CN216970386U

CN216970386U - Cell cryopreservation equipment for cell engineering

Info

Publication number: CN216970386U
Application number: CN202220528529.0U
Authority: CN
Inventors: 陈奥运
Original assignee: Hefei All Things Biotechnology Co ltd
Current assignee: Hefei All Things Biotechnology Co ltd
Priority date: 2022-03-11
Filing date: 2022-03-11
Publication date: 2022-07-15
Anticipated expiration: 2032-03-11

Abstract

The utility model relates to the technical field of cell cryopreservation equipment for cell engineering, in particular to cell cryopreservation equipment for cell engineering, which comprises a cryopreservation tank, wherein the side edge of the top end of the cryopreservation tank is communicated with a port, the port is provided with an end cover, and the top end surface of the end cover is communicated with a taking barrel; the top end face of the freezing tank is provided with a motor, a rotating disc in transmission connection with the motor is arranged in an inner cavity of the freezing tank, and a plurality of guide rail supporting cylinders are circumferentially arranged on the top end face of the rotating disc; split charging circular plates are inserted into the guide rail supporting cylinder, a limit top ring which is propped against the top end face of the guide rail supporting cylinder is connected above the split charging circular plates, a plurality of screw holes are circumferentially formed in the top end face of the split charging circular plates, and cell tubes are spirally connected in the screw holes; an electric push rod is arranged right below the upper edge port of the bottom end face of the freezing tank; the cell freezing equipment for cell engineering, which is designed by the utility model, has reasonable structural design, strong practicability and excellent market popularization value.

Description

Cell cryopreservation equipment for cell engineering

Technical Field

The utility model relates to the technical field of cell engineering auxiliary equipment, in particular to cell cryopreservation equipment for cell engineering.

Background

Cell engineering is the cross field of cell biology and genetics, and is a technology for changing or creating cytogenetics in a planned way by mainly utilizing the principles and methods of cell biology and combining the technical means of engineering according to the design of people in advance. Including mass culture and propagation of cells in vitro, or obtaining cell products, or using the cell bodies themselves. The main content comprises cell fusion, a cell bioreactor, chromosome transfer, organelle transplantation, gene transfer, cell and tissue culture.

In the process of cell engineering experiments, the cell metabolism needs to be reduced by freezing the cells, so that the cells can be stored for a long time, when the cell tubes are taken out from the freezing device at present, the freezing device is opened to expose all the inner test tubes, the cell tubes are taken out from the inside and then are closed, the heat inside and outside the device is opened, the internal temperature changes, the preservation effect of the rest of the cell tubes is influenced, the defects are improved, and the technical problem to be solved by technical personnel in the field is solved urgently.

SUMMERY OF THE UTILITY MODEL

Solves the technical problem

Aiming at the defects in the prior art, the utility model provides cell cryopreservation equipment for cell engineering, aiming at facilitating the taking of the cryopreserved cells and reducing the influence on the preservation effect of other cells in the taking process.

Technical scheme

In order to achieve the purpose, the utility model is realized by the following technical scheme:

a cell freezing device for cell engineering comprises a freezing tank, wherein a port is communicated with the side edge of the top end of the freezing tank, an end cover is arranged at the port, and a taking barrel is communicated with the top end face of the end cover; the top end face of the freezing storage tank is provided with a motor, a rotating disc in transmission connection with the motor is arranged in an inner cavity of the freezing storage tank, and a plurality of guide rail supporting cylinders are circumferentially arranged on the top end face of the rotating disc; split charging round plates are inserted into the guide rail supporting cylinders, limit top rings abutting against the top end faces of the guide rail supporting cylinders are connected above the split charging round plates, a plurality of screw holes are circumferentially formed in the top end faces of the split charging round plates, and cell tubes are spirally connected in the screw holes; an electric push rod is installed right below the port on the bottom end face of the freezing tank, and a top plate is fixed on a piston rod of the electric push rod.

Furthermore, a controller is installed on the top end face of the freezing tank, and a sealing cover is installed at the top end opening of the taking cylinder.

Furthermore, the motor is located at the center of the top end face of the cryopreservation tank, the rotating disc and the motor are coaxial up and down, the motor is a stepping motor and can control the rotating angle of the rotating disc at each time, and therefore the rotating disc can be driven to rotate at a specified angle through the motor.

Furthermore, a plurality of water conservancy diversion logical grooves are all circumferentially arranged on the side wall of the guide rail supporting cylinder, so that the gas in the guide rail supporting cylinder and the gas in the freezing storage tank can be conveniently exchanged and circulated.

Furthermore, a plurality of connecting rods are circumferentially fixed on the outer side of the top end face of the split charging circular plate, the top end of each connecting rod is connected to the bottom end face of the corresponding limiting top ring, the connecting rods and the split charging circular plates slide up and down in the guide rail supporting cylinders, and the outer diameter of each limiting top ring is larger than the inner diameter of each guide rail supporting cylinder.

Furthermore, an auxiliary circular plate is connected to the position right below the split charging circular plate through a support rod, and when the cell tube is spirally connected in the screw hole, the bottom end face of the cell tube is higher than the top end face of the auxiliary circular plate.

Furthermore, the inner diameter of the guide rail supporting cylinder is consistent with the inner diameter of the taking cylinder, the outer diameters of the top plate and the auxiliary circular plate are smaller than the inner diameter of the guide rail supporting cylinder, and the top plate and the taking cylinder are coaxial up and down.

Furthermore, the motor and the electric push rod are both electrically connected to a signal output end of the controller, and the working conditions of the motor and the electric push rod can be controlled through the controller.

Advantageous effects

Compared with the known public technology, the technical scheme provided by the utility model has the following beneficial effects:

according to the utility model, through the design of additionally arranging the freezing storage tank, the port, the end cover, the taking barrel, the motor, the rotating disc, the guide rail supporting barrel, the split charging circular plate, the limiting top ring, the screw hole, the cell tube, the electric push rod and the top plate, the split charging circular plate can be pushed into the taking barrel through the matching action of the electric push rod and the top plate when the cell tube is taken, then the required cell tube is taken out through taking, and the split charging circular plate is abutted to the lower port of the taking barrel in the process, so that the contact range of the freezing storage tank and the outside can be reduced to a certain extent, further cells in the freezing storage tank are not influenced, and the practicability is high.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It is obvious that the drawings in the following description are only some embodiments of the utility model, and that for a person skilled in the art, other drawings can be derived from them without inventive effort.

FIG. 1 is a schematic view of the internal structure of a cryopreservation tank of the present invention;

FIG. 2 is a schematic cross-sectional view of the present invention;

FIG. 3 is a schematic cross-sectional perspective view of the present invention;

FIG. 4 is an enlarged view of a portion of the present invention;

FIG. 5 is an exploded view of the present invention;

FIG. 6 is a schematic view of the overall structure of the present invention;

the reference numerals in the drawings denote: 1-freezing and storing the tank; 2-port; 3-end cover; 4-taking the cylinder; 5-an electric motor; 6-rotating the disc; 7-a guide rail support cylinder; 8-subpackaging the circular plates; 9-a limit top ring; 10-screw holes; 11-cell tube; 12-an electric push rod; 13-a top plate; 14-a controller; 15-sealing cover; 16-a flow guide through groove; 17-a connecting rod; 18-a support bar; 19-auxiliary round plate.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The present invention will be further described with reference to the following examples.

Examples

The cell freezing apparatus for cell engineering according to the present embodiment is described with reference to fig. 1 to 2: the freezing storage tank comprises a freezing storage tank 1, wherein a port 2 is communicated with the side edge of the top end of the freezing storage tank 1, an end cover 3 is arranged at the port 2, and a taking barrel 4 is communicated with the top end face of the end cover 3; the top end face of the freezing and storing tank 1 is provided with a motor 5, a rotating disc 6 which is connected with the motor 5 in a transmission way is arranged in the inner cavity of the freezing and storing tank 1, and a plurality of guide rail supporting cylinders 7 are circumferentially arranged on the top end face of the rotating disc 6.

Wherein, the top end surface of the freezing tank 1 is provided with a controller 14, and the top end opening 2 of the taking cylinder 4 is provided with a sealing cover 15; the motor 5 is located the center department that freezes the jar 1 top terminal surface of depositing, and the rotating disc 6 is coaxial from top to bottom with the motor 5, and the motor is step motor, can the controller angle of rotation at every turn to can drive rotating disc 6 through motor 5 and carry out the rotation of appointed angle.

Referring to FIGS. 3-4: split charging circular plates 8 are inserted into the guide rail supporting cylinders 7, limit top rings 9 which are abutted against the top end surfaces of the guide rail supporting cylinders 7 are connected above the split charging circular plates 8, a plurality of screw holes 10 are circumferentially formed in the top end surfaces of the split charging circular plates 8, and cell tubes 11 are spirally connected in the screw holes 10; an electric push rod 12 is arranged on the bottom end face of the freezing tank 1 along the position right below the port 2, and a top plate 13 is fixed on a piston rod of the electric push rod 12.

Referring to FIGS. 5-6: a plurality of flow guide through grooves 16 are circumferentially formed in the side wall of the guide rail supporting cylinder 7, so that the gas in the guide rail supporting cylinder 7 can be conveniently exchanged and circulated with the gas in the freezing tank 1; a plurality of connecting rods 17 are circumferentially fixed on the outer side of the top end face of the split charging circular plate 8, the top ends of the connecting rods 17 are connected to the bottom end face of the limiting top ring 9, the connecting rods 17 and the split charging circular plate 8 slide up and down in the guide rail supporting cylinder 7, and the outer diameter of the limiting top ring 9 is larger than the inner diameter of the guide rail supporting cylinder 7; an auxiliary circular plate 19 is connected to the lower part of the subpackaging circular plate 8 through a support rod 18, and when the cell tube 11 is screwed in the screw hole 10, the bottom end surface of the cell tube 11 is higher than the top end surface of the auxiliary circular plate 19; the inner diameter of the guide rail supporting cylinder 7 is consistent with the inner diameter of the taking cylinder 4, the outer diameters of the top plate 13 and the auxiliary circular plate 19 are both smaller than the inner diameter of the guide rail supporting cylinder 7, and the top plate 13 and the taking cylinder 4 are coaxial up and down; the motor 5 and the electric push rod 12 are both electrically connected to a signal output end of the controller 14, and the controller 14 can control the working conditions of the motor 5 and the electric push rod 12.

When in use, firstly, the inner cavity of the freezing and storing tank 1 is cleaned and disinfected, and then the controller 14 sets the rotating angle of the motor 5 every time, so that the motor 5 drives the rotating circular plate to rotate once, and the guide rail supporting cylinder 7 can be positioned under the taking cylinder 4 once; secondly, in the process of storing cells, the cells and the culture solution are firstly stored in a cell tube 11, then the cells of the same batch are sequentially screwed in screw holes 10 on the same subpackaging circular plate 8, and finally the subpackaging circular plate 8 is inserted into a guide rail supporting cylinder 7 along the port 2, because the outer diameter of the limiting top ring 9 is larger than the inner diameter of the guide rail supporting cylinder 7, the limiting top ring 9 can be abutted against the top end surface of the guide rail supporting cylinder 7, and the subpackaging circular plate 8 can be prevented from falling down along the guide rail supporting cylinder 7; when a dispensing circular plate 8 is mounted, the controller 14 controls the motor 5 to rotate once, so that the dispensing circular plates 8 can be sequentially placed in the guide rail supporting cylinder 7 along the port 2 (as shown in fig. 1).

At the in-process of cryopreserving the cell, earlier cover 3 in port 2 departments with end cover 15 lid again in the top port 2 department of a section of thick bamboo 4 of taking, through the liquid nitrogen cooling mechanism of cryopreserving jar 1 self, can carry out low temperature cryopreserving to the cell that cryopreserved jar 1 is inside (the liquid nitrogen cooling mechanism principle of here is prior art's liquid nitrogen container principle, does not do here too much to describe repeatedly). When a cell tube 11 on one of the sub-packaging circular plates 8 needs to be taken out, the controller 14 controls the motor 5 to rotate the guide rail supporting cylinder 7 on which the sub-packaging circular plate 8 is placed to the lower part of the taking cylinder 4 (in the process of storing the cell tube 11, the position of each cell tube 11 and the sub-packaging circular plate 8 needs to be recorded, so that the position of one of the sub-packaging circular plates 8 and the position of the cell tube 11 can be known quickly), then the electric push rod 12 is controlled to drive the top plate 13 to push upwards, at the moment, the auxiliary circular plate 19 is pushed by the top plate 13 to drive the sub-packaging circular plate 8 to move upwards in the guide rail supporting cylinder 7 until the sub-packaging circular plate 8 moves to the upper end of the taking cylinder 4, the sealing cover 15 is opened to take out the sub-packaging circular plate 8 by pulling the limit top ring 9 (as shown in figure 3), in the process, the sub-packaging circular plate 8 is abutted to the lower port 2 of the taking cylinder 4, so that the contact range of the frozen storage tank 1 with the outside can be reduced to a certain extent, thereby ensuring that other cells in the freezing storage tank 1 are not influenced. The cell freezing and storing equipment for cell engineering, which is designed by the utility model, has the advantages of reasonable structural design, strong practicability and excellent market popularization value.

The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the corresponding technical solutions.

Claims

1. The utility model provides a cell freezing equipment for cell engineering, includes freezing jar (1), its characterized in that: the side edge of the top end of the freezing tank (1) is communicated with a port (2), an end cover (3) is arranged at the port (2), and a taking barrel (4) is communicated with the top end face of the end cover (3); the top end face of the freezing tank (1) is provided with a motor (5), a rotating disc (6) in transmission connection with the motor (5) is arranged in an inner cavity of the freezing tank (1), and a plurality of guide rail supporting cylinders (7) are circumferentially arranged on the top end face of the rotating disc (6); split charging round plates (8) are inserted into the guide rail supporting cylinders (7), limit top rings (9) which are abutted against the top end faces of the guide rail supporting cylinders (7) are connected above the split charging round plates (8), a plurality of screw holes (10) are circumferentially formed in the top end faces of the split charging round plates (8), and cellular pipes (11) are spirally connected in the screw holes (10); electric putter (12) are installed along port (2) under on the bottom face of freezing jar (1), be fixed with roof (13) on electric putter (12)'s the piston rod.

2. The cell cryopreservation device for cell engineering according to claim 1, wherein a controller (14) is installed on the top end face of the cryopreservation tank (1), and a sealing cover (15) is installed at the top end opening (2) of the taking barrel (4).

3. The cell cryopreservation apparatus for cell engineering according to claim 1, wherein the motor (5) is located at the center of the top end face of the cryopreservation tank (1), and the rotating disk (6) and the motor (5) are coaxial up and down.

4. The cell cryopreservation equipment for cell engineering according to claim 1, wherein a plurality of flow guide through grooves (16) are circumferentially formed in the side wall of the guide rail supporting cylinder (7).

5. The cell cryopreservation apparatus for cell engineering according to claim 1, wherein a plurality of connecting rods (17) are circumferentially fixed to the outer side of the top end face of the split charging circular plate (8), the top ends of the connecting rods (17) are connected to the bottom end face of the top limiting ring (9), the connecting rods (17) and the split charging circular plate (8) both slide up and down in the guide rail supporting cylinder (7), and the outer diameter of the top limiting ring (9) is larger than the inner diameter of the guide rail supporting cylinder (7).

6. The cell cryopreservation apparatus for cell engineering according to claim 1, wherein an auxiliary circular plate (19) is connected to a position right below the split circular plate (8) through a support rod (18), and when the cell tube (11) is screwed into the screw hole (10), a bottom end surface of the cell tube (11) is higher than a top end surface of the auxiliary circular plate (19).

7. The cell cryopreservation device for cell engineering according to claim 1, wherein the inner diameter of the guide rail supporting cylinder (7) is consistent with the inner diameter of the taking cylinder (4), the outer diameters of the top plate (13) and the auxiliary circular plate (19) are both smaller than the inner diameter of the guide rail supporting cylinder (7), and the top plate (13) and the taking cylinder (4) are coaxial up and down.

8. The cell cryopreservation device for cell engineering according to claim 1, wherein the motor (5) and the electric push rod (12) are electrically connected to a signal output end of the controller (14).