SUMMERY OF THE UTILITY MODEL
The utility model provides a device is raised on a large scale to spot wing drosophila for overcome at least one technical problem who exists among the prior art.
The embodiment of the utility model provides a large-scale feeding device for spotted wing fruit flies, which comprises an insect feeding cage, a fruit fly bottle, wood wool and artificial feed; the four sides of the insect rearing cage are wrapped by nylon gauze; the fruit fly bottle is a transparent plastic bottle; the fruit fly bottle is arranged at the bottom of the insect rearing cage; the artificial feed is placed at the bottom in the fruit fly bottle; the wood wool is arranged in the bottle of the fruit fly bottle and is arranged on the upper layer of the artificial feed.
Optionally, the mesh size of the nylon gauze is 200-300 meshes.
Optionally, the shape of the fruit fly bottle is a cone with a narrow bottle mouth and a wide bottle bottom.
Optionally, the volume of the artificial feed is not more than 1/10 of the volume of the fruit fly bottle.
Optionally, the wood wool contained in the fruit fly bottle is 5-10.
Compared with the prior art, the utility model discloses following beneficial effect can be obtained:
the large-scale feeding device for the spotted wing fruit flies can provide more stop areas for the spotted wing fruit flies through the wood wool, and effectively prevents the spotted wing fruit flies from being stuck by feed; the fruit fly bottles are not sealed, so that the phenomenon that the male-female ratio difference of the spotted wing fruit flies in each single fruit fly bottle is too large can be avoided, and the spotted wing fruit flies can freely and movably propagate in the insect rearing cage; a plurality of feeding devices can be arranged, and only one bottle of feed with the spotted wing drosophila flies needs to be put into each feeding cage to propagate, so that population propagation is easier and quicker. The utility model has wide application range, and is also applicable to other drosophilae and partial hemiptera insects. Is one of the innovation points of the embodiment of the utility model.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without any creative effort belong to the protection scope of the present invention.
It should be noted that the terms "comprises" and "comprising" and any variations thereof in the embodiments and drawings of the present invention are intended to cover non-exclusive inclusions. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those steps or elements listed, but may alternatively include other steps or elements not listed, or inherent to such process, method, article, or apparatus.
The utility model provides a device is raised on a large scale of spotted wing fruit fly. Referring to fig. 1, fig. 1 is a schematic structural diagram of a large-scale feeding device for spotted wing drosophila according to an embodiment of the present invention.
As shown in fig. 1, the device includes an insect rearing cage 1, a drosophila bottle 2, wood wool 3, and artificial feed 4.
The four sides of the insect rearing cage 1 are wrapped by nylon gauze, and the mesh size of the nylon Long Shawang is 200-300 meshes. The fruit fly bottle 2 is a transparent plastic bottle, and the shape of the fruit fly bottle 2 is a cone with a narrow bottle mouth and a wide bottle bottom; the fruit fly bottle 2 is arranged at the bottom of the insect rearing cage 1. The artificial feed 4 is arranged at the bottom in the fruit fly bottle 2; the wood wool 3 is arranged in the bottle of the fruit fly bottle 2 and is arranged on the upper layer of the artificial feed 4.
Further, the artificial feed 4 is composed of corn flour, white granulated sugar, dry yeast, brown sugar, agar powder, peptone, cell-grade yeast powder, sorbic acid, 10% methylparaben, blackcurrant concentrated grape juice, propionic acid and sterilized water.
It can be understood that the mesh size of the nylon gauze of the insect rearing cage 1 can ensure ventilation, ventilation and dryness of the whole rearing environment, avoid the spotted wing fruit flies from being stuck due to overhigh humidity, and simultaneously can prevent the spotted wing fruit flies from flying out of the insect rearing cage. The conical shape of the fruit fly bottle 2 can make the area of the artificial feed 4 larger and thinner, so that the larva of the spotted wing fruit fly can easily climb out when pupating, and the feed can be replaced and the larva can be conveniently taken out when an experiment is carried out. The wood wool 3 is cheap and practical, can increase the rest area of the spotted wing fruit flies, and effectively prevents the spotted wing fruit flies from being stuck by the feed. The artificial feed 4 has the advantages of easily available raw materials, low cost, convenient preparation, comprehensive nutrient components, good corrosion prevention effect, good feeding effect, time and labor saving, easy operation, strong practicability and contribution to large-scale feeding of the drosophila melanogaster.
In specific implementation, the following components required by the artificial feed 4 are prepared firstly, and the actual dosage can be increased or decreased according to the formula proportion: 30-40g of corn flour, 15-20g of white granulated sugar, 10-15g of dry yeast, 6-10g of brown sugar, 5-8g of agar powder, 5-8g of peptone, 5-8g of cell-level yeast powder, 0.7-1g of sorbic acid, 20-30mL of 10% methyl p-hydroxybenzoate, 50-60mL of blackcurrant concentrated grape juice, 5mL of propionic acid and 500-600mL of sterilized water.
Firstly, putting all dry yeast into a pot, adding 300mL of sterile water to melt, boiling with strong fire, uniformly mixing corn flour, white granulated sugar, brown sugar, agar powder, peptone, cell-level yeast powder and sorbic acid in a container, pouring the mixture into the pot, adding the rest sterile water and all blackcurrant concentrated grape juice, uniformly mixing, stirring vigorously, and stewing slowly with slow fire for 10min.
And preparing 10% of methyl p-hydroxybenzoate in a second step, wherein the actual dosage can be increased or decreased in proportion by taking 1000mL as an example: 100g of methylparaben were weighed and completely dissolved in 950mL of absolute ethanol and 50mL of sterilized water.
And thirdly, cooling the cooked feed at room temperature for 5-10 min, adding 20-30mL of prepared 10% methyl p-hydroxybenzoate and 5mL of prepared propionic acid, and uniformly stirring. And pouring the prepared artificial feed 4 into a 250mL fruit fly bottle 2, wherein the volume of the poured feed cannot exceed 1/10 of the volume of the fruit fly bottle 2, otherwise, the larva in the feed which is too thick cannot climb out of the feed to pupate. After the feed is cooled and solidified, the feed is placed in a fume hood to be dried until no water vapor exists, then 5-10 wood filaments 3 are inserted into the artificial feed 4, the spotted wing fruit flies are placed into the fruit fly bottle 2, and then the fruit fly bottle 2 is placed into the insect rearing cage 1.
Finally, the insect rearing cage 1 is placed at the temperature of 24 +/-0.5 ℃ and the photoperiod of 16L:8D, breeding in an artificial incubator with the humidity of 55-65%. The artificial feed 4 is replaced every half month, when the artificial feed 4 is replaced, the fruit fly bottle 2 filled with the newly prepared feed is placed into the insect rearing cage 1 filled with the spotted wing fruit flies, then the spotted wing fruit flies in the old fruit fly bottle 2 are shaken out from the bottle, and all the fruit fly bottles 2 filled with the old feed are replaced in sequence.
Therefore, the large-scale feeding device for the spotted wing fruit flies, provided by the utility model, can provide more rest areas for the spotted wing fruit flies through the wood wool 3, and effectively avoid the spotted wing fruit flies from being stuck by the feed; the fruit fly bottles 2 are not sealed, so that the situation that the proportion difference of male and female parts of the spotted wing fruit flies in each single fruit fly bottle 2 is too large, and the spotted wing fruit flies can freely and movably propagate in the insect rearing cage 1 can be avoided; a plurality of feeding devices can be arranged, and only one bottle of feed with the spotted wing drosophila flies needs to be put into each feeding cage to propagate, so that population propagation is easier and faster. The utility model has wide application range, and is also applicable to other drosophilae and partial hemiptera insects.
Those of ordinary skill in the art will understand that: the figures are schematic representations of one embodiment, and the blocks or processes in the figures are not necessarily required to practice the present invention.
Those of ordinary skill in the art will understand that: modules in the devices in the embodiments may be distributed in the devices in the embodiments according to the description of the embodiments, or may be located in one or more devices different from the embodiments with corresponding changes. The modules of the above embodiments may be combined into one module, or further split into multiple sub-modules.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art will understand that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the embodiments of the present invention.