CN218058970U - Microalgae continuous subculture device - Google Patents

Abstract

The utility model discloses a microalgae continuous subculture device, which comprises a culture solution bottle, a microalgae seed bottle, a waste solution bottle, a stirring device, a peristaltic pump and connecting pipelines; the utility model discloses utilize the miniature instrument equipment little algae continuous subculture device that laboratory is commonly used, adopt the automatic method of trading the liquid in the airtight container, solve little algae and cultivate passage manual operation loaded down with trivial details, and have the puzzlement of pollution possibility. Just the utility model discloses well used instrument is the common small-size instrument equipment in laboratory, low price and easy operation, and the experimenter can assemble the debugging by oneself.

Description

Microalgae continuous subculture device

Technical Field

The utility model belongs to the technical field of bioengineering, a little algae continuous passage culture device is related to particularly.

Background

Microalgae such as Haematococcus pluvialis and Nannochloropsis oculata are rich in protein, unsaturated fatty acid, polysaccharides with various structures, vitamins and other active substances, and can be widely applied to the fields of nutritional health food, biomedicine, cosmetics, fine chemical industry and other products. At present, the demand for natural resources is increasing due to the development of human society and the demand for population increase, and the development and utilization of microalgae provide a new important solution for solving the human demand for various energy sources. The laboratory basic research can provide support and basis for the high-value development and utilization of microalgae.

When basic research is carried out in a laboratory, in order to ensure the quality of microalgae, the microalgae in the laboratory needs to be subcultured periodically. Currently most laboratories perform microalgae passaging, usually in a clean bench by laboratory personnel, but this approach has a number of disadvantages, 1): the manual operation process is relatively cumbersome. 2) The handling process is prone to contamination if careless. 3) The method needs to be carried out in an ultra-clean workbench, and has high dependence on instruments and equipment. Therefore, there is a need for an apparatus that can be used in common laboratories to replace such manual passage operations, so as to improve the efficiency of experimental operations and reduce the risk of contamination.

At present, the method and the device for culturing and subculturing the microalgae related to the utility model patent are mainly applied to large-scale production, for example, the patent CN103756886B utility model discloses a method and a device for continuously culturing the microalgae at high density, which are suitable for large-scale continuous culture of the microalgae and are not suitable for being carried out in a laboratory, the patent CN111394253A introduces a method for continuously culturing the nannochloropsis oculata at high density, the method refers to preparing a culture medium in a photoreactor, then inoculating the nannochloropsis oculata, culturing for 3-5 days, then discharging half of the culture solution every 3 days, supplementing an isovolumetric fresh culture medium and then continuing to culture, thereby realizing the continuous high-density culture of the nannochloropsis oculata, but the method does not refer to a specific operation mode, and can also mainly carry out microalgae inoculation and liquid change operation by manpower, so that the potential pollution risk exists.

SUMMERY OF THE UTILITY MODEL

The utility model aims to overcome the defects of the prior art and provide a microalgae continuous subculture device.

The utility model discloses a realize through following technical scheme:

a continuous subculture device for microalgae comprises a culture solution bottle, an algae seed bottle, a waste solution bottle, a stirring device, a peristaltic pump and connecting pipelines;

the culture solution bottle is connected to the liquid inlet end of the algae seed bottle through a liquid inlet pipe, the liquid inlet pipe is installed on a first peristaltic pump and provides power through the first peristaltic pump, the liquid outlet end of the algae seed bottle is connected with a liquid outlet pipe, the liquid outlet pipe is installed on a second peristaltic pump and provides power through the second peristaltic pump, the tail end of the liquid outlet pipe is connected with a three-way valve, and the three-way valve is respectively connected with an algae seed collecting pipe and a waste liquid collecting pipe; the algae seed bottle is arranged on the stirring device.

In the technical scheme, the microalgae continuous subculture device is integrally arranged on the culture platform.

In the technical scheme, the culture solution bottle is provided with a sterile filter.

In the technical scheme, the number of the culture solution bottles can be more than 1, and the culture solution bottles are used for containing algae culture media.

In the above technical solution, the first peristaltic pump and the second peristaltic pump are used for conveying the culture solution, and may preferably be single-channel peristaltic pumps or multi-channel peristaltic pumps.

In the technical scheme, the number of the algae strain bottles can be 1, and the algae strain bottles are used for culturing algae strains.

In the technical scheme, the stirring device is used for uniformly stirring the algae liquid in the algae seed culture bottle, has 6-8 channels, and is suitable for simultaneously culturing a plurality of bottles of algae seeds.

Compared with the prior art, the utility model has the advantages of:

the utility model discloses utilize the miniature instrument equipment microalgae continuous passage culture device that the laboratory is commonly used, adopt the automatic method of trading liquid in the airtight container, solve microalgae and cultivate passage manual operation loaded down with trivial details, and have the puzzlement of pollution possibility. Just the utility model discloses well used instrument is the common small-size instrument and equipment in laboratory, low price and easy operation, and the experimenter can assemble the debugging by oneself.

Drawings

Fig. 1 is a schematic view of the connection structure of the present invention.

FIG. 2 is a diagram showing the morphological comparison of different microalgae (Dunaliella salina, phaeodactylum tricornutum and Nannochloropsis) in different subculture methods in the examples.

Wherein: the device comprises a culture solution bottle 1, a first peristaltic pump 2, a stirring device 3, a waste solution bottle 4, a sterile filter 5, a liquid inlet pipe 6, an algae seed bottle 7, a liquid outlet pipe 8, a three-way valve 9, an algae seed collecting pipe 10, a waste solution collecting pipe 11, a culture platform 12 and a second peristaltic pump 13.

For a person skilled in the art, other relevant figures can be obtained from the above figures without inventive effort.

Detailed Description

The technical solution of the present invention will be further described with reference to the following specific embodiments.

Example 1

A microalgae continuous subculture device comprises a culture solution bottle 1, an algae seed bottle 7, a waste solution bottle 5, a stirring device 3, a peristaltic pump and connecting pipelines;

the culture solution bottle 1 is connected to the liquid inlet end of the algae seed bottle 7 through a liquid inlet pipe 6, the liquid inlet pipe 6 is installed on a first peristaltic pump 2 and provides power through the first peristaltic pump 2, the liquid outlet end of the algae seed bottle 7 is connected with a liquid outlet pipe 8, the liquid outlet pipe 8 is installed on a second peristaltic pump 13 and provides power through the second peristaltic pump 13, the tail end of the liquid outlet pipe 6 is connected with a three-way valve 9, and the three-way valve 9 is respectively connected with an algae seed collecting pipe 10 and a waste liquid collecting pipe 11; the algae seed bottle 7 is arranged on the stirring device 3.

In this embodiment, the whole microalgae continuous subculture apparatus is disposed on the culture platform 12.

In this embodiment, the culture solution bottle 1 is provided with a sterile filter 5.

In this embodiment, the number of the culture solution bottles 1 is 1, and the culture solution bottles are used for containing the algae culture medium.

In the present embodiment, the first peristaltic pump 2 and the second peristaltic pump 13 are used for culture solution delivery, and may preferably be single-channel peristaltic pumps or multi-channel peristaltic pumps.

In this embodiment, the number of the algal species bottles 7 is 1, and the bottles are used for culturing algal species.

In this embodiment, the stirring device 3 is used for uniformly stirring the algae liquid in the algae seed culture bottle, has 6-8 channels, and is suitable for simultaneously culturing a plurality of bottles of algae seeds.

The main working method of the device is as follows:

1) The culture bottle, culture medium bottle, waste liquid bottle, bottle cap, liquid-separating valve and liquid-feeding and-discharging conduit are sterilized, then assembled in the ultra-clean bench, in which the culture medium bottle is added with proper quantity of sterile algae culture medium, the culture bottle is inoculated with proper quantity of algae and added with stirring rotor, and the waste liquid bottle is added with proper quantity of disinfectant, and the bottle caps are respectively screwed, and after the sealing treatment, the culture bottle is connected with transfusion conduit.

2) Moving the whole device into a culture room, adjusting the room temperature and illumination, starting the stirring device, and then culturing and observing.

3) After the algae grows to a certain density, liquid changing and passage operation are needed, and the liquid inlet conduit and the liquid outlet conduit are respectively connected to the peristaltic pumps. Firstly, adjusting the speed of a peristaltic pump liquid according to actual requirements, starting a peristaltic pump 2, closing the peristaltic pump 2 after the algae liquid in a culture bottle is pumped out by a certain volume, then starting a peristaltic pump 1, and pumping a culture medium into an algae seed culture bottle to supplement nutrients required by microalgae; if algae seeds need to be taken, the algae seed culture solution is collected from the algae seed collecting pipe by controlling the switch of the three-way valve.

4) If a plurality of microalgae are cultured simultaneously, a plurality of culture bottles can be configured, and a multi-channel peristaltic pump is used for passage operation.

Example 2

1 sterility test

The microalgae culture medium was sampled before bottling, sampled through a culture flask, and finally sampled through a waste liquid bottle, and then cultured for one week without any contamination. The experiment was repeated 5 times without any contamination of the algal solution.

2 time to use test

The algae species are subjected to passage by conventional operation, and the whole process from experimental preparation to operation completion requires at least 30min. The process comprises ultra-clean bench ultraviolet sterilization for 15min, and liquid change operation for about 10-15min. If the liquid changing and subculturing treatment is carried out on a plurality of microalgae at the same time, longer time is needed. And by utilizing the continuous subculture device, except for the time consumed for the former assembly, the subsequent liquid changing culture operation only needs to start a peristaltic pump switch and adjust related parameters, so that the subculture operation can be completed within 3-5min without operating in a super clean bench, the subculture seed preserving efficiency is effectively improved, and the dependence on larger equipment is reduced.

Some microalgae culture solution bottles can adopt 2-5L of volume under normal state, and the dosage is maintained for 1-2 months, and the method can save the frequent operation of adding liquid in a super clean bench.

3 Effect on algal species quality

The algae seeds preserved by the conventional subculture and the algae seeds cultured by the continuous subculture for four weeks are respectively taken for morphological observation, as shown in figure 2, the algae seeds cultured by the two methods have no obvious difference in the growth speed and the growth state, which indicates that the growth speed and the growth state of the algae seeds cultured by using the continuous subculture device are not influenced.

The above description of the invention is given by way of example only, and it is to be understood that any simple modifications, adaptations or equivalent replacements which can be carried out by a person skilled in the art without expending any inventive effort, fall within the scope of protection of the invention, without departing from the core of the invention.

Claims (7)

1. A microalgae continuous subculture device is characterized by comprising a culture solution bottle, a microalgae seed bottle, a waste solution bottle, a stirring device, a peristaltic pump and connecting pipelines;

the culture solution bottle is connected to the liquid inlet end of the algae seed bottle through a liquid inlet pipe, the liquid inlet pipe is installed on a first peristaltic pump and provides power through the first peristaltic pump, the liquid outlet end of the algae seed bottle is connected with a liquid outlet pipe, the liquid outlet pipe is installed on a second peristaltic pump and provides power through the second peristaltic pump, the tail end of the liquid outlet pipe is connected with a three-way valve, and the three-way valve is respectively connected with an algae seed collecting pipe and a waste liquid collecting pipe; the algae seed bottle is arranged on the stirring device.

2. The continuous subculture apparatus for microalgae according to claim 1, wherein: the microalgae continuous subculture device is integrally arranged on the culture platform.

3. The continuous subculture apparatus for microalgae according to claim 1, wherein: and the culture solution bottle is provided with a sterile filter.

4. The continuous subculture apparatus for microalgae according to claim 1, wherein: the number of the culture solution bottles is more than 1.

5. The continuous subculture apparatus for microalgae according to claim 1, wherein: the first peristaltic pump and the second peristaltic pump are single-channel peristaltic pumps or multi-channel peristaltic pumps.

6. The continuous subculture apparatus for microalgae according to claim 1, wherein: the number of the algae seed bottles is more than 1.

7. The continuous subculture apparatus for microalgae according to claim 1, wherein: the stirring device has 6-8 channels.

Images