CN217516923U - Novel four-hole utensil - Google Patents

Abstract

The utility model belongs to the technical field of medical appliances, a novel four-hole ware is specifically disclosed. This four-hole ware includes upper end open-ended culture dish body and can dismantle the lid and locate the ware lid of culture dish body upper end, the interval is equipped with four cultivation holes on the inside bottom surface of culture dish body, it is less than to cultivate the hole top surface the top surface of culture dish, cultivate the hole including the outer hole with locate hole in the outer hole, the inner wall of hole is the arc. The four-hole dish is convenient for observing, searching and transferring the oocyte, and meanwhile, the overflow of the culture solution can be avoided.

Description

Novel four-hole utensil

[ technical field ] A method for producing a semiconductor device

The utility model relates to the technical field of medical appliances, in particular to novel four-hole ware.

[ background of the invention ]

The intracytoplasmic sperm injection (ICSI) technology is also called second generation 'test tube baby' technology, is one of the most important advances in the auxiliary reproduction technology in recent years, is a breakthrough for treating male infertility, and is suitable for patients with extreme oligospermia and weak sperm. Before injecting the single sperm in the oocyte plasma, the granular cells at the periphery of the mature oocyte need to be removed, and the commonly adopted removal method is a hyaluronidase digestion method and a mechanical method. At present, a Nunc four-hole dish is commonly used in a reproductive center to be provided with hyaluronidase and in-vitro operating fluid as a culture dish for removing granular cells at the periphery of mature oocytes, and the specific method comprises the following steps: the ova incubated in a 5-6% CO incubator for 4-6 hours were removed, transferred to Nunc four-well dishes containing 80IU hyaluronidase/ml culture fluid (HEPES-HTF) using Pasteur pipettes with blunt nozzles for about 30-60 seconds in well 1, repeatedly blown to remove most of the cumulus cells, transferred to well 2, repeatedly blown to remove the remaining cumulus cells using STRIPPER egg breaker, transferred to HEPES-HTF-containing medium in well 3, repeatedly rinsed several times, and finally transferred to well 4 containing clean HEPES-HTF medium.

However, the four-well dish currently used has the following problems: 1. because the walls of the dish holes are vertical, the edges of the four-hole dish cannot be clearly observed under a microscope in the process of removing the oocyte, so that the time for searching the oocyte is prolonged, the later-stage embryo development is influenced, and the oocyte is even lost; 2. because the hole height in ware hole is the same with the outlying height of culture dish, consequently, at the in-process that shifts the oocyte, the novice touches the peripheral wall of pore wall or culture dish easily to lead to the glass pipe fracture, and then lead to the oocyte to lose, blow the in-process simultaneously, still make liquid overflow to operation mesa easily, cause the pollution.

[ Utility model ] content

In order to overcome prior art's not enough, the utility model aims to provide a novel four-hole ware, this novel four-hole ware overall structure is simple, convenient to use, not only is convenient for observe look for and transfer oocyte, but also can avoid the culture solution overflow, and the practicality is strong.

In order to achieve the above purpose, the utility model adopts the technical proposal that:

the utility model provides a novel four hole wares, includes upper end open-ended culture dish body and can dismantle the lid and locate the ware lid of culture dish body upper end, the culture dish body is cylindrically, the interval is equipped with four cultivation holes on the inside bottom surface of culture dish body, it is less than to cultivate the hole top surface the top surface of culture dish body, cultivate the hole including the outer hole and locating hole in the outer hole, the inner wall of hole is the arc.

Further, four of the culture wells are arranged in two rows.

Furthermore, the bottom surface of the interior of the culture dish body is provided with sequential marks in the clockwise direction corresponding to the positions of the four culture holes.

Furthermore, the two opposite sides of the outer surface of the culture dish body are provided with handles.

Furthermore, supporting legs are arranged on the bottom surface of the outer portion of the culture dish body.

Owing to used the technical scheme of the utility model, the utility model discloses following beneficial effect has:

the utility model discloses a four-hole ware sets the arc to through the inner wall with the hole, make the hole be the bowl form that from the top down closed up gradually around the space that forms, not only be convenient for the microscope clearly observe the hole edge, thereby seek oocyte fast, and can reduce the quantity of culture solution on the basis of fully soaking oocyte, reduce extravagantly, and set up the outer hole outside the hole, both are around forming the dashpot, can effectively receive the culture solution that blows the in-process overflow and come out, avoid the culture solution to spatter outward, the overflow goes out the culture dish body, meanwhile, the setting that highly is less than culture dish body height in culture hole, can avoid glass pipe and culture dish body collision when oocyte shifts, improve the security that oocyte shifted.

[ description of the drawings ]

Fig. 1 is a schematic structural view of the four-hole dish of the present invention after the dish lid is removed.

Fig. 2 is a top view of fig. 1.

Fig. 3 is a cross-sectional view of fig. 1.

Figure 4 is a schematic view of the capsule of figure 1 after addition of the capsule.

Fig. 5 is a schematic structural view of another four-well dish according to the present invention.

The main reference symbols in the drawings are as follows:

in the figure, 1-culture dish body, 2-culture hole, 21-outer hole, 22-inner hole, 3-sequence label, 4-dish cover, 5-handle, 6-supporting foot.

The following detailed description of the invention will be further described in conjunction with the above-identified drawings.

[ detailed description ] embodiments

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

As shown in fig. 1-4, in a preferred embodiment of the present invention, a novel four-hole dish comprises a culture dish body 1 with an open upper end and a detachable cover, wherein the culture dish cover 4 is disposed on the upper end of the culture dish body 1, the culture dish cover 4 and the culture dish body 1 can be connected by a direct cover or a card, four culture holes 2 are disposed on the bottom surface inside the culture dish body 1 at intervals, two rows of the culture holes 2 are disposed, and the bottom surface inside the culture dish body 1 corresponds to four positions of the culture holes 2 are provided with sequence marks 3 in a clockwise direction, i.e. marks 1, 2, 3 and 4, so that an operator can more clearly grasp the moving sequence of oocytes. The top surface of the culture hole 2 is lower than the top surface of the culture dish body 1, namely the height of the culture hole 2 is lower than the height of the culture dish body 1, so that the phenomenon that the oocyte is lost due to collision of the side wall of the culture dish body 1 with the glass tube when the oocyte is transferred is avoided, and the safety of transferring the oocyte is improved. Cultivate hole 2 includes outer hole 21 and locates hole 22 in the outer hole 21, outer hole 21 the bottom surface of culture dish body 1 with hole 22 is around forming the dashpot for receive the follow the culture solution that hole 22 overflowed out avoids the culture solution to splash and overflows outside culture dish body 1, the inner wall of hole 22 is the arc, promptly hole 22 with the bottom surface of culture dish body 1 is open bowl form around the space that forms, is convenient for on the one hand clear observation under the microscope seeks the oocyte, and on the other hand can reduce the quantity of culture solution on the basis of fully soaking the oocyte.

Referring to fig. 5, in another embodiment of the present invention, the two opposite sides of the outer surface of the culture dish body 1 are respectively provided with a handle 5, so as to facilitate the movement of the culture dish body 1, and the support legs 6 are arranged on the bottom surface of the outer portion of the culture dish body 1, the support legs 6 are 4, and the support legs 6 are uniformly distributed, so as to facilitate the lifting of the culture dish body 1.

To sum up, the utility model discloses a novel four hole ware overall structure is simple, convenient to use, is convenient for not only observe to look for and transfer oocyte, but also can avoid the culture solution overflow, and the practicality is strong.

Claims (5)

1. The utility model provides a novel four-hole ware, includes upper end open-ended culture dish body and can dismantle the lid and locate the ware lid of culture dish body upper end, its characterized in that: four culture holes are arranged on the bottom surface inside the culture dish body at intervals, the top surfaces of the culture holes are lower than the top surface of the culture dish body, each culture hole comprises an outer hole and an inner hole arranged in the outer hole, and the inner wall of each inner hole is arc-shaped.

2. The novel four-well dish of claim 1, wherein: the four culture wells are arranged in two rows.

3. A novel four-well dish according to claim 2, wherein: and sequential labels are arranged at the positions of the bottom surface in the culture dish body, which correspond to the four culture holes, in the clockwise direction.

4. The novel four-well dish of claim 1, wherein: handles are arranged on two opposite sides of the outer surface of the culture dish body.

5. The novel four-well dish of claim 1, wherein: supporting legs are arranged on the bottom surface of the outer portion of the culture dish body.

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