CN112904032A - Small-sized full-automatic chemiluminescence analysis system - Google Patents
Small-sized full-automatic chemiluminescence analysis system Download PDFInfo
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- CN112904032A CN112904032A CN202110196657.XA CN202110196657A CN112904032A CN 112904032 A CN112904032 A CN 112904032A CN 202110196657 A CN202110196657 A CN 202110196657A CN 112904032 A CN112904032 A CN 112904032A
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- 238000004458 analytical method Methods 0.000 title claims abstract description 45
- 238000002156 mixing Methods 0.000 claims abstract description 90
- 238000011534 incubation Methods 0.000 claims abstract description 72
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 59
- 238000004140 cleaning Methods 0.000 claims abstract description 44
- 238000001514 detection method Methods 0.000 claims abstract description 37
- 238000007885 magnetic separation Methods 0.000 claims abstract description 30
- 230000003287 optical effect Effects 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 239000002699 waste material Substances 0.000 claims abstract description 13
- 238000002347 injection Methods 0.000 claims abstract description 7
- 239000007924 injection Substances 0.000 claims abstract description 7
- 230000005540 biological transmission Effects 0.000 claims description 26
- 239000000758 substrate Substances 0.000 claims description 11
- 238000010438 heat treatment Methods 0.000 claims description 9
- 238000000926 separation method Methods 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 27
- 230000006870 function Effects 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 107
- 239000003085 diluting agent Substances 0.000 description 4
- 238000002203 pretreatment Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000004590 computer program Methods 0.000 description 3
- 239000012470 diluted sample Substances 0.000 description 3
- 238000003113 dilution method Methods 0.000 description 3
- 238000003018 immunoassay Methods 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000036046 immunoreaction Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/026—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having blocks or racks of reaction cells or cuvettes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
- G01N2035/00356—Holding samples at elevated temperature (incubation)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
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Abstract
The invention discloses a small-sized full-automatic chemiluminescence analysis system which comprises a rack, a control part and an analysis part, wherein the rack is provided with a bottom plate, and the control part is fixed on the rack; the analysis part comprises a main control board, and a reaction cup assembly, a three-dimensional hand grip assembly, a reagent sample assembly, an incubation assembly, a magnetic separation cleaning assembly, an optical detection assembly, a blending assembly, a sample injection needle assembly and a waste liquid needle assembly which are arranged on the bottom board and are respectively connected with the main control board; the main control board is connected with the control part; the control part controls each component of the analysis part through the main control board. The small-sized full-automatic chemiluminescence analysis system provided by the invention realizes and supports various reaction methodologies by integrating all components for chemiluminescence analysis on the rack, and has the advantages of high automation degree, small volume, simple structure, low cost and complete functions.
Description
Technical Field
The invention relates to the technical field of small-sized full-automatic chemiluminescence analysis, in particular to a small-sized full-automatic chemiluminescence analysis system.
Background
In the field of in vitro diagnostics, chemiluminescence immunoassay (CLIA) is an analytical technique for quantitatively detecting various antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins, drugs, and the like by combining a chemiluminescence assay method with high sensitivity and a high-specificity immunoreaction method. The full-automatic chemiluminescence immunoassay analyzer comprises a sample module, a reagent module, a reaction module, a detection module and other functional modules. The detection process of the analyzer generally includes: firstly, respectively placing a sample and a reagent into a sample module and a reagent module, then adding the sample and the reagent into a reaction cup, respectively carrying out incubation, cleaning and other systems on the reaction cup, and finally, enabling the reaction cup to enter a detection disc to finish the determination. At present, the chemiluminescence immunoassay analyzer in the industry generally has the conditions of large volume, complex structure, high cost, high failure rate and excessive parts.
Disclosure of Invention
The invention provides a small-sized full-automatic chemiluminescence analysis system, and aims to solve the problems of a chemiluminescence analyzer in the prior art.
In order to achieve the purpose, the invention provides a small-sized full-automatic chemiluminescence analysis system, which comprises a rack, a control part and an analysis part, wherein the rack is provided with a bottom plate, and the control part is fixed on the rack; the analysis part comprises a main control board, and a reaction cup assembly, a three-dimensional hand grip assembly, a reagent sample assembly, an incubation assembly, a magnetic separation cleaning assembly, an optical detection assembly, a blending assembly, a sample injection needle assembly and a waste liquid needle assembly which are arranged on the bottom board and are respectively connected with the main control board; the main control board is connected with the control part; the control part controls each component of the analysis part through the main control board.
Preferably, the three-dimensional gripper assembly comprises a first rail arranged horizontally, a second rail arranged on the first rail, a mechanical arm arranged on the second rail, and a mechanical gripper arranged on the mechanical arm; the second track moves along the track direction of the first track; the mechanical arm moves along the track direction of the second track; the mechanical hand moves along the telescopic direction of the mechanical arm.
Preferably, the reaction cup assembly is arranged on the edge of the bottom plate on one side of the first rail close to the second rail; the reaction cup assembly comprises a reaction cup tray and a third rail; the reaction cup tray is arranged on the third rail so as to realize drawer-type pulling out of the reaction cup tray through the third rail.
Preferably, the reagent sample assembly is arranged on one side of the reaction cup assembly away from the first track; the reagent sample assembly comprises a bracket, a driving device, a transmission device and a tray; the transmission device comprises a transmission shaft, a fixed gear and a kit transmission gear; the driving device is arranged below the bottom plate, the bracket is fixed on the bottom plate, and the transmission shaft is arranged in the bracket; the transmission device is connected with the driving device; the driving device drives the transmission shaft to drive the fixed gear to rotate, and the rotation of the fixed gear drives the kit transmission gear to rotate; the tray is provided with a reagent box containing position corresponding to the reagent box transmission gear, and the reagent box is placed on the containing position; the tray is also provided with a sample jacket for holding sample tubes, and the sample tubes are placed in the sample jacket.
Preferably, the incubation assembly is arranged in the middle of the bottom plate; the incubation assembly comprises a heating device and an incubation tray; the heating device is arranged below the incubation disc and is used for heating and insulating the incubation disc; the incubation component is used for carrying out incubation reaction on the sample in the reaction cup.
Preferably, the magnetic separation cleaning assembly is arranged on a bottom plate on one side of the incubation assembly away from the reaction cup assembly; the magnetic separation cleaning assembly is used for carrying out four-stage cleaning and separation on the sample in the reaction cup after incubation and carrying out substrate filling on the sample in the reaction cup after cleaning and separation.
Preferably, the optical detection assembly is arranged on the bottom plate between the incubation assembly and the first track; the optical detection assembly is used for optically detecting the sample in the reaction cup.
Preferably, the blending component is arranged on the bottom plate on one side of the incubation component far away from the optical detection component, and comprises a blending driving motor, an eccentric blending column, a detection optocoupler and a blending bin; the blending driving motor is arranged on the lower side of the bottom plate, and the eccentric blending column is arranged on the upper side of the bottom plate corresponding to the blending driving motor; the blending driving motor is connected with the eccentric blending column to drive the eccentric blending column to work; the detection optical coupler is arranged on the side wall of the blending bin and connected with the eccentric blending column to realize optical coupler detection; the panel of the mixing bin is provided with a reaction cup hole corresponding to the eccentric mixing column, and the reaction cup hole is used for placing a reaction cup.
Preferably, the sample injection needle assembly is arranged on one side of the reagent sample assembly; the sample feeding needle assembly comprises a vertical arm, a horizontal arm and a sample feeding needle, wherein the vertical arm is fixedly arranged on the bottom plate, the horizontal arm is arranged at one end of the vertical arm far away from the bottom plate, and the horizontal arm rotates around the vertical arm; the sample adding needle is arranged at one end of the horizontal arm far away from the vertical arm and moves up and down along the direction vertical to the bottom plate.
Preferably, the waste liquid needle assembly is arranged at one side of the magnetic separation cleaning assembly and is used for sucking waste liquid in the reaction cup after the test.
The small-sized full-automatic chemiluminescence analysis system provided by the invention realizes and supports various reaction methodologies by integrating all components for chemiluminescence analysis on the rack, and has the advantages of high automation degree, small volume, simple structure, low cost and complete functions.
Drawings
FIG. 1 is a schematic perspective view of a small-scale fully-automatic chemiluminescence analysis system according to an embodiment of the invention;
FIG. 2 is a schematic top view of a small-sized fully-automatic chemiluminescence analysis system according to an embodiment of the invention;
fig. 3 is a schematic perspective view of a three-dimensional gripper assembly according to an embodiment of the present invention;
FIG. 4 is a schematic perspective view of a reaction cup assembly according to an embodiment of the present invention;
FIG. 5 is a schematic cross-sectional view taken along line A-A of FIG. 4;
FIG. 6 is a schematic cross-sectional view taken along line B-B of FIG. 4;
FIG. 7 is a schematic perspective view of a reaction cup assembly according to an embodiment of the present invention;
FIG. 8 is a schematic side view of a reaction cup assembly according to an embodiment of the present invention;
FIG. 9 is a schematic flow chart of a one-step method A according to an embodiment of the present invention;
FIG. 10 is a schematic flow chart of a one-step method B provided in the second embodiment of the present invention;
FIG. 11 is a schematic flow chart of a two-step process provided in the third embodiment of the present invention;
FIG. 12 is a schematic flow chart of a pre-dilution method according to a fourth embodiment of the present invention;
fig. 13 is a schematic flow chart of a pretreatment method according to a fifth embodiment of the present invention.
In the figure, 10, a frame; 11. a base plate; 21. a reaction cup assembly; 211. a reaction cup tray; 212. a third track; 2111. a reaction cup access hole; 22. a three-dimensional gripper assembly; 221. a first track; 222. a second track; 223. a mechanical arm; 224. a mechanical gripper; 2211. a first driving section; 2221. a second driving section; 2231. a third driving section; 23. a reagent sample assembly; 231. a support; 232. a drive device; 2331. a drive shaft; 2332. fixing a gear; 2333. a kit transmission gear; 234. a tray; 2341. a kit containing position; 2342. a sample jacket; 24. an incubation assembly; 25. a magnetic separation cleaning assembly; 26. an optical detection assembly; 27. a blending component; 271. uniformly mixing a driving motor; 272. an eccentric blending column; 273. detecting the optocoupler; 274. a blending bin; 2741. a side wall; 2742. a panel; 28. a sample injection needle assembly; 281. a vertical arm; 282. a horizontal arm; 283. a sample adding needle; 29. a waste liquid needle assembly; 100. a reaction cup; 101. a kit; 102. a sample tube; 110. a reaction cup hole.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1 and 2 in combination, an embodiment of the present invention provides a small-sized full-automatic chemiluminescence analysis system, which includes a rack 10, a control unit including a computer and a computer program running on the computer, and an analysis unit, specifically, the computer includes a processor and a memory, the memory stores the computer program for performing full-automatic chemiluminescence analysis, and the processor executes the computer program to perform full-automatic chemiluminescence analysis steps. The frame 10 is provided with a bottom plate 11, and the control part is fixed on the frame 10; of course, the control unit may be connected to the analysis unit outside the rack 10 via a network or a cable. The analysis part comprises a main control board, and a reaction cup assembly 21, a three-dimensional hand grip assembly 22, a reagent sample assembly 23, an incubation assembly 24, a magnetic separation cleaning assembly 25, an optical detection assembly 26, a blending assembly 27, a sample injection needle assembly 28 and a waste liquid needle assembly 29 which are arranged on the bottom board 11 and are respectively connected with the main control board; the main control board is connected with the control part; the control part controls each component of the analysis part through the main control board.
Referring to fig. 1, fig. 2 and fig. 3, in an embodiment of the present invention, the three-dimensional hand grip assembly 22 includes a first rail 221 horizontally disposed, a second rail 222 disposed on the first rail 221, a robot arm 223 disposed on the second rail 222, and a robot hand 224 disposed on the robot arm 223; the second rail 222 moves in a rail direction of the first rail 221, i.e., an X-axis direction; the robot arm 223 moves in the orbit direction of the second orbit 222, i.e., the Y-axis direction; the mechanical hand 224 moves in the telescopic direction of the robot arm 223, i.e., the Z-axis direction. Specifically, the first rail 221 further includes a first driving part 2211 for driving the second rail 222 to move, the second rail 222 further includes a second driving part 2221 for driving the robot arm 223 to move, and the robot arm 223 further includes a third driving part 2231 for driving the robot hand 224 to move. The mechanical hand grip 224 is used for gripping the reaction cup 100, and the mechanical hand grip 224 can move along the three-dimensional directions of the X axis, the Y axis and the Z axis through the structure of the three-dimensional hand grip assembly 22, so that the reaction cup 100 can move among the reaction cup assembly 21, the incubation assembly 24, the magnetic separation cleaning assembly 25, the optical detection assembly 26 and the blending assembly 27.
The reaction cup assembly 21 is arranged on the edge of the bottom plate 11 on one side of the first rail 221 close to the second rail 222, and the reaction cup assembly 21 is used for supplying a disposable reaction container; the reagent sample assembly 23 is arranged on one side of the reaction cup assembly 21 away from the first rail 221, and the reagent sample assembly 23 is used for storing reagents required by the reaction and samples to be detected; the incubation component 24 is arranged in the middle of the bottom plate 11, and the incubation component 24 is used for performing incubation reaction on a sample in the reaction cup; the magnetic separation cleaning assembly 25 is arranged on the bottom plate 11 on one side of the incubation assembly 24 away from the reaction cup assembly 21, the magnetic separation cleaning assembly 25 is used for performing four-stage cleaning and separation on a sample in the reaction cup after incubation and performing substrate filling on the sample in the reaction cup after cleaning and separation, and the structure of the magnetic separation cleaning assembly 25 is similar to that of a general magnetic separation cleaning device, and is not described in detail herein; the optical detection assembly 26 is disposed on the bottom plate 11 between the incubation assembly 24 and the first track 221, the optical detection assembly 26 is used for optically detecting a sample in the cuvette, and the optical detection assembly 26 is similar in structure to a general optical detection device, which is not described herein again; the blending component 27 is arranged on the bottom plate 11 on one side of the incubation component 24 away from the optical detection component 26, and the blending component 27 is used for blending the sample and the reagent in the reaction cup; the sample application needle assembly 28 is disposed at one side of the reagent sample assembly 23, and the sample application needle assembly 28 is used for adding a sample and a corresponding reaction reagent; the waste liquid needle assembly 29 is arranged at one side of the magnetic separation cleaning assembly 25, and the waste liquid needle assembly 29 is used for absorbing waste liquid in the reaction cup after the test.
Specifically, the reaction cup assembly 21 includes a reaction cup tray 211 and a third rail 212; the reaction cup tray 211 is mounted on the third rail 212 to draw out the reaction cup tray 211 in a drawer type through the third rail 212, so that a new reaction cup can be conveniently taken and placed. The reaction cup tray 211 is provided with a plurality of reaction cup access holes 2111 for facilitating fixing and storing reaction cups.
Referring to fig. 1, 2, 4, 5 and 6, the reagent sample assembly 23 includes a holder 231, a driving device 232, a transmission device and a tray 234; the transmission includes a drive shaft 2331, a fixed gear 2332, and a kit drive gear 2333; the driving device 232 is disposed below the bottom plate 11, the bracket 231 is fixed on the bottom plate 11, and the transmission shaft 2331 is disposed in the bracket 231; the transmission device is connected with the driving device 232; the driving device 232 drives the transmission shaft 2331 to rotate the fixed gear 2332, and the rotation of the fixed gear 2332 drives the reagent kit transmission gear 2333 to rotate; the tray 234 is provided with a reagent kit containing position 2341 corresponding to the reagent kit transmission gear 2333, and the reagent kit 101 is placed on the reagent kit containing position 2341; the tray 234 is also provided with a sample jacket 2342 for holding the sample tube 102, the sample tube 102 being placed in the sample jacket 2342.
The incubation assembly 24 comprises a heating device and an incubation tray; the heating device is arranged below the incubation disc and is used for heating and insulating the incubation disc; the reaction cups are placed on an incubation tray and the incubation assembly 24 is used to perform an incubation reaction on the samples in the reaction cups.
Referring to fig. 1, fig. 2, fig. 7 and fig. 8, the blending assembly 27 includes a blending driving motor 271, an eccentric blending column 272, a detecting optical coupler 273 and a blending bin 274; the blending driving motor 271 is arranged at the lower side of the bottom plate 11, and the eccentric blending column 272 is arranged at the upper side of the bottom plate 11 corresponding to the blending driving motor 271; the blending driving motor 271 is connected with the eccentric blending column 272 to drive the eccentric blending column 272 to work; the detection optocoupler 273 is arranged on the side wall 2741 of the blending bin 274 and connected with the eccentric blending column 272 to realize optocoupler detection; the panel 2742 of the blending bin 274 is provided with a reaction cup hole 110 corresponding to the eccentric blending column 272, and the reaction cup hole 110 is used for placing the reaction cup 100. The blending component 27 adopts an eccentric blending principle; the reaction cup 100 is placed in the reaction cup hole 110, and the eccentric blending column 272 is driven by the blending driving motor 271 to drive the reaction cup 100 to perform eccentric motion, so as to achieve the purpose of blending.
Referring again to fig. 1 and 2, the needle loading assembly 28 is disposed on one side of the reagent sample assembly 23; the needle assembly 28 comprises a vertical arm 281, a horizontal arm 282 and a needle 283, wherein the vertical arm 281 is fixedly arranged on the bottom plate 11, the horizontal arm 282 is arranged at one end of the vertical arm 281 far away from the bottom plate 11, and the horizontal arm 282 rotates around the vertical arm 281; the sample injection needle 283 is disposed at one end of the horizontal arm 282 away from the vertical arm 281 and moves up and down along a direction perpendicular to the bottom plate 11.
Specifically, in one embodiment of the invention, when the small-sized full-automatic chemiluminescence analysis system provided by the invention is used for analyzing components of a sample to be detected, necessary consumables including a new reaction cup, a substrate required by reaction and a cleaning solution are loaded; wherein the reaction cup is used as a test carrier, and the reaction combination of the reagent sample is completed in the reaction cup; the substrate is a reaction solution which needs to be added in each test process, and the cleaning solution is used for cleaning the reagent sample needle and cleaning the magnetic separation; when the test is carried out, a sample and a reagent are respectively placed on the tray of the reagent sample assembly, a new reaction cup is taken out of the reaction cup tray of the reaction cup assembly by using the mechanical gripper of the three-dimensional gripper assembly, and the new reaction cup is placed into the blending assembly. And then adding the sample and the reagent into the reaction cup according to a preset program, starting an incubation program and a cleaning program, and finally enabling the reaction cup to enter an optical detection assembly to complete the analysis of the sample components.
The parameters of detection flow, sequence, incubation time, sample adding amount and the like of different items related to chemiluminescence are different; the small-sized full-automatic chemiluminescence analysis system provided by the invention supports various chemiluminescence method detection methods; the method specifically comprises the following steps: one-step method A, one-step method B, two-step method, pre-dilution method, pretreatment method and the like.
The first embodiment is as follows: one-step Process A
Referring to fig. 9, a one-step method a according to an embodiment of the present invention includes:
step S101: the mechanical gripper grabs a new reaction cup from the reaction cup tray to the reaction cup hole of the blending assembly;
step S102: the sample adding needle sucks a sample of the sample tube in a tray of the reagent sample assembly, adds the sample into the reaction cup, and then cleans the sample adding needle;
step S103: moving the sample adding needle to a tray of the reagent sample assembly to suck a reagent, adding the reagent into the reaction cup, and then cleaning the sample adding needle;
step S104: after uniformly mixing the reaction cups by the uniformly mixing component, grabbing the reaction cups added with the samples and the reagents to an incubation plate by a mechanical gripper for incubation;
step S105: the mechanical gripper takes the reaction cup out of the incubation disc and sends the reaction cup to the magnetic separation cleaning assembly for four-stage magnetic separation cleaning, and a first substrate is added;
step S106: the mechanical gripper takes the reaction cup out of the magnetic separation cleaning assembly and sends the reaction cup to an incubation disc for incubation;
step S107: and the mechanical hand grip takes the reaction cup out of the incubation disc and sends the reaction cup to the optical detection assembly, and a second substrate is added to complete photometric detection.
Example two: one-step method B
Referring to fig. 10, a second one-step method B provided by the embodiment of the present invention includes:
step S201: the mechanical gripper grabs a new reaction cup from the reaction cup tray to the reaction cup hole of the blending assembly;
step S202: the sample adding needle sucks a sample of the sample tube in a tray of the reagent sample assembly, adds the sample into the reaction cup, and then cleans the sample adding needle;
step S203: moving the sample adding needle to a tray of the reagent sample assembly to suck a reagent, adding the reagent into the reaction cup, and then cleaning the sample adding needle;
step S204: after uniformly mixing the reaction cups by the uniformly mixing component, grabbing the reaction cups added with the samples and the reagents to an incubation plate by a mechanical gripper for incubation;
step S205: the mechanical gripper takes the reaction cup out of the incubation disc and sends the reaction cup to the blending component;
step S206: the sample adding needle is used for adding a sample to the reaction cup in the uniformly mixing component for the second time;
step S207: after uniformly mixing the reaction cups by the uniformly mixing component, grabbing the reaction cups to the incubation plate by the mechanical gripper for incubation;
step S208: the mechanical gripper takes the reaction cup out of the incubation disc and sends the reaction cup to the magnetic separation cleaning assembly for four-stage magnetic separation cleaning, and a first substrate is added;
step S209: the mechanical gripper takes the reaction cup out of the magnetic separation cleaning assembly and sends the reaction cup to an incubation disc for incubation;
step S210: and the mechanical hand grip takes the reaction cup out of the incubation disc and sends the reaction cup to the optical detection assembly, and a second substrate is added to complete photometric detection.
Example three: two-step process
Referring to fig. 11, a third two-step method according to the embodiment of the present invention includes:
step S301: the mechanical gripper grabs a new reaction cup from the reaction cup tray to the reaction cup hole of the blending assembly;
step S302: the sample adding needle sucks a sample of the sample tube in a tray of the reagent sample assembly, adds the sample into the reaction cup, and then cleans the sample adding needle;
step S303: moving the sample adding needle to a tray of the reagent sample assembly to suck a reagent, adding the reagent into the reaction cup, and then cleaning the sample adding needle;
step S304: after uniformly mixing the reaction cups by the uniformly mixing component, grabbing the reaction cups added with the samples and the reagents to an incubation plate by a mechanical gripper for incubation;
step S305: the mechanical gripper takes the reaction cup out of the incubation disc and sends the reaction cup to the magnetic separation cleaning assembly for four-stage magnetic separation cleaning;
step S306: the mechanical gripper takes the reaction cup out of the magnetic separation cleaning assembly and sends the reaction cup to the blending assembly;
step S307: the sample adding needle adds corresponding reagents to the reaction cup at the position of the uniform mixing component;
step S308: the mechanical gripper takes the reaction cup out of the blending component and sends the reaction cup to an incubation disc for incubation;
step S309: the mechanical gripper takes the reaction cup out of the incubation disc and sends the reaction cup to the magnetic separation cleaning assembly for four-stage magnetic separation cleaning, and a first substrate is added;
step S310: the mechanical gripper takes the reaction cup out of the magnetic separation cleaning assembly and sends the reaction cup to an incubation disc for incubation;
step S311: and the mechanical hand grip takes the reaction cup out of the incubation disc and sends the reaction cup to the optical detection assembly, and a second substrate is added to complete photometric detection.
Example four: method of predilution
Referring to fig. 12, a pre-dilution method according to a fourth embodiment of the present invention includes:
step S401: the mechanical gripper grabs a new reaction cup from the reaction cup tray to the reaction cup hole of the blending assembly;
step S402: the sample adding needle sucks a sample of the sample tube in a tray of the reagent sample assembly, adds the sample into the reaction cup, and then cleans the sample adding needle;
step S403: moving the sample adding needle to a tray of the reagent sample assembly to absorb the diluent, adding the diluent to the reaction cup, and then cleaning the sample adding needle;
step S404: the blending component is used for blending the reaction cups;
step S405: the sample adding needle sucks part of the uniformly mixed and diluted sample in the reaction cup at the uniformly mixing component and moves away;
step S406: the mechanical gripper takes out the reaction cup at the blending component and sends the reaction cup to a cup losing position to discard the reaction cup;
step S407: a mechanical gripper takes a new reaction cup from the reaction cup tray and sends the new reaction cup to the blending component, and a sample adding needle flows the diluted sample sucked from the previous reaction cup into the new reaction cup;
step S408: the test is continued according to the one-step method A, the one-step method B and the two-step method.
Example five: pretreatment method
Referring to fig. 13, a pretreatment method according to a fifth embodiment of the present invention includes:
step S501: the mechanical gripper grabs a new reaction cup from the reaction cup tray to the reaction cup hole of the blending assembly;
step S502: the sample adding needle sucks a sample of the sample tube in a tray of the reagent sample assembly, adds the sample into the reaction cup, and then cleans the sample adding needle;
step S503: moving the sample adding needle to a tray of the reagent sample assembly to absorb the diluent, adding the diluent to the reaction cup, and then cleaning the sample adding needle;
step S504: the blending component is used for blending the reaction cups;
step S505: the mechanical gripper takes the reaction cup out of the blending component and sends the reaction cup to an incubation disc for incubation;
step S506: after incubation is finished, the reaction cup is taken out from the incubation disc by the mechanical gripper and is sent to the blending assembly, and the sample adding needle absorbs part of the sample from the blending assembly reaction cup and moves away;
step S507: the mechanical gripper takes out the reaction cup at the blending component and sends the reaction cup to a cup losing position to discard the reaction cup;
step S508: a mechanical gripper takes a new reaction cup from the reaction cup tray and sends the new reaction cup to the blending component, and a sample adding needle flows the diluted sample sucked from the previous reaction cup into the new reaction cup;
step S509: the test is continued according to the one-step method A, the one-step method B and the two-step method.
Compared with the prior art, the small-sized full-automatic chemiluminescence analysis system provided by the invention realizes and supports various reaction methodologies by integrating all components for chemiluminescence analysis on the rack, and has the advantages of high automation degree, small volume, simple structure, low cost and complete functions.
While the foregoing is directed to embodiments of the present invention, it will be understood by those skilled in the art that various changes may be made without departing from the spirit and scope of the invention.
Claims (10)
1. A small-sized full-automatic chemiluminescence analysis system is characterized by comprising a rack, a control part and an analysis part, wherein the rack is provided with a bottom plate, and the control part is fixed on the rack; the analysis part comprises a main control board, and a reaction cup assembly, a three-dimensional hand grip assembly, a reagent sample assembly, an incubation assembly, a magnetic separation cleaning assembly, an optical detection assembly, a blending assembly, a sample injection needle assembly and a waste liquid needle assembly which are arranged on the bottom board and are respectively connected with the main control board; the main control board is connected with the control part; the control part controls each component of the analysis part through the main control board.
2. The small scale full-automatic chemiluminescence analysis system of claim 1, wherein the three-dimensional gripper assembly comprises a horizontally disposed first rail, a second rail disposed on the first rail, a robotic arm disposed on the second rail, and a robotic gripper disposed on the robotic arm; the second track moves along the track direction of the first track; the mechanical arm moves along the track direction of the second track; the mechanical hand moves along the telescopic direction of the mechanical arm.
3. The small scale full-automatic chemiluminescence analysis system according to claim 2, wherein the reaction cup assembly is arranged at the edge of the bottom plate at one side of the first rail close to the second rail; the reaction cup assembly comprises a reaction cup tray and a third rail; the reaction cup tray is arranged on the third rail so as to realize drawer-type pulling out of the reaction cup tray through the third rail.
4. The small scale full-automatic chemiluminescence analysis system according to claim 3, wherein the reagent sample assembly is disposed on a side of the reaction cup assembly away from the first track; the reagent sample assembly comprises a bracket, a driving device, a transmission device and a tray; the transmission device comprises a transmission shaft, a fixed gear and a kit transmission gear; the driving device is arranged below the bottom plate, the bracket is fixed on the bottom plate, and the transmission shaft is arranged in the bracket; the transmission device is connected with the driving device; the driving device drives the transmission shaft to drive the fixed gear to rotate, and the rotation of the fixed gear drives the kit transmission gear to rotate; the tray is provided with a reagent box containing position corresponding to the reagent box transmission gear, and the reagent box is placed on the containing position; the tray is also provided with a sample jacket for holding sample tubes, and the sample tubes are placed in the sample jacket.
5. The small scale full-automatic chemiluminescence analysis system according to claim 1, wherein the incubation assembly is disposed in the middle of the base plate; the incubation assembly comprises a heating device and an incubation tray; the heating device is arranged below the incubation disc and is used for heating and insulating the incubation disc; the incubation component is used for carrying out incubation reaction on the sample in the reaction cup.
6. The small scale full-automatic chemiluminescence analysis system according to claim 1, wherein the magnetic separation cleaning assembly is arranged on a bottom plate of the incubation assembly on a side away from the reaction cup assembly; the magnetic separation cleaning assembly is used for carrying out four-stage cleaning and separation on the sample in the reaction cup after incubation and carrying out substrate filling on the sample in the reaction cup after cleaning and separation.
7. The small scale full-automatic chemiluminescence analysis system according to claim 2, wherein the optical detection assembly is disposed on the base plate between the incubation assembly and the first track; the optical detection assembly is used for optically detecting the sample in the reaction cup.
8. The small-sized full-automatic chemiluminescence analysis system according to claim 1, wherein the blending component is arranged on the bottom plate on one side of the incubation component away from the optical detection component, and comprises a blending driving motor, an eccentric blending column, a detection optocoupler and a blending bin; the blending driving motor is arranged on the lower side of the bottom plate, and the eccentric blending column is arranged on the upper side of the bottom plate corresponding to the blending driving motor; the blending driving motor is connected with the eccentric blending column to drive the eccentric blending column to work; the detection optical coupler is arranged on the side wall of the blending bin and connected with the eccentric blending column to realize optical coupler detection; the panel of the mixing bin is provided with a reaction cup hole corresponding to the eccentric mixing column, and the reaction cup hole is used for placing a reaction cup.
9. The small scale full-automatic chemiluminescence analysis system according to claim 1, wherein the sample application needle assembly is disposed on one side of the reagent sample assembly; the sample feeding needle assembly comprises a vertical arm, a horizontal arm and a sample feeding needle, wherein the vertical arm is fixedly arranged on the bottom plate, the horizontal arm is arranged at one end of the vertical arm far away from the bottom plate, and the horizontal arm rotates around the vertical arm; the sample adding needle is arranged at one end of the horizontal arm far away from the vertical arm and moves up and down along the direction vertical to the bottom plate.
10. The small-sized full-automatic chemiluminescence analysis system according to claim 1, wherein the waste liquid needle assembly is provided on one side of the magnetic separation and washing assembly, and the waste liquid needle assembly is used for sucking up waste liquid in the reaction cup after the test.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110196657.XA CN112904032A (en) | 2021-02-22 | 2021-02-22 | Small-sized full-automatic chemiluminescence analysis system |
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| CN202110196657.XA CN112904032A (en) | 2021-02-22 | 2021-02-22 | Small-sized full-automatic chemiluminescence analysis system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114167071A (en) * | 2022-02-10 | 2022-03-11 | 广州科方生物技术股份有限公司 | Multifunctional sample analysis system |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108459168A (en) * | 2018-03-27 | 2018-08-28 | 深圳市科创志达技术有限公司 | A kind of Full-automatic chemiluminescence immunoassay analysis meter |
| CN110501513A (en) * | 2019-09-19 | 2019-11-26 | 南京迪格诺斯生物技术有限公司 | A kind of chemiluminescent analyzer |
| CN112051406A (en) * | 2020-07-20 | 2020-12-08 | 深圳雷杜生命科学股份有限公司 | Chemiluminescent immunoassay device |
| CN212119776U (en) * | 2019-12-28 | 2020-12-11 | 深圳市帝迈生物技术有限公司 | Blending device and immunoassay appearance |
-
2021
- 2021-02-22 CN CN202110196657.XA patent/CN112904032A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108459168A (en) * | 2018-03-27 | 2018-08-28 | 深圳市科创志达技术有限公司 | A kind of Full-automatic chemiluminescence immunoassay analysis meter |
| CN110501513A (en) * | 2019-09-19 | 2019-11-26 | 南京迪格诺斯生物技术有限公司 | A kind of chemiluminescent analyzer |
| CN212119776U (en) * | 2019-12-28 | 2020-12-11 | 深圳市帝迈生物技术有限公司 | Blending device and immunoassay appearance |
| CN112051406A (en) * | 2020-07-20 | 2020-12-08 | 深圳雷杜生命科学股份有限公司 | Chemiluminescent immunoassay device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114167071A (en) * | 2022-02-10 | 2022-03-11 | 广州科方生物技术股份有限公司 | Multifunctional sample analysis system |
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