CN217578842U - Diffusion box for microbial cultivation - Google Patents
Diffusion box for microbial cultivation Download PDFInfo
- Publication number
- CN217578842U CN217578842U CN202220450260.9U CN202220450260U CN217578842U CN 217578842 U CN217578842 U CN 217578842U CN 202220450260 U CN202220450260 U CN 202220450260U CN 217578842 U CN217578842 U CN 217578842U
- Authority
- CN
- China
- Prior art keywords
- hollow cylindrical
- porous support
- support main
- filter membrane
- frame
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000009792 diffusion process Methods 0.000 title claims abstract description 15
- 230000000813 microbial effect Effects 0.000 title claims description 7
- 239000012528 membrane Substances 0.000 claims abstract description 23
- 244000005700 microbiome Species 0.000 claims abstract description 22
- 239000004677 Nylon Substances 0.000 claims abstract description 13
- 229920001778 nylon Polymers 0.000 claims abstract description 13
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000000741 silica gel Substances 0.000 claims abstract description 7
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 7
- 238000007789 sealing Methods 0.000 claims abstract description 4
- 239000001963 growth medium Substances 0.000 claims description 6
- 239000004033 plastic Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims 3
- 239000000463 material Substances 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 239000002699 waste material Substances 0.000 abstract description 3
- 231100000252 nontoxic Toxicity 0.000 abstract description 2
- 230000003000 nontoxic effect Effects 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 238000011065 in-situ storage Methods 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000007797 corrosion Effects 0.000 description 2
- 238000005260 corrosion Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of microorganism experimental tool manufacturing, in particular to a diffusion box for microorganism culture, which has reasonable structure, low cost and simple and convenient assembly, and is characterized in that a porous support main body is arranged, the porous support main body comprises a frame type support frame and a plurality of hollow cylindrical holding cavities arranged in the frame type support frame, the upper end and the lower end of each hollow cylindrical holding cavity are open, and more than two hollow cylindrical holding cavities are fixed in the frame type support frame at equal intervals; porous support main part bottom is equipped with the aseptic nylon filter membrane that is used for sealing cavity tube-shape holding chamber, and aseptic nylon filter membrane bonds fixedly through organic silica gel and porous support main part bottom surface, compares with prior art, and porous support main part has can be high temperature resistant, and is waterproof, safe nontoxic characteristic and advantage, can used repeatedly, has both saved consumptive material resource, has avoided the waste, can avoid the pollution simultaneously again.
Description
Technical Field
The utility model relates to a microbial experiment apparatus makes technical field, specific microorganism that says so is cultivateed with diffusion box rational in infrastructure, with low costs, assemble portably.
Background
At present, the separation culture of marine microorganisms is mainly based on traditional dilution coating separation, however, most of microorganisms in the natural environment still cannot realize separation culture under laboratory conditions. In recent years, the development of emerging microbial culture omics technologies such as in-situ culture, enrichment culture and microfluidic technology greatly promotes the isolated culture of prokaryotic microorganisms. Among them, the isolation chip (ichip) is a better device for simulating in-situ environment, and can better separate the microorganisms which are difficult to culture or not cultured. Research personnel can utilize the separation chip device to develop in-situ culture to simulate the natural environment of microorganism growth, obtain microorganism groups which are difficult to grow on an artificial culture medium through separation culture, and find important secondary metabolites.
The existing diffusion box chip device is generally made of metal materials in a professional customization mode, on one hand, most researchers or laboratories do not have production conditions, on the other hand, the diffusion box chip made of metal has the defects that the diffusion box chip is easy to rust, and the metal can corrode along with the lapse of time when being soaked in water, particularly in saline water environments such as seawater or salt pan for a long time.
Disclosure of Invention
The utility model provides a rational in infrastructure, with low costs, assemble simple and convenient microbial cultivation with diffusion box to the shortcoming and not enough that exist among the prior art.
The utility model discloses a following measure reaches:
a diffusion box for microbial cultivation is characterized by being provided with a porous support main body, wherein the porous support main body comprises a frame type support frame and a plurality of hollow cylindrical accommodating cavities arranged in the frame type support frame, the upper ends and the lower ends of the hollow cylindrical accommodating cavities are open, and more than two hollow cylindrical accommodating cavities are fixed in the frame type support frame at equal intervals; the bottom of the porous support main body is provided with a sterile nylon filter membrane used for sealing the hollow cylindrical accommodating cavity, and the sterile nylon filter membrane is bonded and fixed with the bottom surface of the porous support main body through organic silica gel.
The interior footpath scope of cavity tube-shape holding chamber is 4mm to 5mm, and the interval scope in two adjacent cavity tube-shape holding chambers is 1mm to 2mm, is equipped with the floor that is central symmetry setting around cavity tube-shape holding chamber axis on every cavity tube-shape holding chamber outer wall, and adjacent cavity tube-shape holding chamber links to each other through the floor.
The utility model is also provided with an upper cover plate which adopts a sterile organic filter membrane with the aperture of 0.22 mu m.
The utility model discloses the porous support main part adopts plastics to make, and the internal preferred 96 well cavity tube-shape holding chambeies that are equipped with equidistant range of porous support main part form 96 independent cavities after the port under the aseptic nylon filter membrane of bottom seals, are used for holding the culture medium and wait to cultivate the microorganism sample during the use.
Compared with the prior art, the utility model, the porous bracket main body has the characteristics and advantages of high temperature resistance, water resistance, safety and innocuity, can avoid high temperature melting and damage when using high temperature high pressure sterilization, and the size of 96 holes is uniform, the position and the arrangement can just correspond to 8 rows of pipettors, and the sample adding efficiency can be greatly improved; the upper side and the lower side of the porous support main body are covered by sterile organic filter membranes (0.22 mu m), and are bonded and sealed by common organic glass silica gel on the market, so that the whole device is simple in manufacturing and material taking, low in cost, extremely available and capable of being repeatedly used, material resources are saved, waste is avoided, and pollution can be avoided; the device has appropriate size and volume and is very suitable for the growth and culture of microorganisms. In addition, the used sterile organic filter membrane is beneficial to the exchange of substances and molecules between microorganisms in the pores and the outside, and can prevent the outside microorganisms from entering; has the advantages of water resistance, good toughness, corrosion resistance and the like.
Description of the drawings:
fig. 1 is a schematic structural diagram of the present invention.
Reference numerals: frame support frame 1, cavity tube-shape holding chamber 2, aseptic organic filter membrane 3, floor 4, upper cover plate (aseptic organic filter membrane) 5.
The specific implementation mode is as follows:
the present invention will be further described with reference to the accompanying drawings and examples.
As shown in the attached figure 1, the utility model provides a diffusion box for microorganism culture, wherein, a porous support main body is provided, the porous support main body comprises a frame type support frame 1 and a plurality of hollow cylindrical holding cavities 2 arranged in the frame type support frame 1, the upper ends and the lower ends of the hollow cylindrical holding cavities 2 are open, and more than two hollow cylindrical holding cavities 2 are fixed in the frame type support frame 1 at equal intervals; the bottom of the porous support main body is provided with a sterile nylon filter membrane 3 for sealing the hollow cylindrical accommodating cavity, and the sterile nylon filter membrane 3 is fixedly bonded with the bottom surface of the porous support main body through organic silica gel; rib plates 4 arranged around the central axis of the hollow cylindrical accommodating cavity in a central symmetry manner are arranged on the outer wall of the hollow cylindrical accommodating cavity 2, and the adjacent hollow cylindrical accommodating cavities 2 are connected through the rib plates 4; an upper cover plate 5 is also arranged, and the upper cover plate 5 adopts a sterile organic filter membrane with the pore size of 0.22 mu m;
example 1:
the embodiment provides a diffusion box for microorganism culture, which is provided with a porous support main body, wherein the porous support main body comprises a frame type support frame 1 and a plurality of hollow cylindrical accommodating cavities 2 arranged in the frame type support frame 1, the upper ends and the lower ends of the hollow cylindrical accommodating cavities 2 are open, and more than two hollow cylindrical accommodating cavities 2 are fixed in the frame type support frame 1 at equal intervals; the bottom of the porous support main body is provided with a sterile nylon filter membrane 3 for closing the hollow cylindrical accommodating cavity, and the sterile nylon filter membrane 3 is bonded and fixed with the bottom surface of the porous support main body through organic silica gel; ribbed plates 4 which are arranged around the central axis of the hollow cylindrical accommodating cavity in a central symmetry manner are arranged on the outer wall of the hollow cylindrical accommodating cavity 2, and the adjacent hollow cylindrical accommodating cavities 2 are connected through the ribbed plates 4; the culture medium and the culture medium are characterized by further comprising an upper cover plate 5, the upper cover plate 5 is a sterile organic filter membrane with the thickness of 0.22 mu m, the porous support body is made of plastics, 96 hollow cylindrical accommodating cavities which are arranged at equal intervals are arranged in the porous support body, and 96 independent cavities are formed after the lower end of the porous support body is sealed by the bottom sterile nylon filter membrane and used for accommodating a culture medium and a microbial sample to be cultured.
Compared with the prior art, the utility model, the porous bracket main body can resist high temperature, is waterproof, has safe and nontoxic characteristics and advantages, can avoid high temperature melting and damage when using high temperature high pressure sterilization, has uniform size of 96 holes, can just correspond to 8 continuous pipettors in position and arrangement, and can greatly improve the sampling efficiency; the upper side and the lower side of the porous support main body are covered by sterile organic filter membranes (0.22 mu m), and are bonded and sealed by common organic glass silica gel on the market, so that the whole device is simple in manufacturing and material taking, low in cost, extremely available and capable of being repeatedly used, material resources are saved, waste is avoided, and pollution can be avoided; the device has appropriate size and volume, and is very suitable for the growth and culture of microorganisms. In addition, the sterile organic filter membrane is beneficial to the exchange of substances and molecules between microorganisms in the pores and the outside, and can prevent the outside microorganisms from entering; has the advantages of water resistance, good toughness, corrosion resistance and the like.
Claims (4)
1. A diffusion box for microbial cultivation is characterized by being provided with a porous support main body, wherein the porous support main body comprises a frame type support frame and a plurality of hollow cylindrical accommodating cavities arranged in the frame type support frame, the upper ends and the lower ends of the hollow cylindrical accommodating cavities are open, and more than two hollow cylindrical accommodating cavities are fixed in the frame type support frame at equal intervals; porous support main part bottom is equipped with the aseptic nylon filter membrane that is used for sealing cavity tube-shape holding chamber, and aseptic nylon filter membrane is fixed with porous support main part bottom surface bonding via quick-witted silica gel.
2. The diffusion box for culturing microorganisms according to claim 1, wherein the inner diameter of the hollow cylindrical receiving cavity ranges from 4mm to 5mm, the distance between two adjacent hollow cylindrical receiving cavities ranges from 1mm to 2mm, the outer wall of each hollow cylindrical receiving cavity is provided with ribs arranged in central symmetry around the central axis of the hollow cylindrical receiving cavity, and the adjacent hollow cylindrical receiving cavities are connected through the ribs.
3. The diffusion cassette for culturing microorganisms according to claim 1, further comprising an upper cover plate, wherein the upper cover plate is a sterile organic filter membrane having a thickness of 0.22 μm.
4. The diffusion box for culturing microorganisms according to claim 1, wherein the porous frame body is made of plastic, 96 hollow cylindrical holding cavities are arranged in the porous frame body at equal intervals, and 96 independent cavities are formed after the lower port of the porous frame body is sealed by a bottom sterile nylon filter membrane, and are used for holding culture media and microorganism samples to be cultured.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220450260.9U CN217578842U (en) | 2022-03-03 | 2022-03-03 | Diffusion box for microbial cultivation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220450260.9U CN217578842U (en) | 2022-03-03 | 2022-03-03 | Diffusion box for microbial cultivation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217578842U true CN217578842U (en) | 2022-10-14 |
Family
ID=83538342
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220450260.9U Expired - Fee Related CN217578842U (en) | 2022-03-03 | 2022-03-03 | Diffusion box for microbial cultivation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217578842U (en) |
-
2022
- 2022-03-03 CN CN202220450260.9U patent/CN217578842U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20221014 |
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| CF01 | Termination of patent right due to non-payment of annual fee |