CN218126192U - Automatic control system for long-term continuous smoke exposure of multichannel small animals - Google Patents

Abstract

The utility model provides an automatic control system for long-term continuous smoke exposure of multi-channel small animals, which comprises a smoke mixing box body, at least one feeding box, a control box, an exhaust emission device and a smoke generator; the control box is connected and communicated with the lower part of the smoke mixing box body; the feeding box is communicated with the control box through a pipeline; the waste gas discharge device is arranged in the smoke mixing box body; the smoke generator is connected with the control box. The utility model discloses an automatic control system that continuous smog of multichannel toy long term exposes overcomes current smog and exposes defect that parallel experiment can not be done to toy intermittent type nature contamination in the system, passageway singleness, can truly effectively simulate the actual conditions, the error of the experimental result that significantly reduces and data.

Description

Automatic control system for long-term continuous smoke exposure of multichannel small animals

Technical Field

The utility model relates to a toy smog exposes microenvironment preparation system field especially relates to an automatic control system that continuous smog of multichannel toy long term exposes.

Background

At present, respiratory diseases (mainly including asthma, COPD and the like) caused by factors such as increasingly serious atmospheric environmental pollution (especially PM 2.5), smoking and population aging become one of ten death causes of death of the current Chinese population. However, the exact etiology and pathogenesis of these diseases are not completely understood, and the research on this aspect is not open to the corresponding animal model of respiratory diseases. In addition, gas exposure animal experiments are also needed for researches on the toxicology of harmful gases in the environment, the physiological function of high and low oxygen, and the like. Therefore, small animal smoke exposure device systems are an indispensable technical means for this research.

At present, the smoke exposure technology for the small animals comprises an oral-nasal exposure system and a whole-body exposure system, and the small animals are exposed in the smoke environment in a specific box body or a cavity. These two types of devices have common deficiencies: the small animals can not eat in the smoke exposure bin, and leave the smoke environment when eating, and can not be continuously infected with the poison for a long time, so the smoke exposure mode belongs to an intermittent smoke exposure mode. Therefore, such contamination experiments cannot well simulate real actual environmental conditions, such as altitude reaction, and certain deviation occurs in experimental results and data. In addition, the oral-nasal exposure system and the whole-body exposure system have respective characteristics, because the oral-nasal exposure system adopts a tower type cavity structure, only the mouth and the nose of a mouse are exposed in a smog environment, the air fluidity in an exposure cabin is good, however, the smoke concentration value of the mode cannot be accurately monitored, the number of parallel experimental animals is limited, and the equipment cost is relatively expensive; the general exposure system, i.e. the most common smoking box or box, has poor ventilation and gas quality, is not easy to control and monitor precise parameters, and has the defects of poor repeatability, lack of standardized procedures and metering, and the like. Therefore, in order to truly and effectively simulate a respiratory inhalation microenvironment (long-time continuous exposure, accurate index control and monitorable numerical value) and establish a respiratory disease damage model with high stability and repeatability, a microenvironment system which can be accurately and automatically controlled by the small animal through long-time continuous exposure and smoke is designed, and the method has very important significance for development and research of respiratory disease pathogenesis and therapeutic drugs.

Disclosure of Invention

Not enough to above-mentioned prior art, the utility model provides an automatic control system that continuous smog was exposed in multichannel toy long term overcomes current smog and exposes the defect that the toy intermittent type nature is contaminated in the system, can truly effectively simulate actual conditions, the error of the experimental result that significantly reduces and data.

In order to achieve the purpose, the utility model provides an automatic control system for long-term continuous smoke exposure of multi-channel small animals, which comprises a smoke mixing box body, at least one feeding box, a control box, a waste gas discharge device and a smoke generator; the control box is connected and communicated with the lower part of the smoke mixing box body; the feeding box is communicated with the control box through a pipeline; the waste gas discharge device is arranged in the smoke mixing box body; the smoke generator is connected with the control box.

Preferably, a plurality of sensors are arranged in the smoke mixing box body, and the sensors adopt the combination of any one of an oxygen sensor, a smoke sensor, a PM2.5 sensor and a medicine atomization aerosol sensor; an air mixing fan is further arranged in the smoke mixing box body.

Preferably, an oxygen inlet, a specific smoke inlet and a specific gas inlet are arranged on the bottom surface of the smoke mixing box body; the bottom surface of the smoke mixing box body is provided with a plurality of groups of first gas exchange ports.

Preferably, the breeding box comprises a cage box, a trough grid net, a drinking water bottle and a cage cover; the trough grid net is fixed in the cage box, and the drinking bottles are placed on the trough grid net; the cage cover sealing cover is arranged on the cage box and connected with the cage box through a buckle.

Preferably, the feeding box is formed with a set of second gas exchange ports.

Preferably, the control box comprises a measurement and control circuit, and a power supply, a power switch, a plurality of pump switches, an oxygen solenoid valve gas valve, a specific gas solenoid valve gas valve and a plurality of groups of third gas exchange ports which are connected with the measurement and control circuit; the oxygen electromagnetic valve is connected with the oxygen input port through a pipeline; the special gas electromagnetic valve is connected with the special gas input port through a pipeline.

Preferably, the control box further comprises a smoke input pump, and the smoke input pump is connected with the smoke generator and is connected with the specific smoke input port through a pipeline; the control box also comprises a plurality of gas circulating pumps; the second gas exchange port and the third gas exchange port are connected through a silicone tube; the smoke input pump and the gas circulating pump are connected with the measurement and control circuit.

Preferably, a plurality of carbon dioxide adsorption devices are further arranged in the control box.

Preferably, the measurement and control circuit comprises a main control chip board, a display screen and a plurality of parameter setting buttons; the display screen and the parameter setting button are arranged on the outer wall of the control box and connected with the main control chip board; the main control chip board is also connected with the sensor.

The utility model discloses owing to adopted above technical scheme, make it have following beneficial effect:

(1) The utility model discloses a toy smog exposes experimental apparatus technical field, has had more experimental apparatus at present, but the common weak point of this type of device all is to expose and lacks animal diet device in the environment, and the animal can only intermittent type nature smog expose, can not simulate long-term continuous life at the actual conditions of specific atmospheric environment well. The utility model discloses a smog mixes the box and follows sealed coupling of raising case intercommunication, and in the continuous longer time exposes in specific smog gaseous environment, avoids the animal frequently to take out smog environment because of diet, causes the deviation of experimental result, simulates actual living environment really.

(2) The existing whole-body type fumigation box and mouth-nose type exposure device are generally single channels, the number of animals in parallel experiments at each time is limited, and only single smoke concentration environment exposure can be carried out at each time, so that concentration gradient experiments cannot be completed simultaneously. Therefore, the parallel experiments have the defects of batch-to-batch difference and multiple experiments with multiple concentrations. The utility model discloses a multiunit gas exchange mouth in the smog mixes the box can connect a plurality of sealed raising casees and realize that the parallel animal experiment of multiunit goes on simultaneously. In addition, by setting different smoke concentrations of each sealed feeding box, the master control program can adjust the air valve of the electromagnetic valve, and the calculated air flow is respectively injected into different sealed feeding boxes, so that different gradient control is realized.

(3) The utility model discloses smog mixing arrangement passes through the silicone tube pipeline with exposing sealed raising box among the technical system and links to each other, parts relatively each other, has reduced vibrations and noises such as control box pump, valve effectively, reduces the influence to the toy, has guaranteed experimental result authenticity and repeatability.

(4) The existing mouth-nose type exposure device has the defects that the numerical value of smoke concentration cannot be accurately monitored, and the smoking box or smoking box device has poor air permeability and gas quality, is difficult to control and monitor precise parameters, has poor repeatability, lacks of standardized procedures and metering and the like. The utility model realizes the suction of microenvironment in the sealed feeding box according to the set limit value in real time through the control system, and has the characteristics of accurate and controllable smoke concentration in the exposed environment and high stability and repeatability of the prepared animal model; and, the utility model discloses be equipped with oxygen single channel control system, the animal damage that microenvironment hypoxemia arouses when can effectively adjust smog and pour into, the deviation that the outer damage of animal experiment condition arouses is avoided.

(5) The utility model has the characteristics of the usage is wide, the customization degree is high, can be according to the experiment purpose difference, install different sensing module, the experiment is easy and simple to handle and humanized, avoids respiratory disease research in-process because of disease, damage factor, treatment factor difference and need be equipped with different instrument and equipment, effectively saves instrument and equipment experiment cost.

Drawings

Fig. 1 is a schematic structural diagram of an automatic control system for long-term continuous smoke exposure of a multi-channel small animal according to an embodiment of the present invention;

fig. 2 is a schematic structural view of a smoke mixing box according to an embodiment of the present invention;

fig. 3 is a schematic structural diagram of a control box according to an embodiment of the present invention;

fig. 4 is a schematic structural view of the feeding box of the embodiment of the present invention.

Detailed Description

The following description of the preferred embodiment of the present invention will be given in detail with reference to the accompanying drawings, fig. 1 to 4, so as to better understand the functions and features of the present invention.

Referring to fig. 1 to 4, the automatic control system for long-term continuous smoke exposure of multi-channel small animals according to the embodiment of the present invention comprises a smoke mixing box 1, at least one feeding box 3, a control box 2, an exhaust emission device 4 and a smoke generator 5; the control box 2 is connected and communicated with the lower part of the smoke mixing box body 1; the feeding box 3 is communicated with the control box 2 through a pipeline; the exhaust emission device 4 is arranged on the smoke mixing box body 1; the smoke generator 5 is connected with the control box 2.

A plurality of sensors are arranged in the smoke mixing box body 1, and the sensors adopt the combination of any one of an oxygen sensor 11, a smoke sensor 12, a PM2.5 sensor 13 and a medicine atomization gas sensor 15; an air mixing fan 16 is also arranged in the smoke mixing box body 1. The top of the smoke mixing box body 1 forms an exhaust air outlet 14 connected with the exhaust air discharging device 4.

The bottom surface of the smoke mixing box body 1 is provided with an oxygen input port 17, a specific smoke input port 18 and a specific gas input port 19; the bottom surface of the smoke mixing box body 1 is provided with a plurality of groups of first gas exchange ports 110.

The breeding box 3 comprises a cage box 31, a trough grid net 32, a drinking bottle 33 and a cage cover 34; the crib grid net 32 is fixed in the cage box 31, and the drinking bottles 33 are placed on the crib grid net 32; the cage cover 34 is hermetically covered on the cage 31 and connected with the cage 31 through a buckle 35.

The feeding box 3 is formed with a set of second gas exchange ports 36.

The control box 2 comprises a measurement and control circuit, a power supply 21 connected with the measurement and control circuit, a power supply switch 22, a plurality of pump switches, an oxygen solenoid valve air valve 23, a specific gas solenoid valve air valve 24 and a plurality of groups of third gas exchange ports 25; the oxygen solenoid valve air valve 23 is connected with the oxygen input port 17 through a pipeline; the special gas solenoid valve 24 is connected to the special gas input port 19 through a pipe.

The control box 2 also comprises a smoke input pump 26, and the smoke input pump 26 is connected with the smoke generator 5 and is connected with the specific smoke input port 18 through a pipeline; the control box 2 also comprises a plurality of gas circulation pumps 27; the second gas exchange port 36 and the third gas exchange port 25 are connected through a silicone tube; the smoke input pump 26 and the gas circulating pump 27 are connected with the measurement and control circuit.

The smoke input pump 26 is composed of an oil-resistant diaphragm gas pump, and has the characteristics of low noise and rapid and stable gas injection in the smoke and atomization gas input process, so that the experimental result is prevented from generating errors due to unstable microenvironment caused by frightening animals by noise and unstable gas injection.

A plurality of carbon dioxide adsorption devices 28 are also arranged in the control box 2. The carbon dioxide adsorption device 28 is filled with an adsorbent such as soda lime. For removing carbon dioxide from the circulating gas between the fume mixing box and the sealed rearing box 3.

The measurement and control circuit comprises a main control chip board 29, a display screen 210 and a plurality of parameter setting buttons 211; the display screen 210 and the parameter setting button 211 are arranged on the outer wall of the control box 2 and connected with the main control chip board 29; the main control chip board 29 is also connected with a sensor. The control program of the measurement and control circuit is compiled based on the principle of a single chip microcomputer, real-time analysis and feedback of data monitored by the sensor are realized, and the smoke concentration of the smoke mixing box is controlled in real time according to a set limit value; and can be externally connected with a computer to realize the output and storage of monitoring data.

Oxygen solenoid valve gas valve 23 and specific gas solenoid valve gas valve 24: an oxygen bottle and a specific gas bottle are externally connected through a silicone tube, so that the control system can automatically open an electromagnetic valve gas valve to input oxygen and specific gas into the flue gas mixing box body when the monitoring value of a sensor is lower than a set limit value according to the concentration limit values of the oxygen and the specific gas in the set exposure environment; otherwise, when the monitoring value is higher than the set point limit value, the control system automatically closes the electromagnetic valve, so that the experimental deviation of the animal caused by hypoxia and instability of specific gas is avoided.

The exhaust gas discharge device in the smoke mixing box consists of an electromagnetic valve, an active carbon adsorbent and a pipeline, the smoke mixing box can be controlled by opening and closing the valve, and the valve is closed during exposure, so that the concentration of gas in the box body is prevented from being changed rapidly; when smog, gas were injected, perhaps after the experiment ended, the valve was opened, and it is outdoor to discharge after the active carbon adsorption treatment with waste gas, prevents harmful gas to experimenter's health harm.

The utility model discloses automatic control system that continuous smog of multichannel toy long term exposes, guard point and innovation point are as follows:

1. the utility model discloses the system device comprises smog mixing box, sealed raising case 3 and control box 2, wherein seals raising case 3 and provides smog and exposes the environment, separately adopts the silicone tube coupling to communicate with each other with the smog mixing box, and control box 2 maintains the stability of sealed raising case 3 smog environment, accomplishes that the toy living environment is the contamination environment promptly to can carry out the animal experiment of contamination in succession for a long time, simulate the true condition:

2. the sealed rearing box 3 is fastened by a cage box 31, a trough grid net 32 and a cage cover 34 to form a sealed rearing environment, and performs gas communication with the smoke mixing box through a third gas exchange port 25 on the side wall of the cage box 31 to form a smoke exposure environment with stable circulation.

3. The smoke mixing box is provided with a sensor, an exogenous smoke/fog/air injection port and one or more groups of first gas exchange ports 110 which are in circulation with the sealed feeding box 3, and simultaneously, a plurality of groups of parallel animal experiments are completed;

4. the control box 2 is composed of an electromagnetic valve air valve, an air inlet/outlet pump, a circulating pump and a control main board, can automatically adjust the smoke/fog/air concentration of the smoke mixing box, and realizes multi-level and multi-gradient animal experiments under the smoke environment with different concentrations of small animals through the opening and closing time of the electromagnetic valve air valve and the circulating pump.

5. The utility model discloses the system provides a circulating gas purification and filtration device between sealed raising box 3 and the mixing box, can effectual elimination toy expired carbon dioxide and moisture, guarantees the purity and the stability of gas composition.

For example: preparation of mouse model exposed to different hypoxic concentrations

Hypoxic environments cause a range of symptoms of high altitude reactions, including acute and chronic symptoms. Meanwhile, the oxygen partial pressure at different altitudes is different, so that hypoxia with different degrees is caused, and the phenomena of headache (headache is the most common symptom), insomnia, anorexia, fatigue, dyspnea and the like are caused. The chronic hypoxia state can cause general physiological and pathological symptoms such as heart failure, lung injury, liver cirrhosis, digestive tract hemorrhage and the like. Therefore, the establishment of mouse models with different hypoxia concentrations is helpful for the research of hypoxic-altitude reaction. This example establishes a hypoxic mouse model at three concentrations of 13.6% (corresponding to 3500 m), 18% (corresponding to 1800 m), and 21% (normal oxygen partial pressure).

1. The oxygen cylinder and the nitrogen cylinder are respectively connected with the oxygen interface of the smoke mixing box and other specific gas interfaces, and the switch of the gas cylinder is turned on.

2. The three sealed feeding boxes 3 are respectively filled with padding, sufficient feed and drinking water (which meet a week), and the air inlet and the air outlet are connected with the circulating channels #1 (13.6%), #2 (18%), #3 (21%) of the smoke mixing box through silica gel pipelines.

3. The power supply 21 of the control box 2 is turned on, three oxygen concentration limits corresponding to three channels are set on the operation screen, an upper limit (+ 0.5%) and a lower limit (-0.5%) are set for each concentration, and the average value is three concentration values.

4. After setting, 5 mice per group were placed in the sealed rearing box 3, and the cage lid 34 was fastened and the exhaust system was closed.

5. And clicking the operation button to automatically control the subsequent operation steps by the control system. Specifically, a nitrogen electromagnetic valve is automatically opened to inject nitrogen into the smoke mixing box, the diluted oxygen is only a minimum set value (13.6%), and the #1 channel circulating pump is closed after the gas exchange between the #1 channel circulating pump and the 13.6% sealed feeding box 3 is finished. And the rear system opens an oxygen solenoid valve air valve 23 to inject oxygen to 18%, and closes the circulating pump of the channel #2 after the gas exchange between the channel #2 and the 18% sealed feeding box 3 is completed. By analogy, the smoke mixing box is maintained at the oxygen concentration of 21% after completing the gas exchange of the channel # 3. The subsequent control system can measure and calculate the consumption condition of oxygen in each feeding box 3 according to the number of mice, the breathing rate and the volume of the feeding boxes 3, and when the consumption condition is lower than the lower limit value set by concentration, the flow of the mixed gas injected in sequence through the circulating pump is calculated.

6. The mice are raised in different low-oxygen environments for 7 days without changing padding and adding diet and the like.

7. After the model is made to the animal, open exhaust system, arrange the residual flue gas in the microenvironment box through the pipeline completely, take out the animal, close power 21.

The multi-channel automatic control system for long-time continuous smoke exposure of the small animals, provided by the embodiment of the utility model, can prepare a smoke microenvironment with multiple channels, has stable concentration, is accurate and controllable, and can effectively control experimental errors among batches; the prepared hypoxia disease model has high success rate, stable model and good repeatability.

The present invention has been described in detail with reference to the embodiments shown in the drawings, and those skilled in the art can make various modifications to the present invention based on the above description. Therefore, certain details of the embodiments should not be construed as limitations of the invention, which are intended to be covered by the following claims.

Claims (9)

1. An automatic control system for long-term continuous smoke exposure of multi-channel small animals is characterized by comprising a smoke mixing box body, at least one feeding box, a control box, an exhaust emission device and a smoke generator; the control box is connected and communicated with the lower part of the smoke mixing box body; the feeding box is communicated with the control box through a pipeline; the waste gas discharge device is arranged on the smoke mixing box body; the smoke generator is connected with the control box.

2. The automatic control system for long-term continuous smoke exposure of small animals according to claim 1, wherein a plurality of sensors are arranged in the smoke mixing box, and the sensors adopt any combination of an oxygen sensor, a smoke sensor, a PM2.5 sensor and a drug atomization aerosol sensor; an air mixing fan is further arranged in the smoke mixing box body.

3. The automatic control system for long-term continuous smoke exposure of small multi-channel animals as claimed in claim 2, wherein the bottom surface of the smoke mixing box body is provided with an oxygen inlet, a specific smoke inlet and a specific gas inlet; the bottom surface of the smoke mixing box body is provided with a plurality of groups of first gas exchange ports.

4. An automatic control system for long term continuous smoke exposure of multi-channel small animals as claimed in claim 3, wherein said feeding box comprises a cage box, a trough grid net, a drinking water bottle and a cage cover; the trough grid net is fixed in the cage box, and the drinking bottles are placed on the trough grid net; the cage cover sealing cover is arranged on the cage box and connected with the cage box through a buckle.

5. An automated multi-channel system for long term continuous smoke exposure of small animals as claimed in claim 4, wherein said enclosure is formed with a set of second gas exchange ports.

6. The system as claimed in claim 5, wherein the control box comprises a measurement and control circuit, a power supply, a power switch, a plurality of pump switches, an oxygen solenoid valve, a specific gas solenoid valve and a plurality of groups of third gas exchange ports; the oxygen electromagnetic valve is connected with the oxygen input port through a pipeline; the special gas electromagnetic valve is connected with the special gas input port through a pipeline.

7. The automatic control system for long-term continuous smoke exposure of small multi-channel animals as claimed in claim 6, wherein said control box further comprises a smoke input pump, said smoke input pump is connected with said smoke generator and connected with said specific smoke input port through a pipeline; the control box also comprises a plurality of gas circulating pumps; the second gas exchange port and the third gas exchange port are connected through a silicone tube; the smoke input pump and the gas circulating pump are connected with the measurement and control circuit.

8. The automatic control system for long-term continuous smoke exposure of small multi-channel animals as claimed in claim 7, wherein a plurality of carbon dioxide adsorption devices are further arranged in the control box.

9. The automatic control system for long-term continuous smoke exposure of multi-channel small animals as claimed in claim 8, wherein the measurement and control circuit comprises a main control chip board, a display screen and a plurality of parameter setting buttons; the display screen and the parameter setting button are arranged on the outer wall of the control box and connected with the main control chip board; the main control chip board is also connected with the sensor.

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