CN216135089U - Freezing pole that carries - Google Patents
Freezing pole that carries Download PDFInfo
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- CN216135089U CN216135089U CN202121932433.2U CN202121932433U CN216135089U CN 216135089 U CN216135089 U CN 216135089U CN 202121932433 U CN202121932433 U CN 202121932433U CN 216135089 U CN216135089 U CN 216135089U
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- 238000007710 freezing Methods 0.000 title claims abstract description 126
- 230000008014 freezing Effects 0.000 title claims abstract description 126
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 206
- 239000007788 liquid Substances 0.000 claims description 138
- 238000010257 thawing Methods 0.000 claims description 16
- 230000007704 transition Effects 0.000 claims description 3
- 210000002257 embryonic structure Anatomy 0.000 abstract description 33
- 238000000034 method Methods 0.000 abstract description 22
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 14
- 239000002577 cryoprotective agent Substances 0.000 description 14
- 229910052757 nitrogen Inorganic materials 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 208000009701 Embryo Loss Diseases 0.000 description 1
- 206010042573 Superovulation Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 231100000557 embryo loss Toxicity 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011067 equilibration Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000009499 grossing Methods 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000004017 vitrification Methods 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a freezing carrying rod which comprises an embryo loading area, a freezing dropping area and an unfreezing embryo-moving area, wherein two sides of the embryo loading area are respectively communicated with the freezing dropping area and the unfreezing embryo-moving area, and the embryo loading area is positioned at the lowest position. In the process of using the freezing carrying rod for freezing operation, only one-time transfer operation is needed for embryos, and compared with the freezing carrying rod used in the existing freezing operation, the freezing carrying rod reduces the embryo transfer times and reduces the damage possibly caused to the embryos due to transfer. Moreover, because only one transfer operation is needed for the embryo, the automatic design of the operation is facilitated.
Description
Technical Field
The utility model relates to the technical field of embryo culture, in particular to a freezing carrying rod.
Background
In the course of assisted reproduction for infertility patients, the number of mature ova is generally increased by the stimulation of ovulation-promoting drugs, and the ova with a large number of ova can often grow at one time. But in practice only 1-3 embryos are transferred back into the human uterine cavity in one treatment session. Because implantation of too many embryos may increase the risk of multiple embryos, the remaining embryos may be cryopreserved. Embryo cryopreservation is a well-established method for preserving fertility. The technology is that equilibrium liquid and cryoprotectant are used for processing the embryo before freezing according to operation steps, so that the cryoprotectant gradually permeates into cells of the embryo, the embryo can bear the process of sudden temperature reduction to be happened without being damaged, and after a series of operation procedures, the embryo can reach a proper freezing state, namely, the embryo can be placed in a liquid nitrogen environment at the temperature of 196 ℃ below zero for long-time storage. If the treatment period is not successful, the preserved embryo can be transplanted back to the mother body after the later recovery, and the superovulation treatment process is not needed, so that the cost can be saved, and the chance of pregnancy of the patient can be increased.
At present, in the field of assisted reproduction, in the embryo culture process, the traditional embryo vitrification freezing method is used more generally, and the operation flow is generally as follows:
s1 preparing a balance solution and a culture dish;
s2 preparing balanced liquid drops in the culture dish;
s3 transfer the embryo into a balancing drop using a pasteur tube;
s4, standing the embryo in the equilibrium solution for 5 minutes;
s5 preparing cryoprotectant drops in a petri dish;
s6 transferring the embryos into cryoprotectant drops using pasteur tubes;
s7, standing the embryo in the cryoprotectant for 1 minute;
s8 transferring the embryo to the front end of the freezing carrying rod by using a Pasteur tube;
s9 the front end of the freezing carrying rod is quickly immersed into liquid nitrogen.
The operation flow of vitrifying and freezing the embryo has the following problems in the actual operation process:
because the embryos need to be transferred for many times by using the pasteur tubes in the operation process, the operation steps are complex, and the embryos can be damaged to a certain extent or lost, so that the treatment effect of embryo culture and assisted reproduction is influenced. In addition, two consumables, namely a culture dish and a freezing carrying rod, are needed to finish a freezing process.
SUMMERY OF THE UTILITY MODEL
The present invention is directed to a freezing bar that solves the problems set forth above in the background.
In order to achieve the purpose, the utility model provides the following technical scheme: the utility model provides a pole is carried in freezing, includes embryo loading district, freezing dropping liquid district and unfreezes and move embryo district, the embryo loading district both sides respectively with freezing dropping liquid district with unfreeze and move embryo district intercommunication, embryo loading district is in the lowest order. By designing the three containing areas, wherein the embryo loading area is designed at the lowest position and is used for placing embryos and positioning the embryos, the flowing of liquid is prevented in the freezing process, and the embryos are driven to move; the frozen drip area is used for the drip and removal of the balancing liquid and the cryoprotectant, and simultaneously ensures that less liquid remains in the embryo loading area during removal; the thawing embryo transfer zone is used to allow the embryos to be more easily shed when thawed, while ensuring that less liquid remains in the embryo loading zone during freezing.
In the above scheme, the embryo loading region is of a downward concave structure or an inverted conical structure.
In the scheme, one side of the embryo loading area, which is connected with the freezing dropping area, is a vertical surface. One side of the embryo loading area, which is connected with the freezing dropping area, is designed to be a vertical surface, so that the embryo can be effectively prevented from sliding to the freezing dropping area in the operation process, and the safety of the embryo is guaranteed; on the other hand, the volume of the embryo loading zone can be greatly reduced, thereby ensuring that as little liquid as possible remains during liquid removal and before freezing of the embryos.
In the scheme, one side of the embryo loading area, which is connected with the thawing embryo-transferring area, is an inclined plane or an arc surface. By designing the side of the embryo loading area connected with the embryo unfreezing and transferring area as a slope or a cambered surface, on one hand, embryos can be more easily shed during unfreezing, and on the other hand, liquid can be more easily flowed, so that less liquid is remained in the embryo loading area.
In the above protocol, the embryo loading zone has a volume of 0.1-0.9 ul.
In the above scheme, one side of the frozen dropping liquid area communicated with the embryo loading area is a plane structure. In use, the planar structure of the frozen droplet zone is arranged horizontally. Through designing the freezing dropping liquid area and embryo loading area one side that is linked together into planar structure, so made things convenient for the dropwise add and remove of liquid.
In the scheme, one side of the freezing dropping liquid area, which is far away from the embryo loading area, is of a cambered surface structure. Through the design of the cambered surface structure on the side of the freezing dropping liquid zone far away from the embryo loading zone, the operating space of the dropping liquid burette can be greatly increased, and the operation is facilitated.
In the above scheme, the joint of the plane structure and the arc-shaped structure of the freezing dropping liquid zone is arc-shaped. Because the head of dropping liquid buret is circular planar structure, so through the planar structure with freezing drip district with the junction design of ARC structure be arc, can better with the head contact of dropping liquid buret like this, simultaneously because the difficult residual liquid of convex corner, so be convenient for the removal of liquid, arc also is favorable to the production and processing moreover.
In the above scheme, the side of the cambered surface structure of the frozen dripping zone far away from the embryo loading zone inclines outwards. One side of keeping away from embryo loading district through the cambered surface structure with freezing drip district designs into and leans out, can further increase the operating space of dropping liquid buret like this to made things convenient for the operation, liquid spills over when also can preventing the operation simultaneously.
In the scheme, two sides of the embryo loading area are respectively connected with the freezing dropping liquid area and the unfreezing embryo transferring area in a smooth transition mode. The connection part of the embryo loading area, the frozen dripping area and the unfreezing embryo transferring area is smoothed, so that on one hand, the flowing of liquid is facilitated, and less liquid is remained in the embryo loading area; on the other hand, when the embryo is unfrozen, the embryo can be more easily fallen off and the embryo can not be scratched.
In the scheme, the unfreezing embryo transfer area is of a cambered surface structure. The unfreezing embryo-transferring area is designed to be of a cambered surface structure, so that the embryo can be further easily fallen off during unfreezing, and the quick separation of the embryo from the freezing carrying rod is facilitated.
In the scheme, the lowest height of the unfreezing embryo-moving area is greater than or equal to the height of the plane structure of the freezing dropping liquid area, and the height of the plane structure of the freezing dropping liquid area is greater than or equal to the highest height of the embryo loading area. By designing the lowest height of the unfreezing embryo-moving area to be greater than or equal to the height of the plane structure of the freezing dripping area and designing the height of the plane structure of the freezing dripping area to be greater than or equal to the highest height of the embryo loading area, the liquid removal is facilitated, and the residual liquid before the embryo is frozen is ensured to be as little as possible.
In the above scheme, the lateral dimension D1 of the embryo loading zone is smaller than the lateral dimension D2 of the frozen drip zone, and the lateral dimension D1 of the embryo loading zone is smaller than the lateral dimension D3 of the thawed embryo-moved zone. By minimizing the lateral dimension D1 of the embryo loading zone, the volume of the embryo loading zone can be further reduced, thereby further ensuring that as little liquid as possible remains during liquid removal and prior to freezing of the embryos.
In the above scenario, the lateral dimension D3 of the thawed embryo transfer zone is less than the lateral dimension D2 of the frozen droplet zone. By designing the transverse dimension D3 of the thawed embryo transfer zone to be less than the transverse dimension D2 of the frozen droplet zone, this facilitates rapid submersion of the frozen carrier rod into liquid nitrogen.
In the scheme, the outer wall of the freezing dropping liquid area is provided with an operating handle.
In the scheme, the volume of the freezing dropping liquid area is 8-14ul, and the volume of the unfreezing embryo transfer area is 8-14 ul.
The utility model provides a freezing loading rod. The method has the following beneficial effects:
1. by designing the three containing areas, wherein the embryo loading area is designed at the lowest position and is used for placing embryos and positioning the embryos, the flowing of liquid is prevented in the freezing process, and the embryos are driven to move; the frozen drip area is used for the drip and removal of the balancing liquid and the cryoprotectant, and simultaneously ensures that less liquid remains in the embryo loading area during removal; the unfreezing embryo-transferring area is used for enabling the embryo to fall off more easily during unfreezing, and meanwhile, less liquid is remained in the embryo loading area during freezing;
2. one side of the embryo loading area, which is connected with the freezing dropping area, is designed to be a vertical surface, so that the embryo can be effectively prevented from sliding to the freezing dropping area in the operation process, and the safety of the embryo is guaranteed; on the other hand, the volume of the embryo loading area can be greatly reduced, so that the residual liquid is ensured to be as little as possible when the liquid is removed and before the embryo is frozen;
3. the side, connected with the embryo unfreezing and transferring area, of the embryo loading area is designed to be an inclined plane or an arc surface, so that an embryo can fall off more easily during unfreezing, and liquid can flow more easily, and less liquid is left in the embryo loading area;
4. in use, the planar structure of the frozen droplet zone is arranged horizontally. One side of the freezing dropping liquid area communicated with the embryo loading area is designed to be a plane structure, so that dropping and removing of liquid are facilitated;
5. one side of the freezing dropping liquid zone, which is far away from the embryo loading zone, is designed into a cambered surface structure, so that the operation space of the dropping liquid burette can be greatly increased, and the operation is convenient;
6. the head of the dropping liquid burette is of a circular structure, so that the joint of the plane structure of the freezing dropping liquid area and the cambered surface structure is designed to be in a circular arc shape, the head of the dropping liquid burette can be better matched with the head of the freezing dropping liquid burette, and meanwhile, liquid is not easy to remain at the corners of the circular arc shape, so that the liquid is convenient to remove, and the circular arc shape is also beneficial to production and processing;
7. one side of the cambered surface structure of the freezing dropping liquid zone, which is far away from the embryo loading zone, is designed to be inclined outwards, so that the operating space of the dropping liquid burette can be further increased, the operation is convenient, and meanwhile, the liquid can be prevented from overflowing during the operation;
8. the connection part of the embryo loading area, the frozen dripping area and the unfreezing embryo transferring area is smoothed, so that on one hand, the flowing of liquid is facilitated, and less liquid is remained in the embryo loading area; on the other hand, when unfreezing, the embryo can be more easily fallen off and can not be scratched;
9. the unfreezing embryo-transferring area is designed into a cambered surface structure, so that the embryos can be further easily dropped during unfreezing, and the embryos can be favorably and rapidly separated from the freezing carrying rod;
10. the lowest height of the unfreezing embryo-moving area is designed to be larger than or equal to the height of the plane structure of the freezing dripping area, and the height of the plane structure of the freezing dripping area is designed to be larger than or equal to the highest height of the embryo loading area, so that the liquid is convenient to remove, and the residual liquid before the embryo is frozen is ensured to be as little as possible;
11. the transverse dimension D1 of the embryo loading zone is designed to be minimum, so that the volume of the embryo loading zone can be further reduced, and the residual liquid during liquid removal and before embryo freezing is further ensured to be as little as possible;
12. the transverse dimension D3 of the unfreezing embryo transfer area is designed to be smaller than the transverse dimension D2 of the freezing dripping area, so that the freezing carrying rod is conveniently and rapidly immersed into liquid nitrogen;
13. in the process of using the freezing carrying rod for freezing operation, the embryo is only required to be transferred once, compared with the freezing carrying rod used in the existing freezing operation, the embryo transfer frequency is reduced, the damage to the embryo caused by transfer is reduced, and in addition, the embryo is only required to be transferred once, so that the automatic design of operation is facilitated.
Drawings
FIG. 1 is a schematic view of the present freezing bar;
FIG. 2 is a schematic cross-sectional view of FIG. 1;
FIG. 3 is a partially enlarged schematic view of FIG. 2;
fig. 4 is a partially enlarged schematic view of fig. 1.
In the figure: an embryo loading area 1, a frozen dripping area 2, a thawing embryo transferring area 3 and an operating handle 4.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
As shown in fig. 1 to 4, the present invention provides a technical solution: a freezing and carrying rod comprises an embryo loading area 1, a freezing dropping liquid area 2 and an unfreezing and embryo-transferring area 3, wherein two sides of the embryo loading area 1 are respectively communicated with the freezing dropping liquid area 2 and the unfreezing and embryo-transferring area 3, and the embryo loading area 1 is located at the lowest position. By designing three containing areas, wherein the embryo loading area 1 is designed at the lowest position and is used for placing embryos and positioning embryos, the flowing of liquid is prevented in the freezing process, and the embryos are driven to move; the frozen drip area 2 is used to balance the dripping and removal of liquid and cryoprotectant while ensuring that less liquid remains in the embryo loading area 1 upon removal; the thawing embryo transfer section 3 is used to allow the embryos to be more easily shed when thawing, while ensuring that less liquid remains in the embryo loading section 1 during freezing.
The embryo loading region 1 is of a downward concave structure or an inverted cone structure. One side of the embryo loading area 1 connected with the freezing dropping liquid area 2 is a vertical surface. One side of the embryo loading area 1 connected with the freezing dripping area 2 is designed to be a vertical surface, so that the embryo can be effectively prevented from sliding to the freezing dripping area 2 in the operation process, and the safety of the embryo is guaranteed; on the other hand, the volume of the embryo loading zone 1 can be greatly reduced, thereby ensuring that as little liquid as possible remains during liquid removal and before freezing of the embryos. The side of the embryo loading area 1 connected with the thawing embryo-transferring area 3 is an inclined plane or an arc surface. By designing the side of the embryo loading region 1 connected with the embryo unfreezing and transferring region 3 as a slope or a cambered surface, the embryo can be more easily fallen off during unfreezing on the one hand, and the liquid can be more easily flowed on the other hand, so that less liquid remains in the embryo loading region 1. The volume of the embryo loading area 1 is 0.1-0.9 ul.
One side of the frozen dripping area 2 communicated with the embryo loading area 1 is of a plane structure. In use, the planar configuration of the frozen droplet zone 2 is arranged horizontally. By designing the side of the freezing dropping liquid area 2 communicated with the embryo loading area 1 to be a plane structure, the dropping and the removal of liquid are convenient. The side of the freezing dropping liquid area 2 far away from the embryo loading area 1 is of a cambered surface structure. One side of the freezing dropping liquid zone 2, which is far away from the embryo loading zone 1, is designed into a cambered surface structure, so that the operation space of the dropping liquid burette can be greatly increased, and the operation is convenient. The joint of the plane structure and the cambered surface structure of the freezing dripping area 2 is arc-shaped. Because the head of dropping liquid buret is circular structure, so through the junction design for arc with the planar structure of freezing dropping liquid district 2 and ARC structure, can better like this with the head adaptation of dropping liquid buret, simultaneously because the difficult residual liquid of convex corner, so be convenient for the removal of liquid, arc also is favorable to the production and processing moreover. The cambered surface structure of the freezing dropping liquid zone 2 inclines outwards from one side of the embryo loading zone 1. One side of keeping away from embryo loading district 1 through the cambered surface structure with freezing drip district 2 designs into and leans out, can further increase the operating space of dropping liquid buret like this to make things convenient for the operation, liquid spills over when also can preventing the operation simultaneously.
The two sides of the embryo loading area 1 are respectively connected with the freezing dropping liquid area 2 and the unfreezing embryo transferring area 3 in a smooth transition way. The connection part of the embryo loading area 1, the frozen dripping area 2 and the unfreezing embryo-transferring area 3 is smoothed, so that on one hand, the flowing of liquid is facilitated, and less liquid is left in the embryo loading area 1; on the other hand, when the embryo is unfrozen, the embryo can be more easily fallen off and the embryo can not be scratched. The unfreezing embryo transfer area 3 is of a cambered surface structure. The unfreezing embryo-moving area 3 is designed to be of a cambered surface structure, so that the embryo can be further easily fallen off during unfreezing, and the quick separation of the embryo from the freezing carrying rod is facilitated.
The lowest height of the unfreezing embryo-transferring area 3 is larger than or equal to the height of the plane structure of the freezing dropping liquid area 2, and the height of the plane structure of the freezing dropping liquid area 2 is larger than or equal to the highest height of the embryo loading area 1. By designing the lowest height of the thawing embryo transfer zone 3 to be greater than or equal to the height of the planar structure of the freezing drip zone 2 and the height of the planar structure of the freezing drip zone 2 to be greater than or equal to the highest height of the embryo loading zone 1, the removal of liquid is facilitated, thereby ensuring that the residual liquid before the embryo is frozen is as little as possible.
The transverse dimension D1 of the embryo loading zone 1 is smaller than the transverse dimension D2 of the frozen dripping zone 2, and the transverse dimension D1 of the embryo loading zone 1 is smaller than the transverse dimension D3 of the unfrozen embryo transfer zone 3. The minimal design of the lateral dimension D1 of the embryo loading zone 1 further reduces the volume of the embryo loading zone 1, thereby further ensuring that as little liquid as possible remains during liquid removal and prior to freezing of the embryos. The lateral dimension D3 of the thawed embryo transfer zone 3 is less than the lateral dimension D2 of the frozen droplet zone 2. By designing the transverse dimension D3 of the thawing embryo transfer region 3 to be smaller than the transverse dimension D2 of the freezing droplet region 2, rapid immersion of the freezing carrying rod into liquid nitrogen is facilitated.
An operating handle 4 is arranged on the outer wall of the freezing dropping liquid area 2. The volume of the freezing dropping liquid area 2 is 8-14ul, and the volume of the unfreezing embryo transferring area 3 is 8-14 ul.
In the embodiment, three containing areas are designed, wherein the embryo loading area 1 is designed at the lowest position and is used for placing embryos and positioning embryos, so that the flowing of liquid is prevented in the freezing process, and the embryos are driven to move; the frozen drip area 2 is used to balance the dripping and removal of liquid and cryoprotectant while ensuring that less liquid remains in the embryo loading area 1 upon removal; the thawing embryo transfer section 3 is used to allow the embryos to be more easily shed when thawing, while ensuring that less liquid remains in the embryo loading section 1 during freezing.
Meanwhile, in the embodiment, one side of the embryo loading area 1 connected with the freezing dripping area 2 is designed to be a vertical surface, so that the embryo can be effectively prevented from sliding to the freezing dripping area 2 in the operation process, and the safety of the embryo is guaranteed; on the other hand, the volume of the embryo loading area 1 can be greatly reduced, so that the residual liquid is ensured to be as little as possible when the liquid is removed and before the embryo is frozen; the side of the embryo loading area 1 connected with the unfreezing and embryo transferring area 3 is designed to be an inclined plane or an arc surface, so that an embryo can fall off more easily during unfreezing, and liquid can flow more easily, and less liquid remains in the embryo loading area 1;
moreover, in the embodiment, one side of the frozen dripping area 2 communicated with the embryo loading area 1 is designed to be a plane structure, so that the dripping and the removal of liquid are convenient; one side of the freezing dropping liquid zone 2, which is far away from the embryo loading zone 1, is designed into a cambered surface structure, so that the operation space of the dropping liquid burette can be greatly increased, and the operation is convenient; the head of the dropping liquid burette is of a circular structure, so that the joint of the plane structure of the freezing dropping liquid zone 2 and the cambered surface structure is designed to be in a circular arc shape, the head of the dropping liquid burette can be better matched with the joint of the plane structure and the cambered surface structure, and meanwhile, liquid is not easy to remain at the corners of the circular arc shape, so that the liquid is convenient to remove, and the circular arc shape is also beneficial to production and processing; one side of keeping away from embryo loading district 1 through the cambered surface structure with freezing drip district 2 designs into and leans out, can further increase the operating space of dropping liquid buret like this to make things convenient for the operation, liquid spills over when also can preventing the operation simultaneously.
In addition, in the embodiment, the connection part of the embryo loading area 1, the frozen dripping area 2 and the unfreezing embryo transferring area 3 is subjected to smoothing treatment, so that on one hand, the flowing of liquid is facilitated, and less liquid is remained in the embryo loading area 1; on the other hand, when unfreezing, the embryo can be more easily fallen off and can not be scratched; the unfreezing embryo-moving area 3 is designed to be of a cambered surface structure, so that the embryo can be further easily fallen off during unfreezing, and the quick separation of the embryo from the freezing carrying rod is facilitated.
Finally, the present embodiment is designed to have the lowest height of the thawing embryo transfer area 3 greater than or equal to the height of the planar structure of the freezing drip area 2, and the height of the planar structure of the freezing drip area 2 greater than or equal to the highest height of the embryo loading area 1, so as to facilitate the removal of liquid, thereby ensuring that the residual liquid before the embryo is frozen is as little as possible; the transverse dimension D1 of the embryo loading zone 1 is designed to be minimum, so that the volume of the embryo loading zone 1 can be further reduced, and the residual liquid during liquid removal and before embryo freezing is further ensured to be as little as possible; by designing the transverse dimension D3 of the thawing embryo transfer region 3 to be smaller than the transverse dimension D2 of the freezing droplet region 2, rapid immersion of the freezing carrying rod into liquid nitrogen is facilitated.
Example 2
As shown in fig. 1 to 4, the present invention provides a technical solution: a method for operating a freezing bar comprises the following steps,
s1 transferring the embryo together with the culture solution into the freezing drip area 2 of the freezing carrying rod;
s2 removing excess culture fluid using pasteur tubes;
s3, sucking 20ul of balancing liquid by using a Pasteur tube, and dripping the balancing liquid into the freezing liquid dripping area 2 to ensure that the balancing liquid can completely submerge the embryo;
s4, standing the embryo in the equilibrium solution for 5 minutes;
s5 removing the equilibration fluid using a pasteur tube;
s6 repeating S3-S5 for several times if necessary;
s7, sucking 20ul of cryoprotectant by using a Pasteur tube, and dripping the cryoprotectant into the freezing drip area 2 to ensure that the cryoprotectant can completely submerge the embryo;
s8 standing the embryo in cryoprotectant for 1 min;
s9 removing cryoprotectant using a pasteur tube;
s10 repeating S6-S8 for several times if necessary;
s11 rapidly submerges the freezing bar into liquid nitrogen.
The operation steps show that in the process of freezing operation by using the freezing carrying rod, only one transfer operation is needed to be carried out on embryos, and compared with the freezing carrying rod used in the existing freezing operation, the embryo transfer frequency is reduced, and possible damage and embryo loss caused by transfer to the embryos are reduced. Moreover, because only one transfer operation is needed for the embryo, the automatic design of the operation is facilitated.
Claims (10)
1. A frozen bar, comprising: the embryo thawing and transferring device comprises an embryo loading area (1), a freezing dripping area (2) and an thawing and transferring embryo area (3), wherein two sides of the embryo loading area (1) are respectively communicated with the freezing dripping area (2) and the thawing and transferring embryo area (3), and the embryo loading area (1) is located at the lowest position.
2. A freeze carrier bar as claimed in claim 1, wherein: the embryo loading area (1) is of a downward concave structure or an inverted cone structure.
3. A freeze carrier bar as claimed in claim 2, wherein: the side, connected with the freezing dripping zone (2), of the embryo loading zone (1) is a vertical surface, and the side, connected with the thawing embryo transferring zone (3), of the embryo loading zone (1) is an inclined surface or an arc surface.
4. A frozen carrier bar as claimed in claim 3, wherein: one side of the freezing dropping liquid area (2) communicated with the embryo loading area (1) is of a plane structure, one side of the freezing dropping liquid area (2) far away from the embryo loading area (1) is of an arc-shaped surface structure, and the joint of the plane structure of the freezing dropping liquid area (2) and the arc-shaped surface structure is of an arc shape.
5. A freeze carrier bar as claimed in claim 4, wherein: the cambered surface structure of the freezing dripping zone (2) is inclined outwards at one side far away from the embryo loading zone (1).
6. A freeze carrier bar as claimed in claim 5, wherein: the two sides of the embryo loading area (1) are respectively connected with the freezing dropping liquid area (2) and the unfreezing embryo transferring area (3) in a smooth transition mode.
7. A freeze carrier bar as claimed in claim 6, wherein: the unfreezing embryo transfer area (3) is of a cambered surface structure.
8. A frozen carrier bar as claimed in claim 7, wherein: the lowest height of the unfreezing embryo-moving area (3) is greater than or equal to the height of the plane structure of the freezing dripping area (2), and the height of the plane structure of the freezing dripping area (2) is greater than or equal to the highest height of the embryo loading area (1).
9. A frozen carrier bar as claimed in claim 8, wherein: the lateral dimension D1 of the embryo loading area (1) is smaller than the lateral dimension D2 of the frozen dripping area (2), the lateral dimension D1 of the embryo loading area (1) is smaller than the lateral dimension D3 of the unfrozen embryo transfer area (3), and the lateral dimension D3 of the unfrozen embryo transfer area (3) is smaller than the lateral dimension D2 of the frozen dripping area (2).
10. A freeze carrier bar as claimed in claim 1, wherein: the volume of the embryo loading area (1) is 0.1-0.9ul, the volume of the freezing dropping liquid area (2) is 8-14ul, and the volume of the unfreezing embryo transferring area (3) is 8-14 ul.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121932433.2U CN216135089U (en) | 2021-08-17 | 2021-08-17 | Freezing pole that carries |
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|---|---|---|---|
| CN202121932433.2U CN216135089U (en) | 2021-08-17 | 2021-08-17 | Freezing pole that carries |
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| CN216135089U true CN216135089U (en) | 2022-03-29 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113632785A (en) * | 2021-08-17 | 2021-11-12 | 武汉互创联合科技有限公司 | Freezing carrying rod and operation method thereof |
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2021
- 2021-08-17 CN CN202121932433.2U patent/CN216135089U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113632785A (en) * | 2021-08-17 | 2021-11-12 | 武汉互创联合科技有限公司 | Freezing carrying rod and operation method thereof |
| CN113632785B (en) * | 2021-08-17 | 2024-04-05 | 武汉互创联合科技有限公司 | Freezing carrying rod and operation method thereof |
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