CN216864151U - Filtering membrane type nucleic acid extraction kit - Google Patents
Filtering membrane type nucleic acid extraction kit Download PDFInfo
- Publication number
- CN216864151U CN216864151U CN202122726972.7U CN202122726972U CN216864151U CN 216864151 U CN216864151 U CN 216864151U CN 202122726972 U CN202122726972 U CN 202122726972U CN 216864151 U CN216864151 U CN 216864151U
- Authority
- CN
- China
- Prior art keywords
- cavity
- sample adding
- nucleic acid
- liquid storage
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a filtering membrane type nucleic acid extraction kit, which comprises a kit body (1), wherein a sample adding cavity (2) and a liquid storage cavity (3) are arranged on the kit body (1), and a connecting liquid path (4) is arranged between the sample adding cavity (2) and the liquid storage cavity (3); the sample adding cavity (2) is provided with an air hole (21) and a sample adding hole (22), and the liquid storage cavity (3) is provided with a liquid adding hole (31); and a filtering membrane (23) at the joint of the sample adding cavity (2) and the connecting liquid path (4). When the utility model is used for extracting nucleic acid of a sample, only a plurality of steps are needed to be completed in one kit, external pollution is not easy to be introduced, and the extraction efficiency can be greatly improved.
Description
Technical Field
The utility model relates to a filtering membrane type nucleic acid extraction kit, and belongs to the technical field of medical detection.
Background
With the popularization of technical means such as gene detection and the like, the limitation of the traditional nucleic acid extraction method is very obvious today when high throughput and automation are pursued. At present, the magnetic bead method for extracting nucleic acid can realize automatic extraction and large-batch operation, is simple to operate and short in time, so that the magnetic bead method for extracting nucleic acid is more and more frequently used in the field of medical detection.
The general steps for extracting nucleic acids by the magnetic bead method are lysis or conversion, washing and elution. At present, a plurality of steps for extracting nucleic acid by a magnetic bead method are generally carried out in different test tubes or kits, a plurality of test tubes are required to be prepared, samples are required to be continuously transferred in the extraction process, external pollution is easily introduced, the operation is more complicated on the other hand, and the extraction efficiency is lower.
SUMMERY OF THE UTILITY MODEL
The present invention aims to provide a nucleic acid extraction kit of the filter membrane type. When the kit is used for extracting nucleic acid of a sample, only a plurality of steps need to be completed in one kit, external pollution is not easy to introduce, and the extraction efficiency can be greatly improved.
The technical scheme of the utility model is as follows: the filtering membrane type nucleic acid extraction kit is characterized in that: the sample adding device comprises a box body, wherein a sample adding cavity and a liquid storage cavity are arranged on the box body, and a connecting liquid path is arranged between the sample adding cavity and the liquid storage cavity; the sample adding cavity is provided with an air hole and a sample adding hole, and the liquid storage cavity is provided with a liquid adding hole; and a filtering membrane is arranged at the joint of the sample adding cavity and the connecting liquid path.
In the above-mentioned filtration membrane-type nucleic acid extraction kit, the connecting liquid path is connected to the lower ends of the sample addition chamber and the liquid storage chamber, respectively.
In the filter membrane type nucleic acid extraction kit, the sample addition cavity and the liquid storage cavity are both obliquely guided at the bottom and connected with the liquid path.
Compared with the prior art, the kit provided by the utility model is respectively used as the sample adding cavity and the liquid storage cavity through the two cavities which are communicated by the connecting liquid path, the solution added into the liquid storage cavity can freely move between the two cavities by utilizing a pneumatic principle, the liquid transfer can be realized, meanwhile, the filtering membrane is used as a carrier for adsorbing nucleic acid in a combined manner, when the solution flows through the filtering membrane, the filtering membrane can adsorb the nucleic acid, and the steps of cleaning and eluting are carried out for one time or multiple times. Compared with a nucleic acid extraction kit adopting a magnetic bead method, the utility model does not need to separately design a magnetic bead adsorption mechanism, and has lower requirements on matched detection equipment.
Drawings
FIG. 1 is a schematic diagram of the construction of a kit according to the present invention;
fig. 2 is a schematic perspective view of the present invention.
Detailed Description
The utility model is further illustrated by the following figures and examples, which are not to be construed as limiting the utility model.
Example (b): a filtration membrane type nucleic acid extraction kit as shown in figure 1: the sample adding device comprises a box body 1, wherein a sample adding cavity 2 and a liquid storage cavity 3 are arranged on the box body 1, and a connecting liquid path 4 is arranged between the sample adding cavity 2 and the liquid storage cavity 3; the sample adding cavity 2 is provided with an air hole 21 and a sample adding hole 22, and the liquid storage cavity 3 is provided with a liquid adding hole 31; and a filtering membrane 23 is arranged at the joint of the sample adding cavity 2 and the connecting liquid path 4. And the connecting liquid path 4 is respectively connected with the lower ends of the sample adding cavity 2 and the liquid storage cavity 3. The bottom of the sample adding cavity 2 and the bottom of the liquid storage cavity 3 are inclined, guided and connected with a liquid path 4.
The extraction method using the filtering membrane type nucleic acid extraction kit comprises the following steps:
1. preparing materials: preparing the reagent kit and the filter membrane, and installing the filter membrane 23 according to the position shown in figure 1 to ensure that the contact part of the membrane and the box body 1 is tightly attached; the air hole 21 is connected with an air pump which can provide positive pressure and negative pressure; the bottom surface of the box body is pasted with a film to seal each liquid flow passage.
2. Adding a sample solution into the sample adding cavity 2, and sealing the sample adding hole 22; the binding liquid is added into the liquid storage cavity 3.
3. The air pump provides negative pressure, the binding solution is pumped into the sample adding cavity 2 to be mixed with the sample solution, and the mixture is kept stand for 5 min.
4. The air pump provides positive and negative pressure in a reciprocating manner to make the mixed solution pass through the filtering membrane back and forth, and the nucleic acid in the solution is adsorbed on the filtering membrane 23.
5. The solution is pumped into the reservoir 3 and removed by a pipette.
6. Cleaning fluid is added to the reservoir 3.
7. The air pump provides positive and negative pressure in a circulating reciprocating manner, so that the cleaning liquid passes through the filtering membrane 23 back and forth to clean redundant impurities.
8. The solution is pumped into the liquid storage cavity 3, removed by a liquid transfer device, and the desulfonation reagent is added into the liquid storage cavity 3.
9. The desulfonation reagent is applied to the filter membrane 23 and allowed to stand for 15 min.
10. The air pump provides positive and negative pressure in a reciprocating way, the solution is pumped into the liquid storage cavity 3, and the liquid storage cavity is removed by a liquid transfer device.
11. And repeating the steps 6 and 7 twice.
12. Eluent is added into the liquid storage cavity 3.
13. The air pump provides positive and negative pressure in a reciprocating way to make the eluent pass through the filtering membrane 23 back and forth, and finally the eluted solution is pumped into the liquid storage cavity 3 and extracted into a designated test tube by a liquid transfer device.
Claims (3)
1. Filtration membrane formula nucleic acid extraction kit, its characterized in that: comprises a box body (1), wherein a sample adding cavity (2) and a liquid storage cavity (3) are arranged on the box body (1), and a connecting liquid path (4) is arranged between the sample adding cavity (2) and the liquid storage cavity (3); the sample adding cavity (2) is provided with an air hole (21) and a sample adding hole (22), and the liquid storage cavity (3) is provided with a liquid adding hole (31); and a filtering membrane (23) is arranged at the joint of the sample adding cavity (2) and the connecting liquid path (4).
2. The filter membrane-type nucleic acid extraction kit according to claim 1, wherein: and the connecting liquid path (4) is respectively connected with the lower ends of the sample adding cavity (2) and the liquid storage cavity (3).
3. The filter membrane-type nucleic acid extraction kit according to claim 1, wherein: the bottom of each of the sample adding cavity (2) and the liquid storage cavity (3) is obliquely guided and connected with the liquid path (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122726972.7U CN216864151U (en) | 2021-11-09 | 2021-11-09 | Filtering membrane type nucleic acid extraction kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122726972.7U CN216864151U (en) | 2021-11-09 | 2021-11-09 | Filtering membrane type nucleic acid extraction kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216864151U true CN216864151U (en) | 2022-07-01 |
Family
ID=82126721
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122726972.7U Active CN216864151U (en) | 2021-11-09 | 2021-11-09 | Filtering membrane type nucleic acid extraction kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216864151U (en) |
-
2021
- 2021-11-09 CN CN202122726972.7U patent/CN216864151U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108660074B (en) | Integrated solution for nucleic acid extraction PCR amplification detection | |
| CN106999928B (en) | Nucleic acid purification box | |
| CN109456880B (en) | On-site rapid nucleic acid extraction tube and use method thereof | |
| CN109370896A (en) | Cartridge is used in a kind of closed four-way multiple fluorescence PCR detection | |
| CN209741123U (en) | Full-automatic nucleic acid extraction appearance | |
| CN216864151U (en) | Filtering membrane type nucleic acid extraction kit | |
| CN114989971A (en) | Card box device for nucleic acid extraction and automatic liquid separation and analysis method thereof | |
| CN114292742A (en) | Integrated exosome source nucleic acid extraction system and method based on digital microfluidic | |
| CN213295320U (en) | Reagent reaction box for nucleic acid extraction | |
| CN217499275U (en) | Totally-enclosed nucleic acid detection chip | |
| CN218339833U (en) | Liquid transfer, multi-channel liquid transfer, automatic liquid extraction and transfer and multi-channel automatic liquid extraction and transfer device | |
| CN215627905U (en) | Micro-fluidic chip for nucleic acid extraction | |
| CN111876313A (en) | Nucleic acid extraction device | |
| CN115747042A (en) | Full-automatic integrated nucleic acid extraction equipment for large-volume samples | |
| CN209260070U (en) | A kind of Novel free instrument nucleic acid on-site detection card | |
| CN214503472U (en) | High-flux full-automatic solid phase extraction instrument | |
| CN218318033U (en) | Kit component convenient for nucleic acid extraction and detection | |
| CN216614646U (en) | Nucleic acid extraction kit by magnetic bead method | |
| CN111893029A (en) | Nucleic acid extraction device including mixing device | |
| CN212222971U (en) | Nucleic acid extraction device | |
| CN207628021U (en) | Film making device for laboratory suction filtration | |
| CN207435462U (en) | A kind of hermetic multiple fluorescent PCR detection cartridge | |
| CN113930336A (en) | Nucleic acid extraction kit by magnetic bead method and extraction method thereof | |
| CN206232701U (en) | Magnetic frame with liquid suction function | |
| CN219385100U (en) | Reagent card box |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 225129 building 6, Chuangfu workshop, No. 1, Chuangfu Road, Hanjiang District, Yangzhou City, Jiangsu Province Patentee after: Jiangsu Fuyuan Biotechnology Co.,Ltd. Patentee after: HANGZHOU LIFEREAL BIOTECHNOLOGY Co.,Ltd. Patentee after: Jiangsu Huayuan Biotechnology Co.,Ltd. Patentee after: Hangzhou Suizeng Biotechnology Co.,Ltd. Address before: 225129 building 6, Chuangfu workshop, No. 1, Chuangfu Road, Hanjiang District, Yangzhou City, Jiangsu Province Patentee before: Jiangsu Fuyuan Biotechnology Co.,Ltd. Patentee before: HANGZHOU LIFEREAL BIOTECHNOLOGY Co.,Ltd. Patentee before: Shanghai Fuyuan Biotechnology Co.,Ltd. Patentee before: Hangzhou Suizeng Biotechnology Co.,Ltd. |
|
| CP01 | Change in the name or title of a patent holder |