CN213169058U - Vitrification thawing liquid nitrogen box - Google Patents
Vitrification thawing liquid nitrogen box Download PDFInfo
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- CN213169058U CN213169058U CN202021493792.8U CN202021493792U CN213169058U CN 213169058 U CN213169058 U CN 213169058U CN 202021493792 U CN202021493792 U CN 202021493792U CN 213169058 U CN213169058 U CN 213169058U
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Abstract
The utility model discloses a vitrification unfreezing liquid nitrogen box, which is matched with an auxiliary reproductive freezing and storing device comprising a freezing and storing bracket, and comprises a heat preservation box body and a bracket base device arranged in the inner cavity of the heat preservation box body; the top surface of the heat preservation box body is provided with an opening; the bracket base device comprises a bracket group mechanism and a base which are both made of metal heat conduction materials, and also comprises a slide glass cap clamp mechanism; the bracket group mechanism comprises an upper bracket, a lower bracket and a supporting surface which are fixedly arranged on the base, and the slide cap clamp mechanism comprises a supporting column and an openable clamping piece component which are all made of metal heat conduction materials, and also comprises a clamping rod with a clamping handle. Adopt the utility model discloses a liquid nitrogen box is unfrozen in vitrification can improve the efficiency that frozen gamete or embryo vitrification unfreeze and prevent to obscure.
Description
Technical Field
The utility model relates to a heat preservation box, concretely relates to vitrification liquid nitrogen box that unfreezes.
Background
The emergence of human assisted reproduction technology brings good news to infertile couples, and cryopreservation of gametes and embryos is an indispensable part of human assisted reproduction technology. Cryopreservation techniques have been improved and innovated over the past two decades and sperm, ova and embryos at different times have now been cryopreserved. The vitrification freezing technology is a freezing preservation technology developed in recent years, and has the advantages that (1) no ice crystal is formed, and the damage to the freezing and thawing process of cells or tissues is small; secondly, the operation is simple, the time consumption is short, and the cost is low; and freeze thawing effect is good, survival rate is high. At present, the traditional slow freezing technology is widely accepted by the medical field and gradually replaced, and the traditional slow freezing technology becomes the best choice of the freezing technology. The technique has been successfully applied to cryopreservation of immature oocytes, mature oocytes, cleavage stage embryos, blastocysts and the like. The auxiliary reproductive cryopreservation device comprises an openable cryopreservation bracket, wherein a freezing carrying rod, a carrying sheet and a carrying sheet cap are arranged in an inner cavity of the cryopreservation bracket; the head of the freezing carrying rod is provided with a slide (a storage point for gametes or embryos), and the slide is sleeved in a slide cap.
When the gametes or embryos stored in the vitrification frozen manner are thawed, the auxiliary reproductive frozen storage device stored in a liquid nitrogen tank is transferred to a heat preservation box filled with liquid nitrogen by using forceps and then conveyed to an embryo freezing chamber for thawing, and a slide cap, a slide (filled with gametes or embryos) and a freezing carrying rod are taken out from a frozen storage bracket and lean against the inner wall of the heat preservation box for standby; when thawing, holding the freezing carrying rod with one hand, holding the forceps with the other hand to remove the slide carrying cap on the freezing carrying rod, and quickly transferring the slide to the thawing solution to finish the thawing process of gametes or embryos.
At present, no heat preservation box special for vitrification thawing generally uses a foam box, but when different gametes or embryos are thawed simultaneously, confusion is easily caused; the freezing carrying rod is not specially placed, and the freezing carrying rod is easy to topple when leaning against the inner wall of the heat preservation box; nor is there a means to remove the slide cap. Accordingly, there is a need for improvements in the art.
SUMMERY OF THE UTILITY MODEL
The to-be-solved problem of the utility model is to provide a convenient to use's vitrification liquid nitrogen box that unfreezes.
In order to solve the technical problem, the utility model provides a vitrification unfreezing liquid nitrogen box which is matched with an auxiliary reproductive freezing and storing device comprising a freezing and storing bracket, wherein the vitrification unfreezing liquid nitrogen box comprises a heat preservation box body and a bracket base device arranged in the inner cavity of the heat preservation box body; the top surface of the heat preservation box body is provided with an opening;
the bracket base device comprises a bracket group mechanism and a base which are both made of metal heat conduction materials, and also comprises a slide glass cap clamp mechanism;
the base is attached to the bottom surface of the inner cavity of the heat preservation box body;
the bracket group mechanism comprises an upper bracket, a lower bracket and a supporting surface which are fixedly arranged on the base, the upper bracket, the lower bracket and the supporting surface are sequentially arranged, the central lines of the upper bracket, the lower bracket and the supporting surface are overlapped, and the tops of the upper bracket and the lower bracket are respectively provided with a groove; the distance between the upper bracket groove and the supporting surface is less than the length of the freezing storage bracket;
the slide cap clamp mechanism comprises a support column and an openable clamping piece assembly, the support column and the openable clamping piece assembly are both made of metal heat conduction materials, the slide cap clamp mechanism further comprises a clamping rod with a clamping handle, the support column is fixedly connected with the base, the clamping piece assembly is arranged on the support column, and the clamping rod is connected with the clamping piece assembly.
As the utility model discloses a vitrification unfreezes improvement of liquid nitrogen box:
the side wall of the inner cavity of the heat preservation box body is respectively provided with a liquid nitrogen lowest horizontal line and a liquid nitrogen highest horizontal line;
the bottom of the groove of the lower bracket is not higher than the lowest level of liquid nitrogen, and the bottom of the groove of the upper bracket is higher than the highest level of liquid nitrogen;
the height of the clamping piece assembly is not higher than the lowest liquid nitrogen level.
As the utility model discloses a vitrification unfreezes further improvement of liquid nitrogen box:
a partition plate is arranged in the middle of the base, and a set of bracket set mechanisms is respectively arranged on two sides of the partition plate;
the center lines of the upper bracket, the lower bracket and the supporting surface are parallel to the clapboard.
As the utility model discloses a vitrification unfreezes further improvement of liquid nitrogen box:
the clamping rod is Z-shaped, and the clamping handle is positioned on the periphery of the heat preservation box body.
As the utility model discloses a vitrification unfreezes further improvement of liquid nitrogen box:
a vertical freezing loading rod placing pore channel is arranged on the base; the top height of the freezing loading rod placing hole is not higher than the lowest level of liquid nitrogen, and the freezing loading rod placing hole is tightly attached to the upper support.
As the utility model discloses a vitrification unfreezes further improvement of liquid nitrogen box:
the top of the upper bracket is provided with a label.
As the utility model discloses a vitrification unfreezes further improvement of liquid nitrogen box:
the clamping piece assembly is in a cylindrical shell shape consisting of two semi-cylindrical shell-shaped clamping pieces, and the axis of the clamping piece assembly and the base form an included angle of 45 degrees.
As the utility model discloses a vitrification unfreezes further improvement of liquid nitrogen box: the outer side wall of the heat preservation box body is provided with a forceps groove for placing forceps.
As the utility model discloses a vitrification unfreezes further improvement of liquid nitrogen box: the bottom surface of the inner cavity of the heat preservation box body is provided with a fixing column, and the base is provided with a through hole matched with the fixing column.
The utility model has the following technical advantages:
1. the bracket group mechanism is used for placing the freezing storage bracket so that the freezing storage bracket cannot drift in liquid nitrogen.
2. The top of the upper bracket is provided with a label marked with a number, so that the freezing storage bracket arranged on the bracket group mechanism can be distinguished, and the freezing carrying rod arranged in the freezing carrying rod arranging hole can also be distinguished.
3. The slide glass on the freezing carrying rod and the slide glass cap are inserted into the freezing carrying rod placing hole, so that the slide glass (carrying gametes or embryos) can be positioned in liquid nitrogen, and the phenomenon of toppling over can not occur.
4. When the glass is unfrozen, the glass-carrying cap clamp mechanism can be used for conveniently and quickly removing the glass-carrying cap connected to the freezing carrying rod.
5. Adopt the utility model discloses a liquid nitrogen box is unfrozen in vitrification can improve the efficiency that frozen gamete or embryo vitrification unfreeze and prevent to obscure.
Drawings
The following describes the present invention in further detail with reference to the accompanying drawings.
FIG. 1 is a schematic structural view of a vitrification thawing liquid nitrogen box of the present invention;
fig. 2 is a schematic structural diagram of the stand base device 1 in fig. 1.
Detailed Description
The invention will be further described with reference to specific examples, but the scope of the invention is not limited thereto.
As shown in fig. 1-2, the vitrified thawing liquid nitrogen box comprises a support base device 1 and a heat preservation box body 2, wherein the support base device 1 comprises a base 14, and the base 14 is of a rectangular flat plate structure.
The heat preservation box body 2 is a hollow cuboid box which is made of heat preservation materials and has no top surface (namely, an opening on the top surface), the length and the width of the inner cavity of the heat preservation box body 2 are equal to the length and the width of the base 14, so that the inner cavity of the heat preservation box body 2 can be just placed into the support base device 1, and the base 14 is attached to the bottom surface of the inner cavity of the heat preservation box body 2. The base 14 is provided with a through hole 141 for fixing at the two ends of a diagonal line respectively, correspondingly, a fixing column 23 matched with the through hole 141 is arranged at the two ends of a diagonal line at the bottom surface of the inner cavity of the heat preservation box body 2 respectively, and when the fixing column 23 is sleeved in the through hole 141, the whole bracket base device 1 and the heat preservation box body 2 are positioned and fixed; the side wall of the inner cavity of the heat preservation box body 2 is provided with a liquid nitrogen minimum horizontal line 21 and a liquid nitrogen maximum horizontal line 22, the outer side wall of the heat preservation box body 2 is provided with a forceps groove 24, and forceps 25 are placed in the forceps groove 24.
The support base device 1 comprises a support group mechanism, a slide cap clamp mechanism, a partition plate 12 and a base 14, wherein the whole support group mechanism, the partition plate 12 and the base 14 are made of metal heat conduction materials.
The bracket set mechanism, the slide glass cap clamp mechanism and the partition plate 12 are all fixedly arranged on the base 14; a partition plate 12 is transversely arranged in the middle of the base 14, and the partition plate 12 is parallel to the long edge of the base 14. A set of bracket group mechanism for placing the freezing storage bracket is respectively arranged at two sides of the partition plate 12.
Each set of support group mechanism comprises an upper support 111, a lower support 112 and a supporting surface 113 which are fixedly arranged on the base 14, the upper support 111, the lower support 112 and the supporting surface 113 are sequentially arranged, the central lines of the upper support 111, the lower support 112 and the supporting surface 113 are coincided and are parallel to the partition board 12, and the tops of the upper support 111 and the lower support 112 are respectively provided with a groove; the spacing between the grooves of the upper shelf 111 and the support surface 113 is less than the length of the freezer storage shelves so that the freezer storage shelves can rest on the grooves of the upper shelf 111 and the grooves of the lower shelf 112 with the end of the freezer rod carrying the slide adjacent the lower shelf 112 and the support surface 113 is configured to abut the end of the freezer storage shelves so that the slide is positioned between the lower shelf 112 and the support surface 113.
The matching of the grooves of the upper bracket 111 and the grooves of the lower bracket 112 prevents the transverse displacement (sliding) of the refrigeration storage bracket, and the support surface 113 prevents the longitudinal displacement (sliding) of the refrigeration storage bracket.
The bottom of the groove of the lower bracket 112 is not higher than the liquid nitrogen minimum horizontal line 21, so that the slide is always below the liquid nitrogen liquid level, the bottom of the groove of the upper bracket 111 is higher than the liquid nitrogen maximum horizontal line 22, the freezing storage bracket can be opened by hand, the freezing carrying rod is convenient to take out, the top of the upper bracket 111 is provided with a label 114, and the label 114 can be provided with different labels for marking and distinguishing different bracket group mechanisms.
A vertical freezing loading rod placing pore passage 115 is arranged on the base 14; the top height of the freezing slide rod placing hole 115 is not higher than the lowest liquid nitrogen level 21, and the freezing slide rod placing hole 115 is tightly attached to the upper bracket 111. The inner diameter of the freezing loading rod placing hole 115 is slightly larger than the diameter of the slide cap, and the slide cap is downwards inserted into the freezing loading rod placing hole 115, so that the freezing loading rod is placed.
The shelf 12 is generally the same length as the length of the refrigerated storage rack and generally the same height as the lower rack 112.
A slide cap clamp mechanism is provided on the base 14 for removing the slide cap of the freezing slide bar.
The slide cap clip mechanism includes a support post 131, an openable clip assembly 132, and a clamping bar 133 with a clamping handle 134, the support post 131, the clip assembly 132, and the clamping bar 133 all being made of a metal heat conductive material. The supporting column 131 is vertically fixed on the base 14, the clip assembly 132 is arranged at the top end of the supporting column 131, the clamping rod 133 is in a Z shape, two ends of the clamping rod 133 are respectively connected with the clamping handle 134 and the clip assembly 132, and the clamping handle 134 controls the opening/closing of the clip assembly 132 through the clamping rod 133. The height of the clamping piece assembly 132 does not exceed the lowest liquid nitrogen level 21, so that the clamping piece assembly 132 is always positioned below the liquid nitrogen level, and the clamping piece assembly 132 is in a cylindrical shell shape formed by two semi-cylindrical shell-shaped clamping pieces and can just clamp a cylindrical slide cap. The axis of the clamping piece component 132 and the horizontal plane form an included angle of 45 degrees, and the diameter of the clamping piece component 132 is slightly smaller than that of the slide cap, so that the slide cap is convenient to fix. The normal state of the clip assembly 132 is the open state.
The clamp handle 134 is located at the periphery of the heat preservation box body 2, and the clamp handle 134 is made of plastic and other non-heat conducting materials, so that an operator can conveniently hold and operate the clamp handle.
The method for simultaneously carrying out vitrification thawing on a plurality of patient gametes or embryos by using the device comprises the following steps:
1) placing the cleaned and sterilized bracket base device 1 into the inner cavity of the heat preservation box body 2, and aligning the fixing column 23 at the bottom of the inner cavity of the heat preservation box body 2 to the fixing hole 141 on the base 14;
2) pouring liquid nitrogen into the heat preservation box body 2, and enabling the liquid level of the liquid nitrogen to be located between the lowest horizontal line 21 and the highest horizontal line 22;
3) taking out the forceps 25 from the forceps groove 24, taking out the auxiliary reproductive cryopreservation device which needs to be thawed and is preserved in the liquid nitrogen tank, placing the cryopreservation bracket in the groove of the upper bracket 111 and the groove of the lower bracket 112, and enabling the end part of the cryopreservation bracket to be abutted against the supporting surface 113; the end of the freezing slide bar with the slide is close to the lower bracket 112, and the slide is positioned between the lower bracket 112 and the supporting surface 113; thus, the slides were in liquid nitrogen;
4) the label 114 with the serial number is arranged on the bracket group mechanism, so that different freezing storage brackets can be marked;
5) opening the freezing storage bracket, taking the freezing carrying rod together with the slide glass and the slide glass cap out of the freezing storage bracket by using forceps 25, inserting the slide glass cap downwards into the corresponding freezing carrying rod placing hole channel 115 for later use, wherein the slide glass is positioned in liquid nitrogen at the moment, and the tail end of the freezing carrying rod is positioned on the liquid level of the liquid nitrogen;
6) when the glass needs to be thawed, the tail end of the freezing carrying rod is held by the left hand, the glass carrying cap is placed into the clamping piece assembly 132 in the open state, the clamping rod 133 is driven by the right hand to close the clamping piece assembly 132 by adjusting the clamping handle 134, and the glass carrying cap is clamped by the clamping piece assembly 132 at the moment; in the process, the slide glass is positioned in liquid nitrogen;
then, the left hand pulls the freezing carrying rod outwards along the axial direction of the clamping piece assembly 132, so as to remove the carrying cap, and then quickly transfers the gametes or embryos on the carrying sheet to the conventional vitrification unfreezing liquid for unfreezing treatment.
Finally, it is also noted that the above-mentioned list is only a few specific embodiments of the present invention. Obviously, the present invention is not limited to the above embodiments, and many modifications are possible. All modifications which can be derived or suggested by a person skilled in the art from the disclosure of the invention should be considered as within the scope of the invention.
Claims (9)
1. Vitrification unfreezes liquid nitrogen box, the supporting supplementary reproduction that is used for containing freezing storage support freezes save set, its characterized in that: comprises a heat preservation box body (2) and a bracket base device (1) arranged in the inner cavity of the heat preservation box body (2); the top surface of the heat preservation box body (2) is provided with an opening;
the bracket base device (1) comprises a bracket group mechanism and a base (14) which are both made of metal heat conduction materials, and also comprises a slide glass cap clamp mechanism;
the base (14) is attached to the bottom surface of the inner cavity of the heat preservation box body (2);
the support group mechanism comprises an upper support (111), a lower support (112) and a supporting surface (113) which are all fixedly arranged on the base (14), the upper support (111), the lower support (112) and the supporting surface (113) are sequentially arranged, the central lines of the upper support (111), the lower support (112) and the supporting surface (113) are overlapped, and grooves are respectively arranged at the tops of the upper support (111) and the lower support (112); the distance between the groove of the upper bracket (111) and the supporting surface (113) is less than the length of the freezing storage bracket;
the slide cap clamp mechanism comprises a supporting column (131) and an openable clamping piece assembly (132) which are both made of metal heat conduction materials, and further comprises a clamping rod (133) with a clamping handle (134), the supporting column (131) is fixedly connected with the base (14), the clamping piece assembly (132) is arranged on the supporting column (131), and the clamping rod (133) is connected with the clamping piece assembly (132).
2. The vitrification thawing liquid nitrogen box according to claim 1, characterized in that:
the side wall of the inner cavity of the heat preservation box body (2) is respectively provided with a liquid nitrogen lowest horizontal line (21) and a liquid nitrogen highest horizontal line (22);
the bottom of the groove of the lower bracket (112) is not higher than the lowest level (21) of liquid nitrogen, and the bottom of the groove of the upper bracket (111) is higher than the highest level (22) of liquid nitrogen;
the height of the clip assembly (132) is no higher than the liquid nitrogen minimum level (21).
3. The vitrification thawing liquid nitrogen box according to claim 2, characterized in that:
a partition plate (12) is arranged in the middle of the base (14), and two sets of bracket set mechanisms are respectively arranged on two sides of the partition plate (12);
the center lines of the upper bracket (111), the lower bracket (112) and the supporting surface (113) are parallel to the clapboard (12).
4. The vitrification thawing liquid nitrogen box according to claim 2 or 3, characterized in that: the clamping rod (133) is Z-shaped, and the clamping handle (134) is positioned at the periphery of the heat preservation box body (2).
5. The vitrification thawing liquid nitrogen box according to claim 4, characterized in that:
a vertical freezing loading rod placing pore passage (115) is arranged on the base (14); the top height of the freezing loading rod placing hole (115) is not higher than the lowest liquid nitrogen level (21), and the freezing loading rod placing hole (115) is tightly attached to the upper bracket (111).
6. The vitrification thawing liquid nitrogen box according to claim 5, characterized in that:
the top of the upper bracket (111) is provided with a label (114).
7. The vitrification thawing liquid nitrogen box according to claim 6, characterized in that: the clamping piece assembly (132) is in a cylindrical shell shape consisting of two semi-cylindrical shell-shaped clamping pieces, and the axis of the clamping piece assembly (132) forms an included angle of 45 degrees with the base (14).
8. The vitrification thawing liquid nitrogen box according to claim 7, characterized in that: the outer side wall of the heat preservation box body (2) is provided with a forceps groove (24) for placing forceps (25).
9. The vitrification thawing liquid nitrogen box according to claim 8, characterized in that: a fixing column (23) is arranged on the bottom surface of the inner cavity of the heat preservation box body (2), and a through hole (141) matched with the fixing column (23) is arranged on the base (14).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021493792.8U CN213169058U (en) | 2020-07-23 | 2020-07-23 | Vitrification thawing liquid nitrogen box |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021493792.8U CN213169058U (en) | 2020-07-23 | 2020-07-23 | Vitrification thawing liquid nitrogen box |
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| Publication Number | Publication Date |
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| CN213169058U true CN213169058U (en) | 2021-05-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202021493792.8U Active CN213169058U (en) | 2020-07-23 | 2020-07-23 | Vitrification thawing liquid nitrogen box |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115009670A (en) * | 2022-06-27 | 2022-09-06 | 中国地质科学院水文地质环境地质研究所 | Sample transfer device for water quality monitoring and use method thereof |
-
2020
- 2020-07-23 CN CN202021493792.8U patent/CN213169058U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115009670A (en) * | 2022-06-27 | 2022-09-06 | 中国地质科学院水文地质环境地质研究所 | Sample transfer device for water quality monitoring and use method thereof |
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