CN217487208U - Tweezers for explant sterile inoculation - Google Patents
Tweezers for explant sterile inoculation Download PDFInfo
- Publication number
- CN217487208U CN217487208U CN202221809191.2U CN202221809191U CN217487208U CN 217487208 U CN217487208 U CN 217487208U CN 202221809191 U CN202221809191 U CN 202221809191U CN 217487208 U CN217487208 U CN 217487208U
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- tweezers
- plug
- forceps
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- inoculation
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- 238000011081 inoculation Methods 0.000 title claims abstract description 33
- 239000007788 liquid Substances 0.000 claims description 7
- 230000008878 coupling Effects 0.000 claims description 4
- 238000010168 coupling process Methods 0.000 claims description 4
- 238000005859 coupling reaction Methods 0.000 claims description 4
- 230000000903 blocking effect Effects 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 230000007704 transition Effects 0.000 claims description 3
- 230000006872 improvement Effects 0.000 abstract description 4
- 238000004166 bioassay Methods 0.000 abstract description 2
- 239000000463 material Substances 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- 238000011109 contamination Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229930195732 phytohormone Natural products 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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Abstract
The utility model discloses a tweezers are used in aseptic inoculation of explant relates to biological assay utensil technical field. The tweezers comprise tweezers, the heads of two tweezers arms of the tweezers are provided with plugs, disposable plugs are inserted into the plugs in a plugging manner, push heads used for pushing the disposable plugs away from the plugs are arranged on the plugs in a sliding manner, and driving components used for driving the push heads to slide are arranged on the tweezers arms. The utility model discloses, use same tweezers to carry out explant inoculation or aseptic transfer, the head of tweezers can be dismantled and be provided with disposable plug, carries out explant inoculation or aseptic transfer through disposable plug, can dismantle the disposable plug of more renewing fast, and is simple swift, improvement inoculation or aseptic transfer efficiency that can be very big.
Description
Technical Field
The utility model relates to a biological assay utensil technical field specifically is a tweezers are used in aseptic inoculation of explant.
Background
Explant inoculation is one of the important links of plant tissue culture, and refers to a technology of inoculating a sterile explant to a culture medium containing various nutrient substances and phytohormones for culture and obtaining a regenerated complete plant or other products with economic value by utilizing totipotency of cells. Usually, the explant is inoculated to a seedling, and the seedling is required to undergo the processes of inoculation-induction culture-subculture-differentiation culture and the like. In these processes, the nonstandard disinfection of the instruments or technical operation can cause the culture medium or culture materials to grow microorganisms such as bacteria and fungi, and the culture materials cannot grow and develop normally, so that the loss is caused.
At present, inoculation or sterile transfer of explants is usually achieved using common forceps. Generally, there are two main ways of sterilizing ordinary forceps. One is to sterilize the tweezers with alcohol before inoculation and put the tweezers into a clean bench, burn the tips of the tweezers on an alcohol lamp to turn red, and then inoculate the explants after the tips of the tweezers are cooled. This operation takes about 5 minutes to complete, and in order to reduce the contamination rate, the operation needs to be repeated for transferring each external dish, which is time-consuming and seriously reduces the transfer efficiency. Also, forceps that are normally burned red can cause damage to the explant if not cooled completely, thereby increasing the damage to the explant and resulting in waste. In another method, after the tweezers are sterilized at high temperature, the aseptic tweezers are directly used during explant inoculation, but when the explant inoculation amount is larger, a large amount of aseptic tweezers are needed to meet the requirement, the experiment cost is increased, and the inoculation efficiency is reduced by frequently replacing the aseptic tweezers.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a tweezers are used in aseptic inoculation of explant uses same tweezers to carry out the explant inoculation or aseptic transfer, and the head of tweezers can be dismantled and be provided with disposable plug, carries out the explant inoculation or aseptic transfer through disposable plug, can dismantle the disposable plug of renewal fast, and simple swift, improvement inoculation or aseptic transfer efficiency that can be very big.
In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a tweezers are used in aseptic inoculation of explant, includes tweezers, the head of two tweezers arms of tweezers is provided with the plug, peg graft disposable plug on the plug, it is used for pushing away disposable plug to slide on the plug the pushing head of plug, be provided with on the tweezers arm and be used for the drive the gliding drive assembly of pushing head.
Preferably, the disposable plug is a liquid transfer pipette tip, and the plug is arranged into a cylindrical structure matched with the liquid transfer pipette tip.
Preferably, the driving assembly comprises two sliding seats which are respectively arranged on the forceps arms in a sliding manner, the sliding seats are in transition connection with the corresponding pushing heads through connecting rods, a first connecting plate and a second connecting plate are respectively arranged on the inner sides of the two sliding seats, and the first connecting plate and the second connecting plate are in sliding connection.
Preferably, the first connecting plate is provided with a connecting raised head, the second connecting plate is provided with a guide groove matched with the connecting raised head, the guide groove is arc-shaped, and the circle center of the guide groove is overlapped with the rotating connection point of the two forceps arms.
Preferably, one side of plug is provided with dodges the mouth, the centre of a circle department of pushing away the head just is located the inside of plug be provided with push away first transitional coupling's connecting portion, the pushing away the head pass through connecting portion with connecting rod transitional coupling.
Preferably, an elastic element for blocking the sliding seat from moving towards the head of the forceps arm is arranged between one sliding seat and the forceps arm.
Preferably, a fixing seat is arranged on one forceps arm, a driving rod penetrating through the fixing seat is arranged on a corresponding sliding seat on the forceps arm, a pressing plate is arranged at the suspended end of the driving rod, and the elastic part is a spring which is sleeved outside the driving rod and is located between the fixing seat and the pressing plate.
Compared with the prior art, the beneficial effects of the utility model are that:
1. the utility model discloses, use same tweezers to carry out explant inoculation or aseptic transfer, the head of tweezers can be dismantled and be provided with disposable plug, carries out explant inoculation or aseptic transfer through disposable plug, can dismantle the disposable plug of more renewing fast, and is simple swift, improvement inoculation or aseptic transfer efficiency that can be very big, does not have the drawback of high temperature damage explant simultaneously.
2. The utility model discloses, through the chuck of liquid-transfering suction head as tweezers, liquid-transfering suction head is disposable chuck promptly, liquid-transfering suction head belongs to the consumptive material commonly used in laboratory, and the low price, can high temperature sterilization. Therefore, the suction head box with the liquid-transfering suction head is sterilized at high temperature, and the tweezers of the design do not need sterilization. And 96 liquid-transfering suction heads can be installed and taken out from one gun head box, 48 times of suction head replacement can be realized, one time of suction head replacement can be realized, and the risk of contamination is greatly reduced.
3. The utility model discloses, occupation space is little, and is with low costs, can realize that the aseptic suction head of high frequency change just takes time fewly. And the configured suction head box and the suction head are high-frequency consumable materials for laboratories, special customization is not needed, the cost is low, and the popularization and the application are convenient.
Drawings
Fig. 1 is a first schematic structural diagram of the present invention;
FIG. 2 is a second schematic structural view of the present invention;
FIG. 3 is an enlarged view of a portion A of FIG. 2 according to the present invention;
fig. 4 is a schematic structural diagram of the plug of the present invention;
fig. 5 is a schematic structural view of the pushing head of the present invention.
In the figure:
1-forceps arm, 11-plug, 111-avoidance port, 12-fixing seat,
2-a pushing head, 21-a connecting part,
3-a spring is arranged in the groove,
4-a connecting rod is arranged at the upper part of the frame,
5-a sliding seat which is provided with a sliding seat,
61-first connecting plate, 611-connecting projection, 62-second connecting plate, 621-guide groove,
7-a driving rod, 71-a pressing plate,
8-pipetting tip.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
As shown in fig. 1 to 5, the forceps for explant sterile inoculation comprises forceps, wherein plugs 11 are arranged at the heads of two forceps arms 1 of the forceps, a disposable plug is inserted into the plugs 11, a pushing head 2 for pushing the disposable plug away from the plugs 11 is slidably arranged on the plugs 11, and a driving component for driving the pushing head 2 to slide is arranged on the forceps arms 1.
As an embodiment, the disposable plug is a pipette tip 8, as shown in fig. 1-2. In the embodiment, the pipette tip 8 adopts a 96-hole gun tip box and a common standard pipette tip (0.5-10 μ l), and the design is designed to: the pipette tip is used as a clamping head of the tweezers, belongs to a consumable material commonly used in a laboratory, is low in price and can be sterilized at high temperature. Therefore, the pipette tip box provided with the pipette tip is sterilized at high temperature, and the tweezers of the design do not need sterilization. And 96 liquid-transfering suction heads can be installed and taken out from one gun head box, 48 times of suction head replacement can be realized, one time of suction head replacement can be realized, and the risk of contamination is greatly reduced.
As a specific embodiment, as shown in fig. 4 to 5, the plug 11 is configured as a cylindrical structure to which the pipette tip 8 can be inserted, and the pusher 2 is configured as an annular structure and is slidably disposed outside the plug 11.
As a specific embodiment, as shown in fig. 1 to 3, the driving assembly includes two sliding seats 5 respectively slidably disposed on the two forceps arms 1, the sliding seats 5 are transitionally connected with the corresponding pushing heads 2 through a connecting rod 4, a first connecting plate 61 and a second connecting plate 62 are respectively disposed on inner sides of the two sliding seats 5 (taking a side of the two sliding seats 5 opposite to each other as an inner side), and the first connecting plate 61 and the second connecting plate 62 are slidably connected with each other. Thus, while driving one of the carriages 5 up and down, the other carriage 5 is simultaneously moving. When the plug 11 is plugged with the liquid transfer pipette tips 8, the two liquid transfer pipette tips 8 can be pushed away from the push head 2 of the plug 11 by moving the two sliders 5 and pushing the push head 2 to move towards the liquid transfer pipette tips 8 through the two connecting rods 4.
Specifically, in this embodiment, as shown in fig. 3, the first connecting plate 61 is provided with a connecting protruding head 611, the second connecting plate 62 is provided with a guide groove 621 matching with the connecting protruding head 611, the guide groove 621 is set to be arc-shaped, and the center of the guide groove 621 coincides with the rotational connection point of the two forceps arms 1, and the design is as follows: firstly, the transition connection between the two sliding seats 5 is realized through the first connecting plate 61 and the second connecting plate 62, so that the other sliding seat 5 can synchronously move while one sliding seat 5 is driven to move; secondly, when the two forceps arms 1 are driven to close, the two sliding bases 5 can move along with the movement generated by the closing of the forceps arms 1 through the sliding guiding action of the connecting convex heads 611 and the guide grooves 621.
Specifically, in this embodiment, as shown in fig. 4 to 5, an avoiding opening 111 is provided at one side of the plug 11, a connecting portion 21 transitionally connected with the pushing head 2 is provided at the center of the circle of the pushing head 2 and inside the plug 11, and the pushing head 2 is transitionally connected with the connecting rod 4 through the connecting portion 21. Therefore, the stress point of the push head 2 is pushed by the connecting rod 4 to be in the central part of the push head 2, so that the push head 2 is driven to move conveniently.
Further, an elastic member for blocking the sliding base 5 from moving towards the head of the forceps arm 1 is arranged between one or both sliding bases 5 and the forceps arm 1.
Specifically, in this embodiment, one elastic member is adopted, as shown in fig. 3, a fixing seat 12 is arranged on one forceps arm 1, a driving rod 7 penetrating through the fixing seat 12 is arranged on a corresponding sliding seat 5 on the forceps arm 1, a pressing plate 71 is arranged at a suspended end of the driving rod 7, and the elastic member is a spring 3 which is sleeved outside the driving rod 7 and is located between the fixing seat 12 and the pressing plate 71. Thus, under the action of the spring force of the spring 3, a driving force is provided to the slide 5 away from the tip of the forceps arm 1, and after the slide 5 is driven to push the two pipetting tips 8 away from the plug 11, under the reaction force of the spring 3, the slide 5 drives the pushing head 2 to resume the station, facilitating the reinstallation of new pipetting tips. At the same time, the movement of the driving slider 5 is facilitated by the pressing plate 71.
The working principle is as follows: when the suction head box is used, the standard suction head 8 is firstly placed into the general suction head box, the standard suction head 8 is installed and taken, then high-temperature sterilization is carried out, the suction head box is opened, the plugs 11 corresponding to the two forceps arms 1 can be directly inserted into the standard suction head 8 in the suction head box, and the suction head is quickly installed. The explant inoculation or sterile transfer can now be performed by pressing the forceps arms 1 to pinch the two standard tips 8. Accomplish an explant inoculation or aseptic transfer, press clamp plate 71, clamp plate 71 promotes two slides 5 and moves towards the direction of standard suction head, finally under the thrust effect of connecting rod 4 and cutting somebody's hair 2, unloads two standard suction heads 8 from plug 11 simultaneously, and is simple swift, improvement inoculation efficiency that can be very big.
Repeating the steps, and carrying out new explant inoculation or sterile transfer operation.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (7)
1. A tweezers for explant aseptic inoculation is characterized in that: including tweezers, the head of two tweezers arms of tweezers is provided with the plug, peg graft on the plug and have disposable plug, it is used for pushing away disposable plug to slide on the plug the cutting head of plug, be provided with on the tweezers arm and be used for the drive the gliding drive assembly of cutting head.
2. The forceps for the aseptic inoculation of explants according to claim 1, wherein: the disposable plug is a liquid transfer suction head, and the plug is arranged into a cylindrical structure matched with the liquid transfer suction head.
3. The forceps for the aseptic inoculation of explants according to claim 2, wherein: the driving assembly comprises sliding seats which are arranged on the two forceps arms in a sliding mode respectively, the sliding seats are connected with the corresponding pushing heads in a transition mode through connecting rods, a first connecting plate and a second connecting plate are arranged on the inner sides of the sliding seats respectively, and the first connecting plate is connected with the second connecting plate in a sliding mode.
4. Forceps for the sterile inoculation of explants according to claim 3, characterized in that: the first connecting plate is provided with a connecting raised head, the second connecting plate is provided with a guide groove matched with the connecting raised head, the guide groove is arc-shaped, and the circle center of the guide groove is overlapped with the rotating connection point of the two forceps arms.
5. The forceps for the aseptic inoculation of explants according to claim 4, wherein: one side of plug is provided with dodges the mouth, the centre of a circle department of pushing away the head just is located the inside of plug be provided with push away head transitional coupling's connecting portion, the push away the head pass through connecting portion with connecting rod transitional coupling.
6. Forceps for the sterile inoculation of explants according to claim 5, characterized in that: an elastic piece used for blocking the sliding seat from moving towards the head part of the forceps arm is arranged between one sliding seat and the forceps arm.
7. The forceps for the aseptic inoculation of explants according to claim 6, wherein: and one forceps arm is provided with a fixed seat, a corresponding sliding seat on the forceps arm is provided with a driving rod penetrating through the fixed seat, the suspended end of the driving rod is provided with a pressing plate, and the elastic part is a spring which is sleeved outside the driving rod and is positioned between the fixed arm and the pressing plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221809191.2U CN217487208U (en) | 2022-07-14 | 2022-07-14 | Tweezers for explant sterile inoculation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221809191.2U CN217487208U (en) | 2022-07-14 | 2022-07-14 | Tweezers for explant sterile inoculation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217487208U true CN217487208U (en) | 2022-09-27 |
Family
ID=83342019
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221809191.2U Active CN217487208U (en) | 2022-07-14 | 2022-07-14 | Tweezers for explant sterile inoculation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217487208U (en) |
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2022
- 2022-07-14 CN CN202221809191.2U patent/CN217487208U/en active Active
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