CN209081870U - One kind is novel to pick up egg apparatus and oocytes collection device - Google Patents

One kind is novel to pick up egg apparatus and oocytes collection device Download PDF

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Publication number
CN209081870U
CN209081870U CN201821577004.6U CN201821577004U CN209081870U CN 209081870 U CN209081870 U CN 209081870U CN 201821577004 U CN201821577004 U CN 201821577004U CN 209081870 U CN209081870 U CN 209081870U
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China
Prior art keywords
barrier wall
layer
layer barrier
egg apparatus
filter layer
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Expired - Fee Related
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CN201821577004.6U
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Chinese (zh)
Inventor
李晓霞
刁云飞
朱海瑛
许保增
王士勇
魏海军
张宇飞
李丹丽
常彤
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Institute Special Animal and Plant Sciences CAAS
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Institute Special Animal and Plant Sciences CAAS
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Priority to CN201821577004.6U priority Critical patent/CN209081870U/en
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Abstract

The utility model provide it is a kind of it is novel pick up egg apparatus and oocytes collection device, belong to biological study technical field.It includes vessel ontology, first layer barrier wall and the first filter layer;The rounded uncovered structure of vessel ontology, vessel ontology have bottom plate, offer the first mounting groove, the first mounting groove structure in rounded shape on bottom plate;First layer barrier wall structure annular in shape, first layer barrier wall are removably mounted in the first mounting groove;First filter layer is removably mounted on the inner wall of first layer barrier wall, and the outer diameter of the first filter layer is identical as the internal diameter of first layer barrier wall.The oocytes collection device, structure are simple and easy to use.

Description

One kind is novel to pick up egg apparatus and oocytes collection device
Technical field
The utility model relates to biological study technical fields, pick up egg apparatus and egg mother cell in particular to one kind is novel Collection device.
Background technique
Embryo engineering refers to a variety of micromanipulations carried out to animal body early embryo or gamete and processing technique, including body Technologies, the immature ovums of in vitro culture such as outer fertilization, embryo transfer, embryo separating transplanting, embryonic stem cell culture are embryo's works More important technology in journey is that immature ovum is cultivated maturation in vitro, is then fertilized, develops into embryo, then be implanted into The characteristics of maternal uterine, Oocytes Maturation technology is to be in the very immature stage in ovum just to take out from internal, and in body Culture becomes mature ovum under the specified conditions of outer culture dish.Embryo Culture process is that ovum is placed in embryo medium to make Its growing, as being rich in embryo medium for various amino acid required for embryonic development, but embryo was cultivating Continuous variation occurs for Cheng Zhongqi physiology and metabolism, and ammonia caused by amino acid decomposition and embryo metabolism is to the toxic work of embryo With, it is therefore desirable to successional offer embryo medium simultaneously replaces waste liquid simultaneously.
Replacement embryo medium is that culture solution is drawn by artificial aspirator at present, inconvenient, also will cause embryo and loses It loses.
Utility model content
The utility model, which provides, a kind of novel picks up egg apparatus, it is intended to which solution is picked up inconvenient existing for egg apparatus in the prior art Prompt problem;
The utility model provides a kind of oocytes collection device, it is intended to which the existing behaviour of egg apparatus is picked up in solution in the prior art Make inconvenient problem.
The utility model embodiment is achieved in that
One kind is novel to pick up egg apparatus, and the novel egg apparatus of picking up includes vessel ontology, first layer barrier wall and the first filter layer;
The rounded uncovered structure of vessel ontology, the vessel ontology have bottom plate, offer first on the bottom plate Mounting groove, the first mounting groove structure in rounded shape;
The first layer barrier wall structure annular in shape, the first layer barrier wall are removably mounted on first peace In tankage, the height of the vessel ontology is greater than the height of the first layer barrier wall;
First filter layer is removably mounted on the inner wall of the first layer barrier wall, first filter layer Outer diameter is identical as the internal diameter of the first layer barrier wall.
As a kind of optimal technical scheme of the utility model, at least two positioning is offered on the first layer barrier wall Mouthful, the locating rod that at least two is equipped with the positioning port is installed, first filter layer is logical on first filter layer Cross the locating rod is mounted on the positioning port it is detachably connected with the first layer barrier wall.
As a kind of optimal technical scheme of the utility model, the positioning port is 4, the spacing between the positioning port It is identical and be circumferentially disposed on the first layer barrier wall;The locating rod is 4, and the spacing between the locating rod is identical And it is circumferentially disposed on first filter layer.
As a kind of optimal technical scheme of the utility model, olecranon is offered on the first layer barrier wall.
As a kind of optimal technical scheme of the utility model, the first layer barrier wall and the first mounting groove Tenon Connection is threadedly coupled.
As a kind of optimal technical scheme of the utility model, the net diameter of first filter layer is 50~110um.
As a kind of optimal technical scheme of the utility model, the novel egg apparatus of picking up includes second layer barrier wall and second Filter layer;The second mounting groove, the second mounting groove structure in rounded shape, the second layer barrier wall are offered on the bottom plate Structure annular in shape, the second layer barrier wall are removably mounted in second mounting groove;Second filter layer can It is releasably socketed on the outer wall of the first layer barrier wall, and connects with the inner wall of the second layer barrier wall;Described second Layer barrier wall is between the first layer barrier wall and the gap of the vessel ontology, the vessel ontology, the second layer The height of barrier wall and the first layer barrier wall is sequentially reduced.
As a kind of optimal technical scheme of the utility model, the second layer barrier wall and the second mounting groove Tenon Connection is threadedly coupled.
As a kind of optimal technical scheme of the utility model, the net diameter of second filter layer is 50~110um.
A kind of oocytes collection device, the oocytes collection device include above-described novel picking up egg apparatus.
The beneficial effects of the utility model are:
The utility model, which provides, a kind of novel picks up egg apparatus comprising vessel ontology, first layer barrier wall and the first filtering Layer;Due to first layer barrier wall structure annular in shape, first layer barrier wall is removably mounted in the first mounting groove;Therefore, In use, since the present apparatus is provided with first layer barrier wall in vessel ontology, between first layer barrier wall and vessel ontology just With gap, and therefore on the one hand the height of vessel ontology ensure that sealing effect, energy greater than the height of first layer barrier wall It is enough effectively prevented from culture solution spilling, meanwhile, eagle mouth structure is provided on first layer barrier wall, embryo medium can pass through Eagle mouth structure is toppled over so that culture solution topple over it is more smooth and conveniently;In addition, the first filter layer is removably mounted on first On the inner wall of layer barrier wall, the net diameter of the first filter layer is 50~110um, therefore, when embryo medium is poured over the first filtering When on layer, since the diameter of ovum is all in 120um or more, and the diameter of the haemocyte in liquor folliculi in 20um hereinafter, therefore ovum Son is found on the vessel ontology of first filter layer after taking out, after ovum has all been picked up, by first filter layer from vessel Lift on ontology, then haemocyte is all omitted in original culture solution, and ovum stays on the first filter layer, and ovum will be housed First filter layer, which is transferred to another and has in the culture dish of culture solution, to be washed, by so several times washing and then will Ovum picking one by one, does not have haemocyte then at this time and mixes ovum culture solution and suffer.Meanwhile first filter layer pass through by Locating rod is mounted on positioning port detachably connected with first layer barrier wall, and therefore, which can easily facilitate first The transfer of filter layer.Further, since offering olecranon on first layer barrier wall, therefore, when toppling over culture solution, can be set Toppled on olecranon, it is more convenient to operate in this way, and can prevent liquid leakage.Also, due to first layer every The time that retaining wall uses is longer, is easy to produce some residues and can pick up ovum work to subsequent and bring inconvenience, therefore, Ke Yitong Cross the first layer barrier wall that first layer barrier wall is disassembled from the first mounting groove and is more renewed, this kind of mortise-tenon joint or The mode of threaded connection is more convenient for dismantlement work.Secondly, novel egg apparatus of picking up includes second layer barrier wall and the second mistake Filtering layer;Offer the second mounting groove on bottom plate, the second mounting groove structure in rounded shape, second layer barrier wall structure annular in shape, Second layer barrier wall is removably mounted in the second mounting groove;Therefore, which can effectively further prevent Only intravenous extravasation, and pass through the filtering of second filter layer, more efficiently ovum can be screened.
The oocytes collection device that the utility model obtains by above-mentioned design, the oocytes collection device is due to tool Have it is above it is novel pick up egg apparatus, by being screened in novel pick up in egg apparatus to the ovum in culture solution, on the one hand can prevent Only liquid leakage, on the other hand can effectively improve the efficiency of screening, and structure is simple and easy to operate.
Detailed description of the invention
It, below will be to required in embodiment in order to illustrate more clearly of the technical solution of the utility model embodiment The attached drawing used is briefly described, it should be understood that the following drawings illustrates only some embodiments of the utility model, therefore not It should be considered as the restriction to range, for those of ordinary skill in the art, without creative efforts, It can also be obtained according to these attached drawings other relevant attached drawings.
Fig. 1 is provided by the embodiment of the utility model novel to pick up egg apparatus structural schematic diagram;
Fig. 2 is provided by the embodiment of the utility model novel to pick up egg apparatus first angle structural schematic diagram;
Fig. 3 is the first filter layer provided by the embodiment of the utility model and first layer barrier wall attachment structure schematic diagram;
Fig. 4 is the second filter layer provided by the embodiment of the utility model and second layer barrier wall attachment structure schematic diagram.
Icon: 1- is novel to pick up egg apparatus;2- vessel ontology;3- first layer barrier wall;The first filter layer of 4-;5- bottom plate;6- One mounting groove;7- positioning port;8- locating rod;10- olecranon;11- second layer barrier wall;The second filter layer of 12-;13- second is installed Slot.
Specific embodiment
It is practical below in conjunction with this to keep the purposes, technical schemes and advantages of the utility model embodiment clearer The technical solution in the utility model embodiment is clearly and completely described in attached drawing in novel embodiment, shows So, described embodiment is a part of embodiment of the utility model, rather than whole embodiments.It is practical based on this Embodiment in novel, it is obtained by those of ordinary skill in the art without making creative efforts every other Embodiment is fallen within the protection scope of the utility model.Therefore, the implementation to the utility model provided in the accompanying drawings below The detailed description of mode is not intended to limit the range of claimed invention, but is merely representative of the utility model Selected embodiment.
In the description of the present invention, it should be understood that indicating position or the term of positional relationship are based on attached drawing Shown in orientation or positional relationship, be merely for convenience of describing the present invention and simplifying the description, rather than indication or suggestion institute The element of finger must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as to the utility model Limitation.
In the present invention unless specifically defined or limited otherwise, term " installation ", " connected ", " connection ", " Gu It is fixed " etc. terms shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integral;It can be Mechanical connection, is also possible to be electrically connected;It can be directly connected, two can also be can be indirectly connected through an intermediary The interaction relationship of connection or two elements inside element.It for the ordinary skill in the art, can basis Concrete condition understands the concrete meaning of above-mentioned term in the present invention.
In the present invention unless specifically defined or limited otherwise, fisrt feature is on or below second feature It may include that the first and second features directly contact, also may include that the first and second features are not direct contacts but pass through it Between other characterisation contact.Moreover, fisrt feature is on second feature, top and above include fisrt feature the Right above two features and oblique upper, or first feature horizontal height is merely representative of higher than second feature.Fisrt feature is in the second spy Under sign, lower section and fisrt feature included below be directly below and diagonally below the second feature, or be merely representative of fisrt feature level Height is less than second feature.
Embodiment:
A kind of oocytes collection device is present embodiments provided, is mainly used for taking out the ovum in animal ovary.
Oocytes collection device include it is novel pick up egg apparatus 1, pass through this it is novel pick up egg apparatus 1 take out animal ovary in ovum Son, so as to the research or treatment etc. of various bioscience.
Referring to Fig. 1, cooperation referring to Figure 2 to Figure 4, it is novel pick up egg apparatus 1 include vessel ontology 2, first layer barrier wall 3 and First filter layer 4;The rounded uncovered structure of vessel ontology 2, vessel ontology 2 have bottom plate 5, offer the first installation on bottom plate 5 Slot 6, the structure in rounded shape of the first mounting groove 6;The structure annular in shape of first layer barrier wall 3, first layer barrier wall 3 are removably pacified In the first mounting groove 6;First filter layer 4 is removably mounted on the inner wall of first layer barrier wall 3, the first filter layer 4 Outer diameter it is identical as the internal diameter of first layer barrier wall 3.
Therefore, in use, since the present apparatus is provided with first layer barrier wall 3, first layer barrier wall 3 in vessel ontology 2 Just there is gap between vessel ontology 2, and the height of vessel ontology 2 is greater than the height of first layer barrier wall 3, therefore, a side Face ensure that sealing effect, can be effectively prevented from culture solution spilling.
Referring to Fig. 3, cooperation is referring to Fig.1,4 positioning ports 7 are offered on first layer barrier wall 3, are pacified on the first filter layer 4 Equipped with 4 locating rods being equipped with positioning port 78, the first filter layer 4 is by the way that locating rod 8 to be mounted on positioning port 7 and One layer of barrier wall 3 is detachably connected.
It should be noted that in the present embodiment, the spacing between positioning port 7 it is identical and be circumferentially disposed at first layer every On retaining wall 3;Locating rod 8 is 4, and the spacing between locating rod 8 is identical and is circumferentially disposed on the first filter layer 4.Therefore, One filter layer 4 is detachably connected with first layer barrier wall 3 by the way that locating rod 8 to be mounted on positioning port 7, therefore, the positioning Bar 8 can easily facilitate the transfer of the first filter layer 4.
It should be noted that in the present embodiment, offering olecranon 10 on first layer barrier wall 3.Therefore, when topple over culture When liquid, it can place it on olecranon 10 and be toppled over, it is more convenient to operate in this way, and can prevent liquid leakage.
It should be noted that in the present embodiment, first layer barrier wall 3 and 6 mortise-tenon joint of the first mounting groove or screw thread Connection.Since the time that first layer barrier wall 3 uses is longer, it is easy to produce some residues and can picks up ovum work to subsequent and make At inconvenience, therefore, the first layer that can be more renewed and disassembling first layer barrier wall 3 from the first mounting groove 6 every The mode of retaining wall 3, this kind of mortise-tenon joint or threaded connection is more convenient for dismantlement work.
It should be noted that in the present embodiment, the net diameter of the first filter layer 4 is 50~110um.Therefore, when embryo trains When nutrient solution is poured on the first filter layer 4, since the diameter of ovum is all in 120um or more, and the haemocyte in liquor folliculi is straight Diameter in 20um hereinafter, therefore ovum take out after find on the vessel ontology 2 of first filter layer 4, after ovum has all been picked up, First filter layer 4 is lifted from vessel ontology 2, then haemocyte is all omitted in original culture solution, and ovum stays in the On one filter layer 4, the first filter layer 4 equipped with ovum is transferred to another and had in the culture dish of culture solution and is washed, is passed through It crosses washing several times in this way and then the picking one by one by ovum, does not have haemocyte then at this time and mix ovum culture solution It suffers.
Referring to Fig. 4, cooperation is referring to Fig.1, novel egg apparatus 1 of picking up includes second layer barrier wall 11 and the second filter layer 12;Bottom Offer the second mounting groove 13 on plate 5, the structure in rounded shape of the second mounting groove 13, the structure annular in shape of second layer barrier wall 11, Second layer barrier wall 11 is removably mounted in the second mounting groove 13;Second filter layer 12 be removably socketed in first layer every On the outer wall of retaining wall 3, and connect with the inner wall of second layer barrier wall 11;Second layer barrier wall 11 is in 3 He of first layer barrier wall Between the gap of vessel ontology 2.
It should be noted that in the present embodiment, second layer barrier wall 11 and 13 mortise-tenon joint of the second mounting groove or spiral shell Line connection.
It should be noted that in the present embodiment, the net diameter of the second filter layer 12 is 50~110um.
In conclusion novel egg apparatus 1 of picking up includes vessel ontology 2, first layer barrier wall 3 and the first filter layer 4;Due to One layer of structure annular in shape of barrier wall 3, first layer barrier wall 3 are removably mounted in the first mounting groove 6;Therefore, in use, Since the present apparatus is provided with first layer barrier wall 3 in vessel ontology 2, just have between first layer barrier wall 3 and vessel ontology 2 Gap, and therefore on the one hand the height of vessel ontology 2 ensure that sealing effect greater than the height of first layer barrier wall 3, it can It is effectively prevented from culture solution spilling, meanwhile, it is provided with 10 structure of olecranon on first layer barrier wall 3, embryo medium can be through 10 structure of olecranon is crossed to topple over so that culture solution topple over it is more smooth and conveniently;In addition, the first filter layer 4 is removably installed On the inner wall of first layer barrier wall 3, the net diameter of the first filter layer 4 is 50~110um, therefore, when embryo medium is poured over When on the first filter layer 4, since the diameter of ovum is all in 120um or more, and the diameter of the haemocyte in liquor folliculi 20um with Under, therefore found on the vessel ontology 2 of first filter layer 4 after ovum taking-up, after ovum has all been picked up, by first mistake Filtering layer 4 lifts from vessel ontology 2, then haemocyte is all omitted in original culture solution, and ovum stays in the first filter layer 4 On, the first filter layer 4 equipped with ovum is transferred to another and had in the culture dish of culture solution and is washed, by so several times Washing and then the picking one by one by ovum, do not have haemocyte then at this time and mix ovum culture solution and suffer.Meanwhile First filter layer 4 is detachably connected with first layer barrier wall 3 by the way that locating rod 8 to be mounted on positioning port 7, and therefore, this is fixed Position bar 8 can easily facilitate the transfer of the first filter layer 4.Further, since olecranon 10 is offered on first layer barrier wall 3, therefore, When toppling over culture solution, it can place it on olecranon 10 and be toppled over, it is more convenient to operate in this way, and can prevent Liquid leakage.Also, since the time that first layer barrier wall 3 uses is longer, be easy to produce some residues and can be to subsequent It picks up ovum work to bring inconvenience, therefore, can more be renewed and disassembling first layer barrier wall 3 from the first mounting groove 6 First layer barrier wall 3, the mode of this kind of mortise-tenon joint or threaded connection is more convenient for dismantlement work.Secondly, new It includes second layer barrier wall 11 and the second filter layer 12 that type, which picks up egg apparatus 1,;The second mounting groove 13, the second installation are offered on bottom plate 5 The structure in rounded shape of slot 13, the structure annular in shape of second layer barrier wall 11, second layer barrier wall 11 are removably mounted on second In mounting groove 13;Therefore, which can effectively further prevent intravenous extravasation, and pass through second mistake The filtering of filtering layer 12 can more efficiently screen ovum.The oocytes collection device due to having more than It is novel pick up egg apparatus 1, by screening in novel pick up in egg apparatus 1 to the ovum in culture solution, on the one hand can prevent liquid External leakage, on the other hand can effectively improve the efficiency of screening, structure is simple and easy to operate.
Above description is merely a prefered embodiment of the utility model, is not intended to limit the utility model, for For those skilled in the art, various modifications and changes may be made to the present invention.All spirit and original in the utility model Within then, any modification, equivalent replacement, improvement and so on be should be included within the scope of protection of this utility model.

Claims (10)

1. novel egg apparatus is picked up a kind of, which is characterized in that the novel egg apparatus of picking up includes vessel ontology, first layer barrier wall and the One filter layer;
The rounded uncovered structure of vessel ontology, the vessel ontology have bottom plate, offer the first installation on the bottom plate Slot, the first mounting groove structure in rounded shape;
The first layer barrier wall structure annular in shape, the first layer barrier wall are removably mounted on first mounting groove Interior, the height of the vessel ontology is greater than the height of the first layer barrier wall;
First filter layer is removably mounted on the inner wall of the first layer barrier wall, the outer diameter of first filter layer It is identical as the internal diameter of the first layer barrier wall.
2. novel egg apparatus is picked up according to claim 1, which is characterized in that offers at least 2 on the first layer barrier wall A positioning port is equipped at least two on first filter layer and locating rod that the positioning port is equipped with, first mistake Filtering layer is detachably connected with the first layer barrier wall by the way that the locating rod to be mounted on the positioning port.
3. novel egg apparatus is picked up according to claim 2, which is characterized in that the positioning port is 4, between the positioning port Spacing it is identical and be circumferentially disposed on the first layer barrier wall;The locating rod is 4, between the locating rod between Away from identical and be circumferentially disposed on first filter layer.
4. novel egg apparatus is picked up according to claim 1, which is characterized in that offers olecranon on the first layer barrier wall.
5. novel egg apparatus is picked up according to claim 1, which is characterized in that the first layer barrier wall and first installation Slot mortise-tenon joint or threaded connection.
6. novel egg apparatus is picked up according to claim 1, which is characterized in that the net diameter of first filter layer is 50~ 110um。
7. novel egg apparatus is picked up according to claim 1, which is characterized in that the novel egg apparatus of picking up includes second layer barrier wall With the second filter layer;The second mounting groove, the second mounting groove structure in rounded shape, the second layer are offered on the bottom plate Barrier wall structure annular in shape, the second layer barrier wall are removably mounted in second mounting groove;Second mistake Filtering layer is removably socketed on the outer wall of the first layer barrier wall, and is connected with the inner wall of the second layer barrier wall;Institute Second layer barrier wall is stated to be between the first layer barrier wall and the gap of the vessel ontology, it is the vessel ontology, described The height of second layer barrier wall and the first layer barrier wall is sequentially reduced.
8. novel egg apparatus is picked up according to claim 7, which is characterized in that the second layer barrier wall and second installation Slot mortise-tenon joint or threaded connection.
9. novel egg apparatus is picked up according to claim 8, which is characterized in that the net diameter of second filter layer is 50~ 110um。
10. a kind of oocytes collection device, which is characterized in that the oocytes collection device includes that claim 1-9 appoints It is novel described in one to pick up egg apparatus.
CN201821577004.6U 2018-09-26 2018-09-26 One kind is novel to pick up egg apparatus and oocytes collection device Expired - Fee Related CN209081870U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201821577004.6U CN209081870U (en) 2018-09-26 2018-09-26 One kind is novel to pick up egg apparatus and oocytes collection device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201821577004.6U CN209081870U (en) 2018-09-26 2018-09-26 One kind is novel to pick up egg apparatus and oocytes collection device

Publications (1)

Publication Number Publication Date
CN209081870U true CN209081870U (en) 2019-07-09

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CN201821577004.6U Expired - Fee Related CN209081870U (en) 2018-09-26 2018-09-26 One kind is novel to pick up egg apparatus and oocytes collection device

Country Status (1)

Country Link
CN (1) CN209081870U (en)

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Granted publication date: 20190709

Termination date: 20190926