CN217438144U - Nucleic acid divides liquid and nucleic acid extraction element - Google Patents

Abstract

The utility model discloses a nucleic acid separating and nucleic acid extracting device, which comprises a portal frame, a separating module and a nucleic acid extracting module, wherein the separating module and the nucleic acid extracting module are suspended on the portal frame; the liquid separation module drives the suction pipe to move in the Z-axis direction, so that a sample in the pipe is sucked and sampled, and the sample is added into the detection test tube; the nucleic acid extraction module can drive the magnetic rod control assembly and the rod sleeve control assembly to move in the Z axial direction, and the magnetic rod with the rod sleeve is inserted into the detection test tube to extract nucleic acid; the utility model has the advantages of compact structure, reasonable components, integrated movement of the liquid separating module and the nucleic acid extracting module by using one portal frame, intensive functions, simplified spatial layout and reduced cost; the belt is used for transmission, and the guide rod and the guide rail are combined and oriented, so that smooth movement and accurate positioning are guaranteed; the automatic rifle head, accurate spy liquid, the setting of prevention of liquid leakage of moving liquid module, prevent cross contamination.

Description

Nucleic acid divides liquid and nucleic acid extraction element

Technical Field

The utility model relates to a medical treatment check out test set especially relates to a nucleic acid divides liquid and nucleic acid extraction element.

Background

Nucleic acids (including DNA and RNA) are commonly used as raw materials for various analyses and experiments in medicine, pharmaceutical research, and clinical diagnostics. At present, sufficiently large amounts of nucleic acids are readily available by various automated in vitro nucleic acid amplification techniques, such as the well-known Polymerase Chain Reaction (PCR) technique, but often require extraction or purification of the nucleic acids prior to amplification.

The nucleic acid extraction scheme mainly comprises the following steps: silicon dielectric column method, suction method, magnetic rod method. The magnetic rod method is based on a solid adsorption matrix, such as magnetic microparticles, which is capable of specifically and reversibly binding and releasing nucleic acids that have been released in the sample to be extracted. For example, when a magnetic field is applied, the magnetic particles with the nucleic acids bound thereto are gathered to a predetermined region to separate the magnetic particles from the sample, and moved to another liquid environment, and after the magnetic field is removed, the magnetic particles with the nucleic acids bound thereto can be collected for the purpose of extracting the nucleic acids.

Before extracting, need snatch the sample test tube, sweep the sign indicating number, reliably visit liquid and sample to intraductal liquid and shift. After the transfer, nucleic acid extraction is needed, and the operation of loading and unloading the rod sleeve and uniformly mixing the whole plate can be used in the extraction operation. Generally involving 3 large motion modules for grasping, pipetting, and extracting. In the prior art, the motion modules are arranged independently, so that the defects of large equipment volume and high cost are caused.

Therefore, how to design a device for separating and extracting nucleic acid with compact structure and relatively low cost is a technical problem to be solved urgently in the industry.

SUMMERY OF THE UTILITY MODEL

In order to solve the above-mentioned defect that exists among the prior art, the utility model provides a nucleic acid divides liquid and nucleic acid extraction element.

The utility model discloses a nucleic acid divides liquid and nucleic acid extraction element, it includes portal frame, divides liquid module, nucleic acid extraction module, wherein the portal frame is located the crossbeam of nucleic acid detector and can be along the crossbeam to do X axial displacement, divide liquid module and nucleic acid extraction module to hang on the portal frame, can be along the portal frame to do Y axial displacement; the liquid separation module is suspended on the portal frame, a suction pipe can be replaced at the bottom of the portal frame, the suction pipe can be driven to move in the Z-axis direction, a sample in the sample pipe is sucked and sampled, and the sample is added into the detection test tube; the nucleic acid extraction module is hung on the portal frame, can drive the magnetic rod control assembly and the rod sleeve control assembly to move in the Z axial direction, can drive the magnetic rod to be inserted into or separated from the rod sleeve, and inserts the magnetic rod with the rod sleeve into the detection test tube for nucleic acid extraction.

The portal frame comprises a Y-axis motor, a frame-shaped Y-axis supporting plate, a belt, a Y-axis guide rod and a Y-axis guide rail which are parallel to each other; the Y-axis support plate spans the front cross beam and the rear cross beam of the nucleic acid detector to provide support force for the device and comprises a first longitudinal beam and a second longitudinal beam, the Y-axis guide rod is arranged below the first longitudinal beam, and the Y-axis guide rail is arranged below the second longitudinal beam; the Y-axis motor is arranged at the far end of the Y-axis supporting plate to drive the belt to move; the belt is arranged along the first longitudinal beam and is connected with the nucleic acid extraction module, and the Y-axis motor drives the belt to move when rotating and drives the nucleic acid extraction module to move along the Y-axis guide rod in the Y-axis direction; the liquid separating module is connected with the nucleic acid extracting module and is linked with the nucleic acid extracting module to move along the Y-axis guide rail in the Y-axis direction.

The output shaft of the Y-axis motor is provided with a driving wheel, the driven wheel is arranged at the near end of the Y-axis supporting plate, the peripheries of the driving wheel and the driven wheel are sleeved with the belt, and the nucleic acid extraction module is connected with the belt through an extraction module mounting seat.

The nucleic acid extraction module comprises a vertical bracket, a magnetic rod control component and a rod sleeve control component, wherein the magnetic rod control component and the rod sleeve control component can move up and down along the bracket; the magnetic rod control assembly is arranged in the middle of the rod sleeve control assembly, can move up and down along with the rod sleeve control assembly and can drive the magnetic rod to be inserted into or pulled out of the rod sleeve; the rod sleeve control assembly can drive the rod sleeve positioned at the bottom end of the rod sleeve to move up and down; the magnetic rod control assembly and the rod sleeve control assembly move up and down along the first Z-axis guide rail and the first Z-axis guide rod; and a Y-axis linear bearing fixing seat is arranged on the side surface of the rear wall plate, and a linear bearing matched with the Y-axis guide rod is fixed inside the Y-axis linear bearing fixing seat.

The rod sleeve control assembly comprises a first motor, a first screw rod, a first driving plate, a first support and a rod sleeve mounting plate; the first motor is arranged on an extraction module mounting seat, and the extraction module mounting seat is arranged at the top end of the rear wall plate; the first screw rod is vertically arranged below the first motor and outputs the power of the first motor; a linear bearing and a nut are embedded in the first driving plate, the linear bearing is sleeved on the first Z-axis guide rod, the nut is meshed with the first screw rod, and the first driving plate moves up and down along the guide rod under the transmission of the first screw rod; the first support comprises a left wall plate and a right wall plate which are fixed on the left side and the right side of the first driving plate, and a space between the left wall plate and the right wall plate is used for accommodating the magnetic bar operating assembly; the rod sleeve mounting plate is mounted at the bottom ends of the left wall plate and the right wall plate, and the rod sleeve is suspended below the rod sleeve mounting plate.

The bottom of left side wallboard and right wallboard is connected the excellent cover installing frame that a level was placed, excellent cover installing frame is closed towards the magnetic rod and is formed towards the opening just ahead of structure, by three frames, and the medial surface of controlling two relative frames all is equipped with a horizontally recess, but two relative recesses level inserts excellent cover mounting panel.

The magnetic bar operating assembly comprises a second motor, a second screw rod, a second driving plate and a second support; the second motor is fixed on the lower surface of the transverse mounting plate, and one side of the transverse mounting plate is fixed on the top of the left wall plate; the second screw rod is vertically arranged above the second motor and outputs the power of the second motor; the second driving plate comprises a guide plate and a horizontal plate which are fixedly connected with each other, a nut meshed with the second screw rod is embedded in the horizontal plate, a linear bearing embedded on the first Z-axis guide rod is embedded in the guide plate, and the second driving plate moves up and down along the guide rod under the transmission of the second screw rod; the second support is hung below the horizontal plate, and the magnetic rod is fixed at the bottom end of the second support.

The second support comprises a back vertical plate, a side vertical plate and a horizontal connecting plate, the back vertical plate is positioned between the second motor and the first driving plate, the side vertical plate is positioned between the second motor and the right wall plate, and the upper ends of the back vertical plate and the side vertical plate are connected with the horizontal plate, and the lower ends of the back vertical plate and the side vertical plate are connected with the horizontal connecting plate; the back vertical plate, the side vertical plate and the horizontal connecting plate are connected into a whole, the horizontal connecting plate is located below the second motor, and the magnetic rod is fixed on the lower surface of the horizontal connecting plate.

The liquid separating module comprises a lifting module and a liquid absorbing module fixed on the lifting module; the lifting module comprises a vertical supporting plate, a third motor, a third screw rod, a second Z-axis guide rail and a third driving plate, the vertical supporting plate is connected with the support through a transverse connecting plate, the second Z-axis guide rail is vertically arranged along the vertical supporting plate, the third motor is arranged at the top end of the vertical supporting plate, the third screw rod is vertically arranged below the third motor, and power of the third motor is output; a nut is embedded in the third driving plate, the nut is meshed with a third screw rod, and the third driving plate moves up and down along a second Z-axis guide rail under the transmission of the third screw rod; the imbibition module is including fourth motor, vacuum pump, transmission, suction head that from top to bottom connects gradually to and control circuit board, the third drive plate is connected through a transverse fixing plate to the fourth motor, the suction head is located the transmission lower extreme and can pegs graft with the suction pipe, the fourth motor passes through transmission drive suction head and stretches out and draws back from top to bottom, vacuum pump connection suction head makes the suction head produce suction, control circuit board fixes on the vacuum pump lateral wall, is used for controlling the vacuum pump.

The middle part of the vertical supporting plate is provided with a hinged shaft, the hinged shaft is hinged with a connecting rod, the bottom end of the connecting rod is fixedly provided with a waste liquid blocking piece, a tension spring is arranged between the connecting rod and the supporting plate and used for pulling the waste liquid blocking piece away from the suction head, and a waste liquid cam block is arranged on the side wall of the transmission device at a position higher than the hinged shaft; when the vacuum pump moves the waste liquid cam block upwards to abut against the upper part of the connecting rod, the waste liquid blocking piece is retracted and blocked below the suction head.

The utility model provides a technical scheme's beneficial effect is:

the utility model has the advantages of compact structure, reasonable components, integrated movement of the liquid separating module and the nucleic acid extracting module by using one portal frame, intensive functions, simplified spatial layout and reduced cost; the belt is used for transmission, and the guide rod and the guide rail are combined and oriented, so that smooth movement and accurate positioning are guaranteed; the automatic rifle head, accurate spy liquid, the setting of prevention of liquid leakage of moving liquid module, prevent cross contamination.

Drawings

The invention is explained in more detail below with reference to exemplary embodiments and the accompanying drawings, in which:

FIG. 1 is an exploded perspective view of the preferred embodiment shown exploded from the left side;

FIG. 2 is an exploded perspective view of the preferred embodiment shown exploded from the right;

FIG. 3 is a right side perspective view of the preferred embodiment;

FIG. 4 is a left side perspective view of the preferred embodiment;

FIG. 5 is a front view of the preferred embodiment;

FIG. 6 is a rear view of the preferred embodiment;

FIG. 7 is a left side perspective view of a nucleic acid extraction module according to the preferred embodiment;

FIG. 8 is a right side perspective view of a nucleic acid extraction module according to one embodiment;

FIG. 9 is a left side perspective view of a dispensing module according to the preferred embodiment;

FIG. 10 is a right side perspective view of a dispensing module according to the preferred embodiment;

FIG. 11 is a top view of the preferred embodiment;

FIG. 12 is a right side view of the preferred embodiment;

figure 13 is a bottom view of the preferred embodiment.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention will be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

The to-be-solved technical problem of the utility model is: this patent is with nucleic acid extraction sample test tube move liquid and nucleic acid extraction design into integrative motion, solve present snatch, move liquid, draw the too complicated problem of 3 big motion modules.

The utility model discloses a nucleic acid divides liquid and nucleic acid extraction element, attached figure 1, fig. 2, fig. 3, fig. 4, fig. 5, fig. 6, fig. 11, fig. 12, fig. 13 show respectively that preferred embodiment left side direction explosion decomposes three-dimensional view, right side direction three-dimensional view, left side direction three-dimensional view, front view, back view, top view, right side view, bottom view. As can be seen from the figure, the device comprises a portal frame 100, a liquid separation module 200 and a nucleic acid extraction module 300, wherein the portal frame is arranged on a beam of the nucleic acid detector and can move along the beam in the X axial direction, and the liquid separation module and the nucleic acid extraction module are hung on the portal frame and can move along the portal frame in the Y axial direction; the liquid separation module is suspended on the portal frame, a suction pipe can be replaced at the bottom of the portal frame, the suction pipe can be driven to move in the Z-axis direction, a sample in the sample pipe is sucked and sampled, and the sample is added into the detection test tube; the nucleic acid extraction module is suspended on the portal frame, can drive the magnetic rod control assembly and the rod sleeve control assembly to move in the Z axial direction, can drive the magnetic rod 1 to be inserted into or separated from the rod sleeve 2, and inserts the magnetic rod with the rod sleeve into the detection test tube to extract nucleic acid.

During the detection, add the sample in the sample pipe into the detection test tube by dividing the liquid module one by one now, the detection test tube is arranged in the frame is placed to the detection test tube of a rectangle. In order to improve the detection efficiency, generally, nucleic acid extraction is uniformly carried out after the detection test tube placing frame is filled. The number of the detection test tubes of the magnetic rod and the rod sleeve is the same as that of the detection test tubes in the frame, and the magnetic rod and the rod sleeve are arranged into the same rectangle, so that the nucleic acid extraction can be simultaneously carried out on a plurality of detection test tubes.

Referring to the preferred embodiment shown in fig. 1 and 2, the gantry 100 includes a Y-axis motor 3, a frame-shaped Y-axis support plate, a belt 5, a Y-axis guide bar 6 and a Y-axis guide rail 7 which are parallel to each other; wherein the Y-axis support plate spans on the front and the rear cross beams of the nucleic acid detector to provide support force for the device and comprises a first longitudinal beam 4a and a second longitudinal beam 4b, the Y-axis guide rod is arranged below the first longitudinal beam, and the Y-axis guide rail is arranged below the second longitudinal beam; the Y-axis motor is arranged at the far end of the Y-axis supporting plate to drive the belt to move; the belt is arranged along the first longitudinal beam and is connected with the nucleic acid extraction module, and the Y-axis motor drives the belt to move when rotating and drives the nucleic acid extraction module to move along the Y-axis guide rod in the Y-axis direction; the liquid separating module is connected with the nucleic acid extracting module and is linked with the nucleic acid extracting module to move along the Y-axis guide rail in the Y-axis direction.

Since the liquid separation and the nucleic acid extraction cannot be performed simultaneously (liquid separation one by one and then unified nucleic acid extraction), the liquid separation module and the nucleic acid extraction module can be fixed and linked together. Because only one portal frame, one guide mechanism, one motor and a transmission mechanism are needed, the size can be reduced, and the cost is reduced.

In a preferred embodiment, the output shaft of the Y-axis motor 3 is provided with a driving wheel 8, a driven wheel 9 is arranged at the proximal end of the Y-axis support plate, the driving wheel and the driven wheel are sleeved with the belt 5, and the nucleic acid extraction module is connected with the belt through an extraction module mounting seat 17. In the preferred embodiment, the belt 5 is a toothed timing belt, and the driving pulley 8 and the driven pulley 9 are toothed timing pulleys. And the synchronous belt is adopted for transmission, so that the displacement is accurate and the resistance is small. Referring to fig. 8 in conjunction with fig. 2 or 3, the extraction module mounting base 17 includes an upper clamp plate and a lower clamp plate between which the belt is clamped, the lower clamp plate adopting a rack structure for engaging and driving the belt.

Referring to FIGS. 7 and 8, which illustrate a left side perspective view and a right side perspective view of a preferred embodiment nucleic acid extraction module 300, the nucleic acid extraction module includes a vertical support, a magnetic rod manipulating assembly and a rod sleeve manipulating assembly that can move up and down along the support; the magnetic rod control assembly is arranged in the middle of the rod sleeve control assembly, can move up and down along with the rod sleeve control assembly, and can drive the magnetic rod 1 to be inserted into or separated from the rod sleeve 2; the rod sleeve control assembly can drive the rod sleeve positioned at the bottom end of the rod sleeve to move up and down; the bracket comprises a vertical rear wall plate 10, a first Z-axis guide rail 11 and a first Z-axis guide rod 12 which are vertically fixed at the left side and the right side of the rear wall plate, and the magnetic rod control assembly and the rod sleeve control assembly move up and down along the first Z-axis guide rail and the first Z-axis guide rod; the side of back wallboard is equipped with a Y axle linear bearing fixing base 20, this Y axle linear bearing fixing base inside fixed with the linear bearing of Y axle guide rod 6 complex.

Because the magnetic rod control assembly and the rod sleeve control assembly are respectively provided with the motors and can independently run up and down, the two sets of motors can control the magnetic rod and the rod sleeve to run in the opposite direction or back to the back simultaneously, and meanwhile, the magnetic rod control assembly is arranged in the middle of the rod sleeve control assembly and can move up and down along with the rod sleeve control assembly, so that the time for loading/unloading the rod sleeve and inserting and separating the rod sleeve into and from a test tube is greatly shortened, and the working efficiency is high. When the magnetic rod operating structure is used, the magnetic rod operating structure is hung on a supporting beam of the nucleic acid detector, the supporting beam drives the magnetic rod operating structure to move in the X-axis direction and the Y-axis direction, and the magnetic rod operating structure drives the magnetic rod and the rod sleeve to move on the Z-axis direction, so that the magnetic rod and the rod sleeve can perform various nucleic acid extraction operations in various detection test tubes.

In connection with the preferred embodiment shown in fig. 7 and 8, the rod and sleeve operating assembly comprises a first motor 13, a first lead screw 14, a first drive plate 15, a first support and a rod and sleeve mounting plate 16; the first motor is mounted on an extraction module mounting seat 17, which is mounted at the top end of the rear wall plate 10; the first screw rod is vertically arranged below the first motor and outputs the power of the first motor; a linear bearing and a nut are embedded in the first driving plate, the linear bearing is sleeved on the first Z-axis guide rod 12, the nut is meshed with the first screw rod, and the first driving plate moves up and down along the guide rod under the transmission of the first screw rod; the first support comprises a left wall plate 18 and a right wall plate 19 which are fixed at the left side and the right side of the first driving plate, and a space between the left wall plate and the right wall plate is used for accommodating the magnetic bar operating assembly; the rod sleeve mounting plate is arranged at the bottom ends of the left wall plate and the right wall plate, and the rod sleeve 2 is suspended below the rod sleeve mounting plate.

In the preferred embodiment, the bottom ends of the left wall plate 18 and the right wall plate 19 are connected to a horizontally disposed rod sleeve mounting frame 27, which is open towards the front of the magnetic rod control structure and is formed by three side frames, and the inner side surfaces of the left and right opposite side frames are both provided with a horizontal groove, and the two opposite grooves can be horizontally inserted into the rod sleeve mounting plate 16. The rod sleeve is disposable, and needs to be replaced after one-time detection. The rod sleeve 2 and the rod sleeve mounting plate 9 are integrally formed, and the rod sleeve mounting plate can be conveniently and quickly replaced through the groove. Fig. 8 shows a bottom view, where the sleeve 2 and the sleeve mounting plate 9 are also clearly visible.

Referring to fig. 7 and 8, the magnetic bar operating assembly comprises a second motor 21, a second lead screw 22, a second driving plate and a second support; the second motor is fixed on the lower surface of the transverse mounting plate 23, and one side of the transverse mounting plate is fixed on the top of the left wall plate 18; the second screw rod is vertically arranged above the second motor and outputs the power of the second motor; the second driving plate comprises a guide plate 24a and a horizontal plate 24b which are fixedly connected with each other, a nut 25 meshed with a second screw rod is embedded in the horizontal plate, a linear bearing embedded on the first Z-axis guide rod 12 is embedded in the guide plate, and the second driving plate moves up and down along the guide rod under the transmission of the second screw rod; the second support is hung below the horizontal plate, and the magnetic rod 1 is fixed at the bottom end of the second support.

When the supporting beam of the nucleic acid detector drives the magnetic rod control structure to move in the X-axis direction and the Y-axis direction and go to a specified detection test tube (or leave the detection test tube), the magnetic rod control assembly can drive the magnetic rod to be inserted into (or separated from) the rod sleeve. Therefore, the work efficiency can be improved, and the detection time can be shortened.

With reference to fig. 7 and 8, the second support includes a back vertical plate, a side vertical plate 26b and a horizontal connecting plate 26c, the back vertical plate is located between the second motor 21 and the first driving plate 15, the side vertical plate is located between the second motor and the right wall plate 19, and upper ends of the back vertical plate and the side vertical plate are connected with the horizontal plate 24b, and lower ends of the back vertical plate and the side vertical plate are connected with the horizontal connecting plate; the back vertical plate, the side vertical plate and the horizontal connecting plate are connected into a whole, the horizontal connecting plate is located below the second motor, and the magnetic rod 1 is fixed on the lower surface of the horizontal connecting plate. The magnetic rod control structure is arranged in the middle of the rod sleeve control assembly and shares a set of guide elements with the rod sleeve control assembly. The purposes of compact structure and reduced volume are achieved.

Since the magnetic rod can be used repeatedly, for the convenience of assembly, the magnetic rod is fixed on the magnetic rod fixing plate, and then the magnetic rod fixing plate is fixed on the bottom surface of the horizontal connecting plate 26 c. In order to improve the detection efficiency, samples in a plurality of test tubes need to be detected. Therefore, the magnetic rods 1 are provided with a plurality of magnetic rods which are horizontally arranged into a matrix; the number of the rod sleeves 2 is equal to that of the magnetic rods, and the rod sleeves are horizontally arranged into a matrix; the position of the magnetic rod is up-down corresponding to the position of the rod sleeve, so that the magnetic rod can be conveniently inserted into or pulled out of the rod sleeve. In use the tubes are also arranged in the same matrix to facilitate insertion or removal of the sleeve 2.

Referring to fig. 9 and 10, which illustrate a left side perspective view and a right side perspective view of a liquid separating module of a preferred embodiment, the liquid separating module 200 includes a lifting module and a liquid absorbing module fixed on the lifting module; the lifting module comprises a vertical support plate 30, a third motor 31, a third screw rod 32, a second Z-axis guide rail 33 and a third drive plate 34, the vertical support plate is connected with the support through a transverse connecting plate 35, the second Z-axis guide rail is vertically arranged along the vertical support plate, the third motor is arranged at the top end of the vertical support plate, the third screw rod is vertically arranged below the third motor, and power of the third motor is output; a nut is embedded in the third driving plate, the nut is meshed with a third screw rod, and the third driving plate moves up and down along a second Z-axis guide rail under the transmission of the third screw rod; the liquid suction module comprises a fourth motor 37, a vacuum pump 39, a transmission device 38, a suction head 41 and a control circuit board 40 which are sequentially connected from top to bottom; the fourth motor is connected with a third drive plate through a transverse fixing plate 36, the suction head is positioned at the lower end of the transmission device and can be spliced with the suction pipe, the fourth motor drives the suction head to stretch up and down through the transmission device, the vacuum pump is connected with the suction head to enable the suction head to generate suction, and the control circuit board is fixed on the outer side wall of the vacuum pump and used for controlling the vacuum pump.

Before moving the liquid, divide the liquid module to move new straw board top, third motor 31 rotates, drive third lead screw 32, third drive plate 34 and imbibition module are down, lead screw in the transmission 38 down makes suction head 41 hit and gets the straw, suction head and straw peg graft together, then lifting module does and mentions suction head and straw, this device inserts the straw and surveys liquid with the sample pipe, control circuit board 40 is surveyed through the negative pressure, accurate discernment liquid level position, then control vacuum pump 39 works, the straw absorbs the sample, then this device takes the straw to detect the test tube top, pour into the sample (also called the liquid feeding). After the liquid adding is finished, the straws are brought to the upper part of the waste straw collecting box, the screw rod in the transmission device 38 moves downwards to the bottom, and the straws can automatically fall into the waste straw collecting box. The liquid separating module is an existing product and purchased from the market, and the internal structure of the liquid separating module is not repeated.

In order to prevent the sample inside the straw from leaking and dripping during the moving process in the straw, a movable waste liquid baffle plate needs to be arranged below the straw. Referring to fig. 9 and 10, in the preferred embodiment, a hinge shaft is provided at the middle of the vertical support plate 30, a connecting rod 42 is hinged to the hinge shaft, a waste liquid blocking plate 43 is fixed at the bottom end of the connecting rod, a tension spring 44 is provided between the connecting rod and the support plate, the tension spring pulls the waste liquid blocking plate away from the suction head 41, and a waste liquid cam block is provided on the side wall of the transmission device 38 at a position higher than the hinge shaft; when the vacuum pump moves the waste liquid cam block upwards to abut against the upper part of the connecting rod, the waste liquid blocking piece is retracted and blocked below the suction head.

When the liquid separating module drives the suction head 41 to move downwards, the waste liquid cam block is separated from the connecting rod 42, the waste liquid blocking piece is pulled open under the action of the tension spring 44, and the suction head 41 and the suction pipe can move freely without being influenced. When the liquid separating module drives the suction head 41 to move upwards to the end part, the waste liquid cam block is propped against the upper end of the connecting rod 42, the connecting rod is pressed vertically, and the waste liquid blocking piece 43 is blocked below the suction head or the suction pipe.

The foregoing examples are illustrative only and are not intended to be limiting. Any equivalent modifications or variations without departing from the spirit and scope of the present application should be included in the claims of the present application.

Claims (10)

1. A nucleic acid divides liquid and nucleic acid extraction element which characterized in that: comprises a portal frame (100), a liquid separating module (200) and a nucleic acid extracting module (300), wherein

The portal frame is erected on a beam of the nucleic acid detector and can move along the beam in the X axial direction, and the liquid separation module and the nucleic acid extraction module are hung on the portal frame and can move along the portal frame in the Y axial direction;

the liquid separation module is suspended on the portal frame, a suction pipe can be replaced at the bottom of the portal frame, the suction pipe can be driven to move in the Z-axis direction, a sample in the sample pipe is sucked and sampled, and the sample is added into the detection test tube;

the nucleic acid extraction module is hung on the portal frame, can drive the magnetic rod control assembly and the rod sleeve control assembly to move in the Z axial direction, can drive the magnetic rod (1) to be inserted into or separated from the rod sleeve (2), and inserts the magnetic rod with the rod sleeve into the detection test tube to extract nucleic acid.

2. The nucleic acid separating and nucleic acid extracting apparatus according to claim 1, wherein: the portal frame (100) comprises a Y-axis motor (3), a frame-shaped Y-axis supporting plate, a belt (5), a Y-axis guide rod (6) and a Y-axis guide rail (7) which are parallel to each other; wherein

The Y-axis support plate spans the front cross beam and the rear cross beam of the nucleic acid detector and provides support force for the device, and comprises a first longitudinal beam (4 a) and a second longitudinal beam (4 b), the Y-axis guide rod is arranged below the first longitudinal beam, and the Y-axis guide rail is arranged below the second longitudinal beam;

the Y-axis motor is arranged at the far end of the Y-axis supporting plate to drive the belt to move;

the belt is arranged along the first longitudinal beam and is connected with the nucleic acid extraction module, and the Y-axis motor drives the belt to move when rotating and drives the nucleic acid extraction module to move along the Y-axis guide rod in the Y-axis direction;

the liquid separating module is connected with the nucleic acid extracting module and is linked with the nucleic acid extracting module to move along the Y-axis guide rail in the Y-axis direction.

3. The nucleic acid separating and nucleic acid extracting apparatus according to claim 2, wherein: a driving wheel (8) is installed on an output shaft of the Y-axis motor (3), a driven wheel (9) is installed at the near end of the Y-axis supporting plate, the belt (5) is sleeved on the peripheries of the driving wheel and the driven wheel, and the nucleic acid extraction module is connected with the belt through an extraction module installing seat (17).

4. The nucleic acid separating and nucleic acid extracting apparatus according to claim 3, wherein: the nucleic acid extraction module (300) comprises a vertical bracket, a magnetic rod manipulation assembly and a rod sleeve manipulation assembly, wherein the magnetic rod manipulation assembly and the rod sleeve manipulation assembly can move up and down along the bracket; the magnetic rod control component is arranged in the middle of the rod sleeve control component, can move up and down along with the rod sleeve control component, and can drive the magnetic rod (1) to be inserted into or pulled out of the rod sleeve (2); the rod sleeve control assembly can drive the rod sleeve positioned at the bottom end of the rod sleeve to move up and down; the bracket comprises a vertical rear wall plate (10), a first Z-axis guide rail (11) and a first Z-axis guide rod (12), wherein the first Z-axis guide rail and the first Z-axis guide rod are vertically fixed at the left side and the right side of the rear wall plate, and the magnetic rod control assembly and the rod sleeve control assembly move up and down along the first Z-axis guide rail and the first Z-axis guide rod; the side of back wallboard is equipped with one Y axle linear bearing fixing base (20), this Y axle linear bearing fixing base inside fixed with Y axle guide bar (6) complex linear bearing.

5. The nucleic acid separating and nucleic acid extracting apparatus according to claim 4, wherein: the rod sleeve control assembly comprises a first motor (13), a first screw rod (14), a first driving plate (15), a first support and a rod sleeve mounting plate (16);

the first motor is arranged on an extraction module mounting seat (17), and the extraction module mounting seat is arranged at the top end of the rear wall plate (10);

the first screw rod is vertically arranged below the first motor and outputs the power of the first motor;

a linear bearing and a nut are embedded in the first driving plate, the linear bearing is sleeved on the first Z-axis guide rod (12), the nut is meshed with the first screw rod, and the first driving plate moves up and down along the guide rod under the transmission of the first screw rod;

the first support comprises a left wall plate (18) and a right wall plate (19) which are fixed at the left side and the right side of the first driving plate, and a space between the left wall plate and the right wall plate is used for accommodating the magnetic bar operating assembly;

the bar sleeve mounting plate is mounted at the bottom ends of the left wall plate and the right wall plate, and the bar sleeve (2) is suspended below the bar sleeve mounting plate.

6. The nucleic acid separating and nucleic acid extracting apparatus according to claim 5, wherein: the bottom of left side wallboard (18) and right wallboard (19) is connected stick cover installing frame (27) that a level was placed, stick cover installing frame is towards the opening of bar magnet control structure dead ahead, is enclosed by three frames and is formed, and the medial surface of controlling two relative frames all is equipped with a horizontally recess, but two relative recesses level inserts stick cover mounting panel (16).

7. The nucleic acid separating and extracting apparatus according to claim 5, wherein: the magnetic bar operating assembly comprises a second motor (21), a second screw rod (22), a second driving plate and a second support;

the second motor is fixed on the lower surface of the transverse mounting plate (23), and one side of the transverse mounting plate is fixed on the top of the left wall plate (18);

the second screw rod is vertically arranged above the second motor and outputs the power of the second motor;

the second driving plate comprises a guide plate (24 a) and a horizontal plate (24 b) which are fixedly connected with each other, a nut (25) meshed with the second screw rod is embedded in the horizontal plate, a linear bearing embedded on the first Z-axis guide rod (12) is embedded in the guide plate, and the second driving plate moves up and down along the guide rod under the transmission of the second screw rod;

the second support is hung below the horizontal plate, and the magnetic rod (1) is fixed at the bottom end of the second support.

8. The nucleic acid separating and nucleic acid extracting apparatus according to claim 7, wherein: the second support comprises a back vertical plate, a side vertical plate (26 b) and a horizontal connecting plate (26 c), the back vertical plate is located between the second motor (21) and the first driving plate (15), the side vertical plate is located between the second motor and the right wall plate (19), and the upper ends of the back vertical plate and the side vertical plate are connected with the horizontal plate (24 b), and the lower ends of the back vertical plate and the side vertical plate are connected with the horizontal connecting plate; the back vertical plate, the side vertical plate and the horizontal connecting plate are connected into a whole, the horizontal connecting plate is located below the second motor, and the magnetic rod (1) is fixed on the lower surface of the horizontal connecting plate.

9. The nucleic acid separating and nucleic acid extracting apparatus according to claim 4, wherein: the liquid separating module (200) comprises a lifting module and a liquid absorbing module fixed on the lifting module; wherein

The lifting module comprises a vertical supporting plate (30), a third motor (31), a third screw rod (32), a second Z-axis guide rail (33) and a third driving plate (34), the vertical supporting plate is connected with the bracket through a transverse connecting plate (35), the second Z-axis guide rail is vertically installed along the vertical supporting plate, the third motor is installed at the top end of the vertical supporting plate, and the third screw rod is vertically installed below the third motor and outputs power of the third motor; a nut is embedded in the third driving plate, the nut is meshed with a third screw rod, and the third driving plate moves up and down along a second Z-axis guide rail under the transmission of the third screw rod;

imbibition module is including fourth motor (37), vacuum pump (39), transmission (38), suction head (41) that from top to bottom connect gradually to and control circuit board (40), the third drive plate is connected through a horizontal fixed plate (36) to the fourth motor, the suction head is located the transmission lower extreme and can pegs graft with the suction pipe, the fourth motor passes through transmission drive suction head and stretches out and draws back from top to bottom, vacuum pump connection suction head makes the suction head produce suction, control circuit board fixes on the vacuum pump lateral wall, is used for controlling the vacuum pump.

10. The nucleic acid separating and extracting apparatus according to claim 9, wherein: a hinged shaft is arranged in the middle of the vertical supporting plate (30), a connecting rod (42) is hinged to the hinged shaft, a waste liquid blocking piece (43) is fixed to the bottom end of the connecting rod, a tension spring (44) is arranged between the connecting rod and the supporting plate, the waste liquid blocking piece is pulled away from the suction head (41) by the tension spring, and a waste liquid cam block is arranged on the side wall of the transmission device (38) at a position higher than the hinged shaft; when the vacuum pump moves the waste liquid cam block upwards to abut against the upper part of the connecting rod, the waste liquid blocking piece is retracted and blocked below the suction head.

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