CN109674557A - A kind of Animal Inhalation Toxicity Exposure experiment exposure case and exposing device - Google Patents
A kind of Animal Inhalation Toxicity Exposure experiment exposure case and exposing device Download PDFInfo
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- CN109674557A CN109674557A CN201910052549.8A CN201910052549A CN109674557A CN 109674557 A CN109674557 A CN 109674557A CN 201910052549 A CN201910052549 A CN 201910052549A CN 109674557 A CN109674557 A CN 109674557A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
Abstract
The invention proposes a kind of Animal Inhalation Toxicity Exposures to test with case and exposing device is exposed, and belongs to toxicological experiment equipment technical field.The cabinet is divided by animal exposed region and guiding region by active plugboard in this exposure case, is respectively equipped with air inlet and fog inlet on the wall box of guiding region side；The top that active plugboard is located at guiding region side is fixed with the deflector of layering setting, it is used to form S type runner and is mixed sufficiently being diluted by the current-carrying air of the air inlet and the drying of fog inlet entrance with water mist, mixed gas is passed through the animal exposed region through multiple ventholes that active plugboard lower part is uniformly arranged；Multiple gas vents are respectively equipped on the wall box of animal exposed region side.This exposing device includes the Animal Inhalation Toxicity Exposure experiment exposure case, air pump, vaporizer and Hygrothermograph.The configuration of the present invention is simple, cost is relatively low, can test the true effect of simulated environment water mist exposure, and can carry out multiple groups experiment simultaneously.
Description
Technical field
The invention belongs to toxicological experiment equipment technical field, in particular to a kind of Animal Inhalation Toxicity Exposure experiment exposure case
And exposing device.
Background technique
It can be by eating, sucking and three kinds of approach of skin are exposed to human body, wherein sucking by the pollutant of medium of water
Since exposed amount is smaller, exposure chamber is complicated for exposure, studies less.However, since shortage of water resources problem is serious, at sewage
Become inexorable trend as reclaimed water reuse after reason.Recycled water utilization rate rises year by year, be widely used in agricultural irrigation, carwash,
Flush the toilet, landscape water and river supplement etc. number of ways.It is molten that different degrees of gas can occur during above-mentioned utilization for recycled water
Gelatinization, there may be health risks after being sucked by human body.Existing research shows the pollutant (such as endotoxin) in water through aerosol
Can generate health hazard after change to human body, the fever symptoms such as famous " have a bath and burn " and " humidifier burning " all with it is aerosolized by
Contaminant water is related.Therefore, it is necessary to it is aerosolized to recycled water progress, suck experimental animal, to study and evaluate recycled water
Health hazard to experimental animal.
Existing Animal Inhalation Toxicity Exposure is broadly divided into two classes with exposing device:
One kind is commercial integration product, collects computerized control system, air treatment system and exposure functional unit in one
In large-scale cabinet body, exposure parameter (temperature, air pressure, aerosol concentration etc.) can be adjusted automatically, but its price is very expensive
(every price hundreds of thousands yuan), volume are excessive, occupy and laboratory larger space and are not easy to move, be only capable of every time using
A kind of water sample is exposed, and must consumption some time detergent line and cabinet body after exposing；Also, exposure experiment often needs solid every time
Determine parameter, computer is precisely controlled parameter function and had not only occupied cabinet body space, but also keeps product entirety price high, but necessity is little.
Another kind of exposing device is simple cabinet, lacks water conservancy diversion region, the water sample of atomization is directly in the form of high concentration water mist
Still it is in the droplet state of white haze shape into animal exposed region, is stained be licked in meeting on animal skin by animal and licks entrance, increase drink
Vaporous water exposure in food route of exposure, with actual conditions differs greatly, and humidity is excessive will increase animal sense of discomfort.
Summary of the invention
It is an object of the invention to overcome the shortcoming of prior art, a kind of Animal Inhalation Toxicity Exposure experiment exposure is provided
Case and exposing device, operation of the present invention is easy, it is low in cost, occupy little space, move flexibly, and experiment effect is preferable.
To achieve the goals above, the present invention adopts the following technical scheme:
A kind of Animal Inhalation Toxicity Exposure experiment exposure case proposed by the present invention, including lighttight cabinet, the top of the box
Case lid equipped with light transmission, and be sealed between top of the box and case lid by seal washer；It is characterized in that, in the cabinet
The cabinet is divided into animal exposed region and guiding region by the active plugboard placed vertically, the wall box positioned at guiding region side
On be respectively equipped with air inlet and fog inlet；The top that the active plugboard is located at guiding region side is fixed with the water conservancy diversion of layering setting
Plate is used to form S type runner and will sufficiently be mixed by the current-carrying air and water mist of the air inlet and the drying of fog inlet entrance
It closes, mixed gas is passed through the animal exposed region through multiple ventholes that active plugboard lower part is uniformly arranged；Positioned at described
Multiple gas vents are respectively equipped on the wall box of animal exposed region side.
Further, which is divided into multiple sub-districts by movable partition plate by the animal exposed region
Domain, for accommodating different groups of experimental animal；Multiple ventholes are uniformly opened up on the movable partition plate.
The present invention also proposes a kind of exposing device being made of the experiment of above-mentioned Animal Inhalation Toxicity Exposure with exposure case, and feature exists
In, including Animal Inhalation Toxicity Exposure experiment exposure case, air pump, vaporizer and Hygrothermograph；The air pump is logical
The dried chimney filter filled with desiccant and filter membrane is crossed to be connected to Animal Inhalation Toxicity Exposure experiment with the air inlet of exposure case, and
The gas flow of the air pump is controlled by spinner flowmeter；The vaporizer and Animal Inhalation Toxicity Exposure experiment are used
The fog inlet connection of exposure case；The Hygrothermograph is fixed on the inside of case lid of the Animal Inhalation Toxicity Exposure experiment with exposure case.
Feature of the present invention and the utility model has the advantages that
1, water conservancy diversion region is set, and the water mist and current-carrying air that sample generates enter back into animal exposure after being sufficiently mixed uniformly
Humidity size in exposed region is adjusted by adjusting current-carrying air mass flow in area.
2, it can be exposed in the state of humidity unsaturation, real simulation, which works, exposes scene, and makes to animal
At sense of discomfort it is lower.
3, compared with fully automatic integral device, volume is smaller, and cost is lower, and structure is simple, convenient for cleaning.
4, multiple and different groups of animals can be exposed simultaneously, saves experimental period.
Detailed description of the invention
Fig. 1 is experiment of the embodiment of the present invention exposure integrally-built decomposition diagram of case.
Fig. 2 is the structural schematic diagram of cabinet in Fig. 1.
Fig. 3 is the structural schematic diagram of active plugboard in Fig. 1.
Specific embodiment
To technical solution of the present invention, detailed description are as follows with reference to the accompanying drawings and embodiments:
It is a kind of overall structure diagram of Animal Inhalation Toxicity Exposure experiment exposure case of the embodiment of the present invention referring to Fig. 1,
Including opaque cabinet 1, which is equipped with light transmission case lid 2, and between 1 top of cabinet and case lid 2 by seal washer 3 into
Row seals, and is fixed with Hygrothermograph on 2 inner wall of case lid；Cabinet 1 is divided by the active plugboard 4 placed vertically in cabinet 1 and is
Object exposed region I and guiding region II are respectively equipped with air inlet 11 and fog inlet 12, air inlet on 1 side wall of cabinet of II side of guiding region
Mouth 11 filled with desiccant (bibulous desiccant can be used, such as molecular sieve or discoloration silica gel) and filter membrane by (such as using
High efficiency particulate air filter HEPA filter membrane) dried chimney filter and cabinet 1 outside air pump (drying tube and air pump in figure not
Illustrate) connection, for being passed through dry current-carrying air into the guiding region of cabinet 1 II, air enters guiding region through drying tube
Spinner flowmeter is first passed through before II, so as to real-time monitoring and adjusts throughput size, the atomizer outside fog inlet 12 and cabinet 1
(atomizer does not illustrate in figure) connection, for being passed through water mist into the guiding region of cabinet 1 II；Active plugboard 4, which is located at, leads
The top of stream II side of area is fixed with the deflector of layering setting, is used to form S type runner and water mist and dry current-carrying is empty
Gas is sufficiently mixed, and multiple ventholes 43 that mixed gas is uniformly arranged through 4 lower part of active plugboard are passed through animal exposed region I；
Multiple gas vents 13 are respectively equipped on 1 side wall of cabinet of I side of animal exposed region.
The specific implementation and function of each building block are respectively described below in the present embodiment:
Referring to fig. 2, cabinet 1 is made as this experiment main structure of exposure case of opaque plastics, can be real
Animal is tested light protected environment is provided, reduces human interference.When box sizes are arranged, need in conjunction with maximum experimental animal quantity and
Influence of the humidity to overall humidity in cabinet caused by maximum experimental animal breathing, the relative humidity that usual animal itself introduces is not
It should be more than 50%, to aqueous vapor exposure there are space, the present embodiment uses rectangular box 1, interior path length 840mm, width 360mm, height
230mm (can accommodate up to adult mice 10, rat 3-4 only).
Case lid 2 is made of the organic glass of light transmission, is convenient for the case where observing animal in case at any time, and in 2 inner wall of case lid
On be fixed with Hygrothermograph (not illustrating in figure), temperature and humidity variation can be observed in cabinet 1 in real time to calculate water in case
Vapor content.
Seal washer 3 uses one layer of silicone gasket, for guaranteeing the good sealing effect of entire cabinet, cabinet 1, gasket
It is fixed between circle 3 and case lid 2 by wing screw.
Referring to fig. 2, Fig. 3, active plugboard 4 are mounted on cabinet 1 by the cutting being fixed on two opposite inner sidewalls of cabinet 1
It is interior, cabinet 1 is divided into animal exposed region I and guiding region II.4 top of active plugboard of the present embodiment is equipped with leading for two layers of level
Flowing plate 41 and 42,4 lower part of active plugboard are equipped with venthole 43, and the air inlet 11 and fog inlet 12 on 1 side wall of cabinet are close to upper layer
The same side of deflector is arranged, and is respectively positioned on the top of upper layer deflector 41, the other side of upper layer deflector 41 and 1 side wall of cabinet
Between it is spaced, pass through for current-carrying air；The side of lower layer's deflector 42 and 1 inner sidewall of cabinet are close to lower layer's deflector 42
Middle part open up deflector hole；Dry air and water mist passes through air inlet 11 respectively and fog inlet 12 is passed through in guiding region II, and
It is sufficiently mixed in upper and lower layer deflector motion process, finally the deflector hole inflow activity in the middle part of lower layer's deflector is inserted
The venthole 43 of 4 lower part of plate, hence into animal exposed region I.Well-mixed air and water mist can make to enter animal exposed region I
Humidity keep stablizing, and make the relative humidity difference of different location in behaviour area within 5%.
Animal exposed region in cabinet 1 is real for the ease of carrying out exposure to multiple groups animal simultaneously for accommodating experimental animal
It tests, animal exposed region can be divided by movable partition plate 5 for multiple animal activity subregions, open up 9 on movable partition plate 5
The venthole 51 of × 13 column of row, in order to avoid the head of experimental animal is caught in venthole 51, venthole 51 be should not be too large, this implementation
The internal diameter of each venthole 51 of example is 5mm (situation for considering suckling mouse exposure).It, can be by movable partition plate 5 when exposed Rats
It moves and removes.Multiple gas vents are opened respectively on each side wall of animal exposed region, gas in case are discharged continually and steadily with flexible modulation, and can
Emission-control equipment or draught cupboard are connect, experiment confirms that opening symmetrical gas vent can guarantee that humidity is uniform in animal exposed region.This
In embodiment, in animal exposed region, 3 gas vents are symmetrically opened up on the opposite two sidewalls of cabinet 1, are opened on another side wall of cabinet 1
If 4 gas vents.
When carrying out exposure experiment using this exposure case, need to cooperate air pump, dried chimney filter, spinner flowmeter, doctor
It is used with atomizer and Hygrothermograph, and can simultaneously multiple exposed casees be carried out with the exposure of different water samples.The gas outlet of air pump
It is connect with dried chimney filter, the air after dry filter enters guiding region II through the air inlet 11 on 1 side wall of cabinet, can pass through tune
Spinner flowmeter is saved to adjust air mass flow；Atomizer fog outlet is connect with the fog inlet 12 on 1 side wall of cabinet.Hygrothermograph can
It is fixed on case lid 2, facilitates through case lid 2 and check that temperature and humidity changes at any time.Before exposure, each component is first connected, hygroscopicity is used
Strong desiccant (such as molecular sieve or discoloration silica gel) filling drying tube, and air pump is opened, so that dry air is entered cabinet, current-carrying
The flow velocity of air is adjusted to 5-20L/min by spinner flowmeter, at this time it can be seen that the intracorporal humidity of case continues to decline, the present invention
By current-carrying gas velocity adjustment humidity, current-carrying gas velocity is big, then equilibrium humidity is small, ties up after humidity declines to a certain extent
Hold constant, openable vaporizer switch at this time.In guiding region II, water mist can be sufficiently mixed and dilute with dry air,
Water mist becomes aqueous vapor after water conservancy diversion, enters animal exposed region I (i.e. exposed region) by the venthole 43 of deflector lower part, cabinet 1
Interior humidity persistently rises, and can rise to 30-50%, and the rate of climb first quick and back slow, finally basically reaches balance.It is warm and humid by recording
The variation of degree, animal generate humidity substantially constant, wherein the relative humidity differential of animal exposed region quadrangle can be controlled in 5% with
It is interior to calculate the reconditioning of animal whithin a period of time.The exposure in the form of vaporous water of this exposure case meets most of environment
Exposed feature, if the exposure in the form of liquid water (water mist), first is that since water mist diffusion is slow, it is difficult to ensure that water mist uniformly divides
Cloth；Second is that supersaturated water mist can be congealed on animal skin droplet licked through animal lick entrance can not block it is sudden and violent except sucking exposure
Dew approach；Third is that humidity is excessively high to cause the serious sense of discomfort of animal, and it is not excluded for other unknown influences.
In addition, a exposed case of N (N is positive integer, according to experiment it needs to be determined that) can be also together in parallel, it is more to carry out simultaneously
Group exposure experiment.At this point, each cabinet 1 shares an air pump, pass through the corresponding exposure case of a spinner flowmeter control respectively
Current-carrying air mass flow, each exposed case provides water mist by atomizer respectively, and the tail gas generated in experimentation is unified to be received
Collection processing.
The present invention also proposes a kind of Animal Inhalation Toxicity Exposure experiment exposing device being made of above-mentioned exposed case, the device packet
Include above-mentioned exposed case, air pump, dried chimney filter, spinner flowmeter, vaporizer and Hygrothermograph.
Each electronic device according to the present invention is commercial product.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: it still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features；
And these are modified or replaceed, technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution spirit and
Range.
Claims (5)
1. a kind of Animal Inhalation Toxicity Exposure experiment exposure case, including lighttight cabinet, the top of the box are equipped with the case lid of light transmission,
And it is sealed between top of the box and case lid by seal washer；It is characterized in that, by placing vertically in the cabinet
The cabinet is divided into animal exposed region and guiding region by active plugboard, is respectively equipped with air inlet on the wall box of guiding region side
Mouth and fog inlet；The top that the active plugboard is located at guiding region side is fixed with the deflector of layering setting, is used to form S type
Runner will be simultaneously sufficiently mixed by the current-carrying air of the air inlet and the drying of fog inlet entrance with water mist, mixed gas
The animal exposed region is passed through through multiple ventholes that active plugboard lower part is uniformly arranged；Positioned at animal exposed region side
Multiple gas vents are respectively equipped on wall box.
2. Animal Inhalation Toxicity Exposure experiment exposure case according to claim 1, which is characterized in that the deflector is by level
The upper layer deflector and lower layer's deflector of setting form；Air inlet and fog inlet on the wall box is close to upper layer deflector
The same side setting, and be respectively positioned on the top of upper layer deflector, between the other side and wall box of upper layer deflector there are
Every passing through for current-carrying air；The side of lower layer's deflector and box inside wall in the middle part of lower layer's deflector close to offering
Deflector hole.
3. Animal Inhalation Toxicity Exposure experiment exposure case according to claim 1, which is characterized in that the animal exposed region is logical
It crosses movable partition plate and the animal exposed region is divided into multiple subregions, for accommodating different groups of experimental animal；The work
Multiple ventholes are uniformly opened up on dynamic separation plate.
4. Animal Inhalation Toxicity Exposure experiment exposure case according to claim 1, which is characterized in that the animal exposed region
Interior, the gas vent on the opposite two sidewalls of cabinet is arranged symmetrically.
5. a kind of experiment of the Animal Inhalation Toxicity Exposure described in any one of Claims 1 to 4 is filled with the exposure that exposure case is constituted
It sets, which is characterized in that test including the Animal Inhalation Toxicity Exposure with exposure case, air pump, vaporizer and Hygrothermograph；Institute
State air pump by dried chimney filter filled with desiccant and filter membrane and Animal Inhalation Toxicity Exposure experiment exposure case into
Port is connected to, and the gas flow of the air pump is controlled by spinner flowmeter；The vaporizer and the animal inhale
Enter the fog inlet connection of exposure experiment exposure case；The Hygrothermograph is fixed on the Animal Inhalation Toxicity Exposure experiment exposure case
Case lid on the inside of.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910052549.8A CN109674557A (en) | 2019-01-21 | 2019-01-21 | A kind of Animal Inhalation Toxicity Exposure experiment exposure case and exposing device |
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| CN201910052549.8A CN109674557A (en) | 2019-01-21 | 2019-01-21 | A kind of Animal Inhalation Toxicity Exposure experiment exposure case and exposing device |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111896345A (en) * | 2020-07-30 | 2020-11-06 | 上海化工研究院有限公司 | Device for uniformly coating medicine film on inner wall of container in toxicity contact experiment |
| CN112730738A (en) * | 2020-12-07 | 2021-04-30 | 中国科学院深圳先进技术研究院 | Evaluation device and evaluation method for reward and addiction of atomized matter |
| CN113984962A (en) * | 2021-10-08 | 2022-01-28 | 山东大学 | Inhalation particulate matter respiration detection device and method |
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