CN215627905U

CN215627905U - Micro-fluidic chip for nucleic acid extraction

Info

Publication number: CN215627905U
Application number: CN202122161391.3U
Authority: CN
Inventors: 王鹏
Original assignee: Genfine Biotech Beijing Co ltd
Current assignee: Genfine Biotech Beijing Co ltd
Priority date: 2021-09-08
Filing date: 2021-09-08
Publication date: 2022-01-25
Anticipated expiration: 2031-09-08

Abstract

The utility model discloses a micro-fluidic chip for nucleic acid extraction, which comprises a lysate sample reaction cavity, a first washing tank and a second washing tank, wherein one end of a first micro-fluidic pipeline, which is far away from the lysate sample reaction cavity, is communicated with a filtering device, the first washing tank is communicated with a second micro-fluidic pipeline, one end of the second micro-fluidic pipeline, which is far away from the first washing tank, is communicated with the filtering device, the outside of the second washing tank is communicated with a third micro-fluidic pipeline, one end of the third micro-fluidic pipeline, which is far away from the second washing tank, is communicated with the filtering device, the outside of an eluent storage tank is communicated with a fourth micro-fluidic pipeline, the liquid in the chip flows to perform nucleic acid extraction by matching a vacuum pump filtration principle with the filtering device and a filtering membrane to adsorb nucleic acid, so that the problem of alcohol residue drying in the nucleic acid extraction process is avoided, the efficiency of nucleic acid adsorption in the adsorption device is high, the time for extracting nucleic acid is reduced, and the efficiency of extracting nucleic acid is improved.

Description

Micro-fluidic chip for nucleic acid extraction

Technical Field

The utility model relates to the technical field of nucleic acid extraction, in particular to a micro-fluidic chip for nucleic acid extraction.

Background

The microfluidic chip achieves the functions of the whole chemical and biological laboratory by accurately manipulating and controlling microfluid in a microchannel network, and is also called as a 'lab-on-a-chip'. In the current microfluidic nucleic acid extraction, a magnetic bead method is adopted for nucleic acid extraction in most chip extraction methods, and the magnetic bead method has the problems that after rinsing, magnetic beads cannot be dried in the air, so that alcohol substance residues exist, downstream PCR amplification inhibition is affected, the flowing speed of liquid in a micro-pipeline is slow, the time for nucleic acid extraction is 1-2 times that of a conventional method, and the working efficiency is not high.

SUMMERY OF THE UTILITY MODEL

In view of the problems mentioned in the background, it is an object of the present invention to provide a microfluidic chip for nucleic acid extraction to solve the problems mentioned in the background.

The technical purpose of the utility model is realized by the following technical scheme:

a micro-fluidic chip for nucleic acid extraction comprises a lysate sample reaction cavity, a first washing liquid tank, a second washing liquid tank, an eluent storage tank and a filtering device, wherein a first micro-flow pipeline is communicated with the lysate sample reaction cavity, one end, far away from the lysate sample reaction cavity, of the first micro-flow pipeline is communicated with the filtering device, a second micro-flow pipeline is communicated with the first washing liquid tank, one end, far away from the first washing liquid tank, of the second micro-flow pipeline is communicated with the filtering device, a third micro-flow pipeline is communicated with the outside of the second washing liquid tank, one end, far away from the second washing liquid tank, of the third micro-flow pipeline is communicated with the filtering device, a fourth micro-flow pipeline is communicated with the outside of the eluent storage tank, one end, far away from the eluent storage tank, of the fourth micro-flow pipeline is communicated with the filtering device, one side of filter equipment is provided with the vacuum pump, the vacuum pump is kept away from one side of filter equipment is provided with the negative pressure suction filtration jar, the vacuum pump respectively with filter equipment with the negative pressure suction filtration jar is linked together, the outside intercommunication of negative pressure suction filtration jar has fifth miniflow pipeline, fifth miniflow pipeline is kept away from the one end intercommunication of negative pressure suction filtration jar has the waste liquid chamber, the outside intercommunication of negative pressure suction filtration jar has sixth miniflow pipeline, sixth miniflow pipeline is kept away from the one end intercommunication of negative pressure suction filtration jar has the eluant to collect the chamber, the outside intercommunication in eluant collection chamber has seventh miniflow pipeline, seventh miniflow pipeline is kept away from the one end intercommunication in eluant collection chamber has polymerase chain reaction cauldron.

Through adopting above-mentioned technical scheme, in the middle of adding the lysate sample reaction chamber with the sample that will carry out the schizolysis, carry out the misce bene, the reaction is accomplished the back and is started the vacuum pump and filter suction filtration liquid through in the middle of first miniflow pipeline suction filter equipment, the waste liquid is carried into in the middle of the waste liquid chamber through fifth miniflow pipeline simultaneously, and first lotion jar, be rinsing liquid in the second lotion jar, and the sample in the eluent storage jar can be detached and washed, the sample in first lotion jar and the second lotion jar finally can be through in the middle of the pipeline backward flow goes into the waste liquid chamber.

Preferably, a filter screen is arranged in the negative pressure suction filtration tank.

By adopting the technical scheme, the filter screen in the negative pressure suction filtration tank is used for filtering the sample to adsorb the nucleic acid.

Preferably, an air inlet of the vacuum pump is communicated with the inside of the filtering device, and an air outlet of the vacuum pump is communicated with the inside of the negative pressure pumping and filtering tank.

Through adopting above-mentioned technical scheme, the air inlet and the filter equipment intercommunication of vacuum pump, the vacuum pump of being convenient for forms the negative pressure to filter equipment is inside.

Preferably, the waste liquid cavity is a square waste liquid collecting box, and the eluent collecting cavity is a barrel-shaped eluent collecting barrel.

Through adopting above-mentioned technical scheme, the waste liquid chamber is used for collecting and saving the waste liquid for square waste liquid collecting box, and the eluant collecting chamber is that tubbiness eluant collecting vessel is used for collecting the eluant.

Preferably, the second microfluidic channel and the third microfluidic channel have equal channel lengths and channel diameters.

By adopting the technical scheme, the lengths and diameters of the second microflow pipeline and the third microflow pipeline are equal, so that equal amount of liquid in the first washing liquid tank and the second washing liquid tank can be conveniently conveyed.

Preferably, the volumes and volumes of the lysate sample reaction chamber, the first washing solution tank, the second washing solution tank and the eluent storage tank are all equal.

By adopting the technical scheme, the volumes and the volumes of the lysate sample reaction cavity, the first washing solution tank, the second washing solution tank and the eluent storage tank are equal, so that the same amount of liquid samples can be conveniently stored.

In summary, the utility model mainly has the following beneficial effects:

in this micro-fluidic chip of nucleic acid extraction, the liquid flow utilizes vacuum pump negative pressure suction filtration principle cooperation filter equipment and filtration membrane absorption nucleic acid to carry out nucleic acid extraction in the chip, avoids the problem that alcohol residue dries in the air among the nucleic acid extraction process, attaches nucleic acid efficiency in the absorption device higher, reduces the time of nucleic acid extraction, improves the efficiency of nucleic acid extraction.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

fig. 2 is a schematic top view of the present invention.

Reference numerals: 10. a lysate sample reaction chamber; 11. a first liquid washing tank; 12. a second liquid washing tank; 13. an eluent storage tank; 14. a filtration device; 15. a vacuum pump; 16. a negative pressure suction filtration tank; 17. a waste fluid chamber; 18. an eluent collection cavity; 19. a polymerase chain reaction kettle; 20. a first microfluidic conduit; 21. a second microfluidic conduit; 22. a third microfluidic conduit; 23. a fourth microfluidic conduit; 24. a fifth microfluidic channel; 25. a sixth microfluidic channel; 26. a seventh microfluidic channel.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1 and 2, a microfluidic chip for nucleic acid extraction includes a lysate sample reaction chamber 10, a first eluent tank 11, a second eluent tank 12, an eluent storage tank 13 and a filtering device 14, wherein the volumes of the lysate sample reaction chamber 10, the first eluent tank 11, the second eluent tank 12 and the eluent storage tank 13 are equal, the first eluent tank 11 and the second eluent tank 12 are used for placing a lysate sample, the eluent storage tank 13 is used for storing an eluent sample, the filtering device 14 is provided with a filtering membrane, and the filtering membrane is used for adsorbing nucleic acid and extracting nucleic acid;

wherein, the exterior of the first washing liquid tank 11 and the second washing liquid tank 12 is respectively communicated with a second micro-flow pipeline 21 and a third micro-flow pipeline 22, the other ends of the second micro-flow pipeline 21 and the third micro-flow pipeline 22 are both connected with a filtering device 14, the exterior of the lysis liquid sample reaction chamber 10 and the exterior of the eluent storage tank 13 are respectively communicated with a first micro-flow pipeline 20 and a fourth micro-flow pipeline 23, one ends of the first micro-flow pipeline 20 and the fourth micro-flow pipeline 23 far away from the lysis liquid sample reaction chamber 10 and the eluent storage tank 13 are both communicated with the filtering device 14, one side of the filtering device 14 is provided with a vacuum pump 15, one side of the vacuum pump 15 far away from the filtering device 14 is provided with a negative pressure suction filtration tank 16, the air inlet of the vacuum pump 15 is communicated with the filtering device 14, the air outlet of the vacuum pump 15 is connected with the negative pressure suction filtration tank 16, one side of the negative pressure suction filtration tank 16 is respectively provided with a waste liquid cavity 17, an eluent collection cavity 18 and a polymerase chain reaction kettle 19, the polymerase chain reaction kettle 19 is connected with the eluent collecting cavity 18 through a seventh microflow pipeline 26, the eluent collecting cavity 18 is communicated with the negative pressure suction filtration tank 16 through a sixth microflow pipeline 25, and the negative pressure suction filtration tank 16 is communicated with the waste liquid cavity 17 through a fifth microflow pipeline 24

The working principle is as follows: firstly, cracking a sample, adding the sample to be cracked into a cracking liquid sample reaction cavity 10, uniformly mixing, starting a vacuum pump 15 to perform negative pressure suction filtration on a filter device 14 after the reaction is finished, simultaneously conveying waste liquid into a waste liquid cavity 17 through a fifth microflow pipeline 24, rinsing the sample in the second step, conveying a first washing liquid tank 11 into the filter device 14 through a second microflow pipeline 21, standing the sample in the filter device 14 for several minutes, filtering through a filter membrane, conveying the waste liquid into the waste liquid cavity 17 through a negative pressure suction filtration tank 16, conveying the washing liquid into the filter device 14 through a second washing liquid tank 12 and a third pipeline 22 by using a washing liquid sample in the second washing liquid tank 12 in the third step, conveying the waste liquid into the waste liquid cavity 17 through a negative pressure suction filtration tank 16, conveying the eluent 13 into the filter device 14 through a fourth washing liquid pipeline 23 in the fourth step, standing for several minutes, filtering by a filter membrane, and conveying the liquid into the eluent collection cavity 18 through a sixth microflow pipeline 25 to finish the extraction process.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a micro-fluidic chip for nucleic acid extraction, includes lysate sample reaction chamber (10), first lotion jar (11), second lotion jar (12), eluent storage jar (13) and filter equipment (14), its characterized in that: a first micro-flow pipeline (20) is communicated with the lysate sample reaction cavity (10), one end, far away from the lysate sample reaction cavity (10), of the first micro-flow pipeline (20) is communicated with the filtering device (14), a second micro-flow pipeline (21) is communicated with the first liquid washing tank (11), one end, far away from the first liquid washing tank (11), of the second micro-flow pipeline (21) is communicated with the filtering device (14), a third micro-flow pipeline (22) is communicated with the outside of the second liquid washing tank (12), one end, far away from the second liquid washing tank (12), of the third micro-flow pipeline (22) is communicated with the filtering device (14), a fourth micro-flow pipeline (23) is communicated with the outside of the eluent storage tank (13), one end, far away from the eluent storage tank (13), of the fourth micro-flow pipeline (23) is communicated with the filtering device (14), a vacuum pump (15) is arranged on one side of the filtering device (14), a negative pressure pumping and filtering tank (16) is arranged on one side of the vacuum pump (15) far away from the filtering device (14), the vacuum pump (15) is respectively communicated with the filtering device (14) and the negative pressure pumping and filtering tank (16), the outside of the negative pressure suction filtration tank (16) is communicated with a fifth microflow pipeline (24), one end of the fifth micro-flow pipeline (24) far away from the negative pressure suction filtration tank (16) is communicated with a waste liquid cavity (17), a sixth microflow pipeline (25) is communicated with the outside of the negative pressure suction filtration tank (16), an eluent collecting cavity (18) is communicated with one end of the sixth microflow pipeline (25) far away from the negative pressure suction filtration tank (16), the exterior of the eluent collecting cavity (18) is communicated with a seventh microflow pipeline (26), one end of the seventh microflow pipeline (26) far away from the eluent collection cavity (18) is communicated with a polymerase chain reaction kettle (19).

2. The microfluidic chip for nucleic acid extraction according to claim 1, wherein: a filter screen is arranged in the negative pressure suction filtration tank (16).

3. The microfluidic chip for nucleic acid extraction according to claim 1, wherein: the air inlet of the vacuum pump (15) is communicated with the inside of the filtering device (14), and the air outlet of the vacuum pump (15) is communicated with the inside of the negative pressure pumping and filtering tank (16).

4. The microfluidic chip for nucleic acid extraction according to claim 1, wherein: the waste liquid cavity (17) is a square waste liquid collecting box, and the eluent collecting cavity (18) is a barrel-shaped eluent collecting barrel.

5. The microfluidic chip for nucleic acid extraction according to claim 4, wherein: the second microfluidic channel (21) and the third microfluidic channel (22) have the same channel length and channel diameter.

6. The microfluidic chip for nucleic acid extraction according to claim 5, wherein: the volumes and the volumes of the lysate sample reaction cavity (10), the first washing solution tank (11), the second washing solution tank (12) and the eluent storage tank (13) are equal.