CN218938289U - Sample injection module and single-molecule immunity analyzer comprising same - Google Patents

Abstract

The utility model relates to a sample introduction module and contain its monomolecular immunity analysis appearance, sample introduction module contains 2 ~ 4 layers of sample frames, and each layer of sample frame is parallel arrangement and the structure is the same in vertical direction, contains micropore board, microscope carrier, linear reciprocating motion mechanism respectively, linear reciprocating motion mechanism contains linear guide, actuating mechanism, drawer slide rail, hold-in range, action wheel, follow driving wheel and zero sensor. The single-molecule immunity analyzer comprises a sample injection module, a detection module, an incubation module, a reagent processing module and the like. The sample injection module can effectively reduce the volume of the module while ensuring the storage capacity of a sample, and the single-molecule immunity analyzer has the advantages of higher degree of automation, compact structure, low operation difficulty, improved detection efficiency and capability of reaching more than 90 test/hour.

Description

Sample injection module and single-molecule immunity analyzer comprising same

Technical Field

The present application relates to medical devices, and more particularly, to a sample injection module and a single molecule immunoassay analyzer comprising the same.

Background

An immunoassay device is a device for quantitatively analyzing a target analyte such as an antibody or an antigen contained in a test sample such as blood. In recent years, to meet the requirements of hypersensitive detection, single-molecule immunodetection methods have been developed. The single molecule immunization apparatus available on the market mainly comprises Simoa-HD-1 and HD-X of Quantix company in the United states. However, simoa-HD-1 and HD-X are limited by their principle, resulting in a large device size, HD-X being 135X 60X 160cm in size and very large. There is a need for a single molecule immunoassay that can reduce the volume while satisfying single molecule hypersensitivity detection.

The sample injection module is an integral module of an immunoassay analyzer, and the current sample injection module basically comprises a single sample rack or a plurality of sample racks arranged side by side or in series in a pipeline manner, and the latter can enlarge the sample loading amount, but the volume of the sample injection module is larger, so that the device is not compact enough (see patent documents 1-3).

Prior art literature

Patent document 1: CN205786676U

Patent document 2: CN211348257U

Patent document 3: CN113267639A

Disclosure of Invention

In view of the above problems, an object of the present application is to provide a sample injection module suitable for single-molecule immunoassay capable of effectively reducing the module volume while ensuring a large sample loading amount, and a single-molecule immunoassay analyzer including the same.

The application comprises the following technical scheme.

In a first aspect, the present application relates to a sample injection module, which comprises 2-4 layers of sample frames, wherein each layer of sample frame is arranged in parallel in the vertical direction and has the same structure, each layer of sample frame comprises a micro-pore plate, a carrier and a linear reciprocating mechanism from top to bottom, and the linear reciprocating mechanism is used for enabling the micro-pore plate to move back and forth in the sample injection direction.

In one embodiment, the distance between adjacent sample rack layers is 30-100 mm, and the gap between the lowest end of the upper layer of sample rack and the uppermost end of the lower layer of sample rack is 5-15 mm.

In one embodiment, the aforementioned microwell plate is a 48, 96, or 384 well plate.

In one embodiment, the sample introduction module comprises a 2-layer sample rack.

In one embodiment, the linear reciprocating mechanism includes: the linear guide rail mounting plate is positioned at the lower part of the linear guide rail and used for supporting the linear guide rail; two linear guide rails which are arranged at the lower part of the carrier and are in slidable contact with the left end and the right end of the carrier for the carrier to linearly move; the zero sensor is positioned on the linear guide rail mounting plate and positioned on the side surface of the linear guide rail and is used for providing an initial position for the sample rack and preventing the sample rack from position deviation; the driving mechanism is positioned below the linear guide rail mounting plate and is connected with the driving wheel to provide power; the drawer slide rail is positioned on the side surface of the linear guide rail mounting plate and is used for fixing the single-layer sample rack and removing the single-layer sample rack when the sample is replaced; the synchronous belt is positioned on the side surface of one linear guide rail and used for driving the carrying platform and the micropore plate to slide on the linear guide rail; the driving wheel is positioned at the tail end of one linear guide rail and is connected with the driving mechanism; and a driven wheel located at the other end of one linear guide rail.

In one embodiment, the aforementioned linear reciprocating mechanism further comprises: a synchronous belt pressing plate which clamps the synchronous belt; the synchronous belt adapter plate is fixed on the carrier and connected with the synchronous belt pressing plate, and the synchronous belt adapter plate and the synchronous belt pressing plate assist the synchronous belt to drive the carrier and the micro-pore plate to slide on the linear guide rail; and the zero sensor baffle is connected with the carrier and used for assisting the zero sensor to provide an initial position for the sample rack.

In another aspect, the present application relates to a single molecule immunoassay analyzer comprising: the sample injection module is used for injecting the sample; the sample needle module is used for extracting a sample from the sample injection module to the incubation module, and moving to a sample needle cleaning position for cleaning after the sample is sampled; the cup grabbing manipulator module is used for enabling the reaction cup to be transferred between the consumable module and the incubation module; a consumable module for storing consumables; the detection needle module is used for extracting the reactant after the uniform mixing and the reaction to the detection module, and moving the reactant to a detection needle cleaning position after the detection is finished; the detection module is used for detecting the extracted reactant and outputting a single-molecule signal; the incubation module is used for placing the reaction cup and carrying out uniform mixing and incubation operation on the liquid of the reaction cup; the reagent processing module is used for providing a constant temperature range for the reagent, uniformly mixing the reagent and inputting reagent information; and the reagent needle module is used for extracting the reagent in the reagent processing module to the incubation module, and moving the reagent to the reagent needle cleaning position after the extraction is completed.

In one embodiment, each of the incubation module and the reagent processing module includes at least one turntable, and a driving member for driving the turntable to rotate, wherein a plurality of placement grooves are formed on the turntable.

In one embodiment, the sample needle module includes a sample needle, an X-axis module, and a Z-axis module disposed on the X-axis module and including a Z-axis motor, a Z-axis zero sensor, a sample needle anti-collision device, and a Z-axis motion mechanism.

In one embodiment, the aforementioned detection needle module comprises a detection needle, a Y-axis module and a Z-axis module, wherein the aforementioned Y-axis module comprises a Y-axis motor, a Y-axis zero sensor, and a Y-axis motion mechanism, and the aforementioned Z-axis module is disposed on the Y-axis module and comprises a Z-axis motor, a Z-axis zero sensor, a detection needle anti-collision device, and a Z-axis motion mechanism.

In one embodiment, the reagent needle module includes a reagent needle having a liquid level detection function, an X-axis module including an X-axis motor, an X-axis zero sensor, and an X-axis movement mechanism, and a Z-axis module disposed on the X-axis module and including a Z-axis motor, a Z-axis zero sensor, a reagent needle collision prevention device, and a liquid level detection plate.

In one embodiment, the detection module includes an optical detection module (such as a CCD detection module), a measurement chamber assembly, and a control assembly including a focusing assembly and a shock absorbing assembly.

Compared with the prior art, the application can obtain the following excellent effects:

(1) By making each layer of sample rack comprise a micro-pore plate, a carrying platform and a linear reciprocating motion mechanism, large sample storage capacity is realized by a simple structure;

(2) By arranging the multi-layer sample racks, the storage amount of the samples is further increased, other sample racks in an idle state can be replaced in the running process of one layer of sample rack, continuous sample injection can be realized without stopping the machine, and the detection rate is accelerated;

(3) The volume occupied by the sample injection module is effectively reduced by the multi-layer stacked structure, so that the device is more compact, and the size of the single-molecule immunity analyzer can be in the range of 700-900 mm long by 600-800 mm wide by 600-800 mm high, and is far smaller than that of the existing single-molecule immunity analyzer;

(4) The single-molecule immunity analyzer has the advantages of higher automation degree, low operation difficulty, improved detection efficiency and capability of reaching more than 90 test/hour.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered limiting the scope, and that other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

Fig. 1 (a) is a schematic structural diagram of a sample injection module (a double-layer sample rack) according to an embodiment of the present application, and fig. 1 (b) is a schematic structural diagram of a single-layer sample rack according to an embodiment of the present application.

Fig. 2 (a) is a perspective view of a single-molecule immunoassay analyzer according to an embodiment of the present application, and fig. 2 (b) is a top view of the single-molecule immunoassay analyzer according to an embodiment of the present application.

Fig. 3 is a perspective view of a sample needle module of a single molecule immunoassay analyzer.

Fig. 4 is a perspective view of a cuvette handling manipulator module of a single molecule immunoassay analyzer.

Fig. 5 (a) is a top view of a consumable module of the single-molecule immunoassay analyzer, and fig. 5 (b) is a perspective view of the consumable module of the single-molecule immunoassay analyzer.

Fig. 6 is a perspective view of a detector pin module of a single molecule immunoassay analyzer.

FIG. 7 is a schematic diagram of a detection module of a single molecule immunoassay analyzer.

Fig. 8 is a top view of an incubation module of a single molecule immunoassay analyzer.

Fig. 9 is a perspective view of a reagent processing module of a single molecule immunoassay analyzer.

Fig. 10 is a perspective view of a reagent needle module of a single molecule immunoassay analyzer.

Reference numerals: 100-sample injection module; 110-sample rack; 120-fixing a bracket; 102-microwell plates; 103-a carrier; 104-a linear reciprocating mechanism; 105-a linear guide mounting plate; 106, a linear guide rail; 107-zero sensor; 108-a driving mechanism; 109-a driving wheel; 111-drawer slides; 112-a synchronous belt; 113-driven wheel; 114-a synchronous belt pressing plate; 115-a synchronous belt adapter plate; 116-zero sensor blade; 200-sample needle module; 300-a cup grabbing manipulator module; 400-consumable module; 500-detecting needle module; 600-a detection module; 700-incubation module; 800-a reagent processing module; 900-reagent needle module; 10-sample needle cleaning position; 20-a reagent needle cleaning position; 30-detecting a needle cleaning position;

201-sample needle; a 202-X axis module; 203-Z axis module; 204-sample needle bump guard;

301-a manipulator X-axis motion assembly; 302-a robot Y-axis motion assembly; 303-a manipulator Z-axis movement assembly; 304-a cup grabbing manipulator clamping assembly;

401-a consumable rack; 402-throwing a cup mouth; 403-reaction cup tray; 404-reaction cup;

501-a detection needle; 502-Y axis module; 503-Z axis module; 504-detecting a needle bump guard;

601-an optical detection module; 602-a measurement chamber assembly; 603-a control assembly;

701-mixing component; 702-incubating the tray body module; 703-a reaction cup storage tank;

801-a reagent cartridge body; 802-a refrigeration and heat dissipation assembly; 803, a code scanning defogging assembly; 804-a rotary motion mechanism;

901-a reagent needle; 902-Z axis module; 903-X axis module

Detailed Description

The terms "first," "second," "third," and the like are used merely for distinguishing between descriptions and not for indicating a sequence number, nor are they to be construed as indicating or implying relative importance.

Furthermore, the terms "horizontal," "vertical," "overhang," and the like do not denote a requirement that the component be absolutely horizontal or overhang, but rather may be slightly inclined. As "horizontal" merely means that its direction is more horizontal than "vertical", and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the present application, it should be noted that, directions or positional relationships indicated by terms such as "inner", "outer", "left", "right", "upper", "lower", etc. are directions or positional relationships based on those shown in the drawings, or those that are conventionally put in use for the product of the application, are merely for convenience of description and simplification of the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be configured and operated in a specific direction, and therefore should not be construed as limiting the present application.

In the description of the present application, unless explicitly stated and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements.

The technical solutions of the present application will be clearly and completely described below with reference to the accompanying drawings.

Fig. 1 is a schematic structural diagram of a sample injection module 100 according to an embodiment of the present application, where (a) is a schematic structural diagram of a dual-layer sample rack. Wherein each layer of sample holders 110 is identical in structure, and is disposed in parallel in the vertical direction, and the movement of each layer of sample holders is independent. The distance between adjacent sample rack layers and the gap between the lowest end of the upper layer of sample rack and the highest end of the lower layer of sample rack can be determined according to the total height of the analyzer to be designed and the sample taking convenience, and the inventor of the application finds that when the distance between the adjacent sample rack layers is set to be 30-100 mm and the gap between the lowest end of the upper layer of sample rack and the highest end of the lower layer of sample rack is set to be 5-15 mm, the reduction of the volume of the device and the accuracy of sampling can be simultaneously considered. The sample holders of each layer are slidably coupled to the fixing brackets 120 provided at the outer side, and the slidably coupling may be implemented in various manners, such as screw coupling. The number of the fixing brackets is even, and the number of the fixing brackets can be 2, 4, 6 and the like. The sample holder shown in fig. 1 (a) is double-layered, but may be 3 or 4 layers. Each layer of sample rack moves between a microplate loading position and a sample needle sampling position.

Fig. 1 (b) is a schematic structural diagram of a single-layer sample rack according to an embodiment of the present application. As shown in fig. 1 (b), each layer of sample rack includes a microplate 102, a carrier 103 and a linear reciprocating mechanism 104 from top to bottom. The linear reciprocating motion mechanism is used for enabling the micro-pore plate to move back and forth in the sample injection direction and comprises: a linear guide mounting plate 105 located at the lower portion of the linear guide 106 for supporting the linear guide 106; two linear guides 106 provided at the lower part of the stage 103 and slidably contacting both left and right ends of the stage 103, for linearly moving the stage 103; a zero sensor 107 located on the linear guide mounting plate 105 and located on a side of the linear guide 106 for providing an initial position to the sample rack 110 to prevent the sample rack from being deviated; a driving mechanism 108 located below the linear guide mounting plate 105 and connected to the driving wheel 109 to supply power; a drawer slide 111 located at a side of the linear guide mounting plate 105 for fixing the single-layer sample rack 110 and removing the single-layer sample rack when changing samples; and a timing belt 112 located on a side of one linear guide for driving the stage 103 and the microplate 102 to slide on the linear guide 106; a driving wheel 109, which is located at the end of one linear guide rail and is connected with the driving mechanism; and a driven wheel 113 located at the other end of one of the linear guides.

The linear guide mounting plate 105 is connected with the fixed support 120 through the drawer slide rail 111, so that a drawer type push-pull function is realized, samples of each layer can be conveniently and rapidly replaced under the condition that sampling of each layer is not affected, and a sample frame lifting mechanism does not need to be arranged to enable the sample frame to lift, so that the equipment structure is simpler. The drawer slide 111 has two pieces, and is respectively disposed on the left and right sides of the linear guide mounting plate. In each layer of sample rack, only one synchronous belt 112 is required to be arranged on the side surface of one linear guide rail, and in addition, only one driving wheel 109 and one driven wheel 113 are required to be arranged at the two tail ends of the linear guide rail 106 respectively.

The linear reciprocating mechanism may further comprise a timing belt pressing plate 114, a timing belt adapter plate 115, and a zero sensor blocking piece 116. The synchronous belt pressing plate 114 clamps the synchronous belt 112, and the synchronous belt adapter plate 115 is fixed on the carrier 103 and connected with the synchronous belt pressing plate 114, and assists the synchronous belt 112 together with the synchronous belt pressing plate 114 to drive the carrier 103 and the micro-pore plate 102 to slide on the linear guide rail 106. The zero sensor tab 116 is coupled to the stage 103 for assisting the zero sensor 107 in providing an initial position to the sample rack.

According to the utility model, the multi-layer sample rack is arranged, so that the sample storage capacity is further increased, other sample racks in an idle state can be replaced in the operation process of one layer of sample rack, continuous sample injection can be realized without stopping, and the detection rate is accelerated. The volume occupied by the sample injection module is effectively reduced due to the multi-layer stacked structure, so that the device is more compact.

Fig. 2 is a perspective view and a plan view of a single molecule immunoassay analyzer according to an embodiment of the present application. As shown in fig. 2 (a), the single-molecule immunoassay analyzer includes a sample needle module 200, a cuvette handling robot module 300, a consumable module 400, a detection needle module 500, a detection module 600, an incubation module 700, a reagent processing module 800, and a reagent needle module 900, in addition to the sample injection module 100. The sample needle module 200 is used for extracting a sample from the sample injection module 100 to the incubation module 700, moving to the sample needle cleaning position 10 for cleaning after sampling, the reagent needle module 900 is used for extracting a reagent in the reagent processing module 800 to the incubation module 700, moving to the reagent needle cleaning position 20 after extracting, the detection needle module 50 is used for extracting a reactant after mixing and reaction to the detection module 600, and moving to the detection needle cleaning position 30 after detection. The cuvette handling robot module is used to transfer cuvettes between the consumable module 400 and the incubation module 700. The single molecule immunoassay analyzer also includes a housing 40 for placement of the modules.

In fig. 2 (a), the sample injection module 100 is disposed at the leftmost side of the analyzer, and the consumable modules 400 are adjacently disposed along the length direction of the analyzer. The sample needle module 200, the detection needle module 500 and the reagent needle module 900 are all arranged at the rear part of the analyzer and are respectively positioned above the sample injection module 100, the incubation module 700 and the reagent processing module 800, so that sampling is facilitated. Further, around the incubation module 700, a sample needle washing station 10, a reagent needle washing station 20, and a detection needle washing station 30 are provided, and a solvent for washing each needle is provided in each washing station. By providing wash stations (10, 20, and 30) around the incubation module 700, the distance of movement of each needle can be minimized, the detection rate can be increased, and the apparatus can be made as compact as possible, reducing volume. A cup gripping robot module 300 is provided above the consumable module 400.

Fig. 2 (b) is a top view of a single molecule immunoassay analyzer according to an embodiment of the present application. As shown in fig. 2 (b), the detection module 600 is disposed adjacently to the rear of the incubation module 700, and the detection needle washing site 30 is located between the incubation module 700 and the detection module 600, so that the movement distance of the detection needle can be shortened, and the detection efficiency can be improved. The detection module 600 is configured to detect the extracted reactant and output a single molecule signal.

The following describes each module of the single molecule immunoassay analyzer except the sample injection module 100.

Fig. 3 is a perspective view of a sample needle module 200 of a single molecule immunoassay analyzer. The sample needle module comprises a sample needle 201, an X-axis module 202 and a Z-axis module 203, wherein the Z-axis module 203 is arranged on the X-axis module 202 and comprises a Z-axis motor, a Z-axis zero sensor, a sample needle anti-collision device 204 and a Z-axis movement mechanism. In the figure, sample needle buffer stop is used for preventing the vertical Z axle collision of application of sample needle, when the needle is in the Z axle direction when touching hard thing because of unexpected circumstances such as equipment outage, maloperation etc. lead to, sample needle buffer stop can make sample needle automatic re-setting to avoid causing harmful effects to equipment continuation operation, play the cushioning effect simultaneously, prevent that the needle from being directly bumped bad, reduce the spoilage. Here, the sample needle bump guard is a bump spring, but is not limited thereto. The X-axis module and the Z-axis module are respectively used for enabling the sample needle to move in the X-axis direction and the Z-axis direction, and can comprise a motor, a guide rail, a sliding block, a belt pulley and other parts as a movement mechanism, and a zero sensor is further arranged in the Z-axis module.

Fig. 4 is a perspective view of a cuvette handling manipulator module 300 of a single molecule immunoassay analyzer. The cup grabbing manipulator module 300 can move in the directions of the three axes XYZ and has the functions of cup taking, cup placing and cup throwing. The cup grabbing manipulator gripper assembly 304 is disposed on the manipulator Z-axis movement assembly 303. The robot Z-axis movement assembly 303 can drive the gripper assembly 304 to move back and forth in the Z-direction, which is provided on the robot Y-axis movement assembly 302. The manipulator Y-axis motion assembly 302 can drive the manipulator Z-axis motion assembly 303 and the cup grabbing manipulator gripper assembly 304 to move back and forth in the Y-direction, and is disposed on the manipulator X-axis motion assembly 301. The manipulator X-axis motion assembly 301 can drive the manipulator Y-axis motion assembly 302, the manipulator Z-axis motion assembly 303 and the cup grabbing manipulator clamping assembly 304 to move back and forth in the X direction. In this way, a triaxial movement is achieved. The manipulator X-axis movement assembly 301, the manipulator Y-axis movement assembly 302, and the manipulator Z-axis movement assembly 303 may include a motor, a guide rail, a slider, a screw, a pulley, a sprocket, or the like. The cup grasping manipulator gripper assembly 304 may be either electric or pneumatic.

Fig. 5 is a top view and a perspective view of a consumable module 400 of a single molecule immunoassay analyzer, the consumable module comprising two consumable holders 401, and a cup throwing opening 402. Two consumable racks 401 are respectively provided with a reaction cup tray 403 for storing reaction cups 404, and a cup throwing opening 402 is arranged on one side of the consumable rack and is used for discharging the used reaction cups.

Fig. 6 is a perspective view of a detection needle module 500 of a single molecule immunoassay analyzer, the detection needle module 500 including a detection needle 501, a Y-axis module 502, and a Z-axis module 503. Wherein, Z-axis module 503 is disposed on Y-axis module 502, and there is a detection needle anti-collision device 504. The detecting needle anti-collision device 504 is used for preventing the detecting needle 501 from collision along the vertical Z axis, here, but not limited to, an anti-collision spring.

Fig. 7 is a side view and a perspective view of a detection module 600 of a single molecule immunoassay analyzer. The detection module 600 includes an optical detection module (e.g., a CCD detection module) 601, a measurement chamber assembly 602, and a control assembly 603, which may include a focusing assembly, a shock absorbing assembly, and the like. The optical detection module 601 is used for detecting a measurement area, the measurement chamber assembly 602 has XY axis direction position calibration and magnetic adsorption functions, and the control assembly 603 has functions of reducing vibration of the measurement assembly and adjusting focusing distance.

Fig. 8 is a top view of an incubation module 700 of a single molecule immunoassay analyzer. The incubation module 700 comprises a mixing assembly 701 and an incubation tray body module 702. The mixing assembly 701 has a function of mixing the sample with the reagent, and includes a mixing wheel, a driving mechanism (e.g., a motor), and the like. As shown in fig. 8, the mixing is performed by directly driving a mixing wheel, which is in contact with the incubation plate at a mixing position, through a driving mechanism (e.g., a motor). A plurality of reaction cup storage grooves 703 are provided in the incubation plate body module 702, and a heating plate (which may be a heating film) is installed at the lower portion of the incubation plate for maintaining the temperature of the incubation plate in a constant range (e.g., 40±0.5 ℃). The incubation module 700 is provided with a mixing position, a detection position and a reaction cup placement position.

Fig. 9 is a perspective view of a reagent processing module 800 of a single molecule immunoassay analyzer. The reagent processing module 800 comprises a reagent bin main body 801, a refrigerating and radiating assembly 802, a code scanning and demisting assembly 803 and a rotary motion mechanism 804, and is used for providing a constant temperature storage range (such as 2-8 ℃ +/-0.5 ℃) for a reagent, uniformly mixing the reagent and inputting reagent information. The reagent bin main body 801 comprises a mixing disc, a heat radiation component (such as a heat radiation fan and an inverted cooling block), a zero sensor, a mixing stirring component, a mixing gear, a fixed spur gear, a bearing and a driven wheel, and is mainly used for storing and uniformly mixing reagents. The code scanning defogging component 803 comprises a defogging heating component and a code scanning device, and is mainly used for collecting reagent information, and the defogging heating component can comprise a heating film and organic glass. The cooling and heat dissipating component 802 is used for cooling the reagent cabin main body 801, and may be disposed at a lower portion of the reagent cabin main body 801, and may include a cooling fan, a cooling fin, and a cooling air duct inlet. The rotary motion mechanism 804 may comprise a rotary motor, a zero sensor, a synchronous belt, a driving wheel and a driven wheel, and is located at the lower part of the cooling and heat dissipating assembly 802.

Fig. 10 is a perspective view of a reagent needle module 900 of a single molecule immunoassay analyzer. The reagent needle module 900 includes a reagent needle 901 having a liquid level detecting function, a Z-axis module 902 and an X-axis module 903, wherein the Z-axis module 902 is disposed on the X-axis module 903 and includes a reagent needle anti-collision device 904, and has a reagent needle anti-collision function. When the reagent needle 901 with the liquid level detection function is adopted, the detection function for the liquid level of the reagent is provided, and the precision in repeated extraction can be improved. The reagent needle module 900 is used for extracting various reagents in the reagent processing module 800 to the incubation module 700, and after each extraction, the reagent needle 901 moves to the reagent needle cleaning position 20 for cleaning.

This application adopts pipelined structure through setting up above-mentioned module to can realize that the application of sample, incubation, reagent, mixing, washing and detection integration operation, degree of automation is higher, has reduced the operation degree of difficulty, and has improved detection efficiency.

Hereinafter, specific working steps will be described by taking a single reaction cup as an example, and this is merely an example and should not be construed as limiting the present utility model.

A: the cup grabbing mechanical arm clamping assembly of the cup grabbing mechanical arm module is used for placing the reaction cup in the consumable module at the cup placing position of the reaction cup of the incubation module;

b: the multi-layer sample rack of the sample module is loaded with a 96-well plate containing samples; the 96-well plate moves from a sample replacing position to a sample extracting position of the sample needle module, and simultaneously, a sample needle of the sample needle module moves to the same position to extract a sample;

c: the sample needle moves to a sample adding position of the incubation module, the incubation module moves the reaction cup which completes the action A to the sample adding position, and the sample needle adds a sample to the reaction cup; after the addition is completed, the sample needle moves to a sample needle cleaning position for cleaning;

d: the reagent processing module scans the code component and inputs the kit information, and the reagent needle module moves to the reagent extraction position to extract the reagent R1, and simultaneously moves the reaction cup which completes the action C to the mixing position of the incubation module. The reagent needle module moves to a mixing position, and R1 is added; at the moment, the incubation module is heated for incubation, the reagent needle module moves to a reagent needle cleaning position for cleaning, and a mixing component at the mixing position uniformly mixes the reaction cup added with the reagent R1;

e: the reagent processing module scans the code component to input the information of the reagent kit, the reagent needle module moves to the reagent extraction position to extract the reagent R2, and simultaneously the reaction cup which completes the action D moves to the mixing position of the incubation module, and the reagent needle module moves to the mixing position to add the reagent R2; at the moment, the incubation module is heated for incubation, the reagent needle module moves to a reagent needle cleaning position for cleaning, and a mixing component at the mixing position uniformly mixes the reaction cup added with the reagent R2;

f: after incubation is completed, the incubation module moves the reaction cup with the reaction completed to a detection position, and meanwhile, the detection needle of the detection needle module moves to the detection position, so that the object to be detected is extracted.

G: the detection needle module moves the extracted object to be detected to a flow cell in a measuring chamber component of the detection module, the object to be detected is detected and photographed through the optical detection module, and after detection is completed, the detection needle module moves to a detection needle cleaning position for cleaning.

H: and (3) taking out the reaction cup which is reacted from the cup placing position (step A) of the reaction cup of the incubation module by the cup grabbing mechanical arm module, moving the reaction cup to a cup throwing opening of the consumable module, and throwing the waste cup.

The reaction cups are orderly carried out without interference, thus realizing continuous and automatic detection. In the use process, the sample needle, the reagent needle and the detection needle can be respectively cleaned at the sample needle cleaning position, the reagent needle cleaning position and the detection needle cleaning position. So this application adopts pipelined structure to can realize that the application of sample, incubation, add the reagent, mixing, wash and detect integrated operation, degree of automation is higher, has reduced the operation degree of difficulty, and has improved detection efficiency.

The foregoing is merely a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and variations may be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims (12)

1. The sample injection module is characterized by comprising 2-4 layers of sample frames,

each layer of sample frame is arranged in parallel in the vertical direction and has the same structure, each layer of sample frame comprises a micro-pore plate, a carrying platform and a linear reciprocating mechanism from top to bottom, and the linear reciprocating mechanism is used for enabling the micro-pore plate to move back and forth in the sample feeding direction.

2. The sample injection module of claim 1, wherein the distance between adjacent sample rack layers is 30-100 mm, and the gap between the lowest end of the upper layer of sample rack and the uppermost end of the lower layer of sample rack is 5-15 mm.

3. The sample injection module of claim 1 or 2, wherein the microwell plate is a 48, 96, or 384 well plate.

4. The sample introduction module of claim 1 or 2, comprising a 2-layer sample rack.

5. The sample injection module of claim 1 or 2, wherein the linear reciprocating mechanism comprises: the linear guide rail mounting plate is positioned at the lower part of the linear guide rail and used for supporting the linear guide rail; two linear guide rails which are arranged at the lower part of the carrier and are in slidable contact with the left end and the right end of the carrier for the carrier to linearly move; the zero sensor is positioned on the linear guide rail mounting plate and positioned on the side surface of the linear guide rail and is used for providing an initial position for the sample rack and preventing the sample rack from position deviation; the driving mechanism is positioned below the linear guide rail mounting plate and is connected with the driving wheel to provide power; the drawer slide rail is positioned on the side surface of the linear guide rail mounting plate and is used for fixing the single-layer sample rack and removing the single-layer sample rack when the sample is replaced; the synchronous belt is positioned on the side surface of one linear guide rail and used for driving the carrying platform and the micropore plate to slide on the linear guide rail; the driving wheel is positioned at the tail end of one linear guide rail and is connected with the driving mechanism; and a driven wheel located at the other end of one linear guide rail.

6. The sample injection module of claim 1 or 2, wherein the linear reciprocating mechanism further comprises: a synchronous belt pressing plate which clamps the synchronous belt; the synchronous belt adapter plate is fixed on the carrier and connected with the synchronous belt pressing plate, and the synchronous belt adapter plate and the synchronous belt pressing plate assist the synchronous belt to drive the carrier and the micro-pore plate to slide on the linear guide rail; and the zero sensor baffle is connected with the carrier and used for assisting the zero sensor to provide an initial position for the sample rack.

7. A single molecule immunoassay analyzer comprising: the sample introduction module of any one of claims 1 to 6; a sample needle module for extracting a sample from the sample injection module to the incubation module; the cup grabbing manipulator module is used for enabling the reaction cup to be transferred between the consumable module and the incubation module; a consumable module for storing consumables; the detection needle module is used for extracting the reactant after the uniform mixing and the reaction to the detection module; the detection module is used for detecting the extracted reactant and outputting a single-molecule signal; the incubation module is used for placing the reaction cup and carrying out uniform mixing and incubation operation on the liquid of the reaction cup; the reagent processing module is used for providing a constant temperature range for the reagent, uniformly mixing the reagent and inputting reagent information; and a reagent needle module for extracting the reagent in the reagent processing module to the incubation module.

8. The single molecule immunoassay apparatus according to claim 7, wherein each of said incubation module and said reagent processing module comprises at least one turntable, and a driving member for driving said turntable to rotate, said turntable having a plurality of placement grooves thereon.

9. The single molecule immunoassay analyzer of claim 7 or 8, wherein said sample needle module comprises a sample needle, an X-axis module and a Z-axis module, said Z-axis module being disposed on said X-axis module and comprising a Z-axis motor, a Z-axis zero sensor, a sample needle anti-collision device and a Z-axis motion mechanism.

10. The single molecule immunoassay analyzer of claim 7 or 8, wherein said detector needle module comprises a detector needle, a Y-axis module and a Z-axis module, said Y-axis module comprising a Y-axis motor, a Y-axis zero sensor, and a Y-axis motion mechanism, said Z-axis module being disposed on said Y-axis module and comprising a Z-axis motor, a Z-axis zero sensor, a detector needle anti-collision device, and a Z-axis motion mechanism.

11. The single molecule immunoassay analyzer of claim 7 or 8, wherein said reagent needle module comprises a reagent needle having a liquid level detection function, an X-axis module comprising an X-axis motor, an X-axis zero sensor, and an X-axis movement mechanism, and a Z-axis module disposed on the X-axis module and comprising a Z-axis motor, a Z-axis zero sensor, a reagent needle anti-collision device, and a liquid level detection plate.

12. The single molecule immunoassay analyzer of claim 7 or 8, wherein said detection module comprises an optical detection module, a measurement cell assembly, and a control assembly comprising a focusing assembly and a shock absorbing assembly.

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