negative pressure effect, high quality after the filtration, high-activity cell suspension gets into and temporarily stores in cell suspension holds district 400.

Compare in the tissue dissociation device that only has grinding function among the prior art, the tissue dissociation device that this embodiment provided is through being in the same place grinding piece and filter screen 201 integration to not only have grinding and filterable dual function concurrently, functional strong, the integrated level is high, and can filter cell suspension in the fastest time that grinds the completion, is favorable to improving cell suspension's production efficiency and activity. And, compare in utilizing gravity to realize filtering, the cell suspension is filtered under the negative pressure effect through the filterable filtration mode of negative pressure to the tissue dissociation device that this embodiment provided, and the filter effect is better, does benefit to and forms high-quality cell suspension.

Optionally, after the cell suspension is filtered and enters the cell suspension containing region 400 for temporary storage, the grinding end and the filter screen 201 need to be taken out of the sample bottle 100, so as to move the sample bottle 100 containing the cell suspension to other devices for the next processing procedure, and perform subsequent processing on the cell suspension, in order to improve the transfer efficiency and reduce the difficulty in moving out the filter screen 201, the grinding end of the grinding member provided by the embodiment can continue to perform linear motion in the direction away from the filter screen 201 along the first axis a under the driving of external force, and thus the grinding end is moved to the limit position from the filtering position. When grinding the end and being in limit position, grind the end and can with the spacing butt of filter screen 201, continue to follow first axis a this moment and remove the grinding end to the direction of keeping away from filter screen 201, grind the end and can drive filter screen 201 and carry out synchronous motion along first axis a to realize grinding piece and filter screen 201 and follow the synchronous shifting out in sample bottle 100.

Specifically, as shown with continued reference to fig. 5 and 6, the grinding tip includes a grinding rotor 202, a grinding stator 203, and a fixture 204. Wherein, after putting the grinding end in sample bottle 100, grinding stator 203 is fixed to be set up in sample bottle 100, and can not take place to rotate around first axis a, and the fixed of here can be realized through location structure, for example constant head tank and locating piece, and constant head tank and locating piece one of them sets up on the internal wall of sample bottle 100, and another sets up on grinding stator 203. The grinding stator 203 is provided with a spindle hole 2033 extending in the first axis a direction, and the grinding stator 203 is provided with a grinding surface 2031. The ground surface 2031 is the bottom surface of the tissue receiving area 300, and the biological tissue entering the tissue receiving area 300 is located on the ground surface 2031 and is cut and ground on the ground surface 2031 by the grinding rotor 202.

Optionally, the grinding stator 203 is in the shape of a frustum, which includes a conical surface, which is the grinding surface 2031. The grinding surface 2031 is a conical surface, which increases the area of the grinding surface 2031, so that more biological tissues can be loaded on the grinding surface 2031, thereby improving the grinding efficiency. Further optionally, a plurality of grinding grooves 2032 are provided on the grinding surface 2031, the number of the grinding grooves 2032 is plural, and the plurality of grinding grooves 2032 rotate around the first axis a and are in a spiral shape. The arrangement of the grinding groove 2032 can further improve the grinding efficiency and the grinding effect. According to the demand, can set up the multilayer on grinding face 2031 and grind groove 2032, the grinding groove 2032 of each layer all includes a plurality of grinding grooves 2032, and a plurality of grinding grooves 2032 of each layer all encircle first axis a rotation along a direction, and two-layer grinding groove 2032 on the adjacent setting can the dislocation set, also can connect gradually, and the rotation can be the same, also can be different.

Further, in order to stably place the filter 201 in the sample bottle 100, as shown in fig. 4, a positioning step 103 is provided on the inner wall surface of the sample bottle 100, and the filter 201 can be placed on the positioning step 103. Since the grinding stator 203 is disposed above the filter screen 201, in order to stably position the grinding stator 203 at the sealing position, an annular convex edge may be protruded upward along the circumferential direction of the filter screen 201, and the bottom of the positioning step 103 is set to be a stepped portion, an annular horizontal plane on the stepped portion is stopped on the top surface of the annular convex edge, and a portion of the stepped portion located below the annular horizontal plane is disposed inside the annular convex edge.

Further, in order to avoid that the grinding stator 203 rotates with the grinding rotor 202 during grinding, thereby causing deterioration of grinding effect, a third limiting structure is provided between the grinding stator 203 and the sample bottle 100 to limit the grinding stator 203 from rotating about the first axis a. Specifically, the third limiting structure includes a limiting block 104 and a limiting groove, the limiting block 104 is disposed on the inner wall surface of the sample bottle 100, and more specifically, may be disposed on the positioning step 103, the limiting groove is disposed on the outer wall surface of the grinding stator 203, and the limiting block 104 can be inserted into the limiting groove. Of course, in other embodiments, the positions of the stopper 104 and the stopper groove may be interchanged, that is, the stopper groove is disposed on the inner wall surface of the sample bottle 100, and the stopper 104 is disposed on the outer wall surface of the grinding stator 203. Of course, in other embodiments, the third limiting structure may also be disposed between the grinding stator 203 and the filter screen 201, which is not described in detail herein.

With continued reference to fig. 4 and 5, the grinding rotor 202 includes a rotor shaft 2021 and grinding blades 2022. The rotor shaft 2021 is a cylindrical structure, and includes a first end and a second end, where the first end of the rotor shaft 2021 is used to connect a driving component. And a cap 205 sealing the sample vial 100 may also be provided on the first end of the rotor shaft 2021, the cap 205 being capable of sealing the tissue receiving region 300 to prevent foreign materials from entering the tissue receiving region 300 during tissue dissociation. The second end of the rotor shaft 2021 is movably disposed through the spindle bore 2033, where the second end may be disposed within the spindle bore 2033 or may protrude below the grinding stator 203 through the spindle bore 2033. The grinding blade 2022 is a curved blade, one end of the grinding blade 2022 is connected to the middle part of the rotor shaft 2021, and the other end is spirally extended downward. When the second end of the rotor shaft 2021 is inserted into the rotating shaft hole 2033, the other end of the grinding blade 2022 contacts the grinding surface 2031 of the grinding stator 203. When the rotor shaft 2021 is driven by the driving member and rotates around the first axis a, the other end of the grinding blade 2022 can move on the grinding surface 2031 of the grinding stator 203 and cut and grind the biological tissue on the grinding surface 2031, so that the cut biological tissue is uniformly mixed with the dissociation liquid and dissociated by the dissociation liquid.

Alternatively, the rotor shaft 2021 may be a cylindrical shaft or a stepped shaft, in this embodiment, a stepped shaft is optionally used to facilitate positioning and mounting of the grinding blade 2022. Specifically, the rotor shaft 2021 includes a first shaft having a larger diameter and a second shaft having a smaller diameter, and the grinding blade 2022 has one end connected to a joint of the first shaft and the second shaft and the other end spirally extending to a position flush with a second end of the rotor shaft 2021. In order to improve the grinding efficiency, the grinding blades 2022 may be provided in two or more numbers, and the plurality of grinding blades 2022 are uniformly distributed around the circumference of the rotor shaft 2021 and have the same rotation direction. In order to increase the area of the grinding blade 2022 and the grinding surface 2031, the other end of the grinding blade 2022 is provided with a grinding blade obliquely, the grinding blade is arranged along the arc of the grinding surface 2031, and the grinding blade can move on the conical surface to cut the biological tissue.

Further optionally, one end of the rotor shaft 2021, which is away from the grinding stator 203, is a driving end of the grinding member, a connector connected to the driving part is disposed on the end, and the bottle cap 205 is detachably sleeved outside the connector. The driving unit is detachably connected to the rotor shaft 2021 via the connection head, and thereby the grinding rotor 202 is driven to rotate relative to the grinding stator 203, thereby grinding the biological tissue.

With continued reference to fig. 4 and 5, the fixing element 204 serves to achieve a relative fixing of the grinding rotor 202 and the grinding stator 203, in order to make it possible for the grinding end of the grinding stator 203 to be moved from the sealing position into the filtering position, and to achieve a relative spacing of the grinding element with respect to the filter screen 201, in order to make it possible for the grinding end of the grinding stator 203 to be moved from the filtering position into the spacing position and to achieve a synchronous movement of the grinding stator 203, the grinding rotor 202 and the filter screen 201.

Specifically, a through hole is formed in the middle of the filter screen 201, the fixing element 204 includes a connecting end and a limiting end, the connecting end of the fixing element 204 is smaller than the through hole, and the fixing element can penetrate through the through hole to be connected with the second end of the rotor shaft 2021. And the middle part of the fixing piece 204 is provided with a first limiting structure which can penetrate through the through hole and limit the grinding stator 203, the limiting end of the fixing piece 204 is provided with a second limiting structure, and the second limiting structure can limit one side of the filter screen 201 far away from the grinding stator 203.

More specifically, the fixing member 204 is a stepped shaft, and the outer diameter of the stepped shaft at any position is smaller than the size of the through hole, so that the stepped shaft can pass through the through hole. More specifically, as shown in fig. 6, the stepped shaft includes a small-diameter shaft 2042 and a large-diameter shaft 2043 which are coaxially connected, one end of the small-diameter shaft 2042 away from the large-diameter shaft 2043 is a connecting end, which is connected to the second end of the rotor shaft 2021, and the first limiting structure is formed between the small-diameter shaft 2042 and the large-diameter shaft 2043 to form a first limiting surface 2041. The pivot hole 2033 is the shoulder hole, and it is including the small aperture hole and the big aperture hole that connect gradually, and the big aperture hole is close to filter screen 201 and sets up, forms the spacing face 2034 of second between small aperture hole and the big aperture hole, and first spacing face 2041 can be with the spacing butt of the spacing face 2034 of second. The second limit structure is a limit boss 2044, the limit boss 2044 is arranged at one end of the large-diameter shaft 2043 far away from the small-diameter shaft 2042, and the size of the limit boss 2044 is larger than that of the through hole.

That is, the small-diameter shaft 2042 has a diameter smaller than that of the small-diameter hole, and the large-diameter shaft 2043 has a diameter smaller than that of the large-diameter hole and larger than the size of the small-diameter hole. When the grinding stator 203 is in the sealing position, the first limiting surface 2041 and the second limiting surface 2034 are disengaged, after grinding is completed, when the rotor shaft 2021 of the grinding rotor 202 moves upward under the action of external force, the small-diameter shaft 2042 gradually penetrates into the small-aperture hole, the large-diameter shaft 2043 gradually penetrates into the large-aperture hole, the first limiting surface 2041 and the second limiting surface 2034 gradually approach, after the first limiting surface 2041 and the second limiting surface 2034 are contacted, the relative position between the fixing member 204 and the grinding stator 203 is kept fixed, at this time, if the rotor shaft 2021 of the grinding rotor 202 continues to move upward under the action of external force, the grinding stator 203 can move upward synchronously along with the grinding rotor 202. In the process of synchronously moving the grinding stator 203, the grinding rotor 202 and the fixing piece 204 upwards, the limiting boss 2044 located below the filter screen 201 is gradually close to the filter screen 201, and because the size of the limiting boss 2044 is larger than that of the through hole in the filter screen 201, the limiting boss 2044 cannot pass through the through hole, after the limiting boss 2044 abuts against the filter screen 201, if the grinding rotor 202 continues to move upwards under the action of external force, the filter screen 201 can synchronously move upwards along with the grinding rotor 202, the grinding stator 203 and the fixing piece 204 until the filter screen 201, the grinding rotor 202, the grinding stator 203 and the fixing piece 204 move out of the sample bottle 100.

Optionally, the limiting boss 2044 may be an annular boss arranged around the circumference of the large-diameter shaft 2043, or may be a plurality of arc-shaped blocks distributed around the circumference of the large-diameter shaft 2043. Of course, the fixing member 204 may also include a cylindrical shaft and an annular boss disposed in the middle of the cylindrical shaft, besides being a stepped shaft, the diameter of the annular boss is smaller than the diameter of the through hole, smaller than the size of the large-diameter hole, and larger than the size of the small-diameter hole.

Compared with the conventional tissue dissociation device, the tissue dissociation device provided by the embodiment has the following advantages:

1. can realize grinding in the dissociation liquid, grind more fully.

2. Can be stirred in the incubation process, and the full contact of the biological tissue and the dissociation liquid is realized.

3. Can directly filter after grinding fully, and adopt the negative pressure mode to filter, the filter effect is better, does benefit to and obtains high-quality, high active cell suspension.

4. The sample bottle 100 can be directly placed in the centrifuge, and the inconvenience and various risks in the sample transfer process are reduced.

It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims (10)

1. A tissue dissociation device, comprising:

a sample bottle (100), wherein the sample bottle (100) is provided with a storage space (101) and a storage opening (102);

the grinding and filtering structure (200) comprises a grinding piece and a filtering net (201), the filtering net (201) is movably connected to a grinding end of the grinding piece, the grinding end and the filtering net (201) can enter the object placing space (101) through the object placing opening (102), the object placing space (101) is divided into a cell suspension containing area (400) located on one side of the filtering net (201) and a tissue containing area (300) located on one side of the grinding end, and the grinding end is provided with a sealing position which is abutted against the filtering net (201) to seal the cell suspension containing area (400) and a filtering position which is separated from the filtering net (201);

the grinding end is configured to be rotatable within the sample vial (100) about a first axis a when in the sealed position to grind biological tissue located within the tissue receiving region (300); and the grinding end is configured to be capable of performing linear motion along the first axis a in a direction away from the filter screen (201) and move from the sealing position to the filtering position, so that the cell suspension formed by grinding the biological tissue is filtered through the filter screen (201) under the action of negative pressure and enters the cell suspension containing area (400).

2. The tissue dissociation device of claim 1,

the grinding end is configured to be capable of performing linear motion along the first axis a in a direction far away from the filter screen (201) and move from the filtering position to a limiting position, and the grinding end in the limiting position can be in limiting abutment with the filter screen (201) so that the grinding end can drive the filter screen (201) to perform synchronous movement along the first axis a.

3. The tissue dissociation device of claim 2,

the grinding end comprises a grinding rotor (202), a grinding stator (203) and a fixing piece (204), a rotating shaft hole (2033) penetrates through the grinding stator (203) along the direction of the first axis a, and a grinding surface (2031) is arranged on the grinding stator (203);

the grinding rotor (202) comprises a rotor shaft (2021) and a grinding blade (2022), one end of the grinding blade (2022) is connected to the middle part of the rotor shaft (2021), the first end of the rotor shaft (2021) is used for connecting a driving component, the second end of the rotor shaft (2021) movably penetrates through the rotating shaft hole (2033) so that the other end of the grinding blade (2022) is contacted with the grinding surface (2031) of the grinding stator (203), and the grinding blade (2022) can grind the biological tissue on the grinding surface (2031) when the grinding rotor (202) is configured to rotate;

the middle part of filter screen (201) is provided with the through-hole, the link of mounting (204) can pass the through-hole with the second end of rotor shaft (2021) is connected, just be provided with on mounting (204) and can pass the through-hole and with grind stator (203) and carry out spacing first limit structure, the spacing end of mounting (204) is provided with second limit structure, second limit structure can be spacing filter screen (201) is kept away from grind one side of stator (203).

4. The tissue dissociation device of claim 3,

the fixing piece (204) is a stepped shaft, the outer diameter of the stepped shaft is smaller than the size of the through hole, the stepped shaft comprises a small-diameter shaft (2042) and a large-diameter shaft (2043) which are coaxially connected, one end, far away from the large-diameter shaft (2043), of the small-diameter shaft (2042) is connected with the second end of the rotor shaft (2021), and the first limiting structure is formed between the small-diameter shaft (2042) and the large-diameter shaft (2043) to form a first limiting surface (2041);

the pivot hole (2033) is the shoulder hole, and it is including the small aperture hole and the big aperture hole that connect gradually, big aperture hole is close to filter screen (201) sets up, small aperture hole with form spacing face of second (2034) between the big aperture hole, first spacing face (2041) can with the spacing butt of spacing face of second (2034).

5. The tissue dissociation device of claim 4,

the second limit structure is a limit boss (2044), the limit boss (2044) is arranged at one end of the large-diameter shaft (2043) far away from the small-diameter shaft (2042), and the size of the limit boss (2044) is larger than that of the through hole.

6. The tissue dissociation device of claim 3,

the grinding surface (2031) is a conical surface, the other end of the grinding blade (2022) is obliquely provided with a grinding blade edge, and the grinding blade edge can move on the conical surface to cut the biological tissue.

7. The tissue dissociation device of claim 3,

the grinding surface (2031) is provided with a plurality of grinding grooves (2032), and the grinding grooves (2032) rotate around the first axis a.

8. The tissue dissociation device of claim 1,

a positioning step (103) is arranged on the inner wall surface of the sample bottle (100), and the filter screen (201) can be placed on the positioning step (103).

9. The tissue dissociation device of claim 3,

a third limiting structure is arranged between the grinding stator (203) and the sample bottle (100) to limit the grinding stator (203) to rotate around the first axis a.

10. The tissue dissociation device of claim 9,

the third limiting structure comprises a limiting block (104) arranged on the inner wall surface of the sample bottle (100) and a limiting groove arranged on the outer wall surface of the grinding stator (203), and the limiting block (104) can be inserted into the limiting groove.

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