CN217695073U - Table-board liquid nitrogen device for freezing and thawing embryos - Google Patents

Abstract

The utility model discloses a table-board liquid nitrogen device for freezing and unfreezing embryos, which comprises a tank body, wherein a frame body is integrally formed or detachably erected on one side in the tank body; the horizontal section of the frame body is crescent, and the sectional area of the frame body gradually increases from the upper end to the lower end to form an inclined plane; a platform protrudes below the inclined plane; a straw hole site is integrally formed or machined on the inclined plane and the platform; the straw hole is provided with a long semi-cylinder cambered surface on the inclined surface and a jack arranged on the platform and positioned below the long semi-cylinder cambered surface; the upper end of the jack is open and is connected with the arc surface of the long semi-cylinder; the straw hole site is used for assisting in inserting and matching the operation carrying rod and the straw sleeve; when the embryo transfer is met, the butt joint of the straw carrying rod and the straw sleeve, the batch check of the frozen embryos, the search of the thawed embryos and the batch check can be assisted, and the timeliness, the simplicity and the reliability of the embryo freezing and thawing operation are improved.

Description

Table-board liquid nitrogen device for freezing and thawing embryos

Technical Field

The utility model relates to an auxiliary reproduction field especially relates to a mesa liquid nitrogen device that is used for embryo freezing to unfreeze.

Background

In Vitro Fertilization and Embryo Transfer (IVF-ET; commonly known as "tube baby" Technology) is the core Technology of human Assisted Reproductive Technology (ART) and is the main technical means for treating infertility. In the artificial assisted reproduction treatment process, a plurality of ova can be obtained at one time through a controlled superovulation technology, a plurality of embryos can be formed after in vitro fertilization, after 1-2 high-quality embryos are transplanted, the embryos or the ova which reach the standard need to be frozen and stored, the frozen embryos or the ova can be unfrozen in the next period and then transplanted or fertilized, and therefore the treatment efficiency and the treatment effect of ART are guaranteed to the maximum extent. Embryo freezing is a routine and important procedure in assisted reproduction, and currently over 50% of "test-tube babies" are born by transplantation of pregnancies after thawing frozen embryos. At present, the embryo is frozen mainly by adopting a vitrification freezing mode, and the freezing process and the preservation of the embryo are carried out in liquid nitrogen. Under the liquid nitrogen environment of minus 196 ℃, the metabolism of the embryo is basically stopped, so that the embryo can be preserved in a large liquid nitrogen preservation tank for a long time. When embryos are frozen in batches, a table-top liquid nitrogen device is needed, particularly, after the embryo freezing operation is completed on an IVF workstation console, a freezing carrying rod containing the embryos needs to be placed in liquid nitrogen of the table-top liquid nitrogen device for sleeving and temporary storage, and finally, the table-top liquid nitrogen device is used for transferring the frozen embryo straws into a liquid nitrogen storage tank for long-term storage. When the embryo is unfrozen, the embryo straw in the liquid nitrogen storage tank is transferred to the operation table by using the table liquid nitrogen device.

The embryo freezing method comprises the following steps that a special freezing straw is needed to be used for freezing embryos, the freezing straw generally comprises a carrying rod and a straw sleeve, and the front end (head) of the carrying rod is a thin concave surface or a plane and is used for loading embryos; a straw sleeve can be sleeved on the carrier rod for sealing so as to protect embryos on the carrier rod. When the embryo is frozen, the straw sleeve is firstly drawn out and placed in the table-board tank body for standby, when the processed embryo is positioned at the front end of the carrying rod, the processed embryo is quickly put into the table-board tank body, then the carrying rod is inserted into the corresponding straw sleeve in liquid nitrogen and tightly sleeved, the front end of the carrying rod filled with the embryo is always kept below the liquid level of the liquid nitrogen in the sleeving process, finally, the sleeved straw is firstly placed in the straw frame, and then the straw frame is placed in the corresponding position in the liquid nitrogen storage tank. Before thawing, taking out a specific wheat tube frame from a liquid nitrogen storage tank and putting the specific wheat tube frame into a table-board tank body, checking information, and quickly extracting the specific wheat tube in the wheat tube frame by using large metal tweezers and putting the specific wheat tube into liquid nitrogen. Several embryo straws needing to be thawed in one batch are usually placed in the table top tank body at the same time, and large metal tweezers are needed to find out the embryo straws needing to be thawed when the embryo straws are thawed.

At present, a table-board tank body special for embryo freezing and thawing is not available, such as a heat preservation pail or a foam box is often adopted, however, the heat preservation pail or the foam box is only used as a table-board transfer liquid nitrogen container and does not have the function of assisting the freezing and thawing operation, particularly assisting a freezing sleeve (butting an embryo carrying rod and the sleeve), batch checking of frozen embryos, searching of thawed embryos and batch checking and the like.

SUMMERY OF THE UTILITY MODEL

To the not enough of present lack special mesa liquid nitrogen transfer device, the utility model provides a mesa liquid nitrogen device for embryo freezing unfreezes when satisfying the embryo transfer, can also assist and carry pole and straw sleeve butt joint, freezing embryo's batch to check, unfreeze embryo's searching and batch to check, improved the ageing, simplicity and reliability of embryo freezing operation of unfreezing.

The technical scheme of the utility model as follows:

a table-board liquid nitrogen device for freezing and unfreezing embryos comprises a tank body, wherein a frame body is integrally formed or detachably erected on one side in the tank body; the horizontal section of the frame body is crescent, and the sectional area of the frame body gradually increases from the upper end to the lower end to form an inclined plane; a platform protrudes below the inclined plane; a straw hole site is integrally formed or machined on the inclined plane and the platform; the straw hole is provided with a long semi-cylinder cambered surface on the inclined surface and a jack arranged on the platform and positioned below the long semi-cylinder cambered surface; the upper end of the jack is open and is connected with the arc surface of the long semi-cylinder; the straw hole site is used for assisting the insertion and the matching of the operation carrying rod and the straw sleeve.

And a placing groove for placing the inserted and matched straw is further formed in the platform on one side or two sides of the jack.

And the position of the placing groove is higher than that of the jack.

Wherein, the end of the placing groove close to the jack is an open end.

Wherein the inclination angle of the inclined plane is 70-80 degrees.

The arc surface of the long semi-cylinder is a semi-arc surface, and the radius of the arc surface is the same as that of the jack.

Wherein, the jar body sets up the handle.

The utility model discloses following beneficial effect has:

1. the utility model discloses when satisfying the embryo transfer, can also assist the butt joint of straw and wheat pipe box, the batch of freezing embryo is checked, the search of embryo that unfreezes is checked and is checked in batches, has improved ageing, simplicity and convenience and the reliability of embryo freezing unfreezing operation.

2. The utility model discloses a support body has the inclined plane, has also ensured the slope of straw hole site, has further ensured the straw cover lower part and has inserted and establish in the jack, and well upper portion natural slope is relied on long semi-cylinder cambered surface, not only can effectively ensure the position and the gesture of straw cover stable, suitable operator will carry the pole advantage of taking advantage of, insert the straw cover fast, accurately along long semi-cylinder cambered surface moreover to reduce the operation degree of difficulty, improved operating efficiency and reliability.

3. The utility model discloses the straw hole site sets up to the lower part and encircles spacing hollow cylinder, and well upper portion guide and half spacing long half cylinder cambered surface, its straw cover hypomere inserts in hollow cylinder by spacingly when the straw hole site is arranged in to the straw, and carry the pole tail section then on the liquid nitrogen liquid level to not only can realize that the fixed of straw cover is put with simple and convenient getting, and convenient the embryo identification information of checking on the straw carries the pole tail section in batches, can compromise promptly well supplementary freezing thawing operation and make the work of checking of batch information again.

4. The utility model is provided with the placing grooves at the two sides of the position of the jack and at the slightly higher positions, which can be used for arranging and placing the inserted straws; firstly, the arrangement sequence of the straws in the placing grooves is the sequence of the straw inserting and matching operation, and the disorder of the sequence can be avoided; thirdly, straws placed in rows can be conveniently and clearly conditioned for checking by operators; and fourthly, only one straw hole site is needed, and the position of the hole site is fixed, so that an operator can conveniently improve the operation efficiency and the operation accuracy by utilizing muscle memory.

Drawings

FIG. 1 is a schematic vertical sectional view of the present invention taken through a jack;

FIG. 2 is a schematic vertical sectional view of the placement groove of the present invention;

FIG. 3 is a schematic view of the utility model after placing the straw;

FIG. 4 is a schematic cross-sectional view of the center line of the tank body facing the direction of the frame body;

fig. 5 is the utility model discloses just to the jar body central line profile sketch map of support body direction.

The reference numbers in the figures denote:

1. a tank body; 2. a frame body; 21. a bevel; 22. a platform; 23. a placement groove; 3. the position of a straw hole; 31. a jack; 32. a long semi-cylindrical arc surface; 4. a straw sleeve; 5. a carrier bar; 6. a handle.

Detailed Description

The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

Referring to fig. 1 to 5, a table-board liquid nitrogen device for embryo freezing and thawing comprises a tank body 1, wherein a frame body 2 is integrally formed or detachably erected on one side in the tank body 1; the horizontal section of the frame body 2 is crescent, and the sectional area is gradually increased from the upper end to the lower end to form an inclined plane 21; a platform 22 protrudes below the inclined plane 21; a straw hole position 3 is integrally formed or machined on the inclined surface 21 and the platform 22; the straw hole site 3 is provided with a long semi-cylinder cambered surface 32 on the inclined surface 21 and a jack 31 which is arranged on the platform 22 and is positioned below the long semi-cylinder cambered surface 32; the upper end of the jack 31 is open and is connected with the long semi-cylinder cambered surface 32; the straw hole site 3 is used for assisting the insertion of the operation carrying rod 5 and the straw sleeve 4.

Furthermore, a placing groove 23 for placing the inserted straws is also arranged on the platform 22 at one side or two sides of the inserting hole 31.

Further, the placement groove 23 is positioned higher than the insertion hole 31.

Further, an end of the placement groove 23 adjacent to the insertion hole 31 is an open end.

Further, the long semi-cylinder arc surface 32 is a semi-arc surface, and the radius of the arc surface is the same as that of the insertion hole 31.

Further, the tank body 1 is provided with a handle 6.

The concrete structure and parameters are as follows:

1. the inclined plane 21 inclines to one side from top to bottom, the inclination angle is 75 degrees, the inclination of the straw hole site 3 is guaranteed through the inclination of the inclined plane 21, the lower part of the straw sleeve 4 is further guaranteed to be inserted into the jack 31, the middle upper part naturally inclines to lean against the long semi-cylindrical cambered surface 32, the position and the posture of the straw sleeve 4 can be effectively guaranteed to be stable, and an operator can conveniently, quickly and accurately insert the carrying rod 5 into the straw sleeve 4 along the long semi-cylindrical cambered surface 32, so that the operation difficulty is reduced, and the operation efficiency and the reliability are improved.

2. The straw hole site 3 is used for butt joint of the straw carrying rod 5 and the straw sleeve 4, the whole length is 15cm, the diameter is 3mm, the straw carrying rod is divided into an upper section and a lower section, the lower section is an open cylinder, namely a hole 31, and the length is 1cm; the lower open cylinder extends upwards to form a long semi-cylinder cambered surface 32 of the upper section, and the length of the long semi-cylinder cambered surface 32 is 14cm; the straw hole site 3 sets up to the lower part and encircles spacing jack 31, and the semi-spacing long semi-cylinder cambered surface 32 of well upper portion to not only can realize the fixed of straw cover 4, can also make things convenient for getting of straw cover 4 to can conveniently look over the label on the 5 afterbody of carrier bar, thereby make things convenient for operation process.

3. The frame body 2 is an integral body which is L-shaped and comprises an inclined plane 21 and a platform 22, and the whole frame body 2 and the tank body 1 are integrally formed or the whole frame body 2 is integrally formed and is detachably arranged with the tank body 1; the horizontal section of the frame body 2 is crescent, which can be engaged with the cylindrical tank body 1 and save the space in the tank body 1.

The operation method comprises the following steps:

(1) When the embryo is frozen, liquid nitrogen is filled into the tank body 1 and is nearly full, the top end of the upper section long semi-cylinder cambered surface 32 of the straw hole site 3 must be exposed out of the liquid nitrogen surface, the exposed length is not less than 1cm, and thus the straw hole site 3 can be easily seen by naked eyes. Putting straw sleeves 4 corresponding to a batch of embryos to be frozen into the tank body 1, pressing the straws to be frozen to be below the liquid level of liquid nitrogen by using a clamp, filling the straw sleeves 4 with the liquid nitrogen, clamping the straw sleeves 4 by using a large metal clamp, and quickly putting the straws into the straw hole positions 3. The straw sleeve 4 filled with liquid nitrogen slides down along the long semi-cylinder cambered surface 32 of the straw hole site 3 under the action of gravity until the lower section of the straw hole site 3, and the straw sleeve 4 is fixed due to the fact that the lower section of the straw hole site 3 has a complete cylinder side surface. After the frozen embryos are spotted on the head parts, namely the front ends, of the carrying rods 5, the handheld carrying rods 5 are quickly placed into the tank body 1, so that the head parts of the carrying rods 5 containing the embryos are instantly placed below the liquid nitrogen liquid level, the top ends of the upper sections of the straw hole positions 3 are exposed out of the liquid nitrogen liquid level, the carrying rods 5 can be easily and lightly inserted into the corresponding hole positions along the tracks of the long semi-cylindrical cambered surfaces 32 by hands until the carrying rods are inserted into the preset straw sleeves 4 and are slightly and forcibly inserted, then the inserted straws are lifted by a small height, and after the carrying rods are higher than the placing grooves 23, the straws are translated to one side of the placing grooves 23, so that the straws are supported on the placing grooves 23; this is repeated until all straws completed mating are arranged on the placement groove 23. After all embryos are frozen, the frozen embryos are transferred to the corresponding position of a liquid nitrogen storage tank by using the tank body 1. And to conventional pail, owing to do not have and be similar to the utility model discloses such maintenance structure of straw hole site 3 and standing groove 23, after freezing a pipe embryo, the straw is deposited in the pail bottom at random with regard to a pipe, after a batch freezing operation, can't carry out simple and convenient efficient once more and concentrate and check.

(2) When the embryo is unfrozen, liquid nitrogen is filled into the mesa tank body 1 to be nearly full, all embryos to be unfrozen are taken out from corresponding positions in the liquid nitrogen storage tank, are quickly placed into the tank body 1, and are sequentially arranged in the placing groove 23 according to the arranged unfreezing sequence. After straws subjected to thawing operation in the same batch are arranged on the placing groove 23, corresponding information and sequence can be easily and clearly checked and carefully confirmed in a centralized manner, and in addition, straws to be thawed need to be searched for by repeatedly transferring the straws to the bottom of the liquid nitrogen lifting barrel from the conventional process are not needed during thawing.

At present, a table top tank body 1 specially used for freezing and unfreezing embryos does not exist, and a common heat-preservation lifting barrel does not have the function of assisting the freezing and unfreezing operation. Aiming at the defect, the embryo transfer device can assist the butt joint of the embryo carrying rod 5 and the sleeve 4, the batch check of frozen embryos, the search and batch check of thawed embryos while meeting the requirement of embryo transfer, and improves the timeliness, simplicity and reliability of embryo freezing and thawing operation.

The above only is the embodiment of the present invention, not limiting the patent scope of the present invention, all the equivalent structures or equivalent processes that are used in the specification and the attached drawings or directly or indirectly applied to other related technical fields are included in the patent protection scope of the present invention.

Claims (7)

1. A mesa liquid nitrogen device for embryo freeze-thaw which characterized in that: comprises a tank body (1), wherein a frame body (2) is integrally formed or detachably erected on one side in the tank body (1); the horizontal section of the frame body (2) is crescent, and the sectional area is gradually increased from the upper end to the lower end to form an inclined plane (21); a platform (22) protrudes below the inclined plane (21); a straw hole site (3) is integrally formed or machined on the inclined plane (21) and the platform (22); the straw hole site (3) is provided with a long semi-cylinder cambered surface (32) on the inclined surface (21) and a jack (31) which is arranged on the platform (22) and is positioned below the long semi-cylinder cambered surface (32); the upper end of the jack (31) is open and is connected with the long semi-cylinder cambered surface (32); the straw hole site (3) is used for assisting the insertion and the matching of the operation carrying rod (5) and the straw sleeve (4).

2. The mesa liquid nitrogen apparatus for frozen thawing of embryos of claim 1, wherein: and a placing groove (23) for placing the inserted straws is also formed in the platform (22) on one side or two sides of the insertion hole (31).

3. The mesa liquid nitrogen device for frozen thawing of embryos of claim 2, wherein: the position of the placing groove (23) is higher than that of the insertion hole (31).

4. The mesa liquid nitrogen device for frozen thawing of embryos of claim 3, wherein: one end of the placing groove (23) close to the insertion hole (31) is an open end.

5. The mesa liquid nitrogen apparatus for frozen thawing of embryos of claim 1, wherein: the inclination angle of the inclined plane (21) is 70-80 degrees.

6. A mesa liquid nitrogen apparatus for embryo freeze thawing according to any of claims 1 to 5, characterized in that: the long semi-cylinder cambered surface (32) is a semi-circular cambered surface, and the radius of the cambered surface is the same as that of the jack (31).

7. A mesa liquid nitrogen apparatus for embryo freeze thawing according to any of claims 1 to 5, characterized in that: the tank body (1) is provided with a handle (6).

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