CN213624077U - Mineral-mediated algae and/or microorganism allelopathy co-culture device - Google Patents
Mineral-mediated algae and/or microorganism allelopathy co-culture device Download PDFInfo
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- CN213624077U CN213624077U CN202021982643.8U CN202021982643U CN213624077U CN 213624077 U CN213624077 U CN 213624077U CN 202021982643 U CN202021982643 U CN 202021982643U CN 213624077 U CN213624077 U CN 213624077U
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Abstract
The utility model provides a mineral mediates the intercropping effect coculture device of algae and/or microorganism, and concretely field relates to mineral material and the interact between algae or the microorganism, mainly comprises transparent culture box, be equipped with two sets of detachable partition plate devices in the middle of the transparent culture box, every set of detachable partition plate device comprises two baffles and the filter membrane of clamp between two baffles. The device can realize the relatively independent culture of different algae or microorganisms, so as to research the allelopathy among the algae or the microorganisms, realize the research on the allelopathy mediated by the environmental nano mineral material and the influence on the conversion of the mineral material when the algae or the microorganisms interact with each other, monitor electrochemical signals in real time by connecting the intermediate chamber to an electrochemical workstation, realize the in-situ three-dimensional fluorescence analysis of the intermediate chamber and the analysis of metabolites, and simultaneously determine the transformation of mineral phases or forms in real time.
Description
Technical Field
The utility model relates to an alga or microorganism culture technical field, in particular to a mineral mediates the intercropping effect coculture device of alga and/or microorganism.
Background
Allelopathy between algae or between microorganisms is a key step in the study of microbial interactions, and specifically includes the effect of signal molecules released between algae on each other's growth metabolism. With the aggravation of the environmental pollution condition of water bodies, most previous researches only focus on the toxic influence of pollutants on single aquatic species to evaluate ecological risks, the transformation of minerals in the environment caused by allelopathy between algae or microorganisms or the mediation of the allelopathy between the microorganisms of the minerals is not clear at present, and therefore, the research on the allelopathy between the algae or the algae and other microorganisms under the external stress and the role of the minerals in the environment are particularly important. The existing methods for co-culturing microorganisms include culturing in a conical flask by using a dialysis bag, separating the two species from each other by direct contact, or separating the two species by using a single diaphragm, and such methods either do not facilitate the storage and taking of algae, are difficult to maintain the uniformity of experimental conditions of internal and external microorganisms, and further fail to detect the growth state of algae in real time, do not facilitate the research on the mediation effect or the transformation effect of allelochemicals on minerals, and do not facilitate the research on the interaction of non-direct contact of minerals and organisms.
SUMMERY OF THE UTILITY MODEL
In view of the above, the present invention provides a mineral-mediated algae and/or microorganism allelopathy co-culture apparatus, which can realize controllable and uniform experimental conditions and facilitate the storage and retrieval of algae.
The utility model provides a following technical scheme: a mineral-mediated algae and/or microorganism allelopathy co-culture device mainly comprises a transparent culture box, wherein two sets of detachable partition plate devices are arranged in the middle of the transparent culture box, and each set of detachable partition plate device comprises two partition plates and a filter membrane clamped between the two partition plates.
The removable baffle device divides the transparent culture box into three compartments.
The detachable clapboard devices are respectively inserted into the grooves at the bottom and the side of the transparent culture box for fixing.
And the partition plate is provided with filter membrane holes, and the filter membrane holes are matched with filter membranes with preset apertures and sizes.
The filter membrane hole is equipped with the screw hole all around, the screw hole is used for fixed baffle and filter membrane.
A cover plate is arranged above the transparent culture box.
The cover plate is provided with a vent hole.
The utility model has the advantages that:
the utility model discloses a physical contact between both sides alga or the microorganism is kept apart to the baffle, makes the allelochemical of release pass through the membrane to this allelochemical between the research alga is acted, and can be in order to satisfy different experimental demands through the aperture that changes the membrane. In addition, the existence of the middle compartment can detect the product of the culture solution of the middle compartment in real time, materials such as minerals can be added to study the role of the materials in the allelopathy of microorganisms and the mineral conversion process caused by the action, and devices such as electrochemistry, three-dimensional fluorescence and the like can be introduced to detect the middle compartment in real time. The whole body of the experimental device is transparent, the photosynthesis of the algae is not influenced, and the growth conditions of the algae on the two sides of the partition board are completely consistent.
Drawings
The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention without limitation. In the drawings:
FIG. 1: the structure of the utility model is schematically shown.
The labels in the figures are: 1. a vent hole; 2. a cover plate; 3. a compartment A; 4. a compartment B; 5. a middle compartment; 6. a filter membrane hole; 7. and (4) screw holes.
Detailed Description
The technical solution in the embodiments of the present invention will be removed and completely described below with reference to the schematic structural diagram of the present invention, and the described embodiments are only some embodiments, not all embodiments of the present invention.
As shown in figure 1, the utility model discloses a set of microorganism is culture apparatus altogether, including a hollow culture box type box, be equipped with two sets of detachable partition plate devices in the middle of the culture box, every set of detachable partition plate device comprises two baffles and the filter membrane of clamp between two baffles, detachable partition plate device will transparent culture box separates for three compartment, compartment A3, compartment B4, middle compartment 5, two baffle below have a circular opening, and circular opening is equipped with screw hole 7 all around.
Wherein, the fixed clapboard can improve the stability of the support of the culture device and the independence of the two culture chambers; the detachable partition plate can improve the flexibility of replacing the membrane; the screw holes are fixed by screws, so that the detachable partition plate, the membrane and the fixed partition plate can be tightly combined.
The intermediate compartment 6 mainly functions as: when no substance is added, a certain amount of liquid can be taken out in real time for product analysis; when mineral materials are added, the mediation of minerals in the action of microbial allelopathy can be researched; when mineral materials are added, a certain amount of suspension can be taken out in real time to characterize minerals, and the transformation process of the allelopathy of microorganisms on the minerals is researched; the liquid in the intermediate compartment 6 can be subjected to three-dimensional fluorescence measurement in real time to study the composition change of the liquid; the intermediate compartment 6 can be connected with an electrode to be used together with an electrochemical workstation to research the change of an electrochemical signal.
The utility model discloses a working method:
after the culture apparatus was washed, it was sterilized on a clean bench under an ultraviolet lamp for 1 hour. Selecting a filter membrane with a proper pore diameter, fixing the filter membrane between the detachable partition boards on a super clean bench, injecting the sterilized culture solution into the culture device, inoculating different kinds of algae or microorganisms into the partition boards on two sides, injecting the sterilized culture medium into the middle partition room 6, adding mineral materials, covering the cover plate 2, and sealing the vent holes 1 by using a sealing film. The culture medium is placed in an illumination shaking table for culture at the rotating speed of 120rpm, the operation is simple and convenient, and the convenience is greatly improved. And an electrode is inserted into the middle compartment 6 to detect the change of the electrochemical signal in real time, liquid is taken out to carry out real-time three-dimensional fluorescence measurement, and the product is analyzed by liquid chromatography-mass spectrometry or gas chromatography-mass spectrometry.
The above examples are merely examples for clearly illustrating the present invention and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. It is not necessary or necessary to exhaustively enumerate all embodiments herein, and obvious variations or modifications can be made without departing from the scope of the invention.
Claims (7)
1. A mineral-mediated allelochemical interaction co-cultivation device for algae and/or microorganisms, characterized in that: the culture box mainly comprises a transparent culture box, wherein two detachable partition plate devices are arranged in the middle of the transparent culture box, and each detachable partition plate device comprises two partition plates and a filter membrane clamped between the two partition plates.
2. The mineral-mediated algal and/or microbial allelochemical action co-culture apparatus of claim 1, wherein: the removable baffle device divides the transparent culture box into three compartments.
3. The mineral-mediated algal and/or microbial allelochemical action co-culture apparatus of claim 1, wherein: the detachable clapboard devices are respectively inserted into the grooves at the bottom and the side of the transparent culture box for fixing.
4. The mineral-mediated algal and/or microbial allelochemical action co-culture apparatus of claim 1, wherein: and the partition plate is provided with filter membrane holes, and the filter membrane holes are matched with filter membranes with preset apertures and sizes.
5. The mineral-mediated algal and/or microbial allelochemical action co-culture apparatus of claim 4 wherein: the filter membrane hole is equipped with the screw hole all around, the screw hole is used for fixed baffle and filter membrane.
6. The mineral-mediated algal and/or microbial allelochemical action co-culture apparatus of claim 1, wherein: a cover plate is arranged above the transparent culture box.
7. The mineral-mediated algal and/or microbial allelochemical action co-culture apparatus of claim 6, wherein: the cover plate is provided with a vent hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021982643.8U CN213624077U (en) | 2020-09-11 | 2020-09-11 | Mineral-mediated algae and/or microorganism allelopathy co-culture device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202021982643.8U CN213624077U (en) | 2020-09-11 | 2020-09-11 | Mineral-mediated algae and/or microorganism allelopathy co-culture device |
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| CN213624077U true CN213624077U (en) | 2021-07-06 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114631446A (en) * | 2022-03-31 | 2022-06-17 | 甘肃农业大学 | Allelopathy research device combining volatilization and leaching modes |
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2020
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114631446A (en) * | 2022-03-31 | 2022-06-17 | 甘肃农业大学 | Allelopathy research device combining volatilization and leaching modes |
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