CN218250863U - Centrifugal device and system for extracting platelet-rich plasma - Google Patents
Centrifugal device and system for extracting platelet-rich plasma Download PDFInfo
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- CN218250863U CN218250863U CN202221549120.3U CN202221549120U CN218250863U CN 218250863 U CN218250863 U CN 218250863U CN 202221549120 U CN202221549120 U CN 202221549120U CN 218250863 U CN218250863 U CN 218250863U
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Abstract
The present application provides a centrifugation device and system for extracting platelet rich plasma, the centrifugation device comprising: the blood vessel comprises a tube body, a first tube and a second tube, wherein the interior of the tube body is of a hollow structure and is used for containing blood, the top of the tube body is of a first step structure, and the bottom of the tube body is of a second step structure; the first joint is arranged on the first step structure in a penetrating manner, the top of the first joint is detachably connected with a first pipe cover, and the first pipe cover is used for opening or closing the first joint; the second joint is arranged on the second step structure in a penetrating mode, the bottom of the second joint is detachably connected with a second pipe cover, and the second pipe cover is used for opening or closing the second joint. The application provides a centrifugal device and system for drawing rich platelet plasma, simple structure, convenient operation, platelet enrichment rate is high, reduces the loss of platelet, can effectively enrich three kinds of granulocytes and platelet plasma.
Description
Technical Field
The application relates to the technical field of medical equipment, in particular to a centrifugal device and a system for extracting platelet-rich plasma.
Background
Platelet-rich plasma (PRP) is a Platelet concentrate obtained by centrifuging autologous whole blood, and contains a large amount of growth factors and proteins. At present, platelet-rich plasma is applied to the fields of clinical oral cavity, plastic surgery, orthopedics, chronic unhealed wound and the like at home and abroad, and obtains better curative effect. The existing platelet-rich plasma centrifugal device is generally of a straight tube type structure, one end of the device is a blind end, the other end of the device can be opened and closed, platelets are in the middle layer of the centrifugal device according to density after centrifugal separation of a centrifugal machine, a suction tube needs to be used for stretching into the liquid level to suck the platelets, the operation is not convenient enough, the number of the extracted platelets can be reduced, and the platelet enrichment rate is low.
SUMMERY OF THE UTILITY MODEL
In view of the above, the present application is directed to a centrifuge apparatus and a system for extracting platelet-rich plasma, so as to solve the related problems mentioned in the background art.
In view of the above object, the present application provides, in a first aspect, a centrifuge device for extracting platelet rich plasma, comprising: the blood transfusion tube comprises a tube body, a first tube and a second tube, wherein the tube body is of a hollow structure and used for containing blood, the top of the tube body is of a first step structure, and the bottom of the tube body is of a second step structure; the first joint is arranged on the first step structure in a penetrating manner, the top of the first joint is detachably connected with a first pipe cover, and the first pipe cover is used for opening or closing the first joint; the second joint is arranged on the second step structure in a penetrating mode, the bottom of the second joint is detachably connected with a second pipe cover, and the second pipe cover is used for opening or closing the second joint.
Further, the first joint and the second joint have the same structure and are both luer cone locking cones.
Furthermore, the first pipe cover and the second pipe cover have the same structure and comprise a cover top and a cover body which are connected, a first connecting structure is arranged at the bottom of the cover body, and the first connecting structure is detachably connected with the first joint or the second joint.
Further, the centrifugation device for extracting platelet rich plasma further comprises: the two-way joint, inside is hollow structure, and one end is equipped with second connection structure, and the other end is equipped with third connection structure, second connection structure be used for with first joint or the connection can be dismantled to the second joint, third connection structure is used for dismantling with the syringe and is connected.
Furthermore, the pipe body is a cylinder, the diameter of the pipe body is 20-35mm, and the height of the pipe body is 35-100mm.
Further, first stair structure includes first step, first step is the cylinder, the diameter equals the diameter of body.
Furthermore, a second step which is communicated with the first step is arranged on the first step, the second step is a cylinder, the diameter of the second step is 6-16mm, and the height of the second step is 20-50mm.
Further, the second step structure comprises a third step and a fourth step which are communicated with each other, the third step and the fourth step are both cylinders, the diameter of the third step is equal to that of the tube body, the diameter of the fourth step is 6-18mm, and the height of the fourth step is 30-60mm.
Furthermore, a fifth step communicated with the fourth step is arranged on the fourth step, and the diameter of the fifth step is 6-11mm.
In a second aspect of the present application, there is provided a system for extracting platelet rich plasma comprising a centrifugation device as described above for the first aspect.
As can be seen from the above, the centrifugal device and the system for extracting platelet-rich plasma provided by the present application are provided with a tube for containing blood; the two ends of the tube body are of the stepped structure, so that the tube body is easy to settle on the steps of the stepped structure in the process of layering by means of density gradient during cell centrifugation, blood is not easy to form vortex to impact a platelet layer, cells are easy to form gradient arrangement, and enrichment and extraction of platelet-rich plasma are facilitated; the joints are arranged on the stepped structures at the two ends of the pipe body, and the corresponding pipe covers are arranged to match the joints, so that the two ends of the pipe body can be opened and closed, and when the pipe covers at the two ends are closed, the pipe body is closed and can be used for centrifugal operation; when the tube cover at one end is closed and the tube cover at the other end is opened, blood in the tube body cannot flow out of the joint due to the pressure effect, and the joint can be connected with other equipment; when the tube covers at the two ends are opened, blood can flow out of the tube body through the connectors, and then a red blood cell layer or a platelet layer and the like are discharged, so that the disturbance on layered blood is small, a suction tube does not need to be used to go deep into the liquid level to suck the platelet layer, the platelet layer is prevented from being scattered by tiny shaking in the sucking operation, and the number of the extracted platelets is increased; the side wall of the suction pipe is prevented from being stained with platelets to reduce the number of the extracted platelets, and the platelet enrichment rate is further improved; vortex impact on the platelet layer can be avoided when the suction pipe sucks the platelet, and the extraction concentration of the platelets can be further improved; the centrifugal device and the system for extracting the platelet-rich plasma have the advantages of simple structure, convenience in operation, high platelet enrichment rate, reduction in platelet loss and capability of effectively enriching three types of granulocytes and platelet plasma.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings used in the embodiments or the prior art descriptions will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic diagram of a centrifuge device for extracting platelet rich plasma according to an embodiment of the present application;
FIG. 2 is a schematic diagram of another centrifuge device for extracting platelet rich plasma according to an embodiment of the present application;
FIG. 3 is a schematic structural diagram of a first tube cover in an embodiment of the present application;
fig. 4 is a schematic structural diagram of a bidirectional connector in an embodiment of the present application.
Reference numerals are as follows: 1. a pipe body; 2. a first stepped structure; 2-1, a first step; 2-2, a second step; 3. a second stepped structure; 3-1, a third step; 3-2, a fourth step; 3-3, a fifth step; 4. a first joint; 5. a first tube cover; 5-1, capping; 5-2, a cover body; 5-3, a first connecting structure; 6. a second joint; 7. a second tube cover; 8. a bidirectional joint; 8-1, a second connecting structure; 8-2 and a third connecting structure.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the present application is further described in detail below with reference to the accompanying drawings in combination with specific embodiments.
It should be noted that technical terms or scientific terms used in the embodiments of the present application should have a general meaning as understood by those having ordinary skill in the art to which the present disclosure belongs, unless otherwise defined. The use of "first," "second," and similar terms in this disclosure is not intended to indicate any order, quantity, or importance, but rather is used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.
Platelet-rich plasma (PRP) is a Platelet concentrate obtained by centrifuging autologous whole blood, and contains a large amount of growth factors and proteins. At present, the platelet-rich plasma is applied to the fields of clinical oral cavity, plastic surgery, orthopedics, chronic non-healing wound and the like at home and abroad, and obtains better curative effect.
The platelet-rich plasma application technology evolves into two categories in continuous development, one category is plasma rich in platelets and free of leukocytes; the second type is plasma that is rich in platelets and also white blood cells. The cells in human blood are divided into three types, namely erythrocytes, leukocytes and platelets, wherein the leukocytes are divided into granulocytes and non-granulocytes, and the granulocytes are divided into neutrophils, eosinophils and basophils, and the three granulocytes play an important role in non-specific anti-infection immunity. The autologous plasma rich in granulocytes and platelets is clinically determined to be capable of effectively playing a local immune function aiming at wound infection, and specifically shows that chemotaxis, proliferation and differentiation, bacteria phagocytosis, angiogenesis, extracellular matrix production and the like can be realized in inflammatory tissues, and meanwhile, the rich platelets generate a large amount of high-concentration multiple growth factors to promote wound healing. The second kind of platelet rich plasma has double functions of resisting infection and promoting wound healing, and has excellent clinical application foreground.
The existing centrifugation device for the platelet-rich plasma is generally of a straight tube type structure, one end of the centrifugation device is a blind end, the other end of the centrifugation device can be opened and closed, platelets are centrifugally separated by a centrifuge and then are positioned in the middle layer of the centrifugation device according to density, the red blood cell layer is arranged below the centrifugation device, the supernatant layer is arranged above the centrifugation device, the platelets need to be extracted by stretching into the liquid level through a suction tube, the operation is not convenient enough, the stretched suction tube can disperse the platelet layer due to small shaking in the operation when absorbing the platelets, and the number of the extracted platelets is reduced; the side wall of the extended straw is also adhered with platelets to reduce the number of the finally extracted platelets, and the platelet enrichment rate is low; in addition, the existing centrifugal device easily forms liquid into vortex when centrifuging and sucking the platelet layer by using a suction pipe, and can impact the thin platelet layer to enable the platelets to be dispersed, and the extraction concentration of the platelets can be reduced.
The technical solution of the present application will be further described in detail by specific examples with reference to fig. 1 to 4.
Some embodiments of the present application provide a centrifugation device for extracting platelet rich plasma, as shown in fig. 1 and 2, comprising: the blood transfusion tube comprises a tube body 1, a first step structure 2 and a second step structure 3, wherein the interior of the tube body is of a hollow structure and is used for containing blood; the first joint 4 is arranged on the first step structure 2 in a penetrating manner, the top of the first joint is detachably connected with a first pipe cover 5, and the first pipe cover 5 is used for opening or closing the first joint 4; the second joint 6 is arranged on the second step structure 3 in a penetrating mode, the bottom of the second joint is detachably connected with a second pipe cover 7, and the second pipe cover 7 is used for opening or closing the second joint 6.
The centrifugal device is made of polypropylene or polyethylene and the like, and is not limited specifically, and the surface of the centrifugal device has the characteristic of hydrophobicity, so that the blood compatibility is better.
Body 1 is the cylinder, through setting up body 1 for hold blood, carry out centrifugal operation.
The top of body 1 is first stair structure 2, and the bottom is second stair structure 3, and the both ends of body 1 all set up to stair structure, and when centrifugation like this, the cell relies on the in-process of density gradient layering, subsides easily on stair structure's step, and blood is difficult to form the vortex and assaults the platelet layer, and the cell also forms gradient type easily and arranges, does benefit to the enrichment and the extraction of rich platelet plasma.
The first joint 4 is arranged on the first step structure 2 in a penetrating manner, the top of the first joint is detachably connected with a first pipe cover 5, and the first pipe cover 5 is used for opening or closing the first joint 4; the second joint 6 is arranged on the second stepped structure 3 in a penetrating manner, the bottom of the second stepped structure is detachably connected with a second pipe cover 7, and the second pipe cover 7 is used for opening or closing the second joint 6; the joints are arranged through the step structures at the two ends of the pipe body 1, and the corresponding pipe covers are matched and connected, so that the two ends of the pipe body 1 can be opened and closed, and the two ends of the pipe body 1 can be extracted.
When the tube covers at the two ends of the tube body 1 are closed, the tube body 1 is closed and can be placed into a centrifugal machine for centrifugal operation.
When the tube cap is closed at one end of the tube body 1 and is opened at the other end, blood in the tube body 1 cannot flow out of the connector due to the pressure effect, and the connector can be connected with other equipment, such as an injector, so that the subsequent operations of sucking a red blood cell layer or a platelet layer and the like are facilitated.
When tube covers at two ends of the tube body 1 are opened, the tube body 1 is communicated with the outside, blood can flow out of the tube body 1 through the connector, and then a red blood cell layer or a platelet layer and the like are discharged, the disturbance on layered blood is small, a suction tube does not need to be used for extending into the liquid level to suck the platelet layer, the platelet layer is prevented from being dispersed by tiny shaking in the suction operation, and the number of extracted platelets is increased; the side wall of the suction pipe is prevented from being stained with platelets to reduce the number of the extracted platelets, and the platelet enrichment rate is further improved; and vortex impact on the platelet layer can be avoided when the suction tube sucks the platelet, so that the extraction concentration of the platelets can be further improved.
The concentration of the obtained platelets is 4-9 times of the physiological concentration by using the centrifugal device to carry out secondary centrifugal operation, the concentration of neutrophils, eosinophils and basophils is 1.3-1.5 times of the physiological concentration, the platelet enrichment rate is 70-75%, the platelet enrichment rate in the related technology is 55-65%, and the enrichment effect is greatly improved.
The centrifugal device for extracting the platelet-rich plasma has the advantages of simple structure, convenience in operation, high platelet enrichment rate, reduction in platelet loss and capability of effectively enriching three types of granular cells and platelet plasma.
In some embodiments, as shown in fig. 1 and 2, the first connector 4 and the second connector 6 have the same structure and are luer-cone locking cones.
The first connector 4 and the second connector 6 are luer conical locking conical heads with 6% conical degree, so that the sealing performance is good, and blood is prevented from being polluted.
In some embodiments, as shown in fig. 1, 2 and 3, the first pipe cover 5 and the second pipe cover 7 have the same structure, and include a cover top 5-1 and a cover body 5-2, which are connected, and the bottom of the cover body 5-2 is provided with a first connecting structure 5-3, and the first connecting structure 5-3 is used for being detachably connected with the first connector 4 or the second connector 6.
The cover top 5-1 is arranged to facilitate operation by a person, the cover body 5-2 is used for closing the first connector 4 or the second connector 6, the first connecting structure 5-3 is a screw thread, which is not limited in particular and can be connected with the first connector 4 or the second connector 6 in a threaded manner, and the first connector 4 or the second connector 6 can be completely opened, half opened or completely closed by rotating the cover top 5-1.
In some embodiments, as shown in fig. 4, the centrifugation device for extracting platelet rich plasma further comprises: the interior of the bidirectional connector 8 is of a hollow structure, one end of the bidirectional connector is provided with a second connecting structure 8-1, the other end of the bidirectional connector is provided with a third connecting structure 8-2, the second connecting structure 8-1 is used for being detachably connected with the first connector 4 or the second connector 6, and the third connecting structure 8-2 is used for being detachably connected with the injector.
The second connecting structure 8-1 is, for example, a screw thread, etc., the third connecting structure 8-2 is, for example, a snap, etc., and is not limited in particular, the second connecting structure 8-1 is used for connecting the first connector 4 or the second connector 6, and the third connecting structure 8-2 is used for connecting a syringe, so that negative pressure can be formed in the tube 1 by drawing the syringe, liquid can flow out of the tube 1 from the connectors quickly, and platelet-rich plasma can be extracted conveniently.
In some embodiments, as shown in fig. 1 and 2, the tube body 1 is a cylinder with a diameter of 20-35mm and a height of 35-100mm, and the centrifugal effect is better.
In some embodiments, as shown in fig. 1 and 2, the first step structure 2 includes a first step 2-1, and the first step 2-1 is a cylinder having a diameter equal to that of the pipe body 1.
The first step structure 2 is arranged downwards during the first centrifugal operation, the red blood cell layer at the bottom is discharged through the first connector 4 after centrifugation, and the diameter of the first step 2-1 is set to be equal to that of the tube body 1, so that red blood cells can be conveniently settled on the first step 2-1 and can be conveniently collected.
In some embodiments, as shown in fig. 2, the first step 2-1 is provided with a second step 2-2 connected thereto, and the second step 2-2 is a cylinder with a diameter of 6-16mm and a height of 20-50mm.
The second step 2-2 is arranged on the first step 2-1, so that erythrocyte sedimentation is further facilitated, vortex is prevented from being formed during centrifugation, a platelet layer is dispersed, the diameter of the second step 2-2 is 6-16mm, the height of the second step is 20-50mm, impact on the platelet layer is small, and erythrocyte sedimentation effect is better.
In some embodiments, as shown in fig. 1 and 2, the second step structure 3 includes a third step 3-1 and a fourth step 3-2 which are communicated with each other, the third step 3-1 and the fourth step 3-2 are both cylindrical, the diameter of the third step 3-1 is equal to that of the pipe body 1, the diameter of the fourth step 3-2 is 6-18mm, and the height is 30-60mm.
The second step structure 3 is downwards arranged during the second centrifugal operation, three granulocytes and a platelet layer at the bottom are discharged through the second connector 6 after the centrifugation, the third step 3-1 and the fourth step 3-2 are arranged, the cells are conveniently layered by means of density gradient, the platelets and the granulocytes are easily settled on the steps, the blood is not easy to form vortex to impact the platelet layer, the enrichment and extraction of platelet-rich plasma are facilitated, the diameter of the fourth step 3-2 is 6-18mm, the height of the fourth step 3-2 is 30-60mm, and the platelet enrichment effect is better.
In some embodiments, as shown in fig. 2, the fifth step 3-3 is connected to the fourth step 3-2, and the diameter of the fifth step 3-3 is 6-11mm, which is more effective for enriching platelets.
The fifth step 3-3 is further convenient for layering of cells by means of density gradient, platelets and granulocytes are easy to settle on the steps, blood is not easy to form vortex and impact a platelet layer, enrichment and extraction of platelet-rich plasma are facilitated, experimental results show that the platelet-rich concentration extracted by the third step structure 3 is better, the content of three kinds of granulocytes is higher, and the platelet enrichment rate is as high as 75%.
In some embodiments of the present application, a system for extracting platelet rich plasma is provided, comprising a centrifugation device as described in any of the embodiments above for extracting platelet rich plasma.
The centrifugal system for extracting the platelet-rich plasma is convenient to operate and high in platelet enrichment rate.
Some embodiments of the present application provide methods of operating a secondary centrifuge, comprising:
s1, blood sampling preassembly: a needle head is arranged on a first syringe, and anticoagulant sodium citrate is pre-filled in the first syringe for venous blood collection; opening the first joint 4 of the centrifugal device for extracting the platelet-rich plasma, extending the first syringe needle into the first joint 4, pushing the first syringe to inject the blood into the tube body 1, taking out the first syringe needle, and closing the first joint 4.
S2, first centrifugation: the first joint 4 of the centrifuge is placed downwards into the centrifuge, and after balancing, the centrifuge is centrifuged.
S3, separating a red blood cell layer: taking out the centrifuged centrifugation device, opening the first joint 4, connecting the first joint 4 and the second injector by using the bidirectional joint 8, half-opening the second joint 6, drawing the second injector to absorb the erythrocyte layer at the bottom of the centrifugation device, closing the second joint 6, detaching the second injector and the bidirectional joint 8, and closing the first joint 4.
S4, centrifuging for the second time: the second joint 6 of the centrifuge is placed downwards into the centrifuge and after balancing, the centrifuge is centrifuged.
S5, separating a platelet-rich plasma layer: taking out the centrifuged centrifugal device, opening the second joint 6, connecting the second joint 6 with the third syringe by using the bidirectional joint 8, half opening the first joint 4, drawing the third syringe to absorb the platelet-rich plasma layer at the bottom of the centrifugal device, closing the first joint 4, detaching the third syringe and the bidirectional joint 8, and closing the second joint 6.
The first centrifugation is used for separating the red blood cell layer, and the second centrifugation is used for separating the platelet rich plasma layer from the platelet poor supernatant; half open first joint 4 or second and connect 6, make body 1 and external intercommunication, the blood in the body 1 can slowly flow out body 1, prevents that blood from flowing out fast, improves the syringe and absorbs the precision, further improves rich platelet plasma concentration, rich platelet plasma layer includes three kinds of granulocyte layers and/or platelet layer.
The method has the advantages of convenient operation, high platelet enrichment rate, reduced platelet loss, and effective enrichment of three kinds of granular cells and platelet plasma.
Those of ordinary skill in the art will understand that: the discussion of any embodiment above is meant to be exemplary only, and is not intended to intimate that the scope of the disclosure, including the claims, is limited to these examples; within the context of the present application, features from the above embodiments or from different embodiments may also be combined, steps may be implemented in any order, and there are many other variations of the different aspects of the embodiments of the present application as described above, which are not provided in detail for the sake of brevity.
Additionally, where details are set forth in order to describe example embodiments of the present application, it will be apparent to one skilled in the art that the present embodiments may be practiced without, or with variation of, these details. Accordingly, the description is to be regarded as illustrative instead of restrictive.
While the present application has been described in conjunction with embodiments thereof, many alternatives, modifications, and variations thereof will be apparent to those skilled in the art in light of the foregoing description.
The present embodiments are intended to embrace all such alternatives, modifications and variances which fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalents, improvements, and the like that may be made without departing from the spirit or scope of the embodiments of the present application are intended to be included within the scope of the claims.
Claims (10)
1. A centrifuge device for extracting platelet rich plasma, comprising:
the blood transfusion tube comprises a tube body, a first tube and a second tube, wherein the tube body is of a hollow structure and used for containing blood, the top of the tube body is of a first step structure, and the bottom of the tube body is of a second step structure;
the first joint is arranged on the first step structure in a penetrating manner, the top of the first joint is detachably connected with a first pipe cover, and the first pipe cover is used for opening or closing the first joint;
the second joint is arranged on the second step structure in a penetrating mode, the bottom of the second joint is detachably connected with a second pipe cover, and the second pipe cover is used for opening or closing the second joint.
2. The centrifuge device for extracting platelet rich plasma of claim 1, wherein the first connector and the second connector are identical in structure and are luer cone locking cones.
3. The centrifuge apparatus for extracting platelet-rich plasma according to claim 2, wherein the first tube cap and the second tube cap have the same structure, and comprise a cap top and a cap body which are connected, and the bottom of the cap body is provided with a first connecting structure for detachably connecting with the first connector or the second connector.
4. The centrifuge device for extracting platelet rich plasma according to claim 2, further comprising:
the two-way joint, inside is hollow structure, and one end is equipped with second connection structure, and the other end is equipped with third connection structure, second connection structure be used for with first joint or the connection can be dismantled to the second joint, third connection structure is used for dismantling with the syringe and is connected.
5. The centrifuge device for extracting platelet rich plasma of claim 1, wherein the tube is cylindrical with a diameter of 20-35mm and a height of 35-100mm.
6. The centrifuge device for extracting platelet rich plasma of claim 5, wherein the first step structure comprises a first step that is a cylinder with a diameter equal to the diameter of the tube.
7. The centrifuge device for extracting platelet-rich plasma according to claim 6, wherein the first step is provided with a second step which is connected with the first step, the second step is a cylinder, the diameter of the second step is 6-16mm, and the height of the second step is 20-50mm.
8. The centrifuge device for extracting platelet-rich plasma according to claim 5, wherein the second step structure comprises a third step and a fourth step which are communicated, the third step and the fourth step are both cylindrical, the diameter of the third step is equal to that of the tube, the diameter of the fourth step is 6-18mm, and the height of the fourth step is 30-60mm.
9. The centrifuge device for extracting platelet-rich plasma according to claim 8, wherein the fourth step is provided with a communicated fifth step, and the diameter of the fifth step is 6-11mm.
10. A system for extracting platelet rich plasma comprising a centrifuge device according to any of claims 1 to 9 for extracting platelet rich plasma.
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