CN214503472U - High-flux full-automatic solid phase extraction instrument - Google Patents
High-flux full-automatic solid phase extraction instrument Download PDFInfo
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- CN214503472U CN214503472U CN202022465829.2U CN202022465829U CN214503472U CN 214503472 U CN214503472 U CN 214503472U CN 202022465829 U CN202022465829 U CN 202022465829U CN 214503472 U CN214503472 U CN 214503472U
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Abstract
The utility model relates to the technical field of affinity columns, in particular to a high-flux full-automatic solid phase extraction instrument, which comprises an affinity column pretreatment all-in-one machine, wherein the affinity column pretreatment all-in-one machine comprises an injection pump, an X shaft assembly, a Y shaft assembly and a Z shaft assembly; a plurality of sensor induction discs are installed on the inner side of the injection pump, a screw motor is arranged above the sensor induction discs, a 12-way reversing valve is installed on the inner side of the injection pump, and the 12-way reversing valve is annularly arranged; the top of the front surface of the X shaft assembly is fixedly connected with a slide rail, the inner side of the slide rail is provided with a chain, two ends of the X shaft assembly are fixedly connected with trapezoidal plates, and a partition plate is fixedly connected between the two trapezoidal plates; the utility model discloses the primary function is to carry out automatic appearance of going up to the sample, then applys the malleation to the sample through the syringe pump, makes the sample can be through affinity post, and X axle subassembly, Y axle subassembly, Z axle subassembly realize the purpose that the sample was handled in batches to can accomplish the overall process of solid phase extraction automatically.
Description
Technical Field
The utility model relates to an affinity post technical field specifically is full-automatic solid phase extraction appearance of high flux.
Background
The affinity column is a column filled with an affinity adsorbent prepared by connecting a substance having a specific binding action with a purification target to an insoluble carrier. It is used for affinity chromatography. In the actual use process of the existing affinity column, the automatic processing capacity of the sample is poor, the use operation steps are complex, all the steps of solid-phase extraction cannot be effectively integrated on one platform, the full-automatic operation of the whole solid-phase extraction process (activation, loading, leaching, drying and elution) cannot be realized, and the efficiency of sample pretreatment is greatly reduced, so that the high-throughput full-automatic solid-phase extractor is provided.
SUMMERY OF THE UTILITY MODEL
The utility model provides a not enough to prior art, the utility model provides a high flux full-automatic solid phase extraction appearance possesses convenient extraction and purification to trace organic matter in the sample extract, is particularly suitable for the analysis of trace organic matter in the small volume liquid sample, is the sample pretreatment preparation system of gas phase, liquid chromatogram or mass spectrum instrument, and the whole pretreatment process of embedding that can be fine improves advantages such as efficiency of pretreatment, has solved the problem of carrying out automated processing that current high flux full-automatic solid phase extraction appearance can not be fine when using.
The utility model discloses a high flux full-automatic solid phase extraction appearance, including affinity post pretreatment all-in-one, this affinity post pretreatment all-in-one includes the syringe pump, X axle subassembly, Y axle subassembly and Z axle subassembly, a plurality of sensor response dishes are installed to the inboard of this syringe pump, the top of sensor response dish is equipped with the lead screw motor, 12-way reversing valves are installed to the inboard of this syringe pump, 12-way reversing valves are the cyclic annular arrangement setting, the positive top fixedly connected with slide rail of this X axle subassembly, the inboard of slide rail is installed the chain, and the both ends of X axle subassembly all fixedly connected with trapezoidal plate, the department fixedly connected with space bar between two trapezoidal plates, the back fixedly connected with driving motor of this Y axle subassembly, the below of driving motor is equipped with the syringe needle, the bottom of Y axle subassembly is equipped with the post inserted bar, the top fixedly connected with driving motor of this Z axle subassembly, the output shaft department of driving motor is equipped with the bearing board, a plurality of circular through holes are equidistantly formed in the top of the bearing plate, an injection tube is slidably connected to the circular through holes, a spring is arranged on the outer side of the injection tube, and the spring is located below the outer side of the injection tube.
The utility model discloses a full-automatic solid phase extraction appearance of high flux, wherein the bottom fixedly connected with chassis of syringe pump, two square groove have been seted up at the top on chassis, and this square groove punishment is fixedly connected with collection tray and the tray of taking a sample respectively.
The utility model discloses a full-automatic solid phase extraction appearance of high flux, wherein the circular through-hole has been seted up to the top equidistance of the tray that takes a sample, and the fixedly connected with setpoint of this circular through-hole department, the bottom fixedly connected with affinity post of this setpoint, and the equal fixedly connected with drive assembly in both sides of the tray that takes a sample.
The utility model discloses a full-automatic solid phase extraction appearance of high flux, wherein the top both ends difference fixedly connected with on chassis wash needle waste liquid groove and collect waste liquid groove, should wash needle waste liquid groove and collect waste liquid groove and be located the one side of collecting tray and the appearance tray of going up keeping away from each other.
The utility model discloses a full-automatic solid phase extraction appearance of high flux, wherein the left side of collecting the waste liquid groove is equipped with uncapping mechanism, and the top of this uncapping mechanism is installed and is had a tray, and the top of crossing tray and the top of the tray of getting on the same level are in same straight line department.
The utility model discloses a full-automatic solid phase extraction appearance of high flux, wherein the below of Z axle subassembly is equipped with nonrust steel pipe, the bottom and the washing needle waste liquid groove one-to-one of this nonrust steel pipe, and link to each other with the bottom of injection tube is fixed.
Compared with the prior art, the beneficial effects of the utility model are as follows:
the utility model discloses the primary function is to carry out automatic appearance of going up to the sample, then apply the malleation to the sample through the syringe pump, make the sample can pass through the affinity post, and X axle subassembly, Y axle subassembly, Z axle subassembly realizes the purpose of sample batch processing, and can accomplish the overall process of solid phase extraction automatically, can be used for food, medicine, beverage, blood, urine, soil, extraction and purification of trace organic matter in the sample extract such as water sample, be particularly suitable for the analysis of trace organic matter in the small volume liquid sample, be the sample pretreatment preparation system of gas phase, liquid chromatogram or mass spectrum instrument, the whole pretreatment process of embedding that can be fine, improve the efficiency of pretreatment, and the high flux full-automatic solid phase extraction appearance is with each effectual integration in a platform of step of solid phase extraction, can realize whole solid phase extraction process completely (activation, get the appearance, drip washing, the whole solid phase extraction process, Drying and elution) to greatly improve the efficiency of sample pretreatment, and release the analysis workers from the fussy pretreatment work to ensure that the sample pretreatment is quicker and more efficient, thereby reducing the interference of impurities in the sample on the detection and greatly improving the accuracy of the detection.
Drawings
The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the application and together with the description serve to explain the application and not to limit the application. In the drawings:
FIG. 1 is a schematic view of the three-dimensional structure of the affinity column pretreatment integrated machine of the present invention;
FIG. 2 is a schematic view of the three-dimensional structure of the injection pump of the present invention;
FIG. 3 is a schematic view of the three-dimensional structure of the X-axis assembly of the present invention;
FIG. 4 is a schematic view of the Y-axis assembly of the present invention;
fig. 5 is a schematic view of the three-dimensional structure of the sample loading tray of the present invention;
FIG. 6 is a schematic view of the Z-axis assembly of the present invention;
fig. 7 is a schematic view of the combined structure of the chassis, the sample loading tray and the collecting tray of the present invention.
In the figure: 01. an affinity column pretreatment integrated machine; 02. an injection pump; 03. an X-axis assembly; 04. a Y-axis assembly; 05. a chain; 06. a Z-axis assembly; 07. a chassis; 08. a stainless steel tube; 09. a post insert rod; 10. a sample loading tray; 11. a collection tray; 12. a column passing tray; 13. cleaning the needle waste liquid tank; 14. collecting a waste liquid tank; 15. a cap removal mechanism; 021. 12-way reversing valve; 022. a sensor sensing plate; 031. a trapezoidal plate; 032. a partition plate; 033. a slide rail; 041. a drive motor; 042. an injection needle; 101. positioning points; 102. an affinity column; 103. a transmission assembly; 061. a drive motor; 062. an injection tube; 063. a spring.
Detailed Description
In the following description, numerous implementation details are set forth in order to provide a more thorough understanding of the present invention. It should be understood, however, that these implementation details should not be used to limit the invention. That is, in some embodiments of the invention, details of these implementations are not necessary. In addition, some conventional structures and components are shown in simplified schematic form in the drawings.
In addition, the descriptions related to "first", "second", etc. in the present invention are only for description purposes, not specifically referring to the order or sequence, and are not intended to limit the present invention, but only to distinguish the components or operations described in the same technical terms, and are not to be construed as indicating or implying any relative importance or implicit indication of the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, the technical solutions in the embodiments may be combined with each other, but it must be based on the realization of those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should not be considered to exist, and is not within the protection scope of the present invention.
Referring to fig. 1-7, the high throughput full-automatic solid phase extractor of the present invention comprises an affinity column pretreatment integrated machine 01, wherein the affinity column pretreatment integrated machine 01 comprises an injection pump 02, an X-axis assembly 03, a Y-axis assembly 04 and a Z-axis assembly 06, a plurality of sensor discs 022 are mounted on the inner side of the injection pump 02, the sensor discs 022 are used for measuring the stepping accuracy of a motor, a lead screw motor is disposed above the sensor discs 022 and used for controlling the lifting of the injection pump 02, a 12-way directional valve 021 is mounted on the inner side of the injection pump 02, the 12-way directional valve 021 is annularly arranged, a slide rail 033 is fixedly connected to the top of the front side of the X-axis assembly 03, a chain 05 is mounted on the inner side of the slide rail 033, ladder plates 031 are fixedly connected to both ends of the X-axis assembly 03, a partition plate 032 is fixedly connected between the two ladder plates 031, a driving motor 041 is fixedly connected to the back side of the Y-axis assembly 04, an injection needle 042 is arranged below the driving motor 041, a column inserted bar 09 is arranged at the bottom of the Y-axis assembly 04, and the column inserted bar 09 is pressed in and can be compatible with various specifications, so that the trouble of replacing column inserted bars with different specifications is avoided; this Z axle subassembly 06's top fixedly connected with drive motor 061, drive motor 061's output shaft department is equipped with the bearing board, and a plurality of circular through-holes have been seted up to the top equidistance of this bearing board, and this circular through-hole department sliding connection has injection tube 062, and the outside of injection tube 062 is equipped with spring 063, and this spring 063 is located the outside below of injection tube 062.
The bottom of the injection pump 02 is fixedly connected with a chassis 07, the top of the chassis 07 is provided with two square grooves, and the square grooves are respectively and fixedly connected with a collection tray 11 and a sample loading tray 10.
Circular through holes are formed in the top of the sample loading tray 10 at equal intervals, positioning points 101 are fixedly connected to the circular through holes, affinity columns 102 are fixedly connected to the bottoms of the positioning points 101, and transmission assemblies 103 are fixedly connected to the two sides of the sample loading tray 10.
The two ends of the top of the chassis 07 are fixedly connected with a cleaning needle waste liquid tank 13 and a collecting waste liquid tank 14 respectively, and the cleaning needle waste liquid tank 13 and the collecting waste liquid tank 14 are positioned on one side of the collecting tray 11 and the sample loading tray 10 which are far away from each other.
The left side of the waste liquid collecting groove 14 is provided with a cap removing mechanism 15, the top of the cap removing mechanism 15 is provided with a column tray 12, and the top of the column tray 12 and the top of the sample loading tray 10 are positioned at the same horizontal straight line.
When using the utility model discloses the time:
the main function of the device is to automatically load the sample, and then apply positive pressure to the sample through the injection pump 02, so that the sample can be processed in batch through the affinity column 102, the X shaft assembly 03, the Y shaft assembly 04 and the Z shaft assembly 06, and the device has the following technical characteristics: the whole process of the solid phase extraction can be automatically completed and comprises column activation, sample loading, leaching, blow-drying, elution and step-by-step collection.
An extraction channel:
4 channels are designed to be amplified to 8 channels at most, flow rate and reagents can be independently controlled in each channel, 4 samples can be automatically processed at the same time, and simultaneous activation, simultaneous sampling and simultaneous elution of multiple channels are realized.
Capacity for continuous processing of samples:
48 samples can be continuously and automatically processed by using 1ml, 3ml and 6ml solid phase extraction columns; the immunoaffinity column 102 can be continuously and automatically processed, the caps of the immunoaffinity column 102 can be automatically removed by means of self mechanical action, at most 4 samples are continuously processed, the whole processing process does not need any manual intervention, and the full automation requirement is completely met.
Solvent and sample loading mode:
the 12-way reversing valve is used for automatically switching to select the solvent, the solvent and the sample respectively have independent pipelines, and all the pipelines are simultaneously connected with the multi-way valve, so that cross contamination caused by common pipelines of the sample and the solvent is avoided, frequent actions of the mechanical arm are reduced, and the sample processing speed is increased.
Sample treatment volume:
the sample loading has two specifications of a small volume sample of 12ml and a large volume sample of 60ml, and the simultaneous purification of 4 samples is met;
collecting:
4 samples meeting the maximum of 12ml and 60ml were collected simultaneously; 8 organic solvents are selected for activation and leaching, 8 solvent pipelines are independent, and the device has the function of automatically cleaning pipelines; a high-precision injection pump 02 is adopted, and the injection pump 02 is visualized, so that the precision can be conveniently observed and confirmed; sample loading flow rate: 0.1-100mL/min, elution and elution flow rate: 0.1-100 mL/min.
Column passing technology:
SPE post filler top is hugged closely to post inserted bar 09 bottom, and the air gap above the SPE post filler can be filled completely to post inserted bar 09, and the solvent directly gets into the extraction column and packs in, does not detain SPE plunger plate top, guarantees that the liquid velocity of flow of setting is the velocity of flow that liquid flowed through the SPE post promptly.
Sealing the column requires:
the elastic O-shaped ring and the SPE are sealed at the low point of the extraction plunger plate, and the non-sealing cover and the SPE column port are sealed, so that the solvent mixing phenomenon is avoided, and the liquid leakage problem is avoided; the column inserted rod 09 can be compatible with 1mL, 3mL and 6mL of three immunoaffinity columns 102 at the same time, so that the trouble of replacing the column inserted rod 09 is avoided; three groups of independent mechanical arms are arranged to realize the movement of a sample loading mechanism, a column inserted link 09 mechanism and an extraction column frame, an inclined waste discharge groove, an independent sample loading frame, a collection frame and a column passing frame, so that four functions of sample loading, column passing, collection and waste discharge can be ensured to be operated independently without mutual interference; sample frame, collection frame, SPE post frame, the groove of wasting discharge possess automatic positioning function, and automatic discernment, removal are located to the target, and synchronous operation mutual noninterference, compatible three kinds of 1ml, 3ml, 6ml solid phase extraction columella can place 48 simultaneously.
The active waste discharge function:
4 independent waste discharge channels can separately recycle and treat waste water, waste organic solvent, highly toxic waste liquid and needle washing waste liquid.
The above are merely embodiments of the present invention, and are not intended to limit the present invention. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the scope of the claims of the present invention.
Claims (6)
1. Full-automatic solid phase extraction appearance of high flux, including affinity post pretreatment all-in-one (01), its characterized in that: the affinity column pretreatment integrated machine (01) comprises an injection pump (02), an X shaft assembly (03), a Y shaft assembly (04) and a Z shaft assembly (06);
a plurality of sensor induction discs (022) are installed on the inner side of the injection pump (02), a screw motor is arranged above the sensor induction discs (022), a 12-way reversing valve (021) is installed on the inner side of the injection pump (02), and the 12-way reversing valve (021) is annularly arranged;
the top of the front face of the X-axis assembly (03) is fixedly connected with a slide rail (033), the inner side of the slide rail (033) is provided with a chain (05), two ends of the X-axis assembly (03) are fixedly connected with trapezoidal plates (031), and a partition plate (032) is fixedly connected between the two trapezoidal plates (031);
the back of the Y-axis component (04) is fixedly connected with a driving motor (041), an injection needle (042) is arranged below the driving motor (041), and the bottom of the Y-axis component (04) is provided with a column inserted rod (09);
the top fixedly connected with drive motor (061) of this Z axle subassembly (06), the output shaft department of drive motor (061) is equipped with the bearing board, and a plurality of circular through-holes have been seted up to the top equidistance of this bearing board, and this circular through-hole department sliding connection has injection tube (062), and the outside of injection tube (062) is equipped with spring (063), and this spring (063) is located the outside below of injection tube (062).
2. The high throughput full-automatic solid phase extractor of claim 1, wherein: the bottom of the injection pump (02) is fixedly connected with a chassis (07), the top of the chassis (07) is provided with two square grooves, and the square grooves are respectively and fixedly connected with a collection tray (11) and a sample loading tray (10).
3. The high throughput full-automatic solid phase extractor of claim 2, wherein: circular through holes are formed in the top of the sample loading tray (10) at equal intervals, locating points (101) are fixedly connected to the circular through holes, affinity columns (102) are fixedly connected to the bottoms of the locating points (101), and transmission assemblies (103) are fixedly connected to the two sides of the sample loading tray (10).
4. The high throughput full-automatic solid phase extractor of claim 2, wherein: and two ends of the top of the chassis (07) are respectively and fixedly connected with a cleaning needle waste liquid tank (13) and a collecting waste liquid tank (14), and the cleaning needle waste liquid tank (13) and the collecting waste liquid tank (14) are positioned on one side of the collecting tray (11) and the sample loading tray (10) which are far away from each other.
5. The high-throughput full-automatic solid phase extractor of claim 4, wherein: the left side of the waste liquid collecting groove (14) is provided with a cap removing mechanism (15), the top of the cap removing mechanism (15) is provided with a column passing tray (12), and the top of the column passing tray (12) and the top of the sample loading tray (10) are positioned at the same horizontal straight line.
6. The high throughput full-automatic solid phase extractor of claim 1, wherein: and a stainless steel pipe (08) is arranged below the Z shaft component (06), the bottoms of the stainless steel pipes (08) correspond to the cleaning needle waste liquid grooves (13) one by one, and are fixedly connected with the bottom of the injection cylinder (062).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202022465829.2U CN214503472U (en) | 2020-10-30 | 2020-10-30 | High-flux full-automatic solid phase extraction instrument |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202022465829.2U CN214503472U (en) | 2020-10-30 | 2020-10-30 | High-flux full-automatic solid phase extraction instrument |
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| CN214503472U true CN214503472U (en) | 2021-10-26 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115645986A (en) * | 2022-12-27 | 2023-01-31 | 河北华清旭隆智能设备有限公司 | Full-automatic solid phase extraction instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115645986A (en) * | 2022-12-27 | 2023-01-31 | 河北华清旭隆智能设备有限公司 | Full-automatic solid phase extraction instrument |
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